DK1225228T3 - Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle - Google Patents

Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle

Info

Publication number
DK1225228T3
DK1225228T3 DK01130275T DK01130275T DK1225228T3 DK 1225228 T3 DK1225228 T3 DK 1225228T3 DK 01130275 T DK01130275 T DK 01130275T DK 01130275 T DK01130275 T DK 01130275T DK 1225228 T3 DK1225228 T3 DK 1225228T3
Authority
DK
Denmark
Prior art keywords
laser beam
foreign material
living cells
feeding
feeding foreign
Prior art date
Application number
DK01130275T
Other languages
English (en)
Inventor
Akio Kobayashi
Kiichi Fukui
Satoshi Harajima
Eiichiro Fukusaki
Shinichiro Kajiyama
Takeshi Shoji
Shinya Okuda
Original Assignee
Univ Osaka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Osaka filed Critical Univ Osaka
Application granted granted Critical
Publication of DK1225228T3 publication Critical patent/DK1225228T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Electromagnetism (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DK01130275T 2000-12-25 2001-12-20 Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle DK1225228T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000392167 2000-12-25
JP2001349559A JP2002325572A (ja) 2000-12-25 2001-11-15 外来物質の導入方法

Publications (1)

Publication Number Publication Date
DK1225228T3 true DK1225228T3 (da) 2005-11-07

Family

ID=26606486

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01130275T DK1225228T3 (da) 2000-12-25 2001-12-20 Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle

Country Status (6)

Country Link
US (1) US7132289B2 (da)
EP (1) EP1225228B1 (da)
JP (1) JP2002325572A (da)
AU (1) AU773342B2 (da)
DE (1) DE60112398T2 (da)
DK (1) DK1225228T3 (da)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6514722B2 (en) 1997-03-27 2003-02-04 Oncosis Method and apparatus for selectively targeting specific cells within a cell population
US6753161B2 (en) * 1997-03-27 2004-06-22 Oncosis Llc Optoinjection methods
PT1295611E (pt) * 2000-06-20 2010-09-14 Dainippon Sumitomo Pharma Co Preparações para transferência de oligonucleótidos
EP1344181B1 (en) 2000-10-24 2013-12-11 Intrexon Corporation Method and device for selectively targeting cells within a three -dimensional specimen
CN1313158C (zh) 2001-06-20 2007-05-02 大日本住友制药株式会社 促进核酸转移的方法
JP4022614B2 (ja) * 2002-03-25 2007-12-19 国立大学法人大阪大学 新規なバイオビーズの作製方法
US6863406B2 (en) * 2002-08-01 2005-03-08 The University Of Chicago Apparatus and method for fabricating, sorting, and integrating materials with holographic optical traps
US7361171B2 (en) 2003-05-20 2008-04-22 Raydiance, Inc. Man-portable optical ablation system
US8921733B2 (en) 2003-08-11 2014-12-30 Raydiance, Inc. Methods and systems for trimming circuits
US9022037B2 (en) 2003-08-11 2015-05-05 Raydiance, Inc. Laser ablation method and apparatus having a feedback loop and control unit
US8173929B1 (en) 2003-08-11 2012-05-08 Raydiance, Inc. Methods and systems for trimming circuits
US20050095578A1 (en) * 2003-10-31 2005-05-05 Koller Manfred R. Method and apparatus for cell permeabilization
EP1696034A4 (en) * 2003-12-19 2006-12-27 Dainippon Sumitomo Pharma Co NUCLEIC ACID TRANSFER METHOD
US7425426B2 (en) 2004-03-15 2008-09-16 Cyntellect, Inc. Methods for purification of cells based on product secretion
JP4504082B2 (ja) 2004-04-28 2010-07-14 富士通株式会社 液体注入装置
JP2006006223A (ja) * 2004-06-25 2006-01-12 Canon Inc 微粒子の細胞への導入と回収装置、及びその導入と細胞あるいは細胞内の標的生体分子の回収方法
US20090062184A1 (en) * 2005-03-24 2009-03-05 Dainippon Sumitomo Pharma Co., Ltd. Fine particulate preparation comprising complex of nucleic acid molecule and collagen
US8135050B1 (en) 2005-07-19 2012-03-13 Raydiance, Inc. Automated polarization correction
ES2433669T3 (es) * 2005-12-13 2013-12-12 The Trustees Of The University Of Pennsylvania Métodos para fototransfectar ácido nucleico en células vivas
US10647960B2 (en) 2005-12-13 2020-05-12 The Trustees Of The University Of Pennsylvania Transcriptome transfer produces cellular phenotype conversion
US9157066B2 (en) 2005-12-13 2015-10-13 The Trustees Of The University Of Pennsylvania Transcriptome transfer produces cellular phenotype conversion
WO2007076458A1 (en) * 2005-12-21 2007-07-05 Primegen Biotech Llc Microinjector chip
US8189971B1 (en) 2006-01-23 2012-05-29 Raydiance, Inc. Dispersion compensation in a chirped pulse amplification system
US7444049B1 (en) 2006-01-23 2008-10-28 Raydiance, Inc. Pulse stretcher and compressor including a multi-pass Bragg grating
US8232687B2 (en) 2006-04-26 2012-07-31 Raydiance, Inc. Intelligent laser interlock system
US7822347B1 (en) 2006-03-28 2010-10-26 Raydiance, Inc. Active tuning of temporal dispersion in an ultrashort pulse laser system
EP2169070B1 (en) * 2007-06-21 2015-06-10 National University Corporation Nagoya University Method for introducing foreign substance into cell having cell wall
JP2009038988A (ja) * 2007-08-06 2009-02-26 Hiroshima Pref Gov 辛味成分の少ないアリウム属植物の栽培方法
WO2009140701A2 (en) * 2008-05-16 2009-11-19 Synthetic Genomics, Inc. Delivery into cells using ultra-short pulse lasers
BRPI0917993B8 (pt) * 2008-08-14 2021-05-25 Mesoblast Int Sarl composições de célula-tronco mesenquimal purificada
US8125704B2 (en) 2008-08-18 2012-02-28 Raydiance, Inc. Systems and methods for controlling a pulsed laser by combining laser signals
US20100055756A1 (en) * 2008-08-28 2010-03-04 Gregory Spooner System and method for modifying biological cells using an ultra-short pulsed laser
JP2012514477A (ja) * 2009-01-09 2012-06-28 イントレクソン コーポレイション 細胞の遺伝学的解析
WO2010081171A2 (en) 2009-01-12 2010-07-15 Cyntellect, Inc. Laser mediated sectioning and transfer of cell colonies
EP2413906B1 (en) 2009-03-31 2017-03-15 The Board of Trustees of the University of Arkansas Method of controlled drug release from a liposome carrier
WO2012021748A1 (en) 2010-08-12 2012-02-16 Raydiance, Inc. Polymer tubing laser micromachining
US9114482B2 (en) 2010-09-16 2015-08-25 Raydiance, Inc. Laser based processing of layered materials
JP6219832B2 (ja) 2011-10-17 2017-10-25 マサチューセッツ インスティテュート オブ テクノロジー 細胞内送達
US9849087B2 (en) 2011-11-08 2017-12-26 The Board Of Trustees Of The University Of Arkansas Methods and compositions for X-ray induced release from pH sensitive liposomes
US9485953B2 (en) * 2012-07-19 2016-11-08 R.J. Reynolds Tobacco Company Method for treating tobacco plants with enzymes
AU2014306423B2 (en) 2013-08-16 2019-04-18 Massachusetts Institute Of Technology Selective delivery of material to cells
US9265260B1 (en) 2014-04-07 2016-02-23 Gpd Technologies Llc Systems and methods for using light energy to facilitate penetration of substances in plants
US10278334B2 (en) 2014-04-07 2019-05-07 Premier Citrus Apz, Llc Systems and methods for delivering nucleic acids to a plant
US11178823B2 (en) 2014-04-07 2021-11-23 Premier Citrus Apz, Llc Systems and methods for using light energy to facilitate penetration of substances in plants
KR20170074235A (ko) 2014-10-31 2017-06-29 메사추세츠 인스티튜트 오브 테크놀로지 면역 세포로의 생체분자의 전달
CN113897285A (zh) 2014-11-14 2022-01-07 麻省理工学院 化合物和组合物向细胞中的破坏和场实现的递送
US11125739B2 (en) 2015-01-12 2021-09-21 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
CA2988996A1 (en) 2015-07-09 2017-01-12 Massachusetts Institute Of Technology Delivery of materials to anucleate cells
EP3344575B1 (en) * 2015-09-04 2020-04-15 SQZ Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
CU24681B1 (es) * 2015-12-10 2023-10-06 Premier Citruz Apz Llc Método y sistema para el tratamiento de plantas con huanglongbing (hlb)
US11191278B2 (en) 2016-03-25 2021-12-07 Premier Citrus Apz, Llc Systems and methods for delivering nucleic acids to a plant
CN111879434B (zh) * 2020-07-24 2022-04-08 之江实验室 一种测量生物组织或细胞温度的方法及装置
EP3971295A1 (en) * 2020-09-16 2022-03-23 FRAUNHOFER-GESELLSCHAFT zur Förderung der angewandten Forschung e.V. Methods for the production of genome edited plants
EP3971296A1 (en) * 2020-09-16 2022-03-23 FRAUNHOFER-GESELLSCHAFT zur Förderung der angewandten Forschung e.V. Methods for producing transformed plants

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US495050A (en) * 1893-04-11 Multiple-boring device
JPS6083583A (ja) 1983-10-13 1985-05-11 Rikagaku Kenkyusho 生細胞レ−ザ−穿孔装置
EP0137504B1 (en) 1983-10-13 1991-01-16 Rikagaku Kenkyusho Method and apparatus of implanting living cells with a foreign substance
JPS6083584A (ja) 1983-10-13 1985-05-11 Rikagaku Kenkyusho 生細胞内への物質移入法
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
GB8806643D0 (en) 1988-03-21 1988-04-20 Ici Plc Genetic manipulation
AU643563B2 (en) 1990-11-01 1993-11-18 Sapporo Breweries Limited Method for preparing transformed plant
US6346101B1 (en) * 1993-07-19 2002-02-12 Research Foundation Of City College Of New York Photon-mediated introduction of biological materials into cells and/or cellular components
JPH09163984A (ja) 1995-10-12 1997-06-24 Sony Corp 遺伝子組替え用レーザ装置およびこれを用いた遺伝子組替え方法
US5916788A (en) * 1995-10-12 1999-06-29 Sony Corporation Genetic recombination laser apparatus and genetic recombination method using the apparatus

Also Published As

Publication number Publication date
DE60112398T2 (de) 2005-12-29
US7132289B2 (en) 2006-11-07
JP2002325572A (ja) 2002-11-12
EP1225228A2 (en) 2002-07-24
US20020115219A1 (en) 2002-08-22
DE60112398D1 (de) 2005-09-08
EP1225228A3 (en) 2002-11-13
AU773342B2 (en) 2004-05-20
AU9729001A (en) 2002-06-27
EP1225228B1 (en) 2005-08-03

Similar Documents

Publication Publication Date Title
DK1225228T3 (da) Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle
GB9906291D0 (en) Method of etching transpant solid material with laser beam
GB2391506B (en) Method of severing an optical fibre using a laser beam
DE19983939T1 (de) Laserstrahlmaschine
FI20010567A (fi) Lattiapalkki
DE50115520D1 (de) Strahlformungsverfahren
NO981919D0 (no) FremgangsmÕte ved fremstilling av hydrogen ved bruk av et fast elektrolyttmembran
EP1310321A4 (en) LASER BEAM MACHINING PROCESS
DE69930194D1 (de) Brennstoffzellenanlage
DK1394929T3 (da) Fremgangsmåde til drift af et vindenergianlæg og vindenergianlæg
FR2778182B1 (fr) Procede de fabrication de carbonate de glycerol
DK94695A (da) Fremgangsmåde til generering af elektrisk energi
FI20002096A0 (fi) Menetelmä sähköverkon vikaantumassa olevan tai viallisen lähdön tai haaran ilmaisemiseksi
DK0919619T4 (da) Optimering af celler til endogen genaktivering
DK1263070T3 (da) Fremgangsmåde til forbedring af ydelsen af en brændstofcelle til direkte brændstoftilførsel
FR2779009B1 (fr) Sequencage automatique d'operations de focalisation de faisceaux ioniques
DE69924544D1 (de) Faserlaser
DE69940041D1 (de) Festelektrolytbrennstoffzelle
FR2787013B1 (fr) Prothese totale metatarso-phalangienne a coupe
FI19992005A (fi) Menetelmä ehdokassolun määrittämiseksi aktiivijoukkoon
EP1179105A4 (en) METHOD FOR PRODUCING PRE-BENDED BEAMS
DK0661767T3 (da) Fremgangsmåde til fremstilling af katoder til brændselsceller
FR2812317B1 (fr) Poutre creuse et procede de fabrication de ladite poutre
DE69801726D1 (de) Hochleistungslaser
ITRM990313A0 (it) Metodo di saldatura a fascio laser.