DK1225228T3 - Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle - Google Patents

Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle

Info

Publication number
DK1225228T3
DK1225228T3 DK01130275T DK01130275T DK1225228T3 DK 1225228 T3 DK1225228 T3 DK 1225228T3 DK 01130275 T DK01130275 T DK 01130275T DK 01130275 T DK01130275 T DK 01130275T DK 1225228 T3 DK1225228 T3 DK 1225228T3
Authority
DK
Denmark
Prior art keywords
laser beam
foreign material
living cells
feeding
feeding foreign
Prior art date
Application number
DK01130275T
Other languages
English (en)
Inventor
Akio Kobayashi
Kiichi Fukui
Satoshi Harajima
Eiichiro Fukusaki
Shinichiro Kajiyama
Takeshi Shoji
Shinya Okuda
Original Assignee
Univ Osaka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Osaka filed Critical Univ Osaka
Application granted granted Critical
Publication of DK1225228T3 publication Critical patent/DK1225228T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Electromagnetism (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DK01130275T 2000-12-25 2001-12-20 Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle DK1225228T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000392167 2000-12-25
JP2001349559A JP2002325572A (ja) 2000-12-25 2001-11-15 外来物質の導入方法

Publications (1)

Publication Number Publication Date
DK1225228T3 true DK1225228T3 (da) 2005-11-07

Family

ID=26606486

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01130275T DK1225228T3 (da) 2000-12-25 2001-12-20 Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle

Country Status (6)

Country Link
US (1) US7132289B2 (da)
EP (1) EP1225228B1 (da)
JP (1) JP2002325572A (da)
AU (1) AU773342B2 (da)
DE (1) DE60112398T2 (da)
DK (1) DK1225228T3 (da)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6753161B2 (en) * 1997-03-27 2004-06-22 Oncosis Llc Optoinjection methods
US6514722B2 (en) 1997-03-27 2003-02-04 Oncosis Method and apparatus for selectively targeting specific cells within a cell population
US20040052840A1 (en) * 2000-06-20 2004-03-18 Shunichiro Kubota Preparations for oligonucleotide transfer
US6804385B2 (en) 2000-10-24 2004-10-12 Oncosis Method and device for selectively targeting cells within a three-dimensional specimen
JP4081436B2 (ja) 2001-06-20 2008-04-23 大日本住友製薬株式会社 核酸導入を促進させる方法
JP4022614B2 (ja) * 2002-03-25 2007-12-19 国立大学法人大阪大学 新規なバイオビーズの作製方法
US6863406B2 (en) * 2002-08-01 2005-03-08 The University Of Chicago Apparatus and method for fabricating, sorting, and integrating materials with holographic optical traps
US7361171B2 (en) 2003-05-20 2008-04-22 Raydiance, Inc. Man-portable optical ablation system
US8173929B1 (en) 2003-08-11 2012-05-08 Raydiance, Inc. Methods and systems for trimming circuits
US8921733B2 (en) 2003-08-11 2014-12-30 Raydiance, Inc. Methods and systems for trimming circuits
US9022037B2 (en) 2003-08-11 2015-05-05 Raydiance, Inc. Laser ablation method and apparatus having a feedback loop and control unit
US20050095578A1 (en) * 2003-10-31 2005-05-05 Koller Manfred R. Method and apparatus for cell permeabilization
WO2005061717A1 (ja) * 2003-12-19 2005-07-07 Dainippon Sumitomo Pharma Co., Ltd. 新規な核酸導入法
US7425426B2 (en) * 2004-03-15 2008-09-16 Cyntellect, Inc. Methods for purification of cells based on product secretion
JP4504082B2 (ja) 2004-04-28 2010-07-14 富士通株式会社 液体注入装置
JP2006006223A (ja) * 2004-06-25 2006-01-12 Canon Inc 微粒子の細胞への導入と回収装置、及びその導入と細胞あるいは細胞内の標的生体分子の回収方法
US20090062184A1 (en) * 2005-03-24 2009-03-05 Dainippon Sumitomo Pharma Co., Ltd. Fine particulate preparation comprising complex of nucleic acid molecule and collagen
US8135050B1 (en) 2005-07-19 2012-03-13 Raydiance, Inc. Automated polarization correction
DK2602326T3 (da) 2005-12-13 2018-12-10 Univ Pennsylvania Fremgangsmåder til transficering af nukleinsyrer til levende celler
US9157066B2 (en) 2005-12-13 2015-10-13 The Trustees Of The University Of Pennsylvania Transcriptome transfer produces cellular phenotype conversion
US10647960B2 (en) 2005-12-13 2020-05-12 The Trustees Of The University Of Pennsylvania Transcriptome transfer produces cellular phenotype conversion
WO2007076458A1 (en) * 2005-12-21 2007-07-05 Primegen Biotech Llc Microinjector chip
US7444049B1 (en) 2006-01-23 2008-10-28 Raydiance, Inc. Pulse stretcher and compressor including a multi-pass Bragg grating
US8189971B1 (en) 2006-01-23 2012-05-29 Raydiance, Inc. Dispersion compensation in a chirped pulse amplification system
US8232687B2 (en) 2006-04-26 2012-07-31 Raydiance, Inc. Intelligent laser interlock system
US7822347B1 (en) 2006-03-28 2010-10-26 Raydiance, Inc. Active tuning of temporal dispersion in an ultrashort pulse laser system
US8420891B2 (en) 2007-06-21 2013-04-16 National University Corporation Nagoya University Method for introducing foreign substance into cell having cell wall
JP2009038988A (ja) * 2007-08-06 2009-02-26 Hiroshima Pref Gov 辛味成分の少ないアリウム属植物の栽培方法
WO2009140701A2 (en) * 2008-05-16 2009-11-19 Synthetic Genomics, Inc. Delivery into cells using ultra-short pulse lasers
MX2011001730A (es) * 2008-08-14 2011-03-24 Osiris Therapeutics Inc Composiciones purificadas de celulas madre mesenquimales y metodos para purificar composiciones de celulas madre mesenquimales.
US8125704B2 (en) 2008-08-18 2012-02-28 Raydiance, Inc. Systems and methods for controlling a pulsed laser by combining laser signals
US20100055756A1 (en) * 2008-08-28 2010-03-04 Gregory Spooner System and method for modifying biological cells using an ultra-short pulsed laser
WO2010081052A1 (en) * 2009-01-09 2010-07-15 Cyntellect, Inc. Genetic analysis of cells
US8788213B2 (en) 2009-01-12 2014-07-22 Intrexon Corporation Laser mediated sectioning and transfer of cell colonies
WO2010114901A1 (en) 2009-03-31 2010-10-07 The Board Of Trustees Of The University Of Arkansas Method of controlled drug release from a liposome carrier
US8884184B2 (en) 2010-08-12 2014-11-11 Raydiance, Inc. Polymer tubing laser micromachining
US9120181B2 (en) 2010-09-16 2015-09-01 Coherent, Inc. Singulation of layered materials using selectively variable laser output
AU2012326203B2 (en) 2011-10-17 2017-11-30 Massachusetts Institute Of Technology Intracellular delivery
EP2776013B8 (en) 2011-11-08 2023-08-30 The Board of Trustees of the University of Arkansas Methods and compositions for x-ray induced release from ph sensitive liposomes
US9485953B2 (en) 2012-07-19 2016-11-08 R.J. Reynolds Tobacco Company Method for treating tobacco plants with enzymes
JP6502940B2 (ja) 2013-08-16 2019-04-17 マサチューセッツ インスティテュート オブ テクノロジー 細胞への物質の選択的送達
US9265260B1 (en) 2014-04-07 2016-02-23 Gpd Technologies Llc Systems and methods for using light energy to facilitate penetration of substances in plants
US10278334B2 (en) 2014-04-07 2019-05-07 Premier Citrus Apz, Llc Systems and methods for delivering nucleic acids to a plant
US11178823B2 (en) 2014-04-07 2021-11-23 Premier Citrus Apz, Llc Systems and methods for using light energy to facilitate penetration of substances in plants
BR112017009050A2 (pt) 2014-10-31 2018-01-30 Massachusetts Inst Technology entrega de biomoléculas a células imunes
CN113897285A (zh) 2014-11-14 2022-01-07 麻省理工学院 化合物和组合物向细胞中的破坏和场实现的递送
US11125739B2 (en) 2015-01-12 2021-09-21 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
SG10201912910PA (en) 2015-07-09 2020-02-27 Massachusetts Inst Technology Delivery of materials to anucleate cells
EP3344575B1 (en) * 2015-09-04 2020-04-15 SQZ Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
MX2018007015A (es) * 2015-12-10 2018-11-09 Premier Citrus Apz Llc Sistemas y metodos para usar energia luminosa para facilitar la penetracion de sustancias en plantas.
US11191278B2 (en) 2016-03-25 2021-12-07 Premier Citrus Apz, Llc Systems and methods for delivering nucleic acids to a plant
CN111879434B (zh) * 2020-07-24 2022-04-08 之江实验室 一种测量生物组织或细胞温度的方法及装置
EP3971296A1 (en) * 2020-09-16 2022-03-23 FRAUNHOFER-GESELLSCHAFT zur Förderung der angewandten Forschung e.V. Methods for producing transformed plants
EP3971295A1 (en) * 2020-09-16 2022-03-23 FRAUNHOFER-GESELLSCHAFT zur Förderung der angewandten Forschung e.V. Methods for the production of genome edited plants

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US495050A (en) * 1893-04-11 Multiple-boring device
JPS6083584A (ja) * 1983-10-13 1985-05-11 Rikagaku Kenkyusho 生細胞内への物質移入法
DE3483934D1 (de) 1983-10-13 1991-02-21 Rikagaku Kenkyusho Verfahren und apparat zum einpflanzen eines fremdstoffes in lebende zelle.
JPS6083583A (ja) 1983-10-13 1985-05-11 Rikagaku Kenkyusho 生細胞レ−ザ−穿孔装置
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
GB8806643D0 (en) 1988-03-21 1988-04-20 Ici Plc Genetic manipulation
AU643563B2 (en) * 1990-11-01 1993-11-18 Sapporo Breweries Limited Method for preparing transformed plant
US6346101B1 (en) * 1993-07-19 2002-02-12 Research Foundation Of City College Of New York Photon-mediated introduction of biological materials into cells and/or cellular components
US5916788A (en) * 1995-10-12 1999-06-29 Sony Corporation Genetic recombination laser apparatus and genetic recombination method using the apparatus
JPH09163984A (ja) 1995-10-12 1997-06-24 Sony Corp 遺伝子組替え用レーザ装置およびこれを用いた遺伝子組替え方法

Also Published As

Publication number Publication date
DE60112398D1 (de) 2005-09-08
EP1225228B1 (en) 2005-08-03
EP1225228A3 (en) 2002-11-13
DE60112398T2 (de) 2005-12-29
JP2002325572A (ja) 2002-11-12
AU773342B2 (en) 2004-05-20
EP1225228A2 (en) 2002-07-24
AU9729001A (en) 2002-06-27
US20020115219A1 (en) 2002-08-22
US7132289B2 (en) 2006-11-07

Similar Documents

Publication Publication Date Title
DK1225228T3 (da) Fremgangsmåde til at före fremmed materiale ind i levende celler ved anvendelse af en lasertråle
GB2341580B (en) Method of etching transpant solid material with laser beam
DE60119899D1 (de) Stromerzeugeung unter verwendung von wiedereinstellbaren entladungsbatterien
GB2391506B (en) Method of severing an optical fibre using a laser beam
DE50115520D1 (de) Strahlformungsverfahren
NO981919L (no) FremgangsmÕte ved fremstilling av hydrogen ved bruk av et fast elektrolyttmembran
EP1310321A4 (en) LASER BEAM MACHINING PROCESS
DE69930194D1 (de) Brennstoffzellenanlage
DK1394929T3 (da) Fremgangsmåde til drift af et vindenergianlæg og vindenergianlæg
FR2778182B1 (fr) Procede de fabrication de carbonate de glycerol
DE60017238D1 (de) Bearbeitungsverfahren zur Perforierung mit einem Laserstrahl
FI20002096A0 (fi) Menetelmä sähköverkon vikaantumassa olevan tai viallisen lähdön tai haaran ilmaisemiseksi
DK94695A (da) Fremgangsmåde til generering af elektrisk energi
DK0919619T4 (da) Optimering af celler til endogen genaktivering
ITBO970169A0 (it) Metodo e macchina per la produzione di energia tramite reazioni di fus ione nucleare.
DK1263070T3 (da) Fremgangsmåde til forbedring af ydelsen af en brændstofcelle til direkte brændstoftilførsel
DK0678926T3 (da) Fremgangsmåde til fremstilling af katoder til brændselsceller
DE69924544D1 (de) Faserlaser
DE69940041D1 (de) Festelektrolytbrennstoffzelle
FR2787013B1 (fr) Prothese totale metatarso-phalangienne a coupe
FI19992005A (fi) Menetelmä ehdokassolun määrittämiseksi aktiivijoukkoon
DK0661767T3 (da) Fremgangsmåde til fremstilling af katoder til brændselsceller
AU7039000A (en) Method of manufacturing preflex beams
DE69801726D1 (de) Hochleistungslaser
DK1215960T3 (da) Fremgangsmåde til rekonstruktion af et ikke-menneskeligt pattedyrsembyron med differentierede celler