DK1200578T3 - Fremgangsmåde til RNase- og organisk oplösningsmiddel-fri plasmid-DNA-oprensning ved anvendelse af tangentialströmsfiltrering - Google Patents

Fremgangsmåde til RNase- og organisk oplösningsmiddel-fri plasmid-DNA-oprensning ved anvendelse af tangentialströmsfiltrering

Info

Publication number
DK1200578T3
DK1200578T3 DK00948876T DK00948876T DK1200578T3 DK 1200578 T3 DK1200578 T3 DK 1200578T3 DK 00948876 T DK00948876 T DK 00948876T DK 00948876 T DK00948876 T DK 00948876T DK 1200578 T3 DK1200578 T3 DK 1200578T3
Authority
DK
Denmark
Prior art keywords
plasmid dna
flow filtration
tangential flow
rnase
organic solvent
Prior art date
Application number
DK00948876T
Other languages
Danish (da)
English (en)
Inventor
Michelle D Butler
Darien L Cohen
David Kahn
Marjorie E Winkler
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of DK1200578T3 publication Critical patent/DK1200578T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/894—Manufacture, treatment, or detection of nanostructure having step or means utilizing biological growth
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/895—Manufacture, treatment, or detection of nanostructure having step or means utilizing chemical property
    • Y10S977/896—Chemical synthesis, e.g. chemical bonding or breaking
    • Y10S977/898—Enzymatic
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/908—Mechanical repair performed/surgical
    • Y10S977/91—Strengthening cell or tissue
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/908—Mechanical repair performed/surgical
    • Y10S977/912—Cancer cell repair
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/914—Protein engineering
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DK00948876T 1999-07-23 2000-07-21 Fremgangsmåde til RNase- og organisk oplösningsmiddel-fri plasmid-DNA-oprensning ved anvendelse af tangentialströmsfiltrering DK1200578T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14531699P 1999-07-23 1999-07-23
US16520699P 1999-11-12 1999-11-12
PCT/US2000/019963 WO2001007599A1 (en) 1999-07-23 2000-07-21 Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration

Publications (1)

Publication Number Publication Date
DK1200578T3 true DK1200578T3 (da) 2004-07-26

Family

ID=26842848

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00948876T DK1200578T3 (da) 1999-07-23 2000-07-21 Fremgangsmåde til RNase- og organisk oplösningsmiddel-fri plasmid-DNA-oprensning ved anvendelse af tangentialströmsfiltrering

Country Status (18)

Country Link
US (1) US6313285B1 (ko)
EP (1) EP1200578B1 (ko)
JP (1) JP2003505077A (ko)
KR (1) KR100669305B1 (ko)
CN (1) CN1378592A (ko)
AT (1) ATE264386T1 (ko)
BR (1) BR0012627A (ko)
CA (1) CA2378944A1 (ko)
DE (1) DE60009912T2 (ko)
DK (1) DK1200578T3 (ko)
ES (1) ES2218185T3 (ko)
HK (1) HK1048829A1 (ko)
HU (1) HUP0202295A3 (ko)
IL (1) IL147644A0 (ko)
NZ (1) NZ516641A (ko)
PL (1) PL364816A1 (ko)
PT (1) PT1200578E (ko)
WO (1) WO2001007599A1 (ko)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6602414B2 (en) * 2000-03-30 2003-08-05 Formulations Pro Molecule separation device and method combining multiple filtration media
AU2001294890A1 (en) * 2000-09-25 2002-04-02 Board Of Regents, The University Of Texas System Pei: dna vector formulations for in vitro and in vivo gene delivery
US6995246B1 (en) * 2000-10-19 2006-02-07 Akzo Nobel N.V. Methods for removing suspended particles from soluble protein solutions
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
JP3547715B2 (ja) * 2001-03-06 2004-07-28 榮 新垣 ベクター精製用装置及び方法
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
EP2290098A1 (en) 2002-01-28 2011-03-02 Ambion, Inc. Preparing crude biological extracts using protease, suitable for preparing cDNA
AU2003267175A1 (en) 2002-09-13 2004-04-30 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
CN1283792C (zh) 2003-11-14 2006-11-08 余伟明 生物大分子组分相分离法
US7790039B2 (en) * 2003-11-24 2010-09-07 Northwest Biotherapeutics, Inc. Tangential flow filtration devices and methods for stem cell enrichment
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
AU2006211216B2 (en) * 2005-01-31 2011-02-03 Merck Sharp & Dohme Llc Purification process for plasmid DNA
US20080102493A1 (en) * 2006-06-29 2008-05-01 Millipore Corporation Isolation of RNA and DNA from a biological sample
US7964350B1 (en) * 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
WO2008150826A1 (en) * 2007-05-31 2008-12-11 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
EP2088196A1 (en) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Methods and devices for producing biomolecules
WO2009129524A2 (en) * 2008-04-18 2009-10-22 The General Hospital Corporation High-throughput plasmid dna preparation
TWI469992B (zh) * 2008-08-28 2015-01-21 Baxter Int 濃縮剪切靈敏生物聚合物的方法
EP2367954B1 (en) 2008-12-19 2015-02-18 Life Technologies Corporation Proteinase k inhibitors, methods and compositions therefor
NZ600616A (en) 2009-12-01 2014-11-28 Shire Human Genetic Therapies Delivery of mrna for the augmentation of proteins and enzymes in human genetic diseases
JP6219811B2 (ja) 2011-04-01 2017-10-25 イー・エム・デイー・ミリポア・コーポレイシヨン ナノファイバー含有複合材構造体
KR102128248B1 (ko) 2011-06-08 2020-07-01 샤이어 휴먼 지네틱 테라피즈 인크. Mrna 전달을 위한 지질 나노입자 조성물 및 방법
US20150267192A1 (en) 2012-06-08 2015-09-24 Shire Human Genetic Therapies, Inc. Nuclease resistant polynucleotides and uses thereof
DK2968586T3 (en) 2013-03-14 2018-10-08 Translate Bio Inc CFTR MRNA COMPOSITIONS AND RELATED PROCEDURES AND APPLICATIONS
AU2014236396A1 (en) * 2013-03-14 2015-08-13 Shire Human Genetic Therapies, Inc. Methods for purification of messenger RNA
CN103525866A (zh) * 2013-03-22 2014-01-22 人福医药集团股份公司 制备质粒的方法
WO2015061500A1 (en) 2013-10-22 2015-04-30 Shire Human Genetic Therapies, Inc. Mrna therapy for argininosuccinate synthetase deficiency
MX2016005239A (es) 2013-10-22 2016-08-12 Shire Human Genetic Therapies Tratamiento con acido ribonucleico mensajero para la fenilcetonuria.
DK178664B1 (en) * 2013-10-25 2016-10-24 Amphi Consult V Martin Hesselsøe Aps A system and a method for concentrating traces of tissue from aquatic organisms in a water sample and use thereof
NZ721261A (en) 2013-12-18 2017-09-29 Asahi Chemical Ind Method for detecting coliform bacteria contained in milk
JP6571679B2 (ja) 2014-04-25 2019-09-04 トランスレイト バイオ， インコーポレイテッド メッセンジャーｒｎａの精製方法
SG11201706726TA (en) * 2015-04-17 2017-09-28 Emd Millipore Corp Method of purifying a biological materia of interest in a sample using nanofiber ultrafiltration membranes operated in tangential flow filtration mode
DK3294885T3 (da) 2015-05-08 2020-08-10 Curevac Real Estate Gmbh Fremgangsmåde til at fremstille rna
CN107873055B (zh) 2015-05-29 2021-09-17 库瑞瓦格房地产有限公司 包括至少一个切向流过滤步骤的产生和纯化rna的方法
AU2018224326B2 (en) 2017-02-27 2024-01-04 Translate Bio, Inc. Novel codon-optimized CFTR mRNA
MA49138A (fr) 2017-05-16 2020-03-25 Translate Bio Inc Traitement de la fibrose kystique par administration d'arnm à codons optimisés codant pour la cftr
AU2019325702A1 (en) 2018-08-24 2021-02-25 Translate Bio, Inc. Methods for purification of messenger RNA

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3128669A1 (de) * 1981-07-20 1983-02-03 Hoechst Ag, 6000 Frankfurt "plasmid p svh 1 und seine verwendung"
GB8600582D0 (en) 1986-01-10 1986-02-19 Ca Minister Nat Defence Purifying biological materials
AU605901B2 (en) 1987-09-11 1991-01-24 Ares Trading S.A. Purification process
US5256294A (en) 1990-09-17 1993-10-26 Genentech, Inc. Tangential flow filtration process and apparatus
WO1996002658A1 (en) * 1994-07-15 1996-02-01 Merck & Co., Inc. A method for large scale plasmid purification
US6011148A (en) 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
CA2995542A1 (en) 1997-09-05 1999-03-11 Genzyme Corporation Methods for generating high titer helper-free preparations of recombinant aav vectors
SE9703532D0 (sv) 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
WO1999063076A1 (en) 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification
US6268492B1 (en) 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells

Also Published As

Publication number Publication date
JP2003505077A (ja) 2003-02-12
ATE264386T1 (de) 2004-04-15
US6313285B1 (en) 2001-11-06
WO2001007599A1 (en) 2001-02-01
PT1200578E (pt) 2004-08-31
ES2218185T3 (es) 2004-11-16
NZ516641A (en) 2003-07-25
HK1048829A1 (zh) 2003-04-17
DE60009912D1 (de) 2004-05-19
HUP0202295A2 (en) 2002-10-28
HUP0202295A3 (en) 2005-01-28
KR100669305B1 (ko) 2007-01-17
PL364816A1 (en) 2004-12-13
DE60009912T2 (de) 2005-04-14
BR0012627A (pt) 2002-04-09
KR20020019123A (ko) 2002-03-09
IL147644A0 (en) 2002-08-14
EP1200578A1 (en) 2002-05-02
CA2378944A1 (en) 2001-02-01
CN1378592A (zh) 2002-11-06
EP1200578B1 (en) 2004-04-14

Similar Documents

Publication Publication Date Title
DK1200578T3 (da) Fremgangsmåde til RNase- og organisk oplösningsmiddel-fri plasmid-DNA-oprensning ved anvendelse af tangentialströmsfiltrering
DK0923592T3 (da) Fremgangsmåder til rensning af nukleinsyrer
SG156673A1 (en) Improved viral purification methods
ATE163043T1 (de) Reinigungsmethode und entsprechende vorrichtung
DE60102563D1 (de) Wasser- und abwasserbehandlungsverfahren zur entfernung von verunreinigungen
MXPA04007385A (es) Campana de extraccion y procedimiento para aspirar y/o purificar portadores contaminados.
AU6114199A (en) Isolation method for nucleic acid and apparatus
WO2002048385A3 (en) Compositions and methods for the release of nucleic acid molecules from solid matrices
AU2008331435B2 (en) Bisulphite treatment of RNA
MX341243B (es) Formulacion que comprende una enzima de degradacion de proteoglicano.
EP1581646A2 (en) Extraction of dna from biological samples
DE50208601D1 (de) Verfahren zur aufreinigung von bakterienzellen und zellbestandteilen
DK0894091T3 (da) Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning
ATE270251T1 (de) Methode zur reinigung von metallhaltigem abwasser
EP0431905B1 (en) Method for purifying phage DNA
CN102352351A (zh) 一种用磁性微粒从含微量核酸的样本中纯化dna的方法
EP1245674A3 (en) Nucleic acid purification
WO2003057720A3 (en) Recovery of biofilament proteins from biological fluids
AU6329099A (en) Method for purifying or isolating viral nucleic acids
AU2003288164A1 (en) Method for treating radioactive waste water
DK0839789T3 (da) Fremgangsmåde til oprensning af o-phthaldialdehyd
BR9807061A (pt) Purificação de dna em uma centrìfuga de fluxo contìnuo
WO2002057446A3 (de) Verfahren zur aufbereitung von biomasse zur herstellung von plasmid dna haltigem zelllysat
PT1102721E (pt) Processo e dispositivo para separar biomassa e agua
Riche et al. Nuclease-Free Water at Your Fingertips