WO2003057720A3 - Recovery of biofilament proteins from biological fluids - Google Patents

Recovery of biofilament proteins from biological fluids Download PDF

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Publication number
WO2003057720A3
WO2003057720A3 PCT/CA2003/000025 CA0300025W WO03057720A3 WO 2003057720 A3 WO2003057720 A3 WO 2003057720A3 CA 0300025 W CA0300025 W CA 0300025W WO 03057720 A3 WO03057720 A3 WO 03057720A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
recovery
biological fluids
biofilament
methods
Prior art date
Application number
PCT/CA2003/000025
Other languages
French (fr)
Other versions
WO2003057720A2 (en
Inventor
Costas N Karatzas
Yue Huang
Carl Turcotte
Original Assignee
Nexia Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nexia Biotech Inc filed Critical Nexia Biotech Inc
Priority to AU2003202330A priority Critical patent/AU2003202330A1/en
Priority to GB0416692A priority patent/GB2399820A/en
Publication of WO2003057720A2 publication Critical patent/WO2003057720A2/en
Publication of WO2003057720A3 publication Critical patent/WO2003057720A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

The present invention relates to methods of purifying and recovering biofilament proteins, such as spider silk proteins, from complex biological solutions, including, for example, cell culture media, plant extract, milk, urine, saliva, sweat, tears, seminal fluid, and blood. The process comprises steps of concentration and purification. Additional clarification steps and polishing procedures may also be added. The process employs precipitation, filtration, centrifugation and chromatography methods.
PCT/CA2003/000025 2002-01-11 2003-01-13 Recovery of biofilament proteins from biological fluids WO2003057720A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003202330A AU2003202330A1 (en) 2002-01-11 2003-01-13 Recovery of biofilament proteins from biological fluids
GB0416692A GB2399820A (en) 2002-01-11 2003-01-13 Recovery of biofilament proteins from biological fluids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34747102P 2002-01-11 2002-01-11
US60/347,471 2002-01-11

Publications (2)

Publication Number Publication Date
WO2003057720A2 WO2003057720A2 (en) 2003-07-17
WO2003057720A3 true WO2003057720A3 (en) 2004-03-04

Family

ID=23363832

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2003/000025 WO2003057720A2 (en) 2002-01-11 2003-01-13 Recovery of biofilament proteins from biological fluids

Country Status (3)

Country Link
AU (1) AU2003202330A1 (en)
GB (1) GB2399820A (en)
WO (1) WO2003057720A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050161058A1 (en) * 2004-01-26 2005-07-28 Israel Yerushalmy Spider silk dental floss
WO2007078239A2 (en) 2005-12-30 2007-07-12 Spiber Technologies Ab Spider silk proteins and methods for producing spider silk proteins
CA2790186C (en) 2010-03-31 2016-11-01 Amsilk Gmbh Separation of insoluble target proteins
FR2958496A1 (en) * 2010-04-09 2011-10-14 Bioprotein Technologies Sa PREPARATION OF RECOMBINANT HUMAN PHOSPHOLIPID PHOSPHOLIPID (PLTP) PLASMA PROTEIN FROM MILK OF TRANSGENIC ANIMALS.
CN102167724B (en) * 2011-01-04 2013-10-16 复旦大学 Controllable preparation method for high-concentration regenerated silk protein aqueous solution
US20190001272A1 (en) * 2015-12-18 2019-01-03 Tufts University Silk Solution Purification System, Concentrating System, and Methods Thereof
EP3263593A1 (en) 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
AU2019318216A1 (en) * 2018-08-10 2021-03-11 Bolt Threads, Inc. Composition for a molded body
CN109520785B (en) * 2018-11-09 2021-06-04 董艳 A middle section urine collection system for nephrology dept
US20220017580A1 (en) * 2018-11-28 2022-01-20 Bolt Threads, Inc. Alkaline purification of spider silk proteins
WO2022177949A1 (en) * 2021-02-16 2022-08-25 Connor Alexander Joseph Upcycling of plastic waste to recombinant silk proteins via pseudomonas bacteria

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156956A (en) * 1987-03-04 1992-10-20 Ajinomoto Co., Inc. Transgultaminase
WO1997008315A1 (en) * 1995-08-22 1997-03-06 Basel Richard M Cloning methods for high strength spider silk proteins
WO1999047661A2 (en) * 1998-03-17 1999-09-23 Nexia Biotechnologies, Inc. Production of biofilaments in transgenic animals
US5989894A (en) * 1990-04-20 1999-11-23 University Of Wyoming Isolated DNA coding for spider silk protein, a replicable vector and a transformed cell containing the DNA
DE10113781A1 (en) * 2000-06-09 2001-12-13 Ipk Inst Fuer Pflanzengenetik New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156956A (en) * 1987-03-04 1992-10-20 Ajinomoto Co., Inc. Transgultaminase
US5989894A (en) * 1990-04-20 1999-11-23 University Of Wyoming Isolated DNA coding for spider silk protein, a replicable vector and a transformed cell containing the DNA
WO1997008315A1 (en) * 1995-08-22 1997-03-06 Basel Richard M Cloning methods for high strength spider silk proteins
WO1999047661A2 (en) * 1998-03-17 1999-09-23 Nexia Biotechnologies, Inc. Production of biofilaments in transgenic animals
DE10113781A1 (en) * 2000-06-09 2001-12-13 Ipk Inst Fuer Pflanzengenetik New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LAZARIS A ET AL: "Spider silk fibers spun from soluble recombinant silk produced in mammalian cells", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 295, no. 5554, 18 January 2002 (2002-01-18), pages 472 - 476, XP002243786, ISSN: 0036-8075 *
SCHELLER JUERGEN ET AL: "Production of spider silk proteins in tobacco and potato", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 19, no. 6, June 2001 (2001-06-01), pages 573 - 577, XP002189903, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2003057720A2 (en) 2003-07-17
GB0416692D0 (en) 2004-09-01
GB2399820A (en) 2004-09-29
AU2003202330A1 (en) 2003-07-24

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