DE60009912D1 - Verfahren zur rnase- und organische lösemitteln-freier reinigung von plasmid-dns durch tangentialfluss-filtration - Google Patents

Verfahren zur rnase- und organische lösemitteln-freier reinigung von plasmid-dns durch tangentialfluss-filtration

Info

Publication number
DE60009912D1
DE60009912D1 DE60009912T DE60009912T DE60009912D1 DE 60009912 D1 DE60009912 D1 DE 60009912D1 DE 60009912 T DE60009912 T DE 60009912T DE 60009912 T DE60009912 T DE 60009912T DE 60009912 D1 DE60009912 D1 DE 60009912D1
Authority
DE
Germany
Prior art keywords
plasmid dna
flow filtration
tangential flow
rnase
organic solvent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60009912T
Other languages
English (en)
Other versions
DE60009912T2 (de
Inventor
D Butler
L Cohen
David Kahn
E Winkler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DE60009912D1 publication Critical patent/DE60009912D1/de
Application granted granted Critical
Publication of DE60009912T2 publication Critical patent/DE60009912T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/894—Manufacture, treatment, or detection of nanostructure having step or means utilizing biological growth
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/895—Manufacture, treatment, or detection of nanostructure having step or means utilizing chemical property
    • Y10S977/896—Chemical synthesis, e.g. chemical bonding or breaking
    • Y10S977/898—Enzymatic
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/908—Mechanical repair performed/surgical
    • Y10S977/91—Strengthening cell or tissue
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/908—Mechanical repair performed/surgical
    • Y10S977/912—Cancer cell repair
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/914—Protein engineering
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DE60009912T 1999-07-23 2000-07-21 Verfahren zur rnase- und organische lösemitteln-freier reinigung von plasmid-dns durch tangentialfluss-filtration Expired - Fee Related DE60009912T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US14531699P 1999-07-23 1999-07-23
US145316P 1999-07-23
US16520699P 1999-11-12 1999-11-12
US165206P 1999-11-12
PCT/US2000/019963 WO2001007599A1 (en) 1999-07-23 2000-07-21 Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration

Publications (2)

Publication Number Publication Date
DE60009912D1 true DE60009912D1 (de) 2004-05-19
DE60009912T2 DE60009912T2 (de) 2005-04-14

Family

ID=26842848

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60009912T Expired - Fee Related DE60009912T2 (de) 1999-07-23 2000-07-21 Verfahren zur rnase- und organische lösemitteln-freier reinigung von plasmid-dns durch tangentialfluss-filtration

Country Status (18)

Country Link
US (1) US6313285B1 (de)
EP (1) EP1200578B1 (de)
JP (1) JP2003505077A (de)
KR (1) KR100669305B1 (de)
CN (1) CN1378592A (de)
AT (1) ATE264386T1 (de)
BR (1) BR0012627A (de)
CA (1) CA2378944A1 (de)
DE (1) DE60009912T2 (de)
DK (1) DK1200578T3 (de)
ES (1) ES2218185T3 (de)
HK (1) HK1048829A1 (de)
HU (1) HUP0202295A3 (de)
IL (1) IL147644A0 (de)
NZ (1) NZ516641A (de)
PL (1) PL364816A1 (de)
PT (1) PT1200578E (de)
WO (1) WO2001007599A1 (de)

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US6602414B2 (en) * 2000-03-30 2003-08-05 Formulations Pro Molecule separation device and method combining multiple filtration media
WO2002024232A2 (en) * 2000-09-25 2002-03-28 Board Of Regents, The University Of Texas System Pei: dna vector formulations for in vitro and in vivo gene delivery
US6995246B1 (en) * 2000-10-19 2006-02-07 Akzo Nobel N.V. Methods for removing suspended particles from soluble protein solutions
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
JP3547715B2 (ja) * 2001-03-06 2004-07-28 榮 新垣 ベクター精製用装置及び方法
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
EP2163652B2 (de) 2002-01-28 2023-05-31 Life Technologies Corporation Herstellung biologische Rohderivaten unter Einzetsung Proteasen, geeignet für cDNA Synthese
JP2005538717A (ja) * 2002-09-13 2005-12-22 ヴァレンティス，インコーポレイテッド 核酸のプレパラティブスケールの精製のための装置および方法
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
CN1283792C (zh) 2003-11-14 2006-11-08 余伟明 生物大分子组分相分离法
US7790039B2 (en) * 2003-11-24 2010-09-07 Northwest Biotherapeutics, Inc. Tangential flow filtration devices and methods for stem cell enrichment
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
JP4971997B2 (ja) * 2005-01-31 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション プラスミドｄｎａの精製方法
US20080113357A1 (en) * 2006-06-29 2008-05-15 Millipore Corporation Filter device for the isolation of a nucleic acid
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
WO2008150826A1 (en) * 2007-05-31 2008-12-11 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
EP2088196A1 (de) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Verfahren und Vorrichtungen zur Herstellung von Biomolekülen
WO2009129524A2 (en) * 2008-04-18 2009-10-22 The General Hospital Corporation High-throughput plasmid dna preparation
TWI469992B (zh) * 2008-08-28 2015-01-21 Baxter Int 濃縮剪切靈敏生物聚合物的方法
WO2010071833A1 (en) * 2008-12-19 2010-06-24 Life Technologies Corporation Proteinase k inhibitors, methods and compositions therefor
EP3318248B1 (de) 2009-12-01 2019-04-10 Translate Bio, Inc. Ausgabe von mrna zur vermehrung von proteinen und enzymen bei humangenetischen erkrankungen
EP2694196B1 (de) 2011-04-01 2021-07-21 EMD Millipore Corporation Nanofaser mit verbundstrukturen
NZ719345A (en) 2011-06-08 2023-03-31 Translate Bio Inc Lipid nanoparticle compositions and methods for mrna delivery
EP2859102A4 (de) 2012-06-08 2016-05-11 Shire Human Genetic Therapies Nukleaseresistente polynukleotide und verwendungen davon
WO2014152966A1 (en) 2013-03-14 2014-09-25 Shire Human Genetic Therapies, Inc. Methods for purification of messenger rna
PL2968586T3 (pl) 2013-03-14 2019-01-31 Translate Bio, Inc. Kompozycje mrna cftr i związne z nimi sposoby i zastosowania
CN103525866A (zh) * 2013-03-22 2014-01-22 人福医药集团股份公司 制备质粒的方法
CN105658242A (zh) 2013-10-22 2016-06-08 夏尔人类遗传性治疗公司 用于苯丙酮尿症的mrna疗法
WO2015061500A1 (en) 2013-10-22 2015-04-30 Shire Human Genetic Therapies, Inc. Mrna therapy for argininosuccinate synthetase deficiency
DK178664B1 (en) * 2013-10-25 2016-10-24 Amphi Consult V Martin Hesselsøe Aps A system and a method for concentrating traces of tissue from aquatic organisms in a water sample and use thereof
ES2787174T3 (es) * 2013-12-18 2020-10-15 Asahi Chemical Ind Procedimiento para detectar bacterias coliformes contenidas en la leche
WO2015164773A1 (en) 2014-04-25 2015-10-29 Shire Human Genetic Therapies, Inc. Methods for purification of messenger rna
KR102206959B1 (ko) * 2015-04-17 2021-01-25 이엠디 밀리포어 코포레이션 접선방향 유동 여과 모드에서 작동되는 나노섬유 한외여과막을 사용하여 샘플에서 목적하는 생물학적 물질을 정제하는 방법
EP3294885B1 (de) 2015-05-08 2020-07-01 CureVac Real Estate GmbH Verfahren zur herstellung von rna
CN107873055B (zh) 2015-05-29 2021-09-17 库瑞瓦格房地产有限公司 包括至少一个切向流过滤步骤的产生和纯化rna的方法
MX2019010155A (es) 2017-02-27 2020-12-10 Translate Bio Inc Arnm de cftr optimizado por codón novedoso.
MA49138A (fr) 2017-05-16 2020-03-25 Translate Bio Inc Traitement de la fibrose kystique par administration d'arnm à codons optimisés codant pour la cftr
CN112930396B (zh) 2018-08-24 2024-05-24 川斯勒佰尔公司 用于纯化信使rna的方法

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Also Published As

Publication number Publication date
HUP0202295A3 (en) 2005-01-28
PL364816A1 (en) 2004-12-13
ATE264386T1 (de) 2004-04-15
IL147644A0 (en) 2002-08-14
HUP0202295A2 (en) 2002-10-28
NZ516641A (en) 2003-07-25
KR20020019123A (ko) 2002-03-09
JP2003505077A (ja) 2003-02-12
CA2378944A1 (en) 2001-02-01
WO2001007599A1 (en) 2001-02-01
ES2218185T3 (es) 2004-11-16
PT1200578E (pt) 2004-08-31
HK1048829A1 (zh) 2003-04-17
DK1200578T3 (da) 2004-07-26
KR100669305B1 (ko) 2007-01-17
BR0012627A (pt) 2002-04-09
DE60009912T2 (de) 2005-04-14
EP1200578B1 (de) 2004-04-14
US6313285B1 (en) 2001-11-06
EP1200578A1 (de) 2002-05-02
CN1378592A (zh) 2002-11-06

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