DK1141371T3 - Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller - Google Patents

Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller

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Publication number
DK1141371T3
DK1141371T3 DK99960956T DK99960956T DK1141371T3 DK 1141371 T3 DK1141371 T3 DK 1141371T3 DK 99960956 T DK99960956 T DK 99960956T DK 99960956 T DK99960956 T DK 99960956T DK 1141371 T3 DK1141371 T3 DK 1141371T3
Authority
DK
Denmark
Prior art keywords
mutant
methods
polypeptide
mutant cells
producing polypeptides
Prior art date
Application number
DK99960956T
Other languages
Danish (da)
English (en)
Inventor
Svend Kaasgaard
Henrik Moellgaard
Bjorn Eggert Christensen
Jan Lehmbeck
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1141371T3 publication Critical patent/DK1141371T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/69—Aspergillus oryzae
DK99960956T 1998-12-23 1999-12-22 Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller DK1141371T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199801726 1998-12-23
DKPA199900745 1999-05-27
PCT/DK1999/000726 WO2000039322A1 (en) 1998-12-23 1999-12-22 Methods for producing polypeptides in aspergillus mutant cells

Publications (1)

Publication Number Publication Date
DK1141371T3 true DK1141371T3 (da) 2009-02-02

Family

ID=26064565

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99960956T DK1141371T3 (da) 1998-12-23 1999-12-22 Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller

Country Status (9)

Country Link
EP (1) EP1141371B1 (zh)
JP (2) JP2002533133A (zh)
CN (1) CN100529096C (zh)
AT (1) ATE414782T1 (zh)
AU (1) AU1774000A (zh)
DE (1) DE69939947D1 (zh)
DK (1) DK1141371T3 (zh)
ES (1) ES2317706T3 (zh)
WO (1) WO2000039322A1 (zh)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070957A2 (en) * 2002-02-20 2003-08-28 Novozymes A/S Plant polypeptide production
DK1886582T3 (en) 2002-10-11 2015-04-20 Novozymes As Process for preparing a heat treated product
WO2004090549A1 (en) * 2003-04-11 2004-10-21 Novozymes A/S Method of screening for secreted glycosylated polypeptides
EP1620551B1 (en) 2003-04-28 2013-09-18 Novozymes A/S Phospholipase and method of producing it
ATE516347T1 (de) 2003-10-23 2011-07-15 Novozymes As Protease mit verbesserter stabilität in detergentien
EP1814996A2 (en) 2004-11-19 2007-08-08 Novozymes A/S Polypeptides having antimicrobial activity and polynucleotides encoding same
ES2543131T3 (es) 2004-12-22 2015-08-14 Novozymes North America, Inc. Enzimas para tratamiento de almidón
PL2410048T3 (pl) 2005-01-24 2017-02-28 Dsm Ip Assets B.V. Sposób otrzymywania związku będącego przedmiotem zainteresowania w komórce grzyba nitkowatego
EP1739428A1 (en) * 2005-07-01 2007-01-03 Institut Pasteur In vitro diagnostic method and kit for an aspergillus infection
US7858360B2 (en) 2005-10-17 2010-12-28 Novozymes A/S Use of fungal mutants for expression of antibodies
BRPI0713795B1 (pt) 2006-06-29 2018-03-20 Dsm Ip Assets B.V. Método de otimização de uma sequência de nucleotídeos codificadora que codifica uma sequência de aminoácidos predeterminada
DK2059590T3 (en) 2006-07-14 2015-02-23 Novozymes Inc Methods for preparation of secreted polypeptides having biological activity
US20110070579A1 (en) * 2007-10-19 2011-03-24 Masafumi Tokuoka Polyketide synthase-nonribosomal peptide synthetase gene
EP2358878B1 (en) 2008-11-20 2014-10-15 Novozymes Inc. Polypeptides having amylolytic enhancing activity and polynucleotides encoding same
ES2558698T3 (es) 2009-01-21 2016-02-08 Novozymes A/S Polipéptidos con actividad de esterasa y ácidos nucleicos que codifican los mismos
US8658409B2 (en) 2009-03-24 2014-02-25 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
US20120041171A1 (en) 2009-04-22 2012-02-16 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
CA2758396C (en) 2009-04-24 2018-06-12 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
EP3293264A1 (en) 2009-07-22 2018-03-14 DSM IP Assets B.V. Improved host cell for the production of a compound of interest
BR112012007390A2 (pt) 2009-09-30 2015-09-15 Novozymes As polipeptídeo isolado tendo atividade intensificadora celulolítica, polinucleotídeo isolado, métodos para produzir o polipeptídeo, para produzir um mutante de uma célula precursora, para inibir a expressão de um polipeptídeo, para produzir uma proteína, para degragar ou converter um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta transformada com um polinucleotídeo, molécula inibidora de filamento duplo, e, composição detergente
WO2011057086A1 (en) 2009-11-06 2011-05-12 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN102947442B (zh) 2009-11-06 2016-03-09 诺维信股份有限公司 用于糖化纤维素材料的组合物
EP2496692B1 (en) 2009-11-06 2016-03-16 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
WO2011098580A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
CA2789301A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
EP2585591B1 (en) 2010-06-25 2014-12-24 Novozymes A/S Polynucleotides having leader sequence function
WO2011161207A1 (en) 2010-06-25 2011-12-29 Novozymes A/S Polynucleotides having promoter activity
CN103068989B (zh) 2010-06-25 2015-09-09 诺维信公司 具有启动子活性的多核苷酸
PL2588603T3 (pl) 2010-06-29 2017-08-31 Dsm Ip Assets B.V. Polipeptyd mający lub wzmacniający aktywność degradacji materiału węglowodanowego i jego zastosowania
DK3318574T3 (da) 2010-06-29 2021-07-12 Dsm Ip Assets Bv Polypeptid med beta-glucosidase-aktivitet og anvendelser deraf
AU2011273686B2 (en) 2010-06-29 2014-07-10 Versalis S.P.A. Polypeptide having acetyl xylan esterase activity and uses thereof
EP2588601A1 (en) 2010-06-29 2013-05-08 DSM IP Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
ES2576062T3 (es) 2010-06-29 2016-07-05 Dsm Ip Assets B.V. Polipéptido que tiene actividad beta-glucosidasa y usos del mismo
BR112012033396A2 (pt) 2010-06-29 2015-06-23 Dsm Ip Assets Bv Polipetídeo tendo atividade swollenin e seus usos.
EP2588604B1 (en) 2010-06-30 2016-06-29 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
EA201300074A1 (ru) * 2010-07-01 2013-06-28 ДСМ АйПи АССЕТС Б.В. Способ получения представляющего интерес соединения
US9273335B2 (en) 2010-08-12 2016-03-01 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and a quinone compound and uses thereof
WO2012030844A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2012030811A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012062220A1 (en) 2010-11-12 2012-05-18 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
BR112013019038B1 (pt) 2011-01-26 2021-03-30 Novozymes A/S Célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo, para produzir uma proteína, para degradar um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico, e, construção de ácido nucleico ou vetor de expressão
CN110628803A (zh) 2011-01-26 2019-12-31 诺维信公司 具有纤维二糖水解酶活性的多肽及编码该多肽的多核苷酸
CN103517986B (zh) 2011-01-26 2016-12-07 诺维信公司 具有纤维二糖水解酶活性的多肽及编码该多肽的多核苷酸
MX337942B (es) 2011-01-26 2016-03-29 Novozymes As Polipeptidos que tienen actividad de endoglucanasa y polinucleotidos que codifican los mismos.
WO2012103350A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US9150842B2 (en) 2011-03-09 2015-10-06 Novozymes A/S Methods of increasing the cellulolytic enhancing activity of a polypeptide
MX2013012325A (es) 2011-04-28 2013-11-01 Novozymes Inc Polipeptidos que tienen actividad de endoglucanasa y polinucleotidos que codifican los mismos.
WO2012149344A1 (en) 2011-04-29 2012-11-01 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
CN102321700A (zh) * 2011-08-03 2012-01-18 无锡赛德生物工程有限公司 一种曲酸发酵工艺及发酵设备
BR112014004193A2 (pt) 2011-08-24 2017-03-28 Novozymes Inc célula hospedeira recombinante de trichoderma, métodos para produzir uma composição enzimática, composição enzimática, processos para degradar um material celulósico, para sintetizar um produto de fermentação e para fermentar um material celulósico
EP2773656B1 (en) 2011-10-31 2019-06-19 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP3597736A1 (en) 2011-11-21 2020-01-22 Novozymes A/S Gh61 polypeptide variants and polynucleotides encoding same
CN104245926B (zh) 2012-04-27 2021-05-07 诺维信股份有限公司 Gh61多肽变体以及编码其的多核苷酸
US9771570B2 (en) 2012-09-05 2017-09-26 Novozymes A/S Polypeptides having protease activity
US20150210991A1 (en) 2012-09-19 2015-07-30 Novozymes, Inc. Methods For Enhancing The Degradation Or Conversion Of Cellulosic Material
CN105861469B (zh) 2012-10-08 2020-04-14 诺维信公司 具有纤维素分解增强活性的多肽以及编码它们的多核苷酸
WO2014066141A2 (en) 2012-10-24 2014-05-01 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US9765373B2 (en) 2012-12-14 2017-09-19 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2964760B1 (en) 2013-03-08 2021-05-12 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
CA2910239A1 (en) 2013-05-10 2014-11-13 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
BR112017004251A2 (pt) 2014-09-05 2017-12-12 Novozymes As variantes de módulos de ligação a carboidrato e polinucleotídeos que os codificam
US10711259B2 (en) 2014-12-19 2020-07-14 Novozymes A/S Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
US10557127B2 (en) 2015-02-24 2020-02-11 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
US10519520B2 (en) 2015-05-27 2019-12-31 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN108350044B (zh) 2015-09-22 2022-05-24 诺维信公司 具有纤维二糖水解酶活性的多肽以及对其进行编码的多核苷酸
US11242507B2 (en) * 2017-06-07 2022-02-08 Ptt Global Chemical Public Company Limited Mutant strain Aspergillus aculeatus for producing cellulase and xylanase and preparation method thereof
WO2019038418A1 (en) 2017-08-25 2019-02-28 Novozymes A/S EXTRACTION OF ENZYME-ASSISTED GROSS PALM OIL
DK3775222T3 (da) 2018-03-26 2024-02-19 Novozymes As Chaperoneproteiner fra svampe
CN108823178B (zh) * 2018-06-08 2021-12-10 中国中医科学院中药研究所 大黄素糖基转移酶蛋白FtUGT73BE5及其编码基因与应用
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BR112021011384A2 (pt) 2018-12-12 2022-05-17 Novozymes As Polipeptídeo isolado, composição de enzimas, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo isolado, célula hospedeira recombinante, método de produção de um polipeptídeo, planta, parte da planta ou célula da planta transgênica, e, processos para degradação de um material celulósico ou hemicelulósico, para produção de um produto de fermentação e de fermentação de um material celulósico ou hemicelulósico
WO2020127198A1 (en) 2018-12-21 2020-06-25 Novozymes A/S Tandem protein expression
CN110218750B (zh) * 2019-04-24 2023-04-11 郑州轻工业学院 杂色曲霉菌株在发酵生产曲酸中应用
CN110108874B (zh) * 2019-04-30 2022-09-20 中国农业科学院油料作物研究所 快速检测环匹阿尼酸的胶体金免疫分析试纸条、制备及其应用
CN110106152B (zh) * 2019-04-30 2021-08-17 中国农业科学院油料作物研究所 杂交瘤细胞株ytt-2及其产生的抗环匹阿尼酸单克隆抗体
EP4004211A1 (en) 2019-07-25 2022-06-01 Novozymes A/S Filamentous fungal expression system
CN111139189B (zh) * 2020-01-14 2022-04-19 浙江工业大学 曲霉wbx-38及其在生产环匹阿尼酸中的应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0730655A1 (en) * 1993-12-01 1996-09-11 Novo Nordisk Biotech, Inc. $i(ASPERGILLUS) EXPRESSION SYSTEM
DE730656T1 (de) * 1993-12-01 1998-04-30 Novo Nordisk Biotech Inc Aspergillus poetidus exprimierungssystem
US5958727A (en) * 1996-09-13 1999-09-28 Novo Nordisk Biotech, Inc Methods for modifying the production of a polypeptide
DE69734936T2 (de) * 1996-09-19 2006-08-24 Novozymes A/S Wirtszellen und methoden für die produktion von proteinen

Also Published As

Publication number Publication date
CN100529096C (zh) 2009-08-19
AU1774000A (en) 2000-07-31
DE69939947D1 (de) 2009-01-02
ATE414782T1 (de) 2008-12-15
CN1333837A (zh) 2002-01-30
ES2317706T3 (es) 2009-04-16
WO2000039322A1 (en) 2000-07-06
EP1141371A1 (en) 2001-10-10
JP2002533133A (ja) 2002-10-08
JP5453343B2 (ja) 2014-03-26
JP2011188863A (ja) 2011-09-29
EP1141371B1 (en) 2008-11-19

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