WO2003010304A3 - Verfahren zur herstellung isolierter zellkulturen, kulturmedium zur kultivierung von zellkulturen und zellkultur - Google Patents

Verfahren zur herstellung isolierter zellkulturen, kulturmedium zur kultivierung von zellkulturen und zellkultur Download PDF

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Publication number
WO2003010304A3
WO2003010304A3 PCT/EP2002/008086 EP0208086W WO03010304A3 WO 2003010304 A3 WO2003010304 A3 WO 2003010304A3 EP 0208086 W EP0208086 W EP 0208086W WO 03010304 A3 WO03010304 A3 WO 03010304A3
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WIPO (PCT)
Prior art keywords
cell
cells
culture
culture medium
cell cultures
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PCT/EP2002/008086
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English (en)
French (fr)
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WO2003010304A2 (de
Inventor
Johannes Schwarz
Sigrid Schwarz
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Neuroprogen Gmbh Leipzig
Johannes Schwarz
Sigrid Schwarz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Neuroprogen Gmbh Leipzig, Johannes Schwarz, Sigrid Schwarz filed Critical Neuroprogen Gmbh Leipzig
Priority to CA002454552A priority Critical patent/CA2454552A1/en
Priority to US10/484,305 priority patent/US20040265996A1/en
Priority to AU2002336930A priority patent/AU2002336930A1/en
Priority to EP02772100A priority patent/EP1409651A2/de
Priority to JP2003515655A priority patent/JP2004536606A/ja
Publication of WO2003010304A2 publication Critical patent/WO2003010304A2/de
Publication of WO2003010304A3 publication Critical patent/WO2003010304A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/58—Adhesion molecules, e.g. ICAM, VCAM, CD18 (ligand), CD11 (ligand), CD49 (ligand)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/585—Integrins

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Kultivierung von Zellkulturen enthaltend eine Vielzahl von Zellen, wobei das Verfahren ein oder mehrere Verfahrensschritte aus der Gruppe Expansion der Zellkultur und Modifikation der Zellen der Zellkultur in einem Kulturmedium umfasst. Um eine schnellere und bezüglich der vorhandenen Zellen gleichmässigere Kultivierung zu erreichen, wird vorgeschlagen, eine Zellkultur zu verwenden, bei der die Zellen bei dem Verfahrensschritt zu einem wesentlichen Anteil als Einzelzellen und/oder Agglomerate mit schwachen Zell-Zell-Wechselwirkungen vorliegen, bei denen die Zell-Zell-Wechselwirkungen durch externe Einwirkung auf das Kulturmedium ohne Beschädigung des überwiegenden Anteils der Zellen unter Überführung der Agglomerate in separate Einzelzellen auftrennbar sind. Vorzugsweise erfolgt die Expansion und/oder Modifikation der Zellen unter Kulturbedingungen, die die für die Ausbildung von Zell-Zell-Adhäsionen verantwortlichen Rezeptoren der Zellen zumindest teilweise blockiert. Das Kulturmedium kann eine Ca2+-Konzentration von ≤ 0,5 mmol/l Kulturmedium und/oder Inhibitoren aufweisen, die für Zell-Zell-Wechselwirkungen eingehende Rezeptoren der Zellmembran der Zellen spezifisch sind.
PCT/EP2002/008086 2001-07-20 2002-07-19 Verfahren zur herstellung isolierter zellkulturen, kulturmedium zur kultivierung von zellkulturen und zellkultur WO2003010304A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002454552A CA2454552A1 (en) 2001-07-20 2002-07-19 Method for producing isolated cell cultures, culture medium for cultivating cell cultures and cell culture
US10/484,305 US20040265996A1 (en) 2001-07-20 2002-07-19 Method for the preparation of isolated cell cultures, culture meidum for the cultivation of cell cultures, and cell cultures
AU2002336930A AU2002336930A1 (en) 2001-07-20 2002-07-19 Method for producing isolated cell cultures, culture medium for cultivating cell cultures and cell culture
EP02772100A EP1409651A2 (de) 2001-07-20 2002-07-19 Verfahren zur herstellung isolierter zellkulturen, kulturmedium zur kultivierung von zellkulturen und zellkultur
JP2003515655A JP2004536606A (ja) 2001-07-20 2002-07-19 分離細胞培養物、細胞培養物の培養のための培地および細胞培養物の調製方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10134667.0 2001-07-20
DE10134667A DE10134667A1 (de) 2001-07-20 2001-07-20 Verfahren zur Herstellung isolierter Zellkulturen, Kulturmedium zur Kultivierung von Zellkulturen und Zellkultur

Publications (2)

Publication Number Publication Date
WO2003010304A2 WO2003010304A2 (de) 2003-02-06
WO2003010304A3 true WO2003010304A3 (de) 2003-09-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/008086 WO2003010304A2 (de) 2001-07-20 2002-07-19 Verfahren zur herstellung isolierter zellkulturen, kulturmedium zur kultivierung von zellkulturen und zellkultur

Country Status (7)

Country Link
US (1) US20040265996A1 (de)
EP (1) EP1409651A2 (de)
JP (1) JP2004536606A (de)
AU (1) AU2002336930A1 (de)
CA (1) CA2454552A1 (de)
DE (1) DE10134667A1 (de)
WO (1) WO2003010304A2 (de)

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US20040147020A1 (en) * 2002-11-01 2004-07-29 The Regents Of The University Of Colorado, A Body Corporate Dopamine neurons from human embryonic stem cells
US20080025953A1 (en) * 2006-07-25 2008-01-31 Kiminobu Sugaya Vigor Enhancement of Animals Via Administration of Stem Cells
US20090183795A1 (en) 2008-01-23 2009-07-23 Kevin John Ward Multi-Layer Papermaker's Forming Fabric With Long Machine Side MD Floats
US8993231B2 (en) * 2008-03-18 2015-03-31 Marshall University Research Corporation Methods for stem cell production and therapy
US7766053B2 (en) 2008-10-31 2010-08-03 Weavexx Corporation Multi-layer papermaker's forming fabric with alternating paired and single top CMD yarns
US8251103B2 (en) 2009-11-04 2012-08-28 Weavexx Corporation Papermaker's forming fabric with engineered drainage channels
WO2012048275A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
JP6633522B2 (ja) 2013-11-16 2020-01-22 テルモ ビーシーティー、インコーポレーテッド バイオリアクターにおける細胞増殖
WO2015148704A1 (en) 2014-03-25 2015-10-01 Terumo Bct, Inc. Passive replacement of media
JP6830059B2 (ja) 2014-09-26 2021-02-17 テルモ ビーシーティー、インコーポレーテッド スケジュール化された細胞フィーディング
CN107208064A (zh) * 2015-01-29 2017-09-26 国立大学法人东京大学 细胞的培养方法、细胞的凝集体、细胞凝集控制剂以及培养基
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
WO2017205667A1 (en) 2016-05-25 2017-11-30 Terumo Bct, Inc. Cell expansion
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
EP3565887A4 (de) * 2017-01-06 2020-12-02 The Regents of The University of California Verfahren zur erzeugung von skelettmuskelvorläuferzellen
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CN114438020A (zh) * 2022-01-08 2022-05-06 广东省疾病预防控制中心 一种多能干细胞诱导为神经祖细胞培养扩增方法及其应用

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030678A1 (en) * 1997-01-07 1998-07-16 Steindler Dennis A Isolated mammalian neural stem cells, methods of making such cells, and methods of using such cells
EP0879602A1 (de) * 1997-05-21 1998-11-25 Rijksuniversiteit te Leiden Methode zur Steigerung der Überlebung transplantierter Zellen
WO1999049014A1 (en) * 1998-03-25 1999-09-30 Cornell Research Foundation, Inc. Localization and propagation of neural and neuronal progenitor cells
EP0994185A2 (de) * 1998-10-17 2000-04-19 Tai-Wook Yoon Verfahren zur Kultivierung von Langerhansinsel-Zellen
WO2000078931A2 (de) * 1999-06-19 2000-12-28 Horst Peschel Neuronales zellmaterial, verwendung als transplantat und verfahren zu dessen herstellung
WO2001005940A2 (en) * 1999-07-16 2001-01-25 Neurotrophic Bioscience, Inc. Methods for producing and preparing cells for cell therapy
WO2001019967A1 (en) * 1999-09-17 2001-03-22 The Regents Of The University Of California Human inflammatory breast carcinoma xenograft capable of lymphovascular invasion and methods for its use
WO2001048148A1 (en) * 1999-12-28 2001-07-05 The Regents Of The University Of Michigan Process for ex vivo formation of mammalian bone and uses thereof

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JP5943533B2 (ja) * 2000-05-17 2016-07-06 アステリアス バイオセラピューティクス インコーポレイテッド 神経前駆細胞の集団

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030678A1 (en) * 1997-01-07 1998-07-16 Steindler Dennis A Isolated mammalian neural stem cells, methods of making such cells, and methods of using such cells
EP0879602A1 (de) * 1997-05-21 1998-11-25 Rijksuniversiteit te Leiden Methode zur Steigerung der Überlebung transplantierter Zellen
WO1999049014A1 (en) * 1998-03-25 1999-09-30 Cornell Research Foundation, Inc. Localization and propagation of neural and neuronal progenitor cells
EP0994185A2 (de) * 1998-10-17 2000-04-19 Tai-Wook Yoon Verfahren zur Kultivierung von Langerhansinsel-Zellen
WO2000078931A2 (de) * 1999-06-19 2000-12-28 Horst Peschel Neuronales zellmaterial, verwendung als transplantat und verfahren zu dessen herstellung
WO2001005940A2 (en) * 1999-07-16 2001-01-25 Neurotrophic Bioscience, Inc. Methods for producing and preparing cells for cell therapy
WO2001019967A1 (en) * 1999-09-17 2001-03-22 The Regents Of The University Of California Human inflammatory breast carcinoma xenograft capable of lymphovascular invasion and methods for its use
WO2001048148A1 (en) * 1999-12-28 2001-07-05 The Regents Of The University Of Michigan Process for ex vivo formation of mammalian bone and uses thereof

Non-Patent Citations (1)

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Title
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Publication number Publication date
WO2003010304A2 (de) 2003-02-06
AU2002336930A1 (en) 2003-02-17
EP1409651A2 (de) 2004-04-21
JP2004536606A (ja) 2004-12-09
CA2454552A1 (en) 2003-02-06
DE10134667A1 (de) 2003-02-06
US20040265996A1 (en) 2004-12-30

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