DK1131418T3 - Alpha-Amylase Varianter - Google Patents

Alpha-Amylase Varianter

Info

Publication number
DK1131418T3
DK1131418T3 DK99972255T DK99972255T DK1131418T3 DK 1131418 T3 DK1131418 T3 DK 1131418T3 DK 99972255 T DK99972255 T DK 99972255T DK 99972255 T DK99972255 T DK 99972255T DK 1131418 T3 DK1131418 T3 DK 1131418T3
Authority
DK
Denmark
Prior art keywords
variant
amylase
dna construct
parent
alpha
Prior art date
Application number
DK99972255T
Other languages
Danish (da)
English (en)
Inventor
Henrik Bisgard-Frantzen
Allan Svendsen
Carsten Andersen
Soren Kjaerulff
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1131418T3 publication Critical patent/DK1131418T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
DK99972255T 1998-11-16 1999-11-16 Alpha-Amylase Varianter DK1131418T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199801495 1998-11-16
US09/193,068 US6197565B1 (en) 1998-11-16 1998-11-16 α-Amylase variants
EP99972255A EP1131418B1 (en) 1998-11-16 1999-11-16 Alpha-amylase variants

Publications (1)

Publication Number Publication Date
DK1131418T3 true DK1131418T3 (da) 2006-06-26

Family

ID=26065829

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99972255T DK1131418T3 (da) 1998-11-16 1999-11-16 Alpha-Amylase Varianter

Country Status (11)

Country Link
US (1) US6197565B1 (https=)
EP (2) EP1131418B1 (https=)
JP (1) JP4550285B2 (https=)
CN (2) CN100374557C (https=)
AT (1) ATE318305T1 (https=)
AU (1) AU1151200A (https=)
CA (1) CA2350837C (https=)
DE (1) DE69930016T2 (https=)
DK (1) DK1131418T3 (https=)
ES (1) ES2259249T3 (https=)
WO (1) WO2000029560A1 (https=)

Families Citing this family (130)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6623948B1 (en) * 1999-03-31 2003-09-23 Novozymes A/S Nucleic acid sequences encoding alkaline alpha-amylases
CN100523182C (zh) * 1999-06-10 2009-08-05 花王株式会社 突变α-淀粉酶
JP5571274B2 (ja) * 2000-03-08 2014-08-13 ノボザイムス アクティーゼルスカブ 改変された特性を有する変異体
CN100491525C (zh) * 2000-07-28 2009-05-27 汉高两合股份公司 从芽孢杆菌a7-7(dsm 12368)中提取的新型淀粉分解酶以及含有该新型淀粉分解酶的洗涤剂和清洗剂
US20020155574A1 (en) * 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
EP2308980A3 (en) * 2000-08-01 2011-04-27 Novozymes A/S Alpha-amylase mutants with altered properties
EP1326965A2 (en) * 2000-10-13 2003-07-16 Novozymes A/S Alpha-amylase variant with altered properties
EP1975229A3 (en) * 2000-10-13 2009-03-18 Novozymes A/S Alpha-amylase variant with altered properties
JP4213475B2 (ja) 2001-05-14 2009-01-21 ノボザイムス アクティーゼルスカブ バシラス・ズブチリスペクチン酸リアーゼを含んでなる洗浄剤組成物
ATE449840T1 (de) 2001-05-15 2009-12-15 Novozymes As Alpha-amylasevariante mit veränderten eigenschaften
DE10138753B4 (de) * 2001-08-07 2017-07-20 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen
WO2003095638A1 (en) 2002-05-14 2003-11-20 Novozymes A/S Pectate lyase variants
DE10309803B4 (de) * 2003-03-05 2007-07-19 Henkel Kgaa α-Amylase-Varianten mit verbesserter Alkaliaktivität
US20040219649A1 (en) 2003-03-10 2004-11-04 Novozymes A/S Alcohol product processes
EP1633878A1 (en) * 2003-05-30 2006-03-15 Novozymes A/S Alcohol product processes
DE102004047777B4 (de) 2004-10-01 2018-05-09 Basf Se Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung
CA2592083C (en) * 2004-12-22 2017-02-21 Novozymes A/S Hybrid enzymes consisting of an endo-amylase first amino acid sequence and a carbohydrate-binding module as second amino acid sequence
ES2356703T3 (es) * 2004-12-22 2011-04-12 Novozymes A/S Enzimas híbridas que consisten en una primera secuencia de aminoácidos de endoamilasa y un módulo de unión de carbohidratos como segunda secuencia de aminoácidos.
MX2007007494A (es) * 2004-12-23 2007-08-15 Novozymes As Variantes de alfa-amilasa.
BRPI0722093A2 (pt) 2006-12-21 2014-04-01 Danisco Us Inc Genencor Div Composições e usos para um polipeptídeo de alfa-amilase da espécie bacillus 195
BRPI0808513A2 (pt) 2007-03-09 2014-08-19 Danisco Us Inc Genencor Div Variantes de alfa-amilase de espécies de bacillus alcalifílico, composições compreendendo variantes de alfa-amilase e métodos de uso
BRPI0809096A2 (pt) * 2007-03-23 2014-09-09 Danisco Us Inc Genecor Division Produção aumentada de amilase através da adição n-terminal à proteína amilase madura
EP2357220A1 (en) * 2010-02-10 2011-08-17 The Procter & Gamble Company Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent
EP3404087A1 (en) 2010-02-10 2018-11-21 Novozymes A/S Alpha-amylase variants with high stability in presence of a chelating agent
US9434932B2 (en) 2011-06-30 2016-09-06 Novozymes A/S Alpha-amylase variants
CN103857794B (zh) 2011-10-17 2018-03-20 诺维信公司 α‑淀粉酶变体以及编码它们的多核苷酸
EP3495479B1 (en) 2011-10-17 2025-08-06 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP4345161A3 (en) 2011-10-28 2024-06-12 Danisco Us Inc Variant maltohexaose-forming alpha-amylase variants
DK4026902T3 (da) 2012-06-08 2025-07-14 Danisco Us Inc Variante alfa-amylaser med øget aktivitet på stivelsespolymerer
ES2676895T5 (es) 2013-03-11 2022-04-27 Danisco Us Inc Variantes combinatorias de alfa-amilasa
EP3004342B1 (en) 2013-05-29 2023-01-11 Danisco US Inc. Novel metalloproteases
WO2014194034A2 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
WO2014194032A1 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
US20160108388A1 (en) 2013-05-29 2016-04-21 Danisco Us Inc. Novel metalloproteases
DK3080262T3 (da) 2013-12-13 2019-05-06 Danisco Us Inc Serinproteaser af bacillus-arter
EP3910057A1 (en) 2013-12-13 2021-11-17 Danisco US Inc. Serine proteases of the bacillus gibsonii-clade
KR20160099629A (ko) 2013-12-16 2016-08-22 이 아이 듀폰 디 네모아 앤드 캄파니 점도 조절제로서의 폴리 알파-1,3-글루칸 에테르의 사용
ES2835703T3 (es) 2013-12-18 2021-06-23 Nutrition & Biosciences Usa 4 Inc Eteres de poli alfa-1,3-glucano catiónicos
CN103834606A (zh) * 2014-01-16 2014-06-04 北京中科星冠生物技术有限责任公司 一种表达耐酸高温α-淀粉酶基因突变体的工程菌株
WO2015123323A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucans for viscosity modification
EP3116914B8 (en) 2014-03-11 2021-04-21 E. I. du Pont de Nemours and Company Oxidized poly alpha-1,3-glucan as detergent builder
US20170096653A1 (en) 2014-03-21 2017-04-06 Danisco Us Inc. Serine proteases of bacillus species
EP3129478B1 (en) 2014-04-10 2019-03-27 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3158043B1 (en) 2014-06-19 2021-03-10 Nutrition & Biosciences USA 4, Inc. Compositions containing one or more poly alpha-1,3-glucan ether compounds
DK3207129T3 (da) 2014-10-17 2020-02-24 Danisco Us Inc Serinproteaser af bacillus-arten
CN107148472A (zh) 2014-10-27 2017-09-08 丹尼斯科美国公司 芽孢杆菌属物种的丝氨酸蛋白酶
EP3212783B1 (en) 2014-10-27 2024-06-26 Danisco US Inc. Serine proteases
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
EP3957729A1 (en) 2014-10-27 2022-02-23 Danisco US Inc. Serine proteases
DK3212781T3 (da) 2014-10-27 2019-12-16 Danisco Us Inc Serinproteaser
CN108064306B (zh) 2014-12-23 2022-11-01 营养与生物科学美国4公司 酶促产生的纤维素
AU2016259703B2 (en) 2015-05-08 2021-12-23 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
WO2016180749A1 (en) 2015-05-08 2016-11-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
CN107835855B (zh) 2015-05-13 2022-05-13 丹尼斯科美国公司 AprL-进化枝蛋白酶变体及其用途
FI3307427T3 (fi) 2015-06-09 2023-11-09 Danisco Us Inc Osmoottiset puhkeavat kapselit
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
US11499146B2 (en) 2015-06-17 2022-11-15 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
EP4141113A1 (en) 2015-11-05 2023-03-01 Danisco US Inc Paenibacillus sp. mannanases
JP7364330B2 (ja) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク パエニバチルス（Ｐａｅｎｉｂａｃｉｌｌｕｓ）属種及びバチルス（Ｂａｃｉｌｌｕｓ）属種のマンナナーゼ
EP3374401B1 (en) 2015-11-13 2022-04-06 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
WO2017083226A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
JP7045313B2 (ja) 2015-11-13 2022-03-31 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
US11920170B2 (en) 2015-12-09 2024-03-05 Danisco Us Inc. Alpha-amylase combinatorial variants
EP3390625B1 (en) 2015-12-18 2023-09-06 Danisco US Inc. Polypeptides with endoglucanase activity and uses thereof
JP2019518440A (ja) 2016-05-03 2019-07-04 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
CN109072213A (zh) 2016-05-05 2018-12-21 丹尼斯科美国公司 蛋白酶变体及其用途
CN109312319B (zh) * 2016-05-09 2023-05-16 诺维信公司 具有改善的性能的变体多肽及其用途
WO2017210295A1 (en) 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
EP3472313B1 (en) 2016-06-17 2022-08-31 Danisco US Inc. Protease variants and uses thereof
US10662417B2 (en) 2016-07-05 2020-05-26 Novozymes A/S Pectate lyase variants and polynucleotides encoding same
EP3535365A2 (en) 2016-11-07 2019-09-11 Danisco US Inc. Laundry detergent composition
CN110312795B (zh) 2016-12-21 2024-07-23 丹尼斯科美国公司 蛋白酶变体及其用途
EP3559226B1 (en) 2016-12-21 2023-01-04 Danisco US Inc. Bacillus gibsonii-clade serine proteases
ES2965826T3 (es) 2017-02-01 2024-04-17 Procter & Gamble Composiciones limpiadoras que comprenden variantes de amilasa
CN107312764B (zh) * 2017-02-21 2020-05-12 南京百斯杰生物工程有限公司 α淀粉酶变体
JP7231228B2 (ja) 2017-02-24 2023-03-01 ダニスコ・ユーエス・インク バチルス・リケニフォルミスにおける増加したタンパク質産生のための組成物及び方法
US11453871B2 (en) 2017-03-15 2022-09-27 Danisco Us Inc. Trypsin-like serine proteases and uses thereof
EP3601553B1 (en) 2017-03-31 2025-12-03 Danisco US Inc. Alpha-amylase combinatorial variants
EP3601515A1 (en) 2017-03-31 2020-02-05 Danisco US Inc. Delayed release enzyme formulations for bleach-containing detergents
MX2019014556A (es) 2017-06-30 2020-02-07 Danisco Us Inc Particulas que contienen enzimas de baja aglomeracion.
EP3668973A2 (en) 2017-08-18 2020-06-24 Danisco US Inc. Alpha-amylase variants
WO2019040412A1 (en) 2017-08-23 2019-02-28 Danisco Us Inc METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS
KR20200047668A (ko) 2017-09-13 2020-05-07 다니스코 유에스 인크. 바실러스에서 증가된 단백질 생산을 위한 변형된 5'-비번역 영역(utr) 서열
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
MX2020006518A (es) 2017-12-21 2020-10-28 Danisco Us Inc Gránulos de fusión en caliente, que contienen enzimas, que comprenden un desecante termotolerante.
KR102715197B1 (ko) 2018-01-03 2024-10-08 다니스코 유에스 인크. 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법
US20200359656A1 (en) 2018-02-08 2020-11-19 Danisco Us Inc. Thermally-resistant wax matrix particles for enzyme encapsulation
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
EP3830231A1 (en) 2018-07-31 2021-06-09 Danisco US Inc. Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid
WO2020047215A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Enzyme-containing granules
WO2020068486A1 (en) 2018-09-27 2020-04-02 Danisco Us Inc Compositions for medical instrument cleaning
BR112021006967A2 (pt) 2018-10-12 2021-07-13 Danisco Us Inc. alfa-amilases com mutações que melhoram a estabilidade na presença de quelantes
CN109207458B (zh) * 2018-10-31 2021-06-25 福州大学 耐热型中温α-淀粉酶及其应用
US12509672B2 (en) 2018-11-28 2025-12-30 Danisco Us Inc. Subtilisin variants having improved stability
US20220220419A1 (en) 2019-05-24 2022-07-14 Danisco Us Inc Subtilisin variants and methods of use
US20220306968A1 (en) 2019-06-06 2022-09-29 Danisco Us Inc Methods and compositions for cleaning
EP4031560A1 (en) 2019-08-14 2022-07-27 Danisco US Inc Compositions and methods for increased protein production in bacillus licheniformis
BR112022007697A2 (pt) 2019-10-24 2022-07-12 Danisco Us Inc Alfa-amilase variante que forma maltopentaose/maltohexaose
WO2021096857A1 (en) 2019-11-11 2021-05-20 Danisco Us Inc Compositions and methods for enhanced protein production in bacillus cells
CN114945665A (zh) 2020-01-15 2022-08-26 丹尼斯科美国公司 用于增强地衣芽孢杆菌中蛋白产生的组合物和方法
EP4204553A1 (en) 2020-08-27 2023-07-05 Danisco US Inc. Enzymes and enzyme compositions for cleaning
EP4284906A1 (en) 2021-01-29 2023-12-06 Danisco US Inc. Compositions for cleaning and methods related thereto
WO2022178432A1 (en) 2021-02-22 2022-08-25 Danisco Us Inc. Methods and compositions for producing proteins of interest in pigment deficient bacillus cells
WO2022199418A1 (en) 2021-03-26 2022-09-29 Novozymes A/S Detergent composition with reduced polymer content
EP4363565A1 (en) 2021-06-30 2024-05-08 Danisco US Inc. Variant lipases and uses thereof
WO2023023644A1 (en) 2021-08-20 2023-02-23 Danisco Us Inc. Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations
US20240384205A1 (en) 2021-09-03 2024-11-21 Danisco Us Inc. Laundry compositions for cleaning
CN117957318A (zh) 2021-09-13 2024-04-30 丹尼斯科美国公司 含有生物活性物质的颗粒
EP4448747A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Variant maltopentaose/maltohexaose-forming alpha-amylases
CN118679251A (zh) 2021-12-16 2024-09-20 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
EP4448750A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and uses thereof
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
CN118974227A (zh) 2022-03-01 2024-11-15 丹尼斯科美国公司 用于清洁的酶和酶组合物
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023250301A1 (en) 2022-06-21 2023-12-28 Danisco Us Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
WO2024050339A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Mannanase variants and methods of use
EP4581119A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Detergent compositions and methods related thereto
CN120112635A (zh) 2022-09-02 2025-06-06 丹尼斯科美国公司 枯草杆菌蛋白酶变体及其相关方法
EP4615968A1 (en) 2022-11-09 2025-09-17 Danisco US Inc. Subtilisin variants and methods of use
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
CN120712348A (zh) 2023-03-06 2025-09-26 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
EP4680013A1 (en) 2023-03-16 2026-01-21 Nutrition & Biosciences USA 4, Inc. Brevibacillus fermentate extracts for cleaning and malodor control and use thereof
CN121909283A (zh) 2023-09-28 2026-04-21 丹尼斯科美国公司 具有改善的溶解度的变体角质酶及其用途
CN121969733A (zh) 2023-10-20 2026-05-01 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2026024921A1 (en) 2024-07-25 2026-01-29 The Procter & Gamble Company Detergent composition comprising a subtilisin variant and methods of use
WO2026050315A1 (en) 2024-08-29 2026-03-05 Danisco Us Inc. Subtilisin variants and methods of use

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2173329C (en) * 1993-10-08 2011-07-12 Henrik Bisgard-Frantzen Amylase variants
US5824531A (en) * 1994-03-29 1998-10-20 Novid Nordisk Alkaline bacilus amylase
KR100511499B1 (ko) * 1995-02-03 2005-12-21 노보자임스 에이/에스 소정 특성을 가지는 알파-아밀라제 돌연변이체를 디자인하는 방법
AR000862A1 (es) * 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
JPH08289788A (ja) * 1995-04-20 1996-11-05 Sanmatsu Kogyo Kk 耐酸・耐熱性α−アミラーゼ遺伝子
ES2432519T3 (es) * 1996-04-30 2013-12-04 Novozymes A/S Mutantes de alfa-amilasa
US5763385A (en) * 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
CA2274806C (en) * 1996-12-09 2011-02-01 Genencor International, Inc. H mutant alpha-amylase enzymes
US6187576B1 (en) * 1997-10-13 2001-02-13 Novo Nordisk A/S α-amylase mutants

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DE69930016T2 (de) 2006-10-05
JP4550285B2 (ja) 2010-09-22
CA2350837A1 (en) 2000-05-25
US6197565B1 (en) 2001-03-06
ES2259249T3 (es) 2006-09-16
EP1131418B1 (en) 2006-02-22
CN100374557C (zh) 2008-03-12
WO2000029560A1 (en) 2000-05-25
DE69930016D1 (de) 2006-04-27
EP1676913A3 (en) 2007-08-29
CA2350837C (en) 2011-10-18
EP1676913B1 (en) 2015-08-05
CN1333821A (zh) 2002-01-30
CN101240269A (zh) 2008-08-13
EP1676913A2 (en) 2006-07-05
AU1151200A (en) 2000-06-05
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ATE318305T1 (de) 2006-03-15

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