DK0846164T3 - Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease - Google Patents

Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease

Info

Publication number
DK0846164T3
DK0846164T3 DK96926574T DK96926574T DK0846164T3 DK 0846164 T3 DK0846164 T3 DK 0846164T3 DK 96926574 T DK96926574 T DK 96926574T DK 96926574 T DK96926574 T DK 96926574T DK 0846164 T3 DK0846164 T3 DK 0846164T3
Authority
DK
Denmark
Prior art keywords
protease
hcv
polypeptides
proteolytic activity
matter
Prior art date
Application number
DK96926574T
Other languages
Danish (da)
English (en)
Inventor
Licia Tomei
Christian Steinkuehler
Antonello Pessi
Elisabetta Bianchi
Marina Taliani
Andrea Urbani
Francesco Raffaele De
Frank Narjes
Original Assignee
Angeletti P Ist Richerche Bio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=11403536&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0846164(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Angeletti P Ist Richerche Bio filed Critical Angeletti P Ist Richerche Bio
Application granted granted Critical
Publication of DK0846164T3 publication Critical patent/DK0846164T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DK96926574T 1995-08-22 1996-08-20 Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease DK0846164T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IT95RM000573A IT1277914B1 (it) 1995-08-22 1995-08-22 Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e
EP96926574A EP0846164B1 (en) 1995-08-22 1996-08-20 Method for assaying in vitro the proteolytic activity of polypeptides having hcv ns3 protease activity and peptide substrates to be used in this method.
PCT/IT1996/000163 WO1997008304A2 (en) 1995-08-22 1996-08-20 Methodology to produce, purify and assay polypeptides with the proteolytic activity of the hcv ns3 protease

Publications (1)

Publication Number Publication Date
DK0846164T3 true DK0846164T3 (da) 2005-06-27

Family

ID=11403536

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96926574T DK0846164T3 (da) 1995-08-22 1996-08-20 Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease

Country Status (14)

Country Link
US (1) US6197536B1 (ja)
EP (1) EP0846164B1 (ja)
JP (1) JP3310298B2 (ja)
CN (1) CN1193997A (ja)
AT (1) ATE290070T1 (ja)
AU (1) AU716379B2 (ja)
BR (1) BR9610039A (ja)
CA (1) CA2228265C (ja)
DE (1) DE69634406T2 (ja)
DK (1) DK0846164T3 (ja)
ES (1) ES2236743T3 (ja)
IT (1) IT1277914B1 (ja)
PT (1) PT846164E (ja)
WO (1) WO1997008304A2 (ja)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1285158B1 (it) * 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il
GB9623908D0 (en) * 1996-11-18 1997-01-08 Hoffmann La Roche Amino acid derivatives
GB9707659D0 (en) * 1997-04-16 1997-06-04 Peptide Therapeutics Ltd Hepatitis C NS3 Protease inhibitors
ES2143918B1 (es) * 1997-05-23 2001-01-01 Inst Cientifico Tecnol Navarra Peptidos inhibidores de la proteasa c y su utilizacion en la preparacion de composiciones utiles en las infecciones causadas por el virus de la hepatitis c.
AU1416099A (en) 1997-11-28 1999-06-16 Schering Corporation Single-chain recombinant complexes of hepatitis c virus ns3 protease and ns4a cofactor peptide
IT1299134B1 (it) * 1998-02-02 2000-02-29 Angeletti P Ist Richerche Bio Procedimento per la produzione di peptidi con proprieta' inibitrici della proteasi ns3 del virus hcv, peptidi cosi' ottenibili e peptidi
AU4855299A (en) * 1998-07-02 2000-01-24 University Of Florida Ns4a-ns3 catalytic domain of hepatitis c
US6323180B1 (en) * 1998-08-10 2001-11-27 Boehringer Ingelheim (Canada) Ltd Hepatitis C inhibitor tri-peptides
WO2000040707A1 (en) 1999-01-08 2000-07-13 Bristol-Myers Squibb Co. Modified forms of hepatitis c virus ns3 protease
EP1177211B1 (en) * 1999-05-04 2008-02-20 Boehringer Ingelheim (Canada) Ltd. Surrogate cell-based system and method for assaying the activity of hepatitis c virus ns3 protease
WO2001016379A1 (en) * 1999-08-30 2001-03-08 Merck & Co., Inc. Hepatitis c virus replication inhibitors
GB0006537D0 (en) 2000-03-17 2000-05-10 Angeletti P Ist Richerche Bio Assays and screening methods
CA2430661C (en) 2000-12-15 2010-10-19 Boehringer Ingelheim (Canada) Ltd. Purified active hcv ns2/3 protease
DE60318954T2 (de) * 2002-10-29 2009-01-22 Boehringer Ingelheim International Gmbh Inhibitor-resistente hcv-ns3-protease
US7834145B2 (en) 2005-03-22 2010-11-16 Merck Sharp & Dohme Corp. HCV protease substrates
JP2009513113A (ja) * 2005-10-28 2009-04-02 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング Ｃ型肝炎ウイルスｎｓ２／３活性のアッセイ法
CN103012565B (zh) * 2013-01-15 2014-07-16 成都天台山制药有限公司 一种曲普瑞林及其固相合成制备方法
JP6564539B1 (ja) 2018-09-14 2019-08-21 長瀬産業株式会社 スルホン酸化合物によるペプチド精製方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7675491A (en) * 1990-04-04 1991-10-30 Chiron Corporation Hepatitis c virus protease
EP0729973A4 (en) * 1993-10-29 1998-12-30 Srl Inc AN ANTIQUE PEPTIDE COMPOUND AND IMMUNOASSAY PROCEDURE
JPH08205893A (ja) * 1994-12-08 1996-08-13 Sumitomo Metal Ind Ltd Ｈｃｖプロテアーゼ活性測定法
US5767233A (en) * 1995-05-12 1998-06-16 Schering Corporation Soluble cleavable substrates of the hepatitis C virus protease

Also Published As

Publication number Publication date
JP3310298B2 (ja) 2002-08-05
AU716379B2 (en) 2000-02-24
CA2228265A1 (en) 1997-03-06
CA2228265C (en) 2002-08-13
EP0846164A2 (en) 1998-06-10
ITRM950573A1 (it) 1997-02-22
ITRM950573A0 (it) 1995-08-22
US6197536B1 (en) 2001-03-06
BR9610039A (pt) 1999-07-06
DE69634406D1 (de) 2005-04-07
PT846164E (pt) 2005-07-29
EP0846164B1 (en) 2005-03-02
CN1193997A (zh) 1998-09-23
WO1997008304A3 (en) 1997-05-22
ES2236743T3 (es) 2005-07-16
WO1997008304A2 (en) 1997-03-06
ATE290070T1 (de) 2005-03-15
AU6668696A (en) 1997-03-19
IT1277914B1 (it) 1997-11-12
DE69634406T2 (de) 2006-04-13
JPH10511556A (ja) 1998-11-10

Similar Documents

Publication Publication Date Title
DK0846164T3 (da) Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease
Dang et al. Residue 225 determines the Na (+)-induced allosteric regulation of catalytic activity in serine proteases.
Smith et al. Bovine liver glutamate dehydrogenase: tentative amino acid sequence; identification of a reactive lysine; nitration of a specific tyrosine and loss of allosteric inhibition by guanosine triphosphate
Darke et al. HIV-1 protease specificity of peptide cleavage is sufficient for processing of gag and pol polyproteins
Yaron et al. Proline-dependent structural and biological properties of peptides and proteins
CA2182521A1 (en) Method for Reproducing in Vitro the Proteolytic Activity of the NS3 Protease of Hepatitis C Virus (HCV)
Stoecker et al. Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease
RU96119350A (ru) Способ репродукции in vitro протеолитической активности ns3 протеазы вируса гепатита c(нсу)
WO1990003435A3 (fr) Acide nucleique codant pour l'enzyme de conversion de l'angiotensine (eca) humaine, et ses applications, notamment pour le diagnostic in vitro de l'hypertension arterielle
Louis et al. Chemical synthesis and expression of the HIV-1 protease gene in E. coli
Bianchi et al. Inhibiting viral proteases: challenges and opportunities
Ortiz et al. Diastereotopic covalent binding of the natural inhibitor leupeptin to trypsin: detection of two interconverting hemiacetals by solution and solid-state NMR spectroscopy
DE69418035T2 (de) Hiv-proteaseinhibitoren
Smith et al. The evolution of proteinase substrates with special reference to dipeptidylpeptidase IV
Schnölzer et al. Comparative properties of feline immunodeficiency virus (FIV) and human immunodeficiency virus type 1 (HIV-1) proteinases prepared by total chemical synthesis
Suhrbier et al. BLT esterase activity as an alternative to chromium release in cytotoxic T cell assays
Kim et al. Mutational analysis of the substrate binding site of human complement factor D
Hiltkel et al. Structure/function analysis of human cystatin SN and comparison of the cysteine proteinase inhibitory profiles of human cystatins C and SN
Kim et al. Catalytic role of a surface loop of the complement serine protease factor D.
Korant Role of cellular and viral proteases in the processing of picornavirus proteins
Pessi A personal account of the role of peptide research in drug discovery: the case of hepatitis C 1
Tang et al. Understanding HIV protease: can it be translated into effective therapy against AIDS?
Matsuda et al. Proteolytic activity against model peptides of the cell wall lytic enzyme from Chlamydomonas
Turner et al. Pharmacology of enkephalinase inhibitors
Gardner et al. N-terminal fragments of CCK-(26-33) as cholecystokinin receptor antagonists in guinea pig pancreatic acini