DK0794255T3 - Fremgangsmåde til spaltning af kimært protein ved anvendelse af processeringsenzym - Google Patents

Fremgangsmåde til spaltning af kimært protein ved anvendelse af processeringsenzym

Info

Publication number
DK0794255T3
DK0794255T3 DK97301407T DK97301407T DK0794255T3 DK 0794255 T3 DK0794255 T3 DK 0794255T3 DK 97301407 T DK97301407 T DK 97301407T DK 97301407 T DK97301407 T DK 97301407T DK 0794255 T3 DK0794255 T3 DK 0794255T3
Authority
DK
Denmark
Prior art keywords
chimeric protein
processing enzyme
cleavage
peptide
arg
Prior art date
Application number
DK97301407T
Other languages
Danish (da)
English (en)
Inventor
Koji Magota
Yuji Suzuki
Toyofumi Masuda
Original Assignee
Daiichi Suntory Pharma Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daiichi Suntory Pharma Co Ltd filed Critical Daiichi Suntory Pharma Co Ltd
Application granted granted Critical
Publication of DK0794255T3 publication Critical patent/DK0794255T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
DK97301407T 1996-03-04 1997-03-04 Fremgangsmåde til spaltning af kimært protein ved anvendelse af processeringsenzym DK0794255T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP7090696 1996-03-04
EP97301407A EP0794255B1 (fr) 1996-03-04 1997-03-04 Procédé de clivage d'une protéine chimérique à l'aide d'un enzyme de traitement

Publications (1)

Publication Number Publication Date
DK0794255T3 true DK0794255T3 (da) 2005-06-27

Family

ID=13445041

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97301407T DK0794255T3 (da) 1996-03-04 1997-03-04 Fremgangsmåde til spaltning af kimært protein ved anvendelse af processeringsenzym

Country Status (11)

Country Link
US (1) US5891671A (fr)
EP (1) EP0794255B1 (fr)
JP (1) JP3925982B2 (fr)
KR (1) KR100468268B1 (fr)
CN (1) CN1216901C (fr)
AT (1) ATE290088T1 (fr)
AU (1) AU734397B2 (fr)
CA (1) CA2198966C (fr)
DE (1) DE69732583T2 (fr)
DK (1) DK0794255T3 (fr)
TW (1) TW480270B (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2284847A1 (fr) * 1998-01-30 1999-08-05 Suntory Limited Procede de production de peptides au moyen d'un peptide auxiliaire
JP4741076B2 (ja) * 1998-07-10 2011-08-03 サイオス インコーポレイテッド ８より上または５未満のｐＩを有するペプチドを産生するための方法
DK1795598T3 (da) 1999-07-23 2010-02-01 Kenji Kangawa Hidtil ukendte peptider
JP2003514532A (ja) * 1999-11-19 2003-04-22 トランスカーヨティック セラピーズ インコーポレイテッド 生成物の産生量を最適化するための核酸構築体
US6730306B1 (en) * 2000-03-08 2004-05-04 Large Scale Biology Corporation Parvovirus vaccine as viral coat protein fusions
EP1493747B1 (fr) 2002-04-11 2012-12-19 Daiichi Sankyo Company, Limited Procede de production d'un peptide modifie
GB0308732D0 (en) * 2003-04-15 2003-05-21 Axcess Ltd Absorption enhancers
GB0308734D0 (en) * 2003-04-15 2003-05-21 Axcess Ltd Uptake of macromolecules
BR112017011662A2 (pt) 2014-12-01 2018-02-27 Pfenex Inc parceiros de fusão para a produção de peptídeo
CN110305223B (zh) * 2019-06-26 2022-05-13 重庆派金生物科技有限公司 重组串联融合蛋白制备目标多肽的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60217894A (ja) * 1984-04-14 1985-10-31 Suntory Ltd 新規プロテア−ゼ及びその製造方法
RU2091490C1 (ru) * 1985-10-25 1997-09-27 Зимодженетикс, Инк. Способ получения гетерологичного полипептида в эукариотических микроорганизмов
DE3805150A1 (de) * 1987-04-11 1988-10-20 Hoechst Ag Gentechnologisches verfahren zur herstellung von polypeptiden
DE3725320A1 (de) * 1987-07-30 1989-02-09 Biotechnolog Forschung Gmbh Expressionsvektoren und verfahren unter deren verwendung zur gewinnung von cro/ss-galaktosidase/pth-fusionsproteinen und von pth
JPH07108232B2 (ja) * 1990-10-09 1995-11-22 エム・ディ・リサーチ株式会社 ペプチド又は蛋白質の製造方法
JPH05328492A (ja) * 1992-05-21 1993-12-10 Matsushita Electric Ind Co Ltd スピーカ用ダンパー
CA2198968C (fr) * 1996-03-04 2010-02-09 Toyofumi Masuda Procede de production de derives kex2 secretoires

Also Published As

Publication number Publication date
CA2198966A1 (fr) 1997-09-04
KR100468268B1 (ko) 2005-06-27
AU1503697A (en) 1997-09-11
JP3925982B2 (ja) 2007-06-06
ATE290088T1 (de) 2005-03-15
JPH09296000A (ja) 1997-11-18
US5891671A (en) 1999-04-06
EP0794255A2 (fr) 1997-09-10
EP0794255A3 (fr) 1999-01-27
AU734397B2 (en) 2001-06-14
EP0794255B1 (fr) 2005-03-02
KR970065554A (ko) 1997-10-13
CN1216901C (zh) 2005-08-31
TW480270B (en) 2002-03-21
DE69732583T2 (de) 2006-01-19
DE69732583D1 (de) 2005-04-07
CN1167155A (zh) 1997-12-10
CA2198966C (fr) 2011-06-21

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