NO20012232L - Enzymatisk amidering av peptider - Google Patents

Enzymatisk amidering av peptider

Info

Publication number
NO20012232L
NO20012232L NO20012232A NO20012232A NO20012232L NO 20012232 L NO20012232 L NO 20012232L NO 20012232 A NO20012232 A NO 20012232A NO 20012232 A NO20012232 A NO 20012232A NO 20012232 L NO20012232 L NO 20012232L
Authority
NO
Norway
Prior art keywords
terminal
polypeptide
peptides
substrate
carboxamide group
Prior art date
Application number
NO20012232A
Other languages
English (en)
Other versions
NO20012232D0 (no
Inventor
Dan Dormady
Jay S Stout
Daniel J Strydom
Barton Holmquist
Fred W Wagner
Original Assignee
Bionebraska Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bionebraska Inc filed Critical Bionebraska Inc
Publication of NO20012232D0 publication Critical patent/NO20012232D0/no
Publication of NO20012232L publication Critical patent/NO20012232L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Foreliggende oppfinnelse tilveiebringer en fremgangsmåte for å fremstille et polypeptid som har en C-terminal a-karboksamidgruppe. Spesielt omhandlet er en enzymatisk modifisering av valgte substratpolypeptider som resulterer i kløyving av substrarpolypeptidet for å danne et produktpeptid med en C-terminal argininrest, som har en a- karboksamidgruppe (C-terminal «Arg-NH2»). Fremgangsmåten inkluderer å kontakte en vannbasert løsning som inkluderer (i) ammoniakkreagens og (ii) substratpolypeptidet med (iii) klostripain.
NO20012232A 1998-11-06 2001-05-04 Enzymatisk amidering av peptider NO20012232L (no)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10731198P 1998-11-06 1998-11-06
US09/212,663 US6461834B1 (en) 1998-11-06 1998-12-16 Clostripain catalyzed amidation of peptides
PCT/US1999/026060 WO2000028067A1 (en) 1998-11-06 1999-11-05 Enzymatic amidation of peptides

Publications (2)

Publication Number Publication Date
NO20012232D0 NO20012232D0 (no) 2001-05-04
NO20012232L true NO20012232L (no) 2001-05-04

Family

ID=26804650

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20012232A NO20012232L (no) 1998-11-06 2001-05-04 Enzymatisk amidering av peptider

Country Status (13)

Country Link
US (1) US6461834B1 (no)
EP (1) EP1127155A1 (no)
JP (1) JP2002529101A (no)
KR (1) KR20010099668A (no)
CN (1) CN1325455A (no)
AU (1) AU755130B2 (no)
CA (1) CA2349527A1 (no)
CZ (1) CZ20011523A3 (no)
HU (1) HUP0104178A3 (no)
IS (1) IS5932A (no)
MX (1) MXPA01004526A (no)
NO (1) NO20012232L (no)
WO (1) WO2000028067A1 (no)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1158304C (zh) * 2001-05-10 2004-07-21 上海华谊生物技术有限公司 蛙皮抗菌肽衍生物
HUP0700151A2 (en) * 2001-10-18 2007-05-29 Bristol Myers Squibb Co Human glucagon-like-peptide-1 mimics and their use in the treatment of diabetes and related conditions
US7238671B2 (en) 2001-10-18 2007-07-03 Bristol-Myers Squibb Company Human glucagon-like-peptide-1 mimics and their use in the treatment of diabetes and related conditions
WO2003099847A2 (en) * 2002-05-24 2003-12-04 Restoragen, Inc. Method for enzymatic production of glp-1 (7-36) amide peptides
EP1513945A4 (en) * 2002-05-24 2008-12-24 Restoragen Inc PROCESS FOR UNIVERSAL ENZYMATIC PRODUCTION OF BIOACTIVE PEPTIDES
WO2003099854A2 (en) * 2002-05-24 2003-12-04 Nps Allelix Corp. Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides
SI1704234T1 (sl) * 2003-11-21 2012-08-31 Nps Pharma Inc Proizvodnja glukagonu podobnih peptidov 2 in analogov
DE102004058306A1 (de) 2004-12-01 2006-07-27 Sanofi-Aventis Deutschland Gmbh Verfahren zur Herstellung von Carboxy-terminal amidierten Peptiden
JP5134540B2 (ja) * 2005-09-19 2013-01-30 アラーガン、インコーポレイテッド クロストリジウム毒素活性化クロストリジウム毒素

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4568640A (en) 1981-05-11 1986-02-04 Harvey Rubin Method of inserting amino acid analogs into proteins
GB8314362D0 (en) 1983-05-24 1983-06-29 Celltech Ltd Polypeptide and protein products
JPS6229997A (ja) 1985-04-08 1987-02-07 Sankyo Co Ltd Ｃ末端にプロリンアミドを有するペプチドの製法
US5416007A (en) 1987-03-20 1995-05-16 Creative Biomolecules, Inc. Enhanced proteolytic cleavage of recombinant fusion proteins
DK15888D0 (da) 1988-01-14 1988-01-14 Carlsberg Biotechnology Ltd Enzymatisk fremgangsmaade til fremstilling af immunmodulerende pentapeptider samt mellemprodukter til brug ved fremgangsmaaden
GB8829432D0 (en) 1988-12-15 1989-02-01 Celltech Ltd Enzymatic process
US5252464A (en) 1989-03-14 1993-10-12 Carlsberg Biotechnology Ltd. A/S Process for producing pentapeptides and intermediates for use in the synthesis
DE3918125A1 (de) * 1989-06-03 1990-12-06 Forschungszentrum Juelich Gmbh Verfahren zur modifizierung von proteinen und danach erhaeltliche modifizierte proteine
ES2113879T3 (es) 1990-01-24 1998-05-16 Douglas I Buckley Analogos de glp-1 utiles para el tratamiento de diabetes.
US5595887A (en) 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
US5202239A (en) 1990-08-07 1993-04-13 Scios Nova Inc. Expression of recombinant polypeptides with improved purification
NZ239652A (en) * 1990-09-05 1992-09-25 Hoechst Ag Hydrolysis of preproinsulin to insulin and analogues using clostripain and optionally carboxypeptidase
EP0518088A2 (de) * 1991-05-23 1992-12-16 Hoechst Aktiengesellschaft Verfahren zur Clostripain-katalysierten Verknüpfung von Arg-Pro und Arg-B (B=proteinogene und nicht-proteinogene Aminosäuren) enthaltenden Peptiden
AT396937B (de) 1992-04-06 1993-12-27 Immuno Ag Verfahren zur aktivierung von blutgerinnungsfaktoren
DE69332377T2 (de) 1992-07-13 2003-07-03 Bionebraska, Inc. Verfahren zur modifizierung rekombinanter polypeptide
US5332503A (en) 1993-04-16 1994-07-26 Baxter International Inc. Process for purifying collagenase
US6187579B1 (en) 1993-10-28 2001-02-13 Carlsberg A/S Customized proteases
DK144093D0 (no) 1993-12-23 1993-12-23 Novo Nordisk As
WO1996017941A2 (en) * 1994-12-07 1996-06-13 Bionebraska, Inc. Production of c-terminal amidated peptides from recombinant protein constructs

Also Published As

Publication number Publication date
AU755130B2 (en) 2002-12-05
IS5932A (is) 2001-05-02
WO2000028067A1 (en) 2000-05-18
EP1127155A1 (en) 2001-08-29
JP2002529101A (ja) 2002-09-10
MXPA01004526A (es) 2002-09-18
NO20012232D0 (no) 2001-05-04
HUP0104178A2 (hu) 2002-02-28
HUP0104178A3 (en) 2003-01-28
KR20010099668A (ko) 2001-11-09
CZ20011523A3 (cs) 2002-02-13
AU1342300A (en) 2000-05-29
CN1325455A (zh) 2001-12-05
CA2349527A1 (en) 2000-05-18
WO2000028067A9 (en) 2000-10-19
US6461834B1 (en) 2002-10-08

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Legal Events

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FC2A Withdrawal, rejection or dismissal of laid open patent application