DK0542863T3 - Ekspression af rekombinante polypeptider med forbedret oprensning - Google Patents

Ekspression af rekombinante polypeptider med forbedret oprensning

Info

Publication number
DK0542863T3
DK0542863T3 DK91915080T DK91915080T DK0542863T3 DK 0542863 T3 DK0542863 T3 DK 0542863T3 DK 91915080 T DK91915080 T DK 91915080T DK 91915080 T DK91915080 T DK 91915080T DK 0542863 T3 DK0542863 T3 DK 0542863T3
Authority
DK
Denmark
Prior art keywords
expression
recombinant polypeptides
improved purification
carrier
fusion protein
Prior art date
Application number
DK91915080T
Other languages
Danish (da)
English (en)
Inventor
S Joseph Tarnowski
Sandra Hilliker
W Scott Willett
Original Assignee
Scios Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scios Inc filed Critical Scios Inc
Application granted granted Critical
Publication of DK0542863T3 publication Critical patent/DK0542863T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
DK91915080T 1990-08-07 1991-08-07 Ekspression af rekombinante polypeptider med forbedret oprensning DK0542863T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/564,259 US5202239A (en) 1990-08-07 1990-08-07 Expression of recombinant polypeptides with improved purification
PCT/US1991/005617 WO1992002550A1 (en) 1990-08-07 1991-08-07 Expression of recombinant polypeptides with improved purification

Publications (1)

Publication Number Publication Date
DK0542863T3 true DK0542863T3 (da) 1998-08-10

Family

ID=24253763

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91915080T DK0542863T3 (da) 1990-08-07 1991-08-07 Ekspression af rekombinante polypeptider med forbedret oprensning

Country Status (10)

Country Link
US (2) US5202239A (de)
EP (1) EP0542863B1 (de)
JP (1) JP3369168B2 (de)
AT (1) ATE160587T1 (de)
CA (1) CA2089094C (de)
DE (1) DE69128285T2 (de)
DK (1) DK0542863T3 (de)
ES (1) ES2114539T3 (de)
GR (1) GR3026230T3 (de)
WO (1) WO1992002550A1 (de)

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FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
AU6360394A (en) * 1993-03-03 1994-09-26 Mayo Foundation For Medical Education And Research Vasonatrin peptide and analogs thereof
DK144093D0 (de) * 1993-12-23 1993-12-23 Novo Nordisk As
US6069230A (en) * 1994-11-10 2000-05-30 Promega Corporation High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications
US6764689B1 (en) 1994-11-10 2004-07-20 Promega Corporation High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications
US5935824A (en) * 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
US6277819B1 (en) 1996-08-30 2001-08-21 Eli Lilly And Company Use of GLP-1 or analogs in treatment of myocardial infarction
WO1998024886A1 (en) * 1996-12-04 1998-06-11 Brigham And Women's Hospital, Inc. Mast cell protease that cleaves fibrinogen
WO1998033812A1 (en) * 1997-02-05 1998-08-06 Brigham And Women's Hospital, Inc. Mast cell protease peptide inhibitors
US6077689A (en) * 1997-12-24 2000-06-20 Amgen Inc. Enhanced solubility of recombinant proteins
EP1090132A1 (de) * 1998-06-26 2001-04-11 PPL Therapeutics (Scotland) Limited Verfahren zur herstellung von rekombinanten polypetide
CA2334160C (en) * 1998-07-10 2016-09-27 N. Stephen Pollitt Method for producing a peptide with a pi above 8 or below 5
US6461834B1 (en) 1998-11-06 2002-10-08 Bionebraska, Inc. Clostripain catalyzed amidation of peptides
US6082594A (en) * 1998-12-31 2000-07-04 Snowmaker Productions, Inc. Snowfall simulator with agitating dispenser
WO2000071576A2 (en) * 1999-05-24 2000-11-30 Mayo Foundation For Medical Education And Research Adenovirus vectors encoding brain natriuretic peptide
US20060160740A1 (en) * 1999-10-21 2006-07-20 Suad Efendic Use of GLP-1 or analogs in treatment of stroke
US6407211B1 (en) * 1999-12-17 2002-06-18 Mayo Foundation For Medical Education And Research Chimeric natriuretic peptides
CA2405709A1 (en) * 2000-04-12 2001-10-25 Human Genome Sciences, Inc. Albumin fusion proteins
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US6469145B1 (en) * 2000-06-26 2002-10-22 The United States Of America As Represented By The Secretary Of The Army One-step purification process for organophosphorus hydrolase enzyme
US6632638B1 (en) 2000-11-17 2003-10-14 Amgen, Inc. Enhanced solubility of recombinant proteins using Uracil DNA glycosylase inhibitor
US20030143191A1 (en) * 2001-05-25 2003-07-31 Adam Bell Chemokine beta-1 fusion proteins
JP4088584B2 (ja) * 2001-07-26 2008-05-21 アドバンスド プロテイン テクノロジーズ コーポレイション 融合タンパク質から目的タンパク質を分離する方法。
CA2471363C (en) * 2001-12-21 2014-02-11 Human Genome Sciences, Inc. Albumin fusion proteins
AU2002364587A1 (en) * 2001-12-21 2003-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
US20030134352A1 (en) * 2002-01-04 2003-07-17 Freimuth Paul I. Facilitating protein folding and solubility by use of peptide extensions
US7229554B2 (en) * 2002-09-25 2007-06-12 Novo Nordisk A/S Purification process comprising microfiltration at elevated temperatures
EP1569683B1 (de) 2002-11-26 2010-03-03 Biocon Limited Modifizierte natriuretic verbindungen, konjugate und ihre verwendungen
CA2513213C (en) 2003-01-22 2013-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
MXPA06009072A (es) * 2004-02-09 2007-03-29 Human Genome Sciences Inc Proteinas de fusion de albumina.
US8137929B2 (en) 2005-04-15 2012-03-20 Novo Nordisk Health Care Ag Basic protein purification tags from thermophilic bacteria
US7334279B2 (en) * 2005-11-09 2008-02-26 Oprandi Arthur V Mattress pad
US8580746B2 (en) 2006-03-30 2013-11-12 Palatin Technologies, Inc. Amide linkage cyclic natriuretic peptide constructs
DK2001518T3 (da) 2006-03-30 2013-10-07 Palatin Technologies Inc Cykliske natriuretiske peptidfremstillinger
US7795221B2 (en) 2006-03-30 2010-09-14 Palatin Technologies, Inc. Linear natriuretic peptide constructs
WO2008043847A1 (en) * 2006-10-13 2008-04-17 Novo Nordisk Health Care Ag Processing enzymes fused to basic protein tags
EP2678002A2 (de) 2011-02-25 2014-01-01 Medtronic, Inc. Therapie für nierenerkrankungen und/oder herzinsuffizienz
CN103906761B (zh) 2011-08-30 2016-12-21 梅约医学教育与研究基金会 利钠多肽
US20130244937A1 (en) 2011-09-02 2013-09-19 Nile Therapeutics, Inc. Chimeric natriuretic peptide compositions and methods of preparation
US9611305B2 (en) 2012-01-06 2017-04-04 Mayo Foundation For Medical Education And Research Treating cardiovascular or renal diseases
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene

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Publication number Priority date Publication date Assignee Title
US4431739A (en) * 1979-11-05 1984-02-14 Genentech, Inc. Transformant bacterial culture capable of expressing heterologous protein
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
US4880911A (en) * 1982-03-19 1989-11-14 G. D. Searle & Co. Fused polypeptides and methods for their detection
US4897348A (en) * 1983-08-25 1990-01-30 Sri International Recombinant materials and methods for producing human connective tissue-activating peptide-III and analogs thereof
US4618600A (en) * 1984-04-19 1986-10-21 Biotechnology Research Associates, J.V. Novel polypeptide diuretic/vasodilators
CA1213537A (en) * 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
EP0207044A1 (de) * 1985-06-20 1986-12-30 Monsanto Company Freisetzung von Peptiden aus Polypeptiden
GB8518753D0 (en) * 1985-07-24 1985-08-29 Glaxo Group Ltd Microbiological products
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
US4987070A (en) * 1987-03-04 1991-01-22 Suntory Limited Use of a 97 amino acid leader sequence from the E. coli B-galactosidase gene for the production of hanp and hptc as fusion proteins

Also Published As

Publication number Publication date
ATE160587T1 (de) 1997-12-15
GR3026230T3 (en) 1998-05-29
DE69128285T2 (de) 1998-06-25
ES2114539T3 (es) 1998-06-01
CA2089094C (en) 2002-02-05
WO1992002550A1 (en) 1992-02-20
EP0542863A1 (de) 1993-05-26
US5202239A (en) 1993-04-13
DE69128285D1 (de) 1998-01-08
JP3369168B2 (ja) 2003-01-20
EP0542863B1 (de) 1997-11-26
JPH06504036A (ja) 1994-05-12
CA2089094A1 (en) 1992-02-08
US5322930A (en) 1994-06-21
EP0542863A4 (en) 1993-09-22

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