PT1095141E - Metodo para a producao de um peptido com um pi acima de 8 ou abaixo de 5 - Google Patents

Metodo para a producao de um peptido com um pi acima de 8 ou abaixo de 5

Info

Publication number
PT1095141E
PT1095141E PT99932242T PT99932242T PT1095141E PT 1095141 E PT1095141 E PT 1095141E PT 99932242 T PT99932242 T PT 99932242T PT 99932242 T PT99932242 T PT 99932242T PT 1095141 E PT1095141 E PT 1095141E
Authority
PT
Portugal
Prior art keywords
peptide
acid cleavage
fusion partner
producing
fusion
Prior art date
Application number
PT99932242T
Other languages
English (en)
Inventor
Pollitt N Stephen
Douglas I Buckley
Peter A Stathis
Taymar E Hartman
Zhong Ziyang
Original Assignee
Scios Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scios Inc filed Critical Scios Inc
Publication of PT1095141E publication Critical patent/PT1095141E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/122—Hydrolysis with acids different from HF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01028—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Cardiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Small-Scale Networks (AREA)
PT99932242T 1998-07-10 1999-07-08 Metodo para a producao de um peptido com um pi acima de 8 ou abaixo de 5 PT1095141E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US9242398P 1998-07-10 1998-07-10

Publications (1)

Publication Number Publication Date
PT1095141E true PT1095141E (pt) 2006-07-31

Family

ID=22233139

Family Applications (2)

Application Number Title Priority Date Filing Date
PT99932242T PT1095141E (pt) 1998-07-10 1999-07-08 Metodo para a producao de um peptido com um pi acima de 8 ou abaixo de 5
PT06008034T PT1714972E (pt) 1998-07-10 1999-07-08 Método para a produção de um péptido com um pi acima de 8 ou abaixo de 5

Family Applications After (1)

Application Number Title Priority Date Filing Date
PT06008034T PT1714972E (pt) 1998-07-10 1999-07-08 Método para a produção de um péptido com um pi acima de 8 ou abaixo de 5

Country Status (14)

Country Link
US (1) US6303340B1 (pt)
EP (3) EP1714972B1 (pt)
JP (2) JP4741076B2 (pt)
CN (2) CN1242062C (pt)
AT (2) ATE529515T1 (pt)
AU (1) AU4859599A (pt)
CA (1) CA2334160C (pt)
CY (2) CY1106110T1 (pt)
DE (1) DE69930938T2 (pt)
DK (2) DK1095141T3 (pt)
ES (2) ES2263278T3 (pt)
HK (2) HK1036637A1 (pt)
PT (2) PT1095141E (pt)
WO (1) WO2000003011A2 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6785496A (en) * 1995-09-07 1997-03-27 Novo Nordisk A/S Phage display for detergent enzyme activity
JP4267444B2 (ja) 2001-06-15 2009-05-27 エフ．ホフマン−ラ ロシュ アーゲー Ｇｐ４１フラグメントのアセチル化
EP1417237B1 (en) * 2001-07-26 2011-04-06 Advanced Protein Technologies Corp. Process for preparation of polypeptides of interest from fusion peolypeptides
EP2163558A3 (en) * 2004-10-04 2010-10-27 Novetide, Ltd. A Counterion Exchange Process for Peptides
JP2008054673A (ja) * 2006-08-03 2008-03-13 Hokkaido Univ 組み換え蛋白質の製造方法
US8951185B2 (en) 2007-10-26 2015-02-10 Ams Research Corporation Surgical articles and methods for treating pelvic conditions
US20090035815A1 (en) * 2007-06-29 2009-02-05 Laxmi Srinivas Rao Synthetic Gene for Enhanced Expression in E. Coli
US8609621B2 (en) 2010-11-15 2013-12-17 E I Du Pont De Nemours And Company Acid-cleavable linkers exhibiting altered rates of acid hydrolysis
KR20140148420A (ko) * 2012-03-19 2014-12-31 마들렌 파마수티컬스 피티와이 리미티드 재조합 펩티드의 생산 방법
US20150099860A1 (en) * 2013-10-08 2015-04-09 Proteins Easy Corp Methods for separating and purifying endogenous, exogenous and recombinant proteins/peptides from plants and animals using aqueous-free, anhydrous strategies
CN106928316B (zh) * 2015-12-31 2020-11-20 深圳翰宇药业股份有限公司 一种纯化氧化含二硫键多肽的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8314362D0 (en) * 1983-05-24 1983-06-29 Celltech Ltd Polypeptide and protein products
DK175535B1 (da) * 1987-03-04 2004-11-29 Daiichi Suntory Pharma Co Ltd Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid
US5114923A (en) * 1988-05-31 1992-05-19 California Biotechnology Inc. Recombinant techniques for production of novel natriuretic and vasodilator peptides
WO1990001540A1 (en) * 1988-08-11 1990-02-22 California Biotechnology Inc. Method for stabilizing heterologous protein expression and vectors for use therein
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
US5202239A (en) * 1990-08-07 1993-04-13 Scios Nova Inc. Expression of recombinant polypeptides with improved purification
JPH06508985A (ja) * 1991-02-27 1994-10-13 クリエイティブ　バイオモレキュルズ　インコーポレイテッド 富セリンペプチドリンカー
ATE253639T1 (de) * 1991-08-19 2003-11-15 Daiichi Suntory Pharma Co Ltd Verfahren zur herstellung von peptiden
JP3531947B2 (ja) * 1991-08-19 2004-05-31 第一サントリーファーマ株式会社 ペプチドの製造方法
US5589364A (en) 1994-07-29 1996-12-31 Magainin Pharmaceuticals Inc. Recombinant production of biologically active peptides and proteins
JP3995279B2 (ja) * 1994-09-07 2007-10-24 アスビオファーマ株式会社 蛋白の製造方法
CA2198966C (en) * 1996-03-04 2011-06-21 Yuji Suzuki Method for cleaving chimeric protein using processing enzyme
US5851802A (en) * 1996-03-22 1998-12-22 Xoma Corporation Methods for recombinant microbial production of fusion proteins and BPI-derived peptides

Also Published As

Publication number Publication date
EP1095141B1 (en) 2006-04-19
DK1095141T3 (da) 2006-07-31
JP2002520018A (ja) 2002-07-09
ES2374829T3 (es) 2012-02-22
CY1112195T1 (el) 2015-12-09
DE69930938D1 (de) 2006-05-24
DE69930938T2 (de) 2007-01-11
EP1714972A2 (en) 2006-10-25
WO2000003011A2 (en) 2000-01-20
EP2305815A1 (en) 2011-04-06
CN1313896A (zh) 2001-09-19
EP1714972A3 (en) 2008-05-28
HK1097280A1 (en) 2007-06-22
JP2011172575A (ja) 2011-09-08
EP2305815B1 (en) 2014-11-05
EP1095141A2 (en) 2001-05-02
CY1106110T1 (el) 2011-06-08
JP4741076B2 (ja) 2011-08-03
CA2334160C (en) 2016-09-27
DK1714972T3 (da) 2012-01-23
CA2334160A1 (en) 2000-01-20
EP1714972B1 (en) 2011-10-19
JP5291137B2 (ja) 2013-09-18
AU4859599A (en) 2000-02-01
PT1714972E (pt) 2011-12-26
ATE323765T1 (de) 2006-05-15
WO2000003011A3 (en) 2000-06-15
ATE529515T1 (de) 2011-11-15
HK1036637A1 (en) 2002-01-11
CN1170932C (zh) 2004-10-13
CN1521261A (zh) 2004-08-18
ES2263278T3 (es) 2006-12-01
CN1242062C (zh) 2006-02-15
US6303340B1 (en) 2001-10-16

Similar Documents

Publication Publication Date Title
HK1097280A1 (en) Method for producing a peptide with a pl above 8 or below 5
WO1996017924A3 (en) Novel hedgehog-derived polypeptides
WO2004003148A3 (en) Genes encoding proteins with pesticidal activity
DE69434166D1 (de) Menschliche metabotropische glutamatrezeptor untertype hmglur7 und verwandte dns-verbindungen
BR9713463A (pt) Fitase de bacillus subtilis, gene codificando a referida fitase, método para a produção e uso da mesma
BR0114475A (pt) Célula para a produção de composição de anticorpo
EP1717315A3 (en) Osteoprotegerin binding proteins and receptors
ATE263836T1 (de) Chimere proteine, welche borrelia polypeptide beinhalten, verwendungen dafür
BR0007035A (pt) Métodos de controle de pragas de agrótis
TR199802394A3 (tr) Proteinleri stabilize etmek için gelistirilmis proses.
BRPI0411843A (pt) promotores rps21 e beta-actina e usos destes
BR9909639A (pt) Processos para reduzir a alergenicidade de uma proteìna não humana e para produzir uma proteìna tendo reduzida alergenicidade, proteìna mutante tendo reduzida alergenicidade, dna, vetor de expressão, células hospedeiras, composição farmacêutica, e, substilisina humana
WO1997020045A3 (en) P2u2 purinergic receptor
CA2296560A1 (en) Amino acid derivatives useful to treat stroke
Čechová et al. Boar proacrosin is a single‐chain molecule which has the N‐terminus of the acrosin A‐chain (light chain)
CA2286296A1 (en) Inhibitors for urokinase receptor
WO1999025829A3 (en) Cdk2 protein and cdk2 protein complexes
SE9602822D0 (sv) New receptor
ES2097368T3 (es) Nuevas proteinas inhibidoras de trombina a partir de sanguijuelas terrestres.
NO940090D0 (no) Blanding for makrodefekt-fri sement, samt fremgangsmaate for frems tilling av denne
WO2003082206A3 (en) Multiple sclerosis-related immunoglobulin derived proteins, compositions, methods and uses
WO2001053339A3 (en) Egg specific surface proteins
AR034853A1 (es) Secuencia de acido nucleico sintetico que codifica una alfa-galactosidasa, vector y celula que la comprenden, metodo para producir alfa-galactosidasa, metodo para prepararla, composicion farmaceutica que comprende dicha secuencia y su uso para preparar esta ultima
SI1401871T1 (pt)
SE9604439D0 (sv) New receptor