ATE323765T1 - Verfahren zur herstellung eines peptids mit einem pi grösser als 8 oder kleiner als 5 - Google Patents

Verfahren zur herstellung eines peptids mit einem pi grösser als 8 oder kleiner als 5

Info

Publication number
ATE323765T1
ATE323765T1 AT99932242T AT99932242T ATE323765T1 AT E323765 T1 ATE323765 T1 AT E323765T1 AT 99932242 T AT99932242 T AT 99932242T AT 99932242 T AT99932242 T AT 99932242T AT E323765 T1 ATE323765 T1 AT E323765T1
Authority
AT
Austria
Prior art keywords
peptide
producing
acid cleavage
fusion partner
smaller
Prior art date
Application number
AT99932242T
Other languages
English (en)
Inventor
N Stephen Pollitt
Douglas I Buckley
Peter A Stathis
Taymar E Hartman
Ziyang Zhong
Original Assignee
Scios Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scios Inc filed Critical Scios Inc
Application granted granted Critical
Publication of ATE323765T1 publication Critical patent/ATE323765T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/122—Hydrolysis with acids different from HF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01028—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
AT99932242T 1998-07-10 1999-07-08 Verfahren zur herstellung eines peptids mit einem pi grösser als 8 oder kleiner als 5 ATE323765T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US9242398P 1998-07-10 1998-07-10

Publications (1)

Publication Number Publication Date
ATE323765T1 true ATE323765T1 (de) 2006-05-15

Family

ID=22233139

Family Applications (2)

Application Number Title Priority Date Filing Date
AT06008034T ATE529515T1 (de) 1998-07-10 1999-07-08 Verfahren zur herstellung eines peptids mit einem pi grösser als 8 oder kleiner als 5
AT99932242T ATE323765T1 (de) 1998-07-10 1999-07-08 Verfahren zur herstellung eines peptids mit einem pi grösser als 8 oder kleiner als 5

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT06008034T ATE529515T1 (de) 1998-07-10 1999-07-08 Verfahren zur herstellung eines peptids mit einem pi grösser als 8 oder kleiner als 5

Country Status (14)

Country Link
US (1) US6303340B1 (de)
EP (3) EP2305815B1 (de)
JP (2) JP4741076B2 (de)
CN (2) CN1242062C (de)
AT (2) ATE529515T1 (de)
AU (1) AU4859599A (de)
CA (1) CA2334160C (de)
CY (2) CY1106110T1 (de)
DE (1) DE69930938T2 (de)
DK (2) DK1714972T3 (de)
ES (2) ES2263278T3 (de)
HK (2) HK1036637A1 (de)
PT (2) PT1095141E (de)
WO (1) WO2000003011A2 (de)

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Publication number Priority date Publication date Assignee Title
DE69628026T2 (de) * 1995-09-07 2004-04-08 Novozymes A/S Nachweis der aktivität von enzymen für detergenzien durch phagen.
ES2525317T3 (es) 2001-06-15 2014-12-22 F. Hoffmann-La Roche Ag Producción recombinante de inhibidores de la fusión antivirales peptídicos
JP4088584B2 (ja) * 2001-07-26 2008-05-21 アドバンスド プロテイン テクノロジーズ コーポレイション 融合タンパク質から目的タンパク質を分離する方法。
US20060148699A1 (en) * 2004-10-04 2006-07-06 Avi Tovi Counterion exchange process for peptides
JP2008054673A (ja) * 2006-08-03 2008-03-13 Hokkaido Univ 組み換え蛋白質の製造方法
US8951185B2 (en) 2007-10-26 2015-02-10 Ams Research Corporation Surgical articles and methods for treating pelvic conditions
US20090035815A1 (en) * 2007-06-29 2009-02-05 Laxmi Srinivas Rao Synthetic Gene for Enhanced Expression in E. Coli
US8609621B2 (en) 2010-11-15 2013-12-17 E I Du Pont De Nemours And Company Acid-cleavable linkers exhibiting altered rates of acid hydrolysis
CN107266554A (zh) * 2012-03-19 2017-10-20 麦德林制药私人有限公司 制备重组血管扩张肽的方法、表达构建体、宿主细胞及重组融合多肽
US20150099860A1 (en) * 2013-10-08 2015-04-09 Proteins Easy Corp Methods for separating and purifying endogenous, exogenous and recombinant proteins/peptides from plants and animals using aqueous-free, anhydrous strategies
CN106928316B (zh) * 2015-12-31 2020-11-20 深圳翰宇药业股份有限公司 一种纯化氧化含二硫键多肽的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8314362D0 (en) * 1983-05-24 1983-06-29 Celltech Ltd Polypeptide and protein products
US4987070A (en) * 1987-03-04 1991-01-22 Suntory Limited Use of a 97 amino acid leader sequence from the E. coli B-galactosidase gene for the production of hanp and hptc as fusion proteins
US5114923A (en) 1988-05-31 1992-05-19 California Biotechnology Inc. Recombinant techniques for production of novel natriuretic and vasodilator peptides
CA1320162C (en) * 1988-08-11 1993-07-13 California Biotechnology Inc. Method for stabilizing heterologous protein expression and vectors for use therein
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
US5202239A (en) * 1990-08-07 1993-04-13 Scios Nova Inc. Expression of recombinant polypeptides with improved purification
DK0573551T3 (da) 1991-02-27 2003-08-04 Micromet Ag Serinrige peptidlinkere
ATE253639T1 (de) * 1991-08-19 2003-11-15 Daiichi Suntory Pharma Co Ltd Verfahren zur herstellung von peptiden
JP3531947B2 (ja) * 1991-08-19 2004-05-31 第一サントリーファーマ株式会社 ペプチドの製造方法
US5589364A (en) 1994-07-29 1996-12-31 Magainin Pharmaceuticals Inc. Recombinant production of biologically active peptides and proteins
JP3995279B2 (ja) * 1994-09-07 2007-10-24 アスビオファーマ株式会社 蛋白の製造方法
CA2198966C (en) * 1996-03-04 2011-06-21 Yuji Suzuki Method for cleaving chimeric protein using processing enzyme
US5851802A (en) * 1996-03-22 1998-12-22 Xoma Corporation Methods for recombinant microbial production of fusion proteins and BPI-derived peptides

Also Published As

Publication number Publication date
CN1521261A (zh) 2004-08-18
DE69930938D1 (de) 2006-05-24
JP2002520018A (ja) 2002-07-09
WO2000003011A2 (en) 2000-01-20
US6303340B1 (en) 2001-10-16
ES2374829T3 (es) 2012-02-22
EP2305815B1 (de) 2014-11-05
CA2334160A1 (en) 2000-01-20
EP2305815A1 (de) 2011-04-06
PT1095141E (pt) 2006-07-31
JP4741076B2 (ja) 2011-08-03
CN1170932C (zh) 2004-10-13
EP1095141B1 (de) 2006-04-19
CA2334160C (en) 2016-09-27
AU4859599A (en) 2000-02-01
PT1714972E (pt) 2011-12-26
CN1242062C (zh) 2006-02-15
CN1313896A (zh) 2001-09-19
DE69930938T2 (de) 2007-01-11
JP5291137B2 (ja) 2013-09-18
CY1106110T1 (el) 2011-06-08
HK1097280A1 (en) 2007-06-22
DK1714972T3 (da) 2012-01-23
ES2263278T3 (es) 2006-12-01
CY1112195T1 (el) 2015-12-09
EP1095141A2 (de) 2001-05-02
EP1714972A2 (de) 2006-10-25
JP2011172575A (ja) 2011-09-08
EP1714972A3 (de) 2008-05-28
HK1036637A1 (en) 2002-01-11
WO2000003011A3 (en) 2000-06-15
DK1095141T3 (da) 2006-07-31
EP1714972B1 (de) 2011-10-19
ATE529515T1 (de) 2011-11-15

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