DE69908631D1 - Vorrichtung zur elektroporation bei der die temperatur während dieses prozesses kontrolliert wird - Google Patents

Vorrichtung zur elektroporation bei der die temperatur während dieses prozesses kontrolliert wird

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Publication number
DE69908631D1
DE69908631D1 DE69908631T DE69908631T DE69908631D1 DE 69908631 D1 DE69908631 D1 DE 69908631D1 DE 69908631 T DE69908631 T DE 69908631T DE 69908631 T DE69908631 T DE 69908631T DE 69908631 D1 DE69908631 D1 DE 69908631D1
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Prior art keywords
cells
low voltage
duration
cell
molecules
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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DE69908631T
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English (en)
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DE69908631T2 (de
Inventor
Singh Nanda
Rejean Laverdiere
A Hofmann
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Genetronics Inc
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Genetronics Inc
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Publication of DE69908631D1 publication Critical patent/DE69908631D1/de
Publication of DE69908631T2 publication Critical patent/DE69908631T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Electromagnetism (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatuses And Processes For Manufacturing Resistors (AREA)
DE69908631T 1998-10-21 1999-09-27 Vorrichtung zur elektroporation bei der die temperatur während dieses prozesses kontrolliert wird Expired - Fee Related DE69908631T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/176,136 US6150148A (en) 1998-10-21 1998-10-21 Electroporation apparatus for control of temperature during the process
US176136 1998-10-21
PCT/US1999/022306 WO2000023563A1 (en) 1998-10-21 1999-09-27 Electroporation apparatus for control of temperature during the process

Publications (2)

Publication Number Publication Date
DE69908631D1 true DE69908631D1 (de) 2003-07-10
DE69908631T2 DE69908631T2 (de) 2004-04-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE69908631T Expired - Fee Related DE69908631T2 (de) 1998-10-21 1999-09-27 Vorrichtung zur elektroporation bei der die temperatur während dieses prozesses kontrolliert wird

Country Status (8)

Country Link
US (2) US6150148A (de)
EP (1) EP1121416B1 (de)
CN (1) CN1324398A (de)
AT (1) ATE242315T1 (de)
AU (1) AU6266699A (de)
CA (1) CA2344325A1 (de)
DE (1) DE69908631T2 (de)
WO (1) WO2000023563A1 (de)

Families Citing this family (150)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2495801A (en) * 1999-12-22 2001-07-09 Innovation And Development Corporation Method and apparatus for targeting localised electroporation
DE10031179A1 (de) * 2000-06-27 2002-01-31 Amaxa Gmbh Verfahren zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms
US7101703B2 (en) * 2001-03-09 2006-09-05 Cornell Research Foundation, Inc. Electrofusion microelectrode
DE10119901A1 (de) * 2001-04-23 2002-10-24 Amaxa Gmbh Schaltungsanordnung zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms
US6994706B2 (en) 2001-08-13 2006-02-07 Minnesota Medical Physics, Llc Apparatus and method for treatment of benign prostatic hyperplasia
US6713292B2 (en) * 2001-12-06 2004-03-30 Bio-Rad Laboratories, Inc. Electroporation cuvette insert for facilitating membrane-based fusion
US20030129716A1 (en) * 2001-12-06 2003-07-10 Bio-Rad Laboratories, Inc. Automatic electroporation optimization system
US8209006B2 (en) * 2002-03-07 2012-06-26 Vgx Pharmaceuticals, Inc. Constant current electroporation device and methods of use
US7245963B2 (en) * 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
US6699712B2 (en) * 2002-03-20 2004-03-02 Bio-Rad Laboratories, Inc. Electroporation chamber
US9982251B2 (en) * 2003-03-14 2018-05-29 Cellectis S.A. Large volume ex vivo electroporation method
US6969604B1 (en) 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
WO2006080934A2 (en) * 2004-04-19 2006-08-03 Invitrogen Corporation Electroporation apparatus and methods
US20050277183A1 (en) * 2004-05-18 2005-12-15 Ronald Lee Electroporation cuvette
EP1607484B1 (de) * 2004-06-14 2014-04-02 Lonza Cologne GmbH Verfahren und Schaltungsanordnung zur Behandlung von biologischem Material
US7915044B2 (en) * 2005-12-22 2011-03-29 Cornell Research Foundation, Inc. Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components
KR101248799B1 (ko) 2006-04-28 2013-04-02 젤티크 애스세틱스, 인코포레이티드. 피하 지질 과다 세포의 개선된 냉각을 위한 치료 장치와 함께 사용하기 위한 동결 방지제
US8192474B2 (en) 2006-09-26 2012-06-05 Zeltiq Aesthetics, Inc. Tissue treatment methods
US9132031B2 (en) 2006-09-26 2015-09-15 Zeltiq Aesthetics, Inc. Cooling device having a plurality of controllable cooling elements to provide a predetermined cooling profile
US20080077201A1 (en) 2006-09-26 2008-03-27 Juniper Medical, Inc. Cooling devices with flexible sensors
US7687267B2 (en) * 2006-09-30 2010-03-30 Rational Biotechnology Inc. High-throughput cell transfection device and methods of using thereof
PT2066399T (pt) 2006-10-17 2019-01-11 Inovio Pharmaceuticals Inc Dispositivos de eletroporação e métodos de usar os mesmos para eletroporação de células em mamíferos
ES2534579T3 (es) * 2007-02-23 2015-04-24 Lonza Cologne Gmbh Dispositivo y procedimiento para estabilizar el flujo a través de una cámara
US20080287839A1 (en) 2007-05-18 2008-11-20 Juniper Medical, Inc. Method of enhanced removal of heat from subcutaneous lipid-rich cells and treatment apparatus having an actuator
US8523927B2 (en) 2007-07-13 2013-09-03 Zeltiq Aesthetics, Inc. System for treating lipid-rich regions
EP3488833A1 (de) 2007-08-21 2019-05-29 Zeltiq Aesthetics, Inc. Überwachung der kühlung von subkutanen lipidreichen zellen, z.b. kühlung von fettgewebe
US8043838B2 (en) * 2008-02-20 2011-10-25 Bio-Rad Laboratories, Inc. Electroporation cuvette with spatially variable electric field
WO2009123564A1 (en) * 2008-04-03 2009-10-08 Nanyang Polytechnic Apparatus for electroporation with cooling system
US10117707B2 (en) 2008-04-29 2018-11-06 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US10238447B2 (en) 2008-04-29 2019-03-26 Virginia Tech Intellectual Properties, Inc. System and method for ablating a tissue site by electroporation with real-time monitoring of treatment progress
CA2722296A1 (en) 2008-04-29 2009-11-05 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation to create tissue scaffolds
US11254926B2 (en) 2008-04-29 2022-02-22 Virginia Tech Intellectual Properties, Inc. Devices and methods for high frequency electroporation
US9867652B2 (en) 2008-04-29 2018-01-16 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds
US9283051B2 (en) 2008-04-29 2016-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating a treatment volume for administering electrical-energy based therapies
US10245098B2 (en) 2008-04-29 2019-04-02 Virginia Tech Intellectual Properties, Inc. Acute blood-brain barrier disruption using electrical energy based therapy
US11272979B2 (en) 2008-04-29 2022-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US9198733B2 (en) 2008-04-29 2015-12-01 Virginia Tech Intellectual Properties, Inc. Treatment planning for electroporation-based therapies
US10702326B2 (en) 2011-07-15 2020-07-07 Virginia Tech Intellectual Properties, Inc. Device and method for electroporation based treatment of stenosis of a tubular body part
US10272178B2 (en) 2008-04-29 2019-04-30 Virginia Tech Intellectual Properties Inc. Methods for blood-brain barrier disruption using electrical energy
US8992517B2 (en) 2008-04-29 2015-03-31 Virginia Tech Intellectual Properties Inc. Irreversible electroporation to treat aberrant cell masses
US8603073B2 (en) 2008-12-17 2013-12-10 Zeltiq Aesthetics, Inc. Systems and methods with interrupt/resume capabilities for treating subcutaneous lipid-rich cells
NO2208780T3 (de) 2009-01-20 2018-03-31
EP2208778A1 (de) 2009-01-20 2010-07-21 Lonza Cologne AG Verfahren und Vorrichtung zur elektrischen Behandlung von Reaktionsräumen
US8632534B2 (en) * 2009-04-03 2014-01-21 Angiodynamics, Inc. Irreversible electroporation (IRE) for congestive obstructive pulmonary disease (COPD)
US11638603B2 (en) 2009-04-09 2023-05-02 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
US11382681B2 (en) 2009-04-09 2022-07-12 Virginia Tech Intellectual Properties, Inc. Device and methods for delivery of high frequency electrical pulses for non-thermal ablation
CA2975717C (en) 2009-04-30 2020-04-28 Zeltiq Aesthetics, Inc. Device, system and method of removing heat from subcutaneous lipid-rich cells
US8903488B2 (en) 2009-05-28 2014-12-02 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US9895189B2 (en) 2009-06-19 2018-02-20 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
CN102656260A (zh) * 2009-10-19 2012-09-05 瑞生生物技术有限公司 用于诱导自调节细胞电穿孔的方法、装置和设备
CN102791227A (zh) 2010-01-25 2012-11-21 斯尔替克美学股份有限公司 用于通过相变冷却剂从皮下富脂细胞无创去除热量的家用施用器以及相关的装置、系统和方法
EP2399984B1 (de) 2010-06-22 2013-03-20 Lonza Cologne GmbH Verfahren und Elektrodenanordnung zur Behandlung von adhärenten Zellen
US8676338B2 (en) 2010-07-20 2014-03-18 Zeltiq Aesthetics, Inc. Combined modality treatment systems, methods and apparatus for body contouring applications
WO2012041867A2 (en) 2010-09-27 2012-04-05 China Agricultural University Combined antigen and dna vaccine for preventing and treating autoimmune diseases
EP2627274B1 (de) 2010-10-13 2022-12-14 AngioDynamics, Inc. System zur elektrischen ableitung des gewebes eines patienten
WO2012088149A2 (en) 2010-12-20 2012-06-28 Virginia Tech Intellectual Properties, Inc. High-frequency electroporation for cancer therapy
US9078665B2 (en) 2011-09-28 2015-07-14 Angiodynamics, Inc. Multiple treatment zone ablation probe
CN103239734B (zh) 2012-02-10 2016-02-24 北京艾棣维欣生物技术有限公司 用于预防和/或治疗呼吸道合胞病毒感染的疫苗
WO2014093897A1 (en) 2012-12-13 2014-06-19 The Trustees Of The University Of Pennsylvania Wt1 vaccine
US9888956B2 (en) 2013-01-22 2018-02-13 Angiodynamics, Inc. Integrated pump and generator device and method of use
US9545523B2 (en) 2013-03-14 2017-01-17 Zeltiq Aesthetics, Inc. Multi-modality treatment systems, methods and apparatus for altering subcutaneous lipid-rich tissue
US9844460B2 (en) 2013-03-14 2017-12-19 Zeltiq Aesthetics, Inc. Treatment systems with fluid mixing systems and fluid-cooled applicators and methods of using the same
US10364451B2 (en) 2013-05-30 2019-07-30 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
US9592303B2 (en) 2013-05-30 2017-03-14 Duke University Enzyme-catalyzed synthesis of site-specific and stoichiometric biomolecule-polymer conjugates
US10392611B2 (en) 2013-05-30 2019-08-27 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
CN105744953B (zh) 2013-10-07 2020-01-17 宾夕法尼亚大学理事会 具有作为佐剂的白介素-33的疫苗
WO2015070027A1 (en) 2013-11-07 2015-05-14 University Of Southern California Use of ikk epsilon inhibitors to activate nfat and t cell response
WO2015073291A1 (en) 2013-11-14 2015-05-21 The Trustees Of The University Of Pennsylvania Hiv-1 env dna vaccine plus protein boost
KR20230122181A (ko) 2013-11-29 2023-08-22 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 중동 호흡기 증후군 코로나바이러스(MERS-CoV) 백신
KR102395498B1 (ko) 2014-01-06 2022-05-09 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 Pd1 및 pdl1 항체 및 백신 조합 및 면역요법을 위한 이들의 사용
EP3099261A2 (de) 2014-01-31 2016-12-07 Zeltiq Aesthetics, Inc. Behandlungssysteme zur behandlung von cellulitis durch kühlung
US10675176B1 (en) 2014-03-19 2020-06-09 Zeltiq Aesthetics, Inc. Treatment systems, devices, and methods for cooling targeted tissue
USD777338S1 (en) 2014-03-20 2017-01-24 Zeltiq Aesthetics, Inc. Cryotherapy applicator for cooling tissue
EP2940120B1 (de) 2014-05-02 2018-08-01 Lonza Cologne GmbH Vorrichtung und Verfahren für großvolumige Transfektion
CN106715682A (zh) 2014-05-12 2017-05-24 弗吉尼亚暨州立大学知识产权公司 使用脉冲电场选择性调节细胞的胞内影响
US10952891B1 (en) 2014-05-13 2021-03-23 Zeltiq Aesthetics, Inc. Treatment systems with adjustable gap applicators and methods for cooling tissue
US10568759B2 (en) 2014-08-19 2020-02-25 Zeltiq Aesthetics, Inc. Treatment systems, small volume applicators, and methods for treating submental tissue
US10935174B2 (en) 2014-08-19 2021-03-02 Zeltiq Aesthetics, Inc. Stress relief couplings for cryotherapy apparatuses
WO2016054003A1 (en) 2014-10-01 2016-04-07 The Trustees Of The University Of Pennsylvania Vaccines having an antigen and interleukin-21 as an adjuvant
US10694972B2 (en) 2014-12-15 2020-06-30 Virginia Tech Intellectual Properties, Inc. Devices, systems, and methods for real-time monitoring of electrophysical effects during tissue treatment
US11116838B2 (en) 2015-01-29 2021-09-14 The Trustees Of The University Of Pennsylvania Checkpoint inhibitor and vaccine combinations and use of same for immunotherapy
US10385115B2 (en) 2015-03-26 2019-08-20 Duke University Fibronectin type III domain-based fusion proteins
EA037478B1 (ru) 2015-08-04 2021-04-01 Дьюк Юниверсити Генетически кодируемые внутренне неупорядоченные полимеры-"невидимки" для доставки и способы их применения
US11154418B2 (en) 2015-10-19 2021-10-26 Zeltiq Aesthetics, Inc. Vascular treatment systems, cooling devices, and methods for cooling vascular structures
US11752213B2 (en) 2015-12-21 2023-09-12 Duke University Surfaces having reduced non-specific binding and antigenicity
US20190015520A1 (en) 2015-12-21 2019-01-17 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
KR20180099747A (ko) * 2015-12-28 2018-09-05 이노비오 파마수티컬즈, 인크. 개선된 신호 발생기를 가진 전기천공 디바이스
CA3009414A1 (en) 2016-01-07 2017-07-13 Zeltiq Aesthetics, Inc. Temperature-dependent adhesion between applicator and skin during cooling of tissue
US10765552B2 (en) 2016-02-18 2020-09-08 Zeltiq Aesthetics, Inc. Cooling cup applicators with contoured heads and liner assemblies
WO2017173398A1 (en) 2016-04-01 2017-10-05 Duke University Alpha-helical peptide nanofibers as a self-adjuvanting vaccine platform
US20190284263A1 (en) 2016-04-29 2019-09-19 Inovio Pharmaceuticals, Inc. In vivo use of chondroitinase and/or hyaluronidase to enhance delivery of an agent
US11382790B2 (en) 2016-05-10 2022-07-12 Zeltiq Aesthetics, Inc. Skin freezing systems for treating acne and skin conditions
US10555831B2 (en) 2016-05-10 2020-02-11 Zeltiq Aesthetics, Inc. Hydrogel substances and methods of cryotherapy
US10682297B2 (en) 2016-05-10 2020-06-16 Zeltiq Aesthetics, Inc. Liposomes, emulsions, and methods for cryotherapy
US11467156B2 (en) 2016-06-01 2022-10-11 Duke University Nonfouling biosensors
US10233419B2 (en) 2016-06-30 2019-03-19 Zymergen Inc. Apparatuses and methods for electroporation
US11135301B2 (en) 2016-09-14 2021-10-05 Duke University Triblock polypeptide-based nanoparticles for the delivery of hydrophilic drugs
EP3515928A4 (de) 2016-09-23 2020-04-01 Duke University Unstrukturierte nichtrepititive polypeptide mit lcst-verhalten
US10905492B2 (en) 2016-11-17 2021-02-02 Angiodynamics, Inc. Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode
US10376495B2 (en) 2016-11-23 2019-08-13 University Of South Florida Small molecules that mimic or antagonize actions of granulocyte colony-stimulating-factor (G-CSF)
US11648200B2 (en) 2017-01-12 2023-05-16 Duke University Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature triggered hierarchical self-assembly
US10813935B2 (en) 2017-02-23 2020-10-27 Transgenex Nanobiotech, Inc. Methods and compositions for treating drug resistance in cancer
WO2018156989A1 (en) 2017-02-24 2018-08-30 University Of South Florida The hsp90 activator aha1 drives production of pathological tau aggregates
US11076879B2 (en) 2017-04-26 2021-08-03 Zeltiq Aesthetics, Inc. Shallow surface cryotherapy applicators and related technology
US11554097B2 (en) 2017-05-15 2023-01-17 Duke University Recombinant production of hybrid lipid-biopolymer materials that self-assemble and encapsulate agents
EP3658168A4 (de) 2017-06-30 2021-07-14 Duke University Ordnung und unordnung als designprinzip für auf stimuli ansprechende biopolymernetzwerke
KR102424850B1 (ko) 2017-06-30 2022-07-22 인스크립타 인코포레이티드 자동 세포 처리 방법, 모듈, 기기 및 시스템
GB201710973D0 (en) 2017-07-07 2017-08-23 Avacta Life Sciences Ltd Scaffold proteins
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
CN111372650A (zh) 2017-09-30 2020-07-03 因思科瑞普特公司 流通式电穿孔仪器
US11607537B2 (en) 2017-12-05 2023-03-21 Virginia Tech Intellectual Properties, Inc. Method for treating neurological disorders, including tumors, with electroporation
US11311329B2 (en) 2018-03-13 2022-04-26 Virginia Tech Intellectual Properties, Inc. Treatment planning for immunotherapy based treatments using non-thermal ablation techniques
US11925405B2 (en) 2018-03-13 2024-03-12 Virginia Tech Intellectual Properties, Inc. Treatment planning system for immunotherapy enhancement via non-thermal ablation
CN112204131A (zh) 2018-03-29 2021-01-08 因思科瑞普特公司 用于诱导和转化的细胞生长速率的自动化控制
WO2019200004A1 (en) * 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10508273B2 (en) 2018-04-24 2019-12-17 Inscripta, Inc. Methods for identifying selective binding pairs
CN114854720A (zh) 2018-06-30 2022-08-05 因思科瑞普特公司 用于改进活细胞中编辑序列的检测的仪器、模块和方法
CA3107932A1 (en) 2018-07-31 2020-02-06 Zeltiq Aesthetics, Inc. Methods, devices, and systems for improving skin characteristics
WO2020028806A1 (en) 2018-08-02 2020-02-06 Duke University Dual agonist fusion proteins
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
CA3108892A1 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
DE102018128723A1 (de) * 2018-11-15 2020-05-20 Endress+Hauser Conducta Gmbh+Co. Kg Küvette, vorzugsweise Durchflussküvette für ein optisches Messgerät und Verfahren zu dessen Betrieb
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
CN114008070A (zh) 2019-06-21 2022-02-01 因思科瑞普特公司 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US11950835B2 (en) 2019-06-28 2024-04-09 Virginia Tech Intellectual Properties, Inc. Cycled pulsing to mitigate thermal damage for multi-electrode irreversible electroporation therapy
US20220228191A1 (en) * 2019-07-02 2022-07-21 Horiba Advanced Techno, Co., Ltd. Biological sample analysis apparatus and biological sample analysis method
US11512314B2 (en) 2019-07-12 2022-11-29 Duke University Amphiphilic polynucleotides
CN110511869B (zh) * 2019-08-08 2023-06-09 吉林大学 细胞培养方法及能产生可变脉冲电场的细胞培养装置
TW202128775A (zh) 2019-10-16 2021-08-01 英商阿法克塔生命科學有限公司 PD-L1抑制劑-TGFβ抑制劑雙特異性藥物部分
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US11225674B2 (en) 2020-01-27 2022-01-18 Inscripta, Inc. Electroporation modules and instrumentation
KR20220157969A (ko) 2020-02-25 2022-11-29 이노비오 파마수티컬즈, 인크. 코로나바이러스 백신 및 사용 방법
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
CN115803051A (zh) 2020-05-14 2023-03-14 艾诺奥医药品有限公司 用于复发性呼吸道乳头状瘤病的疫苗及其使用方法
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
GB202101299D0 (en) 2020-06-09 2021-03-17 Avacta Life Sciences Ltd Diagnostic polypetides and methods
CN116133668A (zh) 2020-06-12 2023-05-16 罗切斯特大学 ACE-tRNA的编码和表达
US12076071B2 (en) 2020-08-14 2024-09-03 Kardium Inc. Systems and methods for treating tissue with pulsed field ablation
EP4001396A1 (de) * 2020-11-17 2022-05-25 Bühler AG Vorrichtung zur behandlung von zellen in einem bypass
WO2022234003A1 (en) 2021-05-07 2022-11-10 Avacta Life Sciences Limited Cd33 binding polypeptides with stefin a protein
EP4412711A1 (de) 2021-10-07 2024-08-14 Avacta Life Sciences Limited Serumhalbwertszeiterweiterte pd-l1-bindende polypeptide
EP4413038A1 (de) 2021-10-07 2024-08-14 Avacta Life Sciences Limited Pd-l1-bindende affimere
WO2023150753A1 (en) 2022-02-07 2023-08-10 University Of Rochester Optimized sequences for enhanced trna expression or/and nonsense mutation suppression
WO2024102187A1 (en) 2022-11-07 2024-05-16 Pinetree Therapeutics, Inc. Combination therapy comprising bispecific antibodies comprising an nrp1 binding domain
WO2024129459A1 (en) 2022-12-16 2024-06-20 University Of Rochester Repairmen! of barrier dysfunction in esophagus

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2798459B2 (ja) * 1988-01-21 1998-09-17 マサチユセツツ・インスチチユート・オブ・テクノロジー エレクトロポレーションを利用した診断装置及び分子の組織内移動装置
BE1004328A3 (fr) * 1990-05-16 1992-11-03 Scient Equipment Design & Dev Procede et dispositif de permeabilisation de cellules vivantes.
US5185071A (en) * 1990-10-30 1993-02-09 Board Of Regents, The University Of Texas System Programmable electrophoresis with integrated and multiplexed control
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5422272A (en) * 1993-07-14 1995-06-06 Andrew A. Papp Improvements to apparatus and method for electroporation
US5869326A (en) * 1996-09-09 1999-02-09 Genetronics, Inc. Electroporation employing user-configured pulsing scheme

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CN1324398A (zh) 2001-11-28
EP1121416A1 (de) 2001-08-08
US6150148A (en) 2000-11-21
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