DE69907240T2 - Feste phytase-zusammensetzungen - Google Patents
Feste phytase-zusammensetzungenInfo
- Publication number
- DE69907240T2 DE69907240T2 DE69907240T DE69907240T DE69907240T2 DE 69907240 T2 DE69907240 T2 DE 69907240T2 DE 69907240 T DE69907240 T DE 69907240T DE 69907240 T DE69907240 T DE 69907240T DE 69907240 T2 DE69907240 T2 DE 69907240T2
- Authority
- DE
- Germany
- Prior art keywords
- units
- phytase
- composition
- csl
- phytase activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010011619 6-Phytase Proteins 0.000 title claims abstract description 99
- 229940085127 phytase Drugs 0.000 title claims abstract description 90
- 239000000203 mixture Substances 0.000 title claims abstract description 50
- 108090000790 Enzymes Proteins 0.000 claims abstract description 36
- 102000004190 Enzymes Human genes 0.000 claims abstract description 36
- 229940088598 enzyme Drugs 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 28
- 229920002472 Starch Polymers 0.000 claims abstract description 26
- 239000007787 solid Substances 0.000 claims abstract description 15
- 150000002016 disaccharides Chemical class 0.000 claims abstract description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 12
- 235000005822 corn Nutrition 0.000 claims abstract description 12
- 235000019698 starch Nutrition 0.000 claims abstract description 10
- 239000008107 starch Substances 0.000 claims abstract description 9
- 230000000694 effects Effects 0.000 claims description 42
- 229940100445 wheat starch Drugs 0.000 claims description 15
- 239000008247 solid mixture Substances 0.000 claims description 14
- 238000003556 assay Methods 0.000 claims description 10
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 239000012876 carrier material Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000008202 granule composition Substances 0.000 claims description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 2
- 239000011707 mineral Substances 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 229940088594 vitamin Drugs 0.000 claims description 2
- 229930003231 vitamin Natural products 0.000 claims description 2
- 235000013343 vitamin Nutrition 0.000 claims description 2
- 241000209149 Zea Species 0.000 claims 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 48
- 235000014655 lactic acid Nutrition 0.000 abstract description 24
- 239000004310 lactic acid Substances 0.000 abstract description 23
- 240000008042 Zea mays Species 0.000 abstract description 10
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 abstract description 6
- 239000008101 lactose Substances 0.000 abstract description 6
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 abstract description 5
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 abstract description 5
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 abstract description 5
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 abstract description 5
- 239000008187 granular material Substances 0.000 description 43
- 239000000523 sample Substances 0.000 description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 28
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 16
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 15
- 235000002949 phytic acid Nutrition 0.000 description 15
- 239000000243 solution Substances 0.000 description 15
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 14
- 238000003860 storage Methods 0.000 description 13
- 239000000758 substrate Substances 0.000 description 13
- 239000007788 liquid Substances 0.000 description 12
- 229940068041 phytic acid Drugs 0.000 description 12
- 239000000467 phytic acid Substances 0.000 description 12
- 239000002245 particle Substances 0.000 description 11
- 239000008351 acetate buffer Substances 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 235000008504 concentrate Nutrition 0.000 description 9
- 239000012141 concentrate Substances 0.000 description 9
- 238000005469 granulation Methods 0.000 description 9
- 230000003179 granulation Effects 0.000 description 9
- 229920001213 Polysorbate 20 Polymers 0.000 description 8
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 8
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 8
- 239000004615 ingredient Substances 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 235000017281 sodium acetate Nutrition 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 229910019142 PO4 Inorganic materials 0.000 description 6
- 229920000136 polysorbate Polymers 0.000 description 6
- 239000000872 buffer Substances 0.000 description 5
- 239000008367 deionised water Substances 0.000 description 5
- 229910021641 deionized water Inorganic materials 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 5
- 239000010452 phosphate Substances 0.000 description 5
- 239000004033 plastic Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- 239000012089 stop solution Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 239000005995 Aluminium silicate Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 244000024675 Eruca sativa Species 0.000 description 4
- 235000014755 Eruca sativa Nutrition 0.000 description 4
- 235000019482 Palm oil Nutrition 0.000 description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 4
- FENRSEGZMITUEF-ATTCVCFYSA-E [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] FENRSEGZMITUEF-ATTCVCFYSA-E 0.000 description 4
- 238000005054 agglomeration Methods 0.000 description 4
- 230000002776 aggregation Effects 0.000 description 4
- 235000012211 aluminium silicate Nutrition 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- -1 by Spray drying Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 239000000945 filler Substances 0.000 description 4
- 150000003893 lactate salts Chemical class 0.000 description 4
- MEFBJEMVZONFCJ-UHFFFAOYSA-N molybdate Chemical compound [O-][Mo]([O-])(=O)=O MEFBJEMVZONFCJ-UHFFFAOYSA-N 0.000 description 4
- 239000002540 palm oil Substances 0.000 description 4
- 239000001632 sodium acetate Substances 0.000 description 4
- 229940083982 sodium phytate Drugs 0.000 description 4
- BDKLKNJTMLIAFE-UHFFFAOYSA-N 2-(3-fluorophenyl)-1,3-oxazole-4-carbaldehyde Chemical compound FC1=CC=CC(C=2OC=C(C=O)N=2)=C1 BDKLKNJTMLIAFE-UHFFFAOYSA-N 0.000 description 3
- 241000235349 Ascomycota Species 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 3
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000011230 binding agent Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 229910017604 nitric acid Inorganic materials 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 229940087562 sodium acetate trihydrate Drugs 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000003760 tallow Substances 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- 229910003208 (NH4)6Mo7O24·4H2O Inorganic materials 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002390 adhesive tape Substances 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- UNTBPXHCXVWYOI-UHFFFAOYSA-O azanium;oxido(dioxo)vanadium Chemical compound [NH4+].[O-][V](=O)=O UNTBPXHCXVWYOI-UHFFFAOYSA-O 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000011536 extraction buffer Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000003988 headspace gas chromatography Methods 0.000 description 2
- 235000014666 liquid concentrate Nutrition 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 description 2
- 235000011152 sodium sulphate Nutrition 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- LSGOVYNHVSXFFJ-UHFFFAOYSA-N vanadate(3-) Chemical compound [O-][V]([O-])([O-])=O LSGOVYNHVSXFFJ-UHFFFAOYSA-N 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- KQROHCSYOGBQGJ-UHFFFAOYSA-N 5-Hydroxytryptophol Chemical compound C1=C(O)C=C2C(CCO)=CNC2=C1 KQROHCSYOGBQGJ-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 235000019890 Amylum Nutrition 0.000 description 1
- 101000649961 Arabidopsis thaliana Inositol-phosphate phosphatase Proteins 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241001513093 Aspergillus awamori Species 0.000 description 1
- 241000228195 Aspergillus ficuum Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241001465318 Aspergillus terreus Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 241000234435 Lilium Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 241001676646 Peniophora lycii Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 241001123650 Schwanniomyces occidentalis Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 241001313536 Thermothelomyces thermophila Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- SNAAJJQQZSMGQD-UHFFFAOYSA-N aluminum magnesium Chemical compound [Mg].[Al] SNAAJJQQZSMGQD-UHFFFAOYSA-N 0.000 description 1
- 235000019770 animal feed premixes Nutrition 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 239000004067 bulking agent Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 229920005551 calcium lignosulfonate Polymers 0.000 description 1
- RYAGRZNBULDMBW-UHFFFAOYSA-L calcium;3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Ca+2].COC1=CC=CC(CC(CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O RYAGRZNBULDMBW-UHFFFAOYSA-L 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000004464 cereal grain Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000011247 coating layer Substances 0.000 description 1
- 239000011162 core material Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007824 enzymatic assay Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012520 frozen sample Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000010440 gypsum Substances 0.000 description 1
- 229910052602 gypsum Inorganic materials 0.000 description 1
- 239000008172 hydrogenated vegetable oil Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 102000006029 inositol monophosphatase Human genes 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000004014 plasticizer Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 235000012249 potassium ferrocyanide Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940076788 pyruvate Drugs 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- RPACBEVZENYWOL-XFULWGLBSA-M sodium;(2r)-2-[6-(4-chlorophenoxy)hexyl]oxirane-2-carboxylate Chemical compound [Na+].C=1C=C(Cl)C=CC=1OCCCCCC[C@]1(C(=O)[O-])CO1 RPACBEVZENYWOL-XFULWGLBSA-M 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 description 1
- 150000008501 α-D-glucopyranosides Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/98—Preparation of granular or free-flowing enzyme compositions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Fodder In General (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199801251 | 1998-10-02 | ||
| PCT/DK1999/000516 WO2000020569A1 (en) | 1998-10-02 | 1999-10-01 | Solid phytase compositions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69907240D1 DE69907240D1 (de) | 2003-05-28 |
| DE69907240T2 true DE69907240T2 (de) | 2003-11-27 |
Family
ID=8102729
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69907240T Expired - Lifetime DE69907240T2 (de) | 1998-10-02 | 1999-10-01 | Feste phytase-zusammensetzungen |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1117771B1 (https=) |
| JP (1) | JP4455764B2 (https=) |
| CN (1) | CN1197963C (https=) |
| AT (1) | ATE238415T1 (https=) |
| AU (1) | AU761363B2 (https=) |
| BR (1) | BR9914217A (https=) |
| CA (1) | CA2341484C (https=) |
| DE (1) | DE69907240T2 (https=) |
| DK (1) | DK1117771T3 (https=) |
| WO (1) | WO2000020569A1 (https=) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
| AU4056700A (en) | 1999-03-31 | 2000-10-16 | Cornell Research Foundation Inc. | Phosphatases with improved phytase activity |
| US6841370B1 (en) | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
| AU2002356880A1 (en) | 2001-10-31 | 2003-05-12 | Phytex, Llc | Phytase-containing animal food and method |
| JP4426307B2 (ja) | 2002-02-08 | 2010-03-03 | ノボザイムス アクティーゼルスカブ | フィターゼ変異体 |
| US6815486B2 (en) | 2002-04-12 | 2004-11-09 | Dow Corning Corporation | Thermally conductive phase change materials and methods for their preparation and use |
| WO2004024885A2 (en) | 2002-09-13 | 2004-03-25 | Cornell Research Foundation, Inc. | Using mutations to improve aspergillus phytases |
| AR047658A1 (es) | 2004-02-03 | 2006-02-01 | Cargill Inc | Concentrado de proteinas y corriente acuosa con carbohidratos hidrosolubles |
| DK2258209T3 (da) * | 2004-09-27 | 2015-08-31 | Novozymes As | Phytasegranuler i dyrefoder |
| MX285771B (es) | 2004-10-04 | 2011-04-07 | Novozymes As | Polipeptidos que tienen avtividad de fitasa y polinucleotidos queridos mama y papa: codifican los mismos. |
| AR050895A1 (es) | 2004-10-04 | 2006-11-29 | Novozymes As | Polipeptidos que tienen actividad de fitasa y polinucleotidos que los codifican |
| EP2497374A3 (en) * | 2005-10-12 | 2013-11-27 | Danisco US Inc. | Stable, durable granules with active agents |
| US7919297B2 (en) | 2006-02-21 | 2011-04-05 | Cornell Research Foundation, Inc. | Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase |
| US20090317515A1 (en) * | 2006-03-10 | 2009-12-24 | Basf Se | Solid enzyme formulations and process for their preparation |
| MX2008012632A (es) | 2006-04-04 | 2008-10-13 | Novozymes As | Variantes de fitasa. |
| WO2008017066A2 (en) | 2006-08-03 | 2008-02-07 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
| CN101500430B (zh) | 2006-08-07 | 2014-02-19 | 诺维信公司 | 用于动物饲料的酶团粒 |
| ES2577430T3 (es) | 2006-08-07 | 2016-07-14 | Novozymes A/S | Gránulos de enzima para pienso para animales |
| PL2129781T3 (pl) | 2007-03-26 | 2014-07-31 | Novozymes As | Fitaza z Hafnia |
| ES2636369T3 (es) | 2008-09-26 | 2017-10-05 | Novozymes A/S | Variantes de fitasa de Hafnia |
| WO2011014458A1 (en) | 2009-07-28 | 2011-02-03 | Novozymes, Inc. | Polypeptides having phytase activity and polynucleotides encoding same |
| CA2794113A1 (en) | 2010-03-26 | 2011-09-29 | Novozymes A/S | Thermostable phytase variants |
| CN103874657A (zh) | 2011-08-16 | 2014-06-18 | 索尔维公司 | 制造含LiPO2F2和LiPF6的混合物 |
| CN102511665B (zh) * | 2011-12-29 | 2013-06-05 | 青岛玛斯特生物技术有限公司 | 一种植酸酶包衣微丸及其制备方法 |
| DK3699271T3 (da) * | 2012-02-07 | 2024-12-09 | Danisco Us Inc | Granulat- og pelletsammensætninger, der omfatter phytase og phytinsyre, og fremgangsmåde til fremstilling deraf |
| GB201308828D0 (en) | 2013-03-12 | 2013-07-03 | Verenium Corp | Phytase |
| GB201308843D0 (en) * | 2013-03-14 | 2013-07-03 | Verenium Corp | Phytase formulation |
| EP3027762A4 (en) | 2013-07-25 | 2017-06-28 | BASF Enzymes LLC | Phytase |
| CN109196098A (zh) | 2016-05-24 | 2019-01-11 | 诺维信公司 | 包含具有半乳聚糖酶活性的多肽和具有β-半乳糖苷酶活性的多肽的组合物 |
| BR112018073890A2 (pt) | 2016-05-24 | 2019-02-26 | Novozymes As | composição, grânulo, aditivo de ração para animal, ração para animal, formulação líquida, uso da composição, do grânulo, do aditivo de ração para animal ou da formulação líquida, polipeptídeo isolado, métodos para liberar galactose de material à base de planta, para melhorar um ou mais parâmetros de desempenho de um animal e para produzir o polipeptídeo, polinucleotídeo, construto de ácido nucleico ou vetor de expressão, e, célula hospedeira recombinante. |
| WO2017202979A1 (en) | 2016-05-24 | 2017-11-30 | Novozymes A/S | Polypeptides having alpha-galactosidase activity and polynucleotides encoding same |
| US10808268B2 (en) | 2016-05-24 | 2020-10-20 | Novozymes A/S | Polypeptides having alpha-galactosidase activity and polynucleotides encoding same |
| WO2018007153A1 (en) | 2016-07-08 | 2018-01-11 | Novozymes A/S | Polypeptides having xylanase activity and polynucleotides encoding same |
| CA3029113A1 (en) | 2016-07-08 | 2018-01-11 | Novozymes A/S | Xylanase variants and polynucleotides encoding same |
| US10986850B2 (en) | 2016-12-21 | 2021-04-27 | Novozymes A/S | Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof |
| US11499144B2 (en) | 2017-06-22 | 2022-11-15 | Novozymes A/S | Xylanase variants and polynucleotides encoding same |
| AU2018322865B2 (en) | 2017-09-01 | 2023-11-09 | Novozymes A/S | Animal feed additives comprising polypeptide having protease activity and uses thereof |
| EP4501130A3 (en) | 2017-09-01 | 2025-04-23 | Novozymes A/S | Animal feed additives comprising a polypeptide having protease activity and uses thereof |
| US20210076704A1 (en) | 2017-12-20 | 2021-03-18 | Dsm Ip Assets B.V. | Animal feed compositions and uses thereof |
| US20200337337A1 (en) | 2017-12-20 | 2020-10-29 | Dsm Ip Assets B.V. | Animal feed compositions comprising muramidase and uses thereof |
| EP3737769A1 (en) | 2018-01-11 | 2020-11-18 | Novozymes A/S | Animal feed compositions and uses thereof |
| US12133542B2 (en) | 2018-04-25 | 2024-11-05 | Novozymes A/S | Animal feed compositions and uses thereof |
| EP3852547A1 (en) | 2018-09-17 | 2021-07-28 | DSM IP Assets B.V. | Animal feed compositions and uses thereof |
| MX2021003035A (es) | 2018-09-17 | 2021-08-11 | Dsm Ip Assets Bv | Composiciones de alimento animal y usos de las mismas. |
| EP4581938A3 (en) | 2018-09-17 | 2025-08-20 | DSM IP Assets B.V. | Animal feed compositions and uses thereof |
| MX2021003074A (es) | 2018-09-17 | 2021-06-23 | Dsm Ip Assets Bv | Composiciones de alimento animal y usos de las mismas. |
| WO2020115179A1 (en) | 2018-12-05 | 2020-06-11 | Novozymes A/S | Use of an enzyme granule |
| WO2021078839A1 (en) | 2019-10-22 | 2021-04-29 | Novozymes A/S | Animal feed composition |
| EP4149279A1 (en) | 2020-05-15 | 2023-03-22 | DSM IP Assets B.V. | A method for improving the nutritional value of animal feed |
| CN115666262A (zh) | 2020-05-18 | 2023-01-31 | 帝斯曼知识产权资产管理有限公司 | 动物饲料组合物 |
| EP4152946A1 (en) | 2020-05-18 | 2023-03-29 | DSM IP Assets B.V. | Animal feed compositions |
| BR112023002578A2 (pt) | 2020-08-13 | 2023-03-14 | Novozymes As | Variantes de fitase e polinucleotídeos codificando as mesmas |
| CN116600651A (zh) | 2020-10-07 | 2023-08-15 | 诺维信公司 | 动物饲料中益生菌的酶保存 |
| EP4228424A1 (en) | 2020-10-15 | 2023-08-23 | DSM IP Assets B.V. | Methods of modulating gastrointestinal metabolites |
| CN114958803B (zh) * | 2022-05-18 | 2024-06-04 | 阳原县仁恒精细粘土有限责任公司 | 植酸酶发酵生产方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1159208A (zh) * | 1995-07-28 | 1997-09-10 | 吉斯特·布罗卡迪斯股份有限公司 | 盐稳定化酶制剂 |
| WO1997039116A1 (en) * | 1996-04-12 | 1997-10-23 | Novo Nordisk A/S | Enzyme-containing granules and process for the production thereof |
-
1999
- 1999-10-01 EP EP99945962A patent/EP1117771B1/en not_active Expired - Lifetime
- 1999-10-01 AU AU58505/99A patent/AU761363B2/en not_active Expired
- 1999-10-01 AT AT99945962T patent/ATE238415T1/de not_active IP Right Cessation
- 1999-10-01 BR BR9914217-1A patent/BR9914217A/pt not_active Application Discontinuation
- 1999-10-01 CA CA002341484A patent/CA2341484C/en not_active Expired - Lifetime
- 1999-10-01 DE DE69907240T patent/DE69907240T2/de not_active Expired - Lifetime
- 1999-10-01 DK DK99945962T patent/DK1117771T3/da active
- 1999-10-01 WO PCT/DK1999/000516 patent/WO2000020569A1/en not_active Ceased
- 1999-10-01 CN CNB998114618A patent/CN1197963C/zh not_active Expired - Lifetime
- 1999-10-01 JP JP2000574666A patent/JP4455764B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1117771A1 (en) | 2001-07-25 |
| CN1320161A (zh) | 2001-10-31 |
| AU5850599A (en) | 2000-04-26 |
| DE69907240D1 (de) | 2003-05-28 |
| DK1117771T3 (da) | 2003-06-16 |
| AU761363B2 (en) | 2003-06-05 |
| JP4455764B2 (ja) | 2010-04-21 |
| CN1197963C (zh) | 2005-04-20 |
| WO2000020569A1 (en) | 2000-04-13 |
| CA2341484C (en) | 2009-08-04 |
| ATE238415T1 (de) | 2003-05-15 |
| CA2341484A1 (en) | 2000-04-13 |
| BR9914217A (pt) | 2001-07-03 |
| JP2002526106A (ja) | 2002-08-20 |
| EP1117771B1 (en) | 2003-04-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69907240T2 (de) | Feste phytase-zusammensetzungen | |
| US8846361B2 (en) | Solid phytase compositions stabilized with corn steep liquor | |
| EP1996028B1 (de) | Feste enymformulierungen und verfahren zu deren herstellung | |
| DE69703371T2 (de) | Nährungszusammensetzung aus dem Maisquellwasser und Verfahren zu deren Herstellung | |
| WO1997016076A1 (en) | Feed enzyme preparations | |
| WO1993016175A1 (en) | Stabilized aqueous liquid formulations of phytase | |
| DE102005043323A1 (de) | Phytasehaltiges Enzymgranulat I | |
| US20230180798A1 (en) | Remediation of toxins in biorefinery process streams | |
| DE102006053071A1 (de) | Protein-enthaltender Stoff mit erhöhter Temperaturstabilität | |
| DE2711753A1 (de) | Stabilisierte fluessige coenzympraeparate | |
| EP1931215B1 (de) | L-lysin enthaltende futtermitteladditive | |
| US20030206881A1 (en) | Phospholipidic composition as well as the use thereof | |
| SU1097253A1 (ru) | Способ получени сухого ацидофильного препарата | |
| US20060165674A1 (en) | Granular composition and process for producing the same | |
| EP0106146A1 (de) | Verfahren zur biologischen Herstellung von Glutathion | |
| EP2244584B1 (en) | Process for preparing feed and feed additives of enhanced nutritive value from industrial by-products containing vegetable oils | |
| DE2745035A1 (de) | Als futtermittelzusatz verwendbares zinkbacitracinpraeparat und verfahren zu seiner herstellung | |
| US7566556B2 (en) | Enhancing enzyme thermostability by mixing with sorghum liquor waste | |
| DE2165406C3 (de) | Verfahren zur Isolierung vom anorganischen Phosphat abhängigen Enzymen | |
| DE2745206A1 (de) | Verfahren zur herstellung einer oligosaccharidfraktion | |
| MXPA97005440A (en) | Improved nutritive composition resulting from maize soaking and its obtenc procedure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |