DE69637760D1 - Reinigungsprozess für igf-i - Google Patents

Reinigungsprozess für igf-i

Info

Publication number
DE69637760D1
DE69637760D1 DE69637760T DE69637760T DE69637760D1 DE 69637760 D1 DE69637760 D1 DE 69637760D1 DE 69637760 T DE69637760 T DE 69637760T DE 69637760 T DE69637760 T DE 69637760T DE 69637760 D1 DE69637760 D1 DE 69637760D1
Authority
DE
Germany
Prior art keywords
igf
unfolding
refolding
reverse phase
cation exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69637760T
Other languages
English (en)
Inventor
Russell A Brierley
Joan N Abrams
John M Hanson
Francis C Maslanka
Cynthia Cowgill
Luis G Juarbe-Osorio
Patricio Riquelme
Glenn Dorin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Cephalon LLC
Original Assignee
Novartis Vaccines and Diagnostics Inc
Cephalon LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Vaccines and Diagnostics Inc, Cephalon LLC filed Critical Novartis Vaccines and Diagnostics Inc
Application granted granted Critical
Publication of DE69637760D1 publication Critical patent/DE69637760D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
DE69637760T 1995-04-14 1996-04-12 Reinigungsprozess für igf-i Expired - Lifetime DE69637760D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/422,436 US5650496A (en) 1995-04-14 1995-04-14 IGF-I purification process
PCT/US1996/005099 WO1996032407A1 (en) 1995-04-14 1996-04-12 Igf-i purification process

Publications (1)

Publication Number Publication Date
DE69637760D1 true DE69637760D1 (de) 2009-01-08

Family

ID=23674879

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69637760T Expired - Lifetime DE69637760D1 (de) 1995-04-14 1996-04-12 Reinigungsprozess für igf-i

Country Status (11)

Country Link
US (2) US5650496A (de)
EP (1) EP0820463B1 (de)
JP (1) JP3894570B2 (de)
AT (1) ATE415411T1 (de)
AU (2) AU696516B2 (de)
CA (1) CA2218111C (de)
DE (1) DE69637760D1 (de)
ES (1) ES2321242T3 (de)
NO (1) NO322326B1 (de)
NZ (1) NZ306782A (de)
WO (1) WO1996032407A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3545795A (en) * 1994-09-08 1996-03-27 Chiron Corporation A method of improved production of insulin-like growth factor
US6756484B1 (en) 1995-04-14 2004-06-29 Cephalon, Inc. IGF-I purification process
US7071313B1 (en) * 1995-06-07 2006-07-04 Cephalon, Inc. Methods of purifying authentic IGF from yeast hosts
US7193042B1 (en) 1995-06-07 2007-03-20 Chiron Corporation Methods for purifying authentic IGF from yeast hosts
ATE253079T1 (de) * 1995-06-07 2003-11-15 Chiron Corp Methoden zur aufreinigung von authentischem igf aus hefewirtszellen
BR9713055A (pt) * 1996-11-15 2000-04-04 Genentech Inc Processo para isolar uma neurotrofina humana recombinante, composição de neurotrofina e processo para purificar uma neurotrofina
JP2003530316A (ja) 1999-12-22 2003-10-14 ノボ ノルディスク アクティーゼルスカブ スクランブルした一本鎖ポリペプチドの抽出的再生法
GB0011278D0 (en) 2000-05-10 2000-06-28 Univ London Repair of nerve damage
ATE442041T1 (de) * 2000-07-28 2009-09-15 Murphy Christopher J Medium für transplantat
WO2003031471A1 (en) * 2001-10-09 2003-04-17 The Government Of The United States Of America, As Represented By The Department Of Health And Human Services Methods and compositions for production and purification of recombinant staphylococcal enterotoxin b (rseb)
KR20030044696A (ko) * 2001-11-30 2003-06-09 정종문 피키아 파스토리스에서 재조합 인슐린 유사 성장인자-1단백질을 연속적으로 생산하는 방법
GB0202906D0 (en) 2002-02-07 2002-03-27 Univ London Prevention of myocardial damage
US7229554B2 (en) * 2002-09-25 2007-06-12 Novo Nordisk A/S Purification process comprising microfiltration at elevated temperatures
US20070207209A1 (en) * 2004-08-27 2007-09-06 Murphy Christopher J Trophic factor combinations for nervous system treatment
JP2008533114A (ja) * 2005-03-18 2008-08-21 ユーシーエル ビジネス パブリック リミテッド カンパニー メカノ成長因子ペプチドおよびその使用
KR102178191B1 (ko) 2008-09-02 2020-11-13 머크 밀리포어 리미티드 크로마토그래피 막, 이를 포함하는 장치 및 이의 이용방법
RU2526391C2 (ru) * 2009-02-19 2014-08-20 Кселлия Фармасьютикалз Апс Способ очистки липопептидов
KR20120093349A (ko) * 2009-11-13 2012-08-22 나트릭스 세퍼레이션즈, 인코포레이티드 소수성 상호반응 크로마토그래피용 막 및 그의 사용방법
CA2836460C (en) 2011-05-17 2021-09-21 Natrix Separations Inc. Methods of using a fluid treatment device
SG11201905510XA (en) * 2017-01-30 2019-08-27 Regeneron Pharma Compositions and methods for reducing bioburden in chromatography
WO2022097216A1 (ja) * 2020-11-05 2022-05-12 シミックホールディングス株式会社 組み換えインスリン様成長因子ｉの製造方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5093317A (en) * 1989-06-05 1992-03-03 Cephalon, Inc. Treating disorders by application of insulin-like growth factor
ATE154073T1 (de) * 1990-08-20 1997-06-15 Novo Nordisk As Prozess für die herstellung von biologisch aktivem igf-1 durch verwendung von amino-terminal verlängertem igf-1
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5231178A (en) * 1991-01-16 1993-07-27 The Salk Institute Biotechnology/Industrial Associates, Inc. Method for the purification of intact, correctly-folded insulin-like growth factor-1
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
EP0586667A1 (de) * 1992-03-24 1994-03-16 Synergen, Inc. Rückfaltung und Reinigung von insulinähnlichen Wachstumsfaktor I
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
US5446024A (en) * 1993-12-17 1995-08-29 Genentech, Inc. Purification of insulin-like growth factor

Also Published As

Publication number Publication date
EP0820463A1 (de) 1998-01-28
NO974740L (no) 1997-12-12
AU5543396A (en) 1996-10-30
JP3894570B2 (ja) 2007-03-22
EP0820463B1 (de) 2008-11-26
US5650496A (en) 1997-07-22
AU2007201259A1 (en) 2007-04-05
AU696516B2 (en) 1998-09-10
CA2218111A1 (en) 1996-10-17
JPH11503733A (ja) 1999-03-30
WO1996032407A1 (en) 1996-10-17
CA2218111C (en) 2008-07-08
ATE415411T1 (de) 2008-12-15
NZ306782A (en) 1998-11-25
NO974740D0 (no) 1997-10-13
US6207806B1 (en) 2001-03-27
ES2321242T3 (es) 2009-06-03
EP0820463A4 (de) 2001-08-08
NO322326B1 (no) 2006-09-18

Similar Documents

Publication Publication Date Title
DE69637760D1 (de) Reinigungsprozess für igf-i
ATE241012T1 (de) Verfahren zur herstellung von humanen relaxin
AU6860396A (en) Purified coprinus laccases and nucleic acids encoding same
DE69716767D1 (de) Verfahren zur herstellung von tolterodin
FI922105A (fi) Foerfarande foer isolering av faktor viii.
CA2170515A1 (en) Nucleotide sequences for novel protein tyrosine phosphatases
DK0779896T3 (da) Ny fremgangsmåde til fremstilling af biologisk aktivt protein (TGF)
WO1996011951A3 (en) Keratinocyte growth factor analogs
DK0775159T3 (da) Ny fremgangsmåde til fremstilling af biologisk aktivt dimert protein
ATE128447T1 (de) Verfahren zur herstellung von äthanderivaten.
DK0935647T3 (da) Koncentrerede enfasede vandige opløsninger af tetrahydroiso-alfa-syrer og fremgangsmåder til fremstilling af samme
DE69603530T2 (de) Verfahren zur Herstellung von 1,1-Difluorethan
AU5024490A (en) Method of recovering purified epi protein from a solution especially a fermentation solution
ATE186737T1 (de) Ein verbessertes verfahren für die herstellung von seitenketten-derivaten von cyclohexapeptidyl- lipopeptiden
EP1020421A4 (de) Verfahren zur herstellung von 1,2-propandiol
FR2730729B1 (fr) Procede de coproduction de difluoromethane et de 1,1,1,2-tetrafluoroethane
RU94017615A (ru) Способ получения пептида
KR960010862A (ko) 재조합 인간 성장 호르몬의 개선된 정제 방법

Legal Events

Date Code Title Description
8364 No opposition during term of opposition