DE69631149D1 - Formulierungen in form von emulsionen zur verabreichung von nukleinsäuren an zellen - Google Patents

Formulierungen in form von emulsionen zur verabreichung von nukleinsäuren an zellen

Info

Publication number
DE69631149D1
DE69631149D1 DE69631149T DE69631149T DE69631149D1 DE 69631149 D1 DE69631149 D1 DE 69631149D1 DE 69631149 T DE69631149 T DE 69631149T DE 69631149 T DE69631149 T DE 69631149T DE 69631149 D1 DE69631149 D1 DE 69631149D1
Authority
DE
Germany
Prior art keywords
formulations
emulsions
administration
cells
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69631149T
Other languages
English (en)
Other versions
DE69631149T2 (de
Inventor
Dexi Liu
Feng Liu
Jing-Ping Yang
Leaf Huang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pittsburgh
Original Assignee
University of Pittsburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pittsburgh filed Critical University of Pittsburgh
Application granted granted Critical
Publication of DE69631149D1 publication Critical patent/DE69631149D1/de
Publication of DE69631149T2 publication Critical patent/DE69631149T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/10Dispersions; Emulsions
    • A61K9/107Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/1075Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Epidemiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69631149T 1995-09-26 1996-09-26 Formulierungen in form von emulsionen zur verabreichung von nukleinsäuren an zellen Expired - Fee Related DE69631149T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US534180 1995-09-26
US08/534,180 US6120794A (en) 1995-09-26 1995-09-26 Emulsion and micellar formulations for the delivery of biologically active substances to cells
PCT/US1996/015388 WO1997011682A2 (en) 1995-09-26 1996-09-26 Emulsion and micellar formulations for the delivery of biologically active substances to cells

Publications (2)

Publication Number Publication Date
DE69631149D1 true DE69631149D1 (de) 2004-01-29
DE69631149T2 DE69631149T2 (de) 2004-09-02

Family

ID=24129008

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69631149T Expired - Fee Related DE69631149T2 (de) 1995-09-26 1996-09-26 Formulierungen in form von emulsionen zur verabreichung von nukleinsäuren an zellen

Country Status (10)

Country Link
US (3) US6120794A (de)
EP (1) EP0852490B1 (de)
JP (1) JPH11512712A (de)
AT (1) ATE256452T1 (de)
CA (1) CA2230940A1 (de)
DE (1) DE69631149T2 (de)
DK (1) DK0852490T3 (de)
ES (1) ES2208763T3 (de)
PT (1) PT852490E (de)
WO (1) WO1997011682A2 (de)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6509032B1 (en) * 1991-08-28 2003-01-21 Mcmaster University Cationic amphiphiles
US5855911A (en) * 1995-08-29 1999-01-05 Board Of Regents, The University Of Texas System Liposomal phosphodiester, phosphorothioate, and P-ethoxy oligonucleotides
US6120794A (en) 1995-09-26 2000-09-19 University Of Pittsburgh Emulsion and micellar formulations for the delivery of biologically active substances to cells
US5789244A (en) 1996-01-08 1998-08-04 Canji, Inc. Compositions and methods for the treatment of cancer using recombinant viral vector delivery systems
US6392069B2 (en) 1996-01-08 2002-05-21 Canji, Inc. Compositions for enhancing delivery of nucleic acids to cells
US6258792B1 (en) 1996-04-12 2001-07-10 University Of Pittsburgh Cationic cholesteryl derivatives containing cyclic polar groups
ES2165050T3 (es) 1996-04-12 2002-03-01 Univ Pittsburgh Nuevos derivados cationicos de colesterilo que contienen grupos polares ciclicos.
US6610321B2 (en) 1996-07-03 2003-08-26 University Of Pittsburgh Emulsion formulations for hydrophilic active agents
US7309692B1 (en) * 1996-07-08 2007-12-18 Board Of Regents, The University Of Texas System Inhibition of chronic myelogenous leukemic cell growth by liposomal-antisense oligodeoxy-nucleotides targeting to GRB2 or CRK1
WO1998006437A2 (en) 1996-08-13 1998-02-19 Chiron Corporation Compositions and methods for polynucleotide delivery
US6977244B2 (en) * 1996-10-04 2005-12-20 Board Of Regents, The University Of Texas Systems Inhibition of Bcl-2 protein expression by liposomal antisense oligodeoxynucleotides
US6458373B1 (en) 1997-01-07 2002-10-01 Sonus Pharmaceuticals, Inc. Emulsion vehicle for poorly soluble drugs
US6884430B1 (en) 1997-02-10 2005-04-26 Aventis Pharma S.A. Formulation of stabilized cationic transfection agent(s) /nucleic acid particles
FR2759298B1 (fr) * 1997-02-10 1999-04-09 Rhone Poulenc Rorer Sa Formulation de particules agent(s) de transfection cationique(s)/acides nucleiques stabilisees
US6197332B1 (en) 1997-08-13 2001-03-06 Chiron Corporation Lipid-conjugated polyamide compounds and related compositions and methods thereof
US7285288B1 (en) 1997-10-03 2007-10-23 Board Of Regents, The University Of Texas System Inhibition of Bcl-2 protein expression by liposomal antisense oligodeoxynucleotides
US7704962B1 (en) 1997-10-03 2010-04-27 Board Of Regents, The University Of Texas System Small oligonucleotides with anti-tumor activity
AU1198599A (en) * 1997-10-24 1999-05-17 Neorx Corporation Delivery vehicles for bioactive agents and uses thereof
GB9726073D0 (en) * 1997-12-09 1998-02-04 Smithkline Beecham Plc Novel compounds
US20010003580A1 (en) * 1998-01-14 2001-06-14 Poh K. Hui Preparation of a lipid blend and a phospholipid suspension containing the lipid blend
US7030155B2 (en) 1998-06-05 2006-04-18 Sonus Pharmaceuticals, Inc. Emulsion vehicle for poorly soluble drugs
US6770282B1 (en) * 1998-10-23 2004-08-03 Heska Corporation Cationic lipid-mediated enhancement of nucleic acid immunization of cats
CA2407074A1 (en) * 2000-04-21 2001-11-01 Jukka Hartikka Compositions and methods for in vivo delivery of polynucleotide-based therapeutics
AU7542301A (en) * 2000-06-09 2001-12-17 Teni Boulikas Encapsulation of plasmid DNA (lipogenes<sup>TM</sup>) and therapeutic agents with nuclear localization signal/fusogenic peptide conjugates into targeted liposome complexes
FR2814958B1 (fr) * 2000-10-06 2003-03-07 Aventis Pasteur Composition vaccinale
US6676963B1 (en) * 2000-10-27 2004-01-13 Barnes-Jewish Hospital Ligand-targeted emulsions carrying bioactive agents
US20050227911A1 (en) * 2001-09-28 2005-10-13 Solubest Ltd. Hydrophilic dispersions of nanoparticles of inclusion complexes of macromolecules
US6878693B2 (en) * 2001-09-28 2005-04-12 Solubest Ltd. Hydrophilic complexes of lipophilic materials and an apparatus and method for their production
US20050233003A1 (en) * 2001-09-28 2005-10-20 Solubest Ltd. Hydrophilic dispersions of nanoparticles of inclusion complexes of salicylic acid
US7700851B2 (en) * 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
WO2003061386A1 (en) * 2002-01-03 2003-07-31 Board Of Regents, The University Of Texas System Wt1 antisense oligos for the inhibition of breast cancer
ATE350638T1 (de) 2002-01-17 2007-01-15 York Refrigeration Aps Getauchter verdampfer mit integriertem wärmeaustauscher
JP2006500420A (ja) * 2002-09-26 2006-01-05 ファイザー・プロダクツ・インク ブタ筋細胞によるdna取込みを増大させるための賦形剤の使用
US20040115226A1 (en) * 2002-12-12 2004-06-17 Wenji Li Free-flowing solid formulations with improved bio-availability of poorly water soluble drugs and process for making the same
US7772188B2 (en) 2003-01-28 2010-08-10 Ironwood Pharmaceuticals, Inc. Methods and compositions for the treatment of gastrointestinal disorders
US8802116B2 (en) * 2003-02-27 2014-08-12 Novasel Australia Pty. Ltd. Poloxamer emulsion preparations
US20050095283A1 (en) * 2003-09-16 2005-05-05 Aphios Corporation Compositions and methods for topically treating diseases
GB0401513D0 (en) 2004-01-23 2004-02-25 Camurus Ab Compositions
US20050239747A1 (en) * 2004-04-21 2005-10-27 Pharmaceutical Industry Technology And Development Center Compositions and methods of enhanced transdermal delivery of steroid compounds and preparation methods
WO2005105040A2 (en) * 2004-04-26 2005-11-10 Micelle Products, Inc. Water-soluble formulations of fat soluble vitamins and pharmaceutical agents and their applications
US20060134221A1 (en) * 2004-12-03 2006-06-22 Vical Incorporated Methods for producing block copolymer/amphiphilic particles
US20120269886A1 (en) 2004-12-22 2012-10-25 Nitto Denko Corporation Therapeutic agent for pulmonary fibrosis
JP2009221164A (ja) 2008-03-17 2009-10-01 Nitto Denko Corp 肺線維症処置剤
JP4121537B2 (ja) 2004-12-22 2008-07-23 日東電工株式会社 線維化抑制のための薬物担体および薬物担体キット
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
TWI407971B (zh) 2007-03-30 2013-09-11 Nitto Denko Corp Cancer cells and tumor-related fibroblasts
NZ582586A (en) 2007-07-21 2011-12-22 Albany Molecular Res Inc 5-pyridinone substituted indazoles
CN102026970B (zh) 2007-11-21 2013-07-31 解码遗传Ehf公司 用于治疗肺部和心血管病症的联芳基pde4抑制剂
EP2240481A1 (de) 2008-01-11 2010-10-20 Albany Molecular Research, Inc. (1-azinon)-substituierte pyridoindole als mch-antagonisten
WO2009142893A2 (en) * 2008-05-19 2009-11-26 The University Of North Carolina At Chapel Hill Methods and compositions for the delivery of bioactive compounds
US8900627B2 (en) * 2008-06-06 2014-12-02 Mirna Therapeutics, Inc. Compositions for the in vivo delivery of RNAi agents
KR20110051214A (ko) * 2008-07-30 2011-05-17 닛토덴코 가부시키가이샤 약물 담체
US9445975B2 (en) 2008-10-03 2016-09-20 Access Business Group International, Llc Composition and method for preparing stable unilamellar liposomal suspension
WO2010059836A1 (en) 2008-11-20 2010-05-27 Decode Genetics Ehf Substituted aza-bridged bicyclics for cardiovascular and cns disease
EP2389383B1 (de) 2009-01-26 2019-04-24 Israel Institute For Biological Research Bicyclische heterocyclische spiroverbindungen
US8040068B2 (en) 2009-02-05 2011-10-18 Mks Instruments, Inc. Radio frequency power control system
WO2011008572A2 (en) 2009-07-14 2011-01-20 Albany Molecular Research, Inc. 5-ht3 receptor modulators, methods of making, and use thereof
RU2625546C2 (ru) 2010-07-06 2017-07-14 Новартис Аг Катионные эмульсии "масло-в-воде"
JP5950428B2 (ja) 2010-08-05 2016-07-13 日東電工株式会社 線維化組織から正常組織を再生するための組成物
JP6120839B2 (ja) 2011-07-06 2017-04-26 ノバルティス アーゲー カチオン性水中油型エマルジョン
WO2013006834A1 (en) 2011-07-06 2013-01-10 Novartis Ag Oil-in-water emulsions that contain nucleic acids
US20130243867A1 (en) * 2012-02-23 2013-09-19 University Of South Florida (A Florida Non-Profit Corporation) Micelle compositions and methods for their use
JP6340162B2 (ja) 2012-12-20 2018-06-06 日東電工株式会社 アポトーシス誘導剤
ES2821966T3 (es) 2014-04-02 2021-04-28 Nitto Denko Corp Molécula de direccionamiento derivada de RBP y utilización de la misma
KR102256453B1 (ko) 2014-04-07 2021-05-25 닛토덴코 가부시키가이샤 소수성 약물 전달용 신규한 폴리머계 하이드로트로프
EA201791437A1 (ru) 2014-12-31 2017-12-29 Лантеус Медикал Имэджинг, Инк. Композиции микросфер c инкапсулированным в липиде газом и соответствующие способы
TWI740937B (zh) 2016-05-04 2021-10-01 美商藍瑟斯醫學影像公司 用於製備超音波顯影劑之裝置及方法
US9789210B1 (en) 2016-07-06 2017-10-17 Lantheus Medical Imaging, Inc. Methods for making ultrasound contrast agents
CA3093340A1 (en) 2018-03-20 2019-09-26 Icahn School Of Medicine At Mount Sinai Kinase inhibitor compounds and compositions and methods of use
AU2019419414A1 (en) 2018-12-31 2023-04-06 Icahn School Of Medicine At Mount Sinai Kinase inhibitor compounds and compositions and methods of use

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE387647C (de) * 1922-04-16 1924-01-08 Fritsche & Thein Einfuehrungslsolator
US4358013A (en) * 1980-07-28 1982-11-09 Shebley David P Meditation supporting device
US4544545A (en) * 1983-06-20 1985-10-01 Trustees University Of Massachusetts Liposomes containing modified cholesterol for organ targeting
US5545412A (en) * 1985-01-07 1996-08-13 Syntex (U.S.A.) Inc. N-[1, (1-1)-dialkyloxy]-and N-[1, (1-1)-dialkenyloxy]-alk-1-yl-n,n,n-tetrasubstituted ammonium lipids and uses therefor
US4897355A (en) * 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4610888A (en) 1985-05-17 1986-09-09 Anheuser-Busch Companies, Inc. Beer foam enhancing process and apparatus
US5374548A (en) * 1986-05-02 1994-12-20 Genentech, Inc. Methods and compositions for the attachment of proteins to liposomes using a glycophospholipid anchor
US5540933A (en) * 1985-05-31 1996-07-30 La Jolla Cancer Research Foundation Isolation and use of fibronectin receptor
US5043164A (en) * 1989-01-17 1991-08-27 The University Of Tennessee Research Corporation Blood-stable, cholesterol-free liposomes
DE3908047A1 (de) * 1989-03-13 1990-09-20 Desitin Arzneimittel Gmbh Hochdisperse pharmazeutische zusammensetzung
US4958013A (en) * 1989-06-06 1990-09-18 Northwestern University Cholesteryl modified oligonucleotides
US5100662A (en) * 1989-08-23 1992-03-31 The Liposome Company, Inc. Steroidal liposomes exhibiting enhanced stability
US5286634A (en) * 1989-09-28 1994-02-15 Stadler Joan K Synergistic method for host cell transformation
US5676954A (en) 1989-11-03 1997-10-14 Vanderbilt University Method of in vivo delivery of functioning foreign genes
US5279833A (en) * 1990-04-04 1994-01-18 Yale University Liposomal transfection of nucleic acids into animal cells
US5264618A (en) * 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
EP0599850B1 (de) 1991-08-16 1996-01-10 Vical, Inc. Zusammensetzung und verfahren zur behandlung von zystischer fibrose
US5283185A (en) * 1991-08-28 1994-02-01 University Of Tennessee Research Corporation Method for delivering nucleic acids into cells
US5756353A (en) 1991-12-17 1998-05-26 The Regents Of The University Of California Expression of cloned genes in the lung by aerosol-and liposome-based delivery
IL101241A (en) * 1992-03-16 1997-11-20 Yissum Res Dev Co Pharmaceutical or cosmetic composition comprising stabilized oil-in-water type emulsion as carrier
US6113946A (en) * 1992-04-03 2000-09-05 The Regents Of The University Of California Self-assembling polynucleotide delivery system comprising dendrimer polycations
JP3285355B2 (ja) 1992-06-04 2002-05-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア invivo遺伝子治療のための方法及び組成物
US5334761A (en) * 1992-08-28 1994-08-02 Life Technologies, Inc. Cationic lipids
JP2854203B2 (ja) * 1992-09-03 1999-02-03 株式会社ディ・ディ・エス研究所 リポソームの製造法
AU6254494A (en) * 1993-02-16 1994-09-14 Virginia Tech Intellectual Properties, Inc. Polyelectrolyte dna conjugation and genetic transformation of an animal
WO1995002698A1 (en) * 1993-07-12 1995-01-26 Life Technologies, Inc. Composition and methods for transfecting eukaryotic cells
IL107471A (en) * 1993-11-02 1997-09-30 Yissum Res Dev Co Pharmaceutical preparation comprising a self- emulsifying formulation and process for its production
US6077835A (en) 1994-03-23 2000-06-20 Case Western Reserve University Delivery of compacted nucleic acid to cells
US5844107A (en) * 1994-03-23 1998-12-01 Case Western Reserve University Compacted nucleic acids and their delivery to cells
AU696455C (en) 1994-03-23 2006-03-02 Case Western Reserve University Compacted nucleic acids and their delivery to cells
US5972901A (en) * 1994-03-23 1999-10-26 Case Western Reserve University Serpin enzyme complex receptor--mediated gene transfer
US5885613A (en) * 1994-09-30 1999-03-23 The University Of British Columbia Bilayer stabilizing components and their use in forming programmable fusogenic liposomes
US5635487A (en) * 1994-12-29 1997-06-03 Wolff; Jon A. Amphipathic, micellar delivery systems for biologically active polyions
US6008202A (en) * 1995-01-23 1999-12-28 University Of Pittsburgh Stable lipid-comprising drug delivery complexes and methods for their production
US5795587A (en) * 1995-01-23 1998-08-18 University Of Pittsburgh Stable lipid-comprising drug delivery complexes and methods for their production
US5705385A (en) * 1995-06-07 1998-01-06 Inex Pharmaceuticals Corporation Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer
US6120794A (en) 1995-09-26 2000-09-19 University Of Pittsburgh Emulsion and micellar formulations for the delivery of biologically active substances to cells
US6270795B1 (en) 1995-11-09 2001-08-07 Microbiological Research Authority Method of making microencapsulated DNA for vaccination and gene therapy
ES2165050T3 (es) 1996-04-12 2002-03-01 Univ Pittsburgh Nuevos derivados cationicos de colesterilo que contienen grupos polares ciclicos.
US6610321B2 (en) 1996-07-03 2003-08-26 University Of Pittsburgh Emulsion formulations for hydrophilic active agents
WO1998008489A1 (en) 1996-08-26 1998-03-05 Transgene S.A. Cationic lipid-nucleic acid complexes
US6271209B1 (en) 1998-04-03 2001-08-07 Valentis, Inc. Cationic lipid formulation delivering nucleic acid to peritoneal tumors

Also Published As

Publication number Publication date
DE69631149T2 (de) 2004-09-02
EP0852490A2 (de) 1998-07-15
ATE256452T1 (de) 2004-01-15
ES2208763T3 (es) 2004-06-16
JPH11512712A (ja) 1999-11-02
US20030211143A1 (en) 2003-11-13
US20020090377A1 (en) 2002-07-11
US6586003B2 (en) 2003-07-01
EP0852490B1 (de) 2003-12-17
PT852490E (pt) 2004-04-30
CA2230940A1 (en) 1997-04-03
US6120794A (en) 2000-09-19
DK0852490T3 (da) 2004-04-19
WO1997011682A2 (en) 1997-04-03
WO1997011682A3 (en) 1997-07-10

Similar Documents

Publication Publication Date Title
DE69631149D1 (de) Formulierungen in form von emulsionen zur verabreichung von nukleinsäuren an zellen
Kotla et al. Biomimetic lipid-based nanosystems for enhanced dermal delivery of drugs and bioactive agents
Badea et al. In vivo cutaneous interferon‐γ gene delivery using novel dicationic (gemini) surfactant–plasmid complexes
Wareing et al. Cationic liposome mediated transgene expression in the guinea pig cochlea
AU728581B2 (en) Liposomes
Dujardin et al. Topical gene transfer into rat skin using electroporation
Faneca et al. Evaluation of lipid-based reagents to mediate intracellular gene delivery
Mizuguchi et al. Efficient gene transfer into mammalian cells using fusogenic liposome
DE69736385T2 (de) Kationisches lipid und rezeptorligand erleichterte gabe von biologisch aktiven molekuelen
ATE172636T1 (de) Liposomales verabreichungssystem für biologisch aktive stoffe
Garbuzenko et al. Electrostatics of PEGylated micelles and liposomes containing charged and neutral lipopolymers
Balbino et al. Correlation of the physicochemical and structural properties of pDNA/cationic liposome complexes with their in vitro transfection
Sato et al. Neutralization of negative charges of siRNA results in improved safety and efficient gene silencing activity of lipid nanoparticles loaded with high levels of siRNA
PT799059E (pt) Anfifilos cationicos e plasmideos para administracao intracelular de moleculas terapeuticas
KR20060028655A (ko) 지질 캡슐화된 간섭 rna
An et al. Recent advances in the transdermal delivery of protein therapeutics with a combinatorial system of chemical adjuvants and physical penetration enhancements
Ceccoli et al. Encapsulation of the UV-DNA repair enzyme T4 endonuclease V in liposomes and delivery to human cells
US5283122A (en) Fused liposome and acid induced method for liposome fusion
Bolsoni et al. Lipid nanoparticle-mediated hit-and-run approaches yield efficient and safe in situ gene editing in human skin
US6316260B1 (en) Tetraether lipid derivatives and liposomes and lipid agglomerates containing tetraether lipid derivatives, and use thereof
Hug et al. Fusogenic virosomes prepared by partitioning of vesicular stomatitis virus G protein into preformed vesicles.
Mignet et al. Cationic and anionic lipoplexes inhibit gene transfection by electroporation in vivo
Meyer et al. Multiple systemic expression of human interferon-β in mice can be achieved upon repeated administration of optimized pcTG90-lipoplex
Konopka et al. Cationic liposome-mediated expression of HIV-regulated luciferase and diphtheria toxin a genes in HeLa cells infected with or expressing HIV
Daniels et al. Pegylated and Non-Pegylated siRNA lipoplexes formulated with cholesteryl cytofectins promote efficient Luciferase knockdown in HeLa tat luc cells

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee