DE69613228T2 - Extraktion von triglyceriden aus mikroorganismen - Google Patents
Extraktion von triglyceriden aus mikroorganismenInfo
- Publication number
- DE69613228T2 DE69613228T2 DE69613228T DE69613228T DE69613228T2 DE 69613228 T2 DE69613228 T2 DE 69613228T2 DE 69613228 T DE69613228 T DE 69613228T DE 69613228 T DE69613228 T DE 69613228T DE 69613228 T2 DE69613228 T2 DE 69613228T2
- Authority
- DE
- Germany
- Prior art keywords
- solvent
- oil
- microorganisms
- comminuted
- process according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 33
- 238000000605 extraction Methods 0.000 title claims description 18
- 150000003626 triacylglycerols Chemical class 0.000 title description 4
- 239000002904 solvent Substances 0.000 claims abstract description 52
- 239000000463 material Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000001413 cellular effect Effects 0.000 claims abstract description 4
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 51
- 238000000034 method Methods 0.000 claims description 25
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 20
- 210000004027 cell Anatomy 0.000 claims description 18
- 210000002421 cell wall Anatomy 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 239000004215 Carbon black (E152) Substances 0.000 claims description 3
- 241000907999 Mortierella alpina Species 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 238000000265 homogenisation Methods 0.000 claims description 3
- 229930195733 hydrocarbon Natural products 0.000 claims description 3
- 150000002430 hydrocarbons Chemical group 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 230000002934 lysing effect Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 2
- 239000003921 oil Substances 0.000 description 29
- 235000019198 oils Nutrition 0.000 description 29
- 239000012071 phase Substances 0.000 description 25
- 239000008346 aqueous phase Substances 0.000 description 13
- 230000000813 microbial effect Effects 0.000 description 11
- YZXBAPSDXZZRGB-UHFFFAOYSA-N icosa-5,8,11,14-tetraenoic acid Chemical compound CCCCCC=CCC=CCC=CCC=CCCCC(O)=O YZXBAPSDXZZRGB-UHFFFAOYSA-N 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000000839 emulsion Substances 0.000 description 4
- 230000005484 gravity Effects 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 239000012736 aqueous medium Substances 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- MBMBGCFOFBJSGT-KUBAVDMBSA-N docosahexaenoic acid Natural products CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- YEBDWAHEIMUJQT-XVSDJDOKSA-N CCCCCC=CCC=CCC=CCC=CCCCC(O)=O.CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O Chemical compound CCCCCC=CCC=CCC=CCC=CCCCC(O)=O.CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YEBDWAHEIMUJQT-XVSDJDOKSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 description 2
- 239000002518 antifoaming agent Substances 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 239000008158 vegetable oil Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
- 239000007832 Na2SO4 Substances 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 241000233675 Thraustochytrium Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000000498 ball milling Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- MBMBGCFOFBJSGT-SFGLVEFQSA-N docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C\C\C=C\C\C=C\C\C=C\C\C=C\C\C=C\CCC(O)=O MBMBGCFOFBJSGT-SFGLVEFQSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000002035 hexane extract Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 239000012454 non-polar solvent Substances 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 239000003799 water insoluble solvent Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Mycology (AREA)
- Fats And Perfumes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9514649.4A GB9514649D0 (en) | 1995-07-18 | 1995-07-18 | Extraction of triglycerides from microorganisms |
| PCT/GB1996/001703 WO1997004121A1 (en) | 1995-07-18 | 1996-07-16 | Extraction of triglycerides from microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69613228D1 DE69613228D1 (de) | 2001-07-12 |
| DE69613228T2 true DE69613228T2 (de) | 2001-10-11 |
Family
ID=10777825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69613228T Expired - Lifetime DE69613228T2 (de) | 1995-07-18 | 1996-07-16 | Extraktion von triglyceriden aus mikroorganismen |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6180376B1 (enExample) |
| EP (1) | EP0843733B1 (enExample) |
| JP (1) | JP2001507379A (enExample) |
| AT (1) | ATE201907T1 (enExample) |
| AU (1) | AU6467096A (enExample) |
| CA (1) | CA2226427C (enExample) |
| DE (1) | DE69613228T2 (enExample) |
| DK (1) | DK0843733T3 (enExample) |
| ES (1) | ES2158323T3 (enExample) |
| GB (1) | GB9514649D0 (enExample) |
| WO (1) | WO1997004121A1 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2295595B1 (en) | 2000-01-19 | 2019-05-01 | DSM IP Assets B.V. | Solventless extraction process |
| EP1178118A1 (en) * | 2000-08-02 | 2002-02-06 | Dsm N.V. | Isolation of microbial oils |
| TWI240729B (en) | 2000-11-24 | 2005-10-01 | Dow Corning | Process for making silicone emulsions |
| CN104745486B (zh) | 2005-06-07 | 2018-01-16 | 帝斯曼营养品股份公司 | 生产脂质和抗氧化剂的真核微生物 |
| ES2369043T3 (es) | 2005-06-23 | 2011-11-24 | Dsm Ip Assets B.V. | Proceso para obtener un lípido a partir de células. |
| WO2008129358A2 (en) | 2006-08-01 | 2008-10-30 | Ocean Nutrition Canada Ltd. | Oil producing microbes and methods of modification thereof |
| JP2010530741A (ja) * | 2007-06-14 | 2010-09-16 | ミトロプーロス，ニコラオス | バイオ燃料用の藻育成 |
| EP2145942A1 (de) * | 2008-07-15 | 2010-01-20 | Lonza Ltd. | Verfahren zur Isolierung von Ölen aus Zellen und Biomasse |
| US9296985B2 (en) * | 2009-03-10 | 2016-03-29 | Valicor, Inc. | Algae biomass fractionation |
| US8207363B2 (en) * | 2009-03-19 | 2012-06-26 | Martek Biosciences Corporation | Thraustochytrids, fatty acid compositions, and methods of making and uses thereof |
| NZ601757A (en) | 2010-01-19 | 2015-01-30 | Dsm Ip Assets Bv | Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof |
| US8450111B2 (en) | 2010-03-02 | 2013-05-28 | Streamline Automation, Llc | Lipid extraction from microalgae using a single ionic liquid |
| US8303818B2 (en) * | 2010-06-24 | 2012-11-06 | Streamline Automation, Llc | Method and apparatus using an active ionic liquid for algae biofuel harvest and extraction |
| WO2011127127A2 (en) | 2010-04-06 | 2011-10-13 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Extraction with fractionation of oil and co-products from oleaginous material |
| EP2576801B1 (en) | 2010-06-01 | 2019-10-02 | DSM IP Assets B.V. | Extraction of lipid from cells and products therefrom |
| WO2012015831A1 (en) | 2010-07-26 | 2012-02-02 | Sapphire Energy, Inc. | Process for the recovery of oleaginous compounds from biomass |
| US8906236B2 (en) | 2010-07-26 | 2014-12-09 | Sapphire Energy, Inc. | Process for the recovery of oleaginous compounds and nutrients from biomass |
| US9028696B2 (en) | 2010-07-26 | 2015-05-12 | Sapphire Energy, Inc. | Process for the recovery of oleaginous compounds from biomass |
| EP2704999B1 (en) | 2011-05-06 | 2019-02-20 | Ariel-University Research and Development Company, Ltd | Wastewater treatment method comprising algal photosynthesis |
| EP2952183A1 (en) | 2011-07-21 | 2015-12-09 | DSM IP Assets B.V. | Fatty acid compositions |
| US9309544B2 (en) | 2012-04-10 | 2016-04-12 | Kao Corporation | Method for producing fatty acid ester |
| DK2970926T3 (en) | 2013-03-13 | 2018-04-16 | Dsm Nutritional Products Ag | GENMANIPULATION OF MICROorganisms |
| TWI646189B (zh) | 2013-12-20 | 2019-01-01 | 荷蘭商Ｄｓｍ智慧財產有限公司 | 用於從微生物細胞獲得微生物油之方法（五） |
| SG11201605052XA (en) | 2013-12-20 | 2016-07-28 | Dsm Ip Assets Bv | Processes for obtaining microbial oil from microbial cells |
| AU2014369046B2 (en) | 2013-12-20 | 2019-02-28 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| ES2984970T3 (es) | 2013-12-20 | 2024-10-31 | Mara Renewables Corp | Métodos para recuperar aceite de microorganismos |
| MX370606B (es) | 2013-12-20 | 2019-12-18 | Dsm Ip Assets Bv | Procedimientos para obtener aceite microbiano a partir de células microbianas. |
| CN105829539B (zh) * | 2013-12-20 | 2023-11-10 | 帝斯曼营养品股份公司 | 从微生物中回收油的方法 |
| CA2934490C (en) | 2013-12-20 | 2022-05-03 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| US20160002521A1 (en) * | 2014-07-03 | 2016-01-07 | Solazyme, Inc. | Lubricants and wellbore fluids |
| SG11201707876YA (en) | 2015-03-24 | 2017-10-30 | Terravia Holdings Inc | Microalgal compositions and uses thereof |
| AR104042A1 (es) | 2015-03-26 | 2017-06-21 | Mara Renewables Corp | Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto |
| HK1254383A1 (zh) | 2015-07-13 | 2019-07-19 | MARA Renewables Corporation | 增强木糖的微藻代谢 |
| US10851395B2 (en) | 2016-06-10 | 2020-12-01 | MARA Renewables Corporation | Method of making lipids with improved cold flow properties |
| MX2020013907A (es) | 2018-06-29 | 2021-03-09 | Amyris Inc Star | Metodos para recuperar compuestos isoprenoides inmiscibles en agua a partir de biomasa microbiana. |
| CN114207098A (zh) * | 2019-06-14 | 2022-03-18 | 墨尔本大学 | 复杂乳液的剪切诱导的相转化用于从生物质中回收有机成分 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1089093A (en) * | 1963-10-03 | 1967-11-01 | British Petroleum Co | Improvements in or relating to the production of derivatives from micro-organisms |
| IL57712A (en) * | 1979-07-03 | 1984-02-29 | Yissum Res Dev Co | Cultivation of halophilic algae of the dunaliella species for the production of fuel-like product |
| JPS6012036B2 (ja) * | 1982-08-06 | 1985-03-29 | 財団法人工業開発研究所 | ケイソウから油状物質を抽出する方法 |
| WO1986004354A1 (en) * | 1985-01-22 | 1986-07-31 | Japan As Represented By Director General Of Agency | Method for obtaining lipids from fungus bodies |
| US5130242A (en) * | 1988-09-07 | 1992-07-14 | Phycotech, Inc. | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
| PH11992043811B1 (en) * | 1991-01-24 | 2002-08-22 | Martek Corp | Arachidonic acid and methods for the production and use thereof |
| DE69210892T2 (de) | 1991-07-11 | 1996-10-02 | Idemitsu Petrochemical Co | Triglyceride enthaltende trockene Zellfragmente und Verfahren zu deren Herstellung |
-
1995
- 1995-07-18 GB GBGB9514649.4A patent/GB9514649D0/en active Pending
-
1996
- 1996-07-16 EP EP96924096A patent/EP0843733B1/en not_active Expired - Lifetime
- 1996-07-16 ES ES96924096T patent/ES2158323T3/es not_active Expired - Lifetime
- 1996-07-16 US US08/983,250 patent/US6180376B1/en not_active Expired - Lifetime
- 1996-07-16 WO PCT/GB1996/001703 patent/WO1997004121A1/en not_active Ceased
- 1996-07-16 DK DK96924096T patent/DK0843733T3/da active
- 1996-07-16 DE DE69613228T patent/DE69613228T2/de not_active Expired - Lifetime
- 1996-07-16 AU AU64670/96A patent/AU6467096A/en not_active Abandoned
- 1996-07-16 CA CA002226427A patent/CA2226427C/en not_active Expired - Lifetime
- 1996-07-16 JP JP50641897A patent/JP2001507379A/ja not_active Ceased
- 1996-07-16 AT AT96924096T patent/ATE201907T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001507379A (ja) | 2001-06-05 |
| DE69613228D1 (de) | 2001-07-12 |
| CA2226427A1 (en) | 1997-02-06 |
| CA2226427C (en) | 2009-10-13 |
| GB9514649D0 (en) | 1995-09-13 |
| US6180376B1 (en) | 2001-01-30 |
| EP0843733B1 (en) | 2001-06-06 |
| WO1997004121A1 (en) | 1997-02-06 |
| ES2158323T3 (es) | 2001-09-01 |
| DK0843733T3 (da) | 2001-09-03 |
| EP0843733A1 (en) | 1998-05-27 |
| ATE201907T1 (de) | 2001-06-15 |
| AU6467096A (en) | 1997-02-18 |
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