CA2226427C - Extraction of triglycerides from microorganisms - Google Patents

Extraction of triglycerides from microorganisms Download PDF

Info

Publication number
CA2226427C
CA2226427C CA002226427A CA2226427A CA2226427C CA 2226427 C CA2226427 C CA 2226427C CA 002226427 A CA002226427 A CA 002226427A CA 2226427 A CA2226427 A CA 2226427A CA 2226427 C CA2226427 C CA 2226427C
Authority
CA
Canada
Prior art keywords
solvent
microorganisms
disintegrated
triglyceride oil
phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002226427A
Other languages
English (en)
French (fr)
Other versions
CA2226427A1 (en
Inventor
John Macdonald Liddell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of CA2226427A1 publication Critical patent/CA2226427A1/en
Application granted granted Critical
Publication of CA2226427C publication Critical patent/CA2226427C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Sustainable Development (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002226427A 1995-07-18 1996-07-16 Extraction of triglycerides from microorganisms Expired - Lifetime CA2226427C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9514649.4A GB9514649D0 (en) 1995-07-18 1995-07-18 Extraction of triglycerides from microorganisms
GB9514649.4 1995-07-18
PCT/GB1996/001703 WO1997004121A1 (en) 1995-07-18 1996-07-16 Extraction of triglycerides from microorganisms

Publications (2)

Publication Number Publication Date
CA2226427A1 CA2226427A1 (en) 1997-02-06
CA2226427C true CA2226427C (en) 2009-10-13

Family

ID=10777825

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002226427A Expired - Lifetime CA2226427C (en) 1995-07-18 1996-07-16 Extraction of triglycerides from microorganisms

Country Status (11)

Country Link
US (1) US6180376B1 (enExample)
EP (1) EP0843733B1 (enExample)
JP (1) JP2001507379A (enExample)
AT (1) ATE201907T1 (enExample)
AU (1) AU6467096A (enExample)
CA (1) CA2226427C (enExample)
DE (1) DE69613228T2 (enExample)
DK (1) DK0843733T3 (enExample)
ES (1) ES2158323T3 (enExample)
GB (1) GB9514649D0 (enExample)
WO (1) WO1997004121A1 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1252324T3 (da) 2000-01-19 2010-12-20 Martek Biosciences Corp Solventfri ekstraktionsfremgangsmåde
EP1178118A1 (en) * 2000-08-02 2002-02-06 Dsm N.V. Isolation of microbial oils
TWI240729B (en) 2000-11-24 2005-10-01 Dow Corning Process for making silicone emulsions
EP2468847B1 (en) 2005-06-07 2017-03-29 DSM Nutritional Products AG Eukaryotic microorganisms for producing lipids and antioxidants
EA013637B1 (ru) * 2005-06-23 2010-06-30 ДСМ АйПи АССЕТС Б.В. Способ получения липида из клеток
MX2009001346A (es) 2006-08-01 2009-04-17 Ocean Nutrition Canada Ltd Microbios productores de aceite y metodos de modificacion de los mismos.
WO2008151373A1 (en) * 2007-06-14 2008-12-18 Fuji Fuels Pty Ltd Algae growth for biofuels
EP2145942A1 (de) * 2008-07-15 2010-01-20 Lonza Ltd. Verfahren zur Isolierung von Ölen aus Zellen und Biomasse
US9296985B2 (en) * 2009-03-10 2016-03-29 Valicor, Inc. Algae biomass fractionation
US8207363B2 (en) * 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
AU2010343305B2 (en) 2010-01-19 2015-06-11 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
US8303818B2 (en) * 2010-06-24 2012-11-06 Streamline Automation, Llc Method and apparatus using an active ionic liquid for algae biofuel harvest and extraction
US8450111B2 (en) 2010-03-02 2013-05-28 Streamline Automation, Llc Lipid extraction from microalgae using a single ionic liquid
WO2011127127A2 (en) 2010-04-06 2011-10-13 Arizona Board Of Regents For And On Behalf Of Arizona State University Extraction with fractionation of oil and co-products from oleaginous material
JP5911479B2 (ja) 2010-06-01 2016-04-27 ディーエスエム アイピー アセッツ ビー．ブイ． 細胞からの脂質の抽出およびそれに由来する生産物
US9028696B2 (en) 2010-07-26 2015-05-12 Sapphire Energy, Inc. Process for the recovery of oleaginous compounds from biomass
BR112013002003A2 (pt) 2010-07-26 2019-09-24 Sapphire Energy Inc processo para recuperação de compostos oleaginosos de biomassa
US8906236B2 (en) 2010-07-26 2014-12-09 Sapphire Energy, Inc. Process for the recovery of oleaginous compounds and nutrients from biomass
EP2704999B1 (en) 2011-05-06 2019-02-20 Ariel-University Research and Development Company, Ltd Wastewater treatment method comprising algal photosynthesis
TWI582235B (zh) 2011-07-21 2017-05-11 Ｄｓｍ智慧財產有限公司 生產二十碳五烯酸之微生物、脂肪酸組成物及其製作方法與用途
US9309544B2 (en) 2012-04-10 2016-04-12 Kao Corporation Method for producing fatty acid ester
DK2970926T3 (en) 2013-03-13 2018-04-16 Dsm Nutritional Products Ag GENMANIPULATION OF MICROorganisms
CA2934506C (en) 2013-12-20 2022-05-03 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
NZ721409A (en) 2013-12-20 2022-10-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
EP4223861A3 (en) * 2013-12-20 2023-11-01 Mara Renewables Corporation Methods of recovering oil from microorganisms
BR112016014208B1 (pt) 2013-12-20 2022-09-20 Dsm Nutricional Products Ag Método de extração de lipídeos a partir de uma população de micro-organismos
WO2015095690A2 (en) 2013-12-20 2015-06-25 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
CA2934490C (en) 2013-12-20 2022-05-03 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
JP6705581B2 (ja) 2013-12-20 2020-06-03 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． 微生物細胞から微生物油を入手するための方法
US20160002521A1 (en) * 2014-07-03 2016-01-07 Solazyme, Inc. Lubricants and wellbore fluids
DK3274430T3 (da) 2015-03-24 2022-10-03 Corbion Biotech Inc Mikroalgesammensætninger og deres anvendelser
AR104042A1 (es) 2015-03-26 2017-06-21 Mara Renewables Corp Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto
BR112018000690A2 (pt) 2015-07-13 2018-09-18 MARA Renewables Corporation micro-organismo recombinante, e, métodos para preparar um micro-organismo de metabolização de xilose e para produzir óleo.
US10851395B2 (en) 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
CN112771169B (zh) * 2018-06-29 2024-07-09 阿迈瑞斯公司 从微生物生物质回收水不混溶性类异戊二烯化合物的方法
CN114207098A (zh) * 2019-06-14 2022-03-18 墨尔本大学 复杂乳液的剪切诱导的相转化用于从生物质中回收有机成分

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1089093A (en) 1963-10-03 1967-11-01 British Petroleum Co Improvements in or relating to the production of derivatives from micro-organisms
IL57712A (en) * 1979-07-03 1984-02-29 Yissum Res Dev Co Cultivation of halophilic algae of the dunaliella species for the production of fuel-like product
JPS6012036B2 (ja) 1982-08-06 1985-03-29 財団法人工業開発研究所 ケイソウから油状物質を抽出する方法
DE3587044T2 (de) * 1985-01-22 1993-06-03 Japan As Represented By Direct Verfahren zur herstellung von lipiden aus fungusmassen.
US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
PH11992043811B1 (en) * 1991-01-24 2002-08-22 Martek Corp Arachidonic acid and methods for the production and use thereof
DE69210892T2 (de) 1991-07-11 1996-10-02 Idemitsu Petrochemical Co Triglyceride enthaltende trockene Zellfragmente und Verfahren zu deren Herstellung

Also Published As

Publication number Publication date
ATE201907T1 (de) 2001-06-15
EP0843733B1 (en) 2001-06-06
DK0843733T3 (da) 2001-09-03
CA2226427A1 (en) 1997-02-06
ES2158323T3 (es) 2001-09-01
DE69613228T2 (de) 2001-10-11
EP0843733A1 (en) 1998-05-27
GB9514649D0 (en) 1995-09-13
AU6467096A (en) 1997-02-18
US6180376B1 (en) 2001-01-30
JP2001507379A (ja) 2001-06-05
WO1997004121A1 (en) 1997-02-06
DE69613228D1 (de) 2001-07-12

Similar Documents

Publication Publication Date Title
CA2226427C (en) Extraction of triglycerides from microorganisms
US6166231A (en) Two phase extraction of oil from biomass
JP4346692B2 (ja) 微生物バイオマスからのカロテノイド結晶の単離
EP1305440B1 (en) Isolation of microbial oils
KR100995575B1 (ko) 무용매 추출 방법
EP0036699B1 (en) Extraction of poly-beta-hydroxybutyric acid
US4857329A (en) Process for extracting lipids from mortierella genus fungi
Kapchie et al. Oxidative stability of soybean oil in oleosomes as affected by pH and iron
Drouin et al. Biosurfactants and aqueous two‐phase fermentation
Subroto et al. Enzymatic and chemical synthesis of high mono-and diacylglycerol from palm stearin and olein blend at different type of reactor stirrers
Tuntiwiwattanapun et al. Development and scale-up of aqueous surfactant-assisted extraction of canola oil for use as biodiesel feedstock
Leis et al. Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation
US5097012A (en) Solvent extraction of fatty acid stream with liquid water and elevated temperatures and pressures
Pal et al. Optimization of the primary purification process of extracting sphorolipid from the fermentation broth to achieve a higher yield and purity
Li et al. An efficient and high-water-content enzymatic esterification method for the synthesis of β-sitosterol conjugated linoleate via a sodium citrate-based three-liquid-phase system
Amiri et al. Handling the inhibitory role of product accumulation during sophorolipid fermentation in a bubble column reactor with an in situ foam recovery
Srivastava et al. Physicochemical studies on the water‐yeast cells‐gas‐oil system
EP4570898A1 (en) Method for the recovery of a biocatalytically produced product from an organic fluid stream
RU2841716C2 (ru) Способ извлечения биомасла с улучшенной степенью извлечения масла с использованием процесса охлаждения
Sipponen et al. Integrating the opposites of biofuel production: absorption of short-chain alcohols into oleaginous yeast cells for butanol recovery and wet-extraction of microbial oil
TWI293648B (en) Solventless extraction process
Dean Enzymatic and chemical syntheses and purification of sucrose fatty acid esters
Nguyen et al. Production of biodiesel from activated sludge
BR102020022510A2 (pt) Processo de produção de biossurfactante a partir de óleo de milho de destilaria, uso desse óleo e biossurfactante
Nieh Improved quality of soybean oil from fine soybean flour by solvent extraction

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20160718