JP2001507379A - 微生物からのトリグリセリドの抽出 - Google Patents
微生物からのトリグリセリドの抽出Info
- Publication number
- JP2001507379A JP2001507379A JP50641897A JP50641897A JP2001507379A JP 2001507379 A JP2001507379 A JP 2001507379A JP 50641897 A JP50641897 A JP 50641897A JP 50641897 A JP50641897 A JP 50641897A JP 2001507379 A JP2001507379 A JP 2001507379A
- Authority
- JP
- Japan
- Prior art keywords
- solvent
- oil
- microorganisms
- disrupted
- phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 44
- 238000000605 extraction Methods 0.000 title claims description 19
- 150000003626 triacylglycerols Chemical class 0.000 title claims description 6
- 239000002904 solvent Substances 0.000 claims abstract description 67
- 239000000463 material Substances 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims description 58
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 54
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000002609 medium Substances 0.000 claims description 8
- 239000012736 aqueous medium Substances 0.000 claims description 6
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000004215 Carbon black (E152) Substances 0.000 claims description 5
- 229930195733 hydrocarbon Natural products 0.000 claims description 5
- 150000002430 hydrocarbons Chemical group 0.000 claims description 5
- 238000000265 homogenisation Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 abstract description 3
- 239000003921 oil Substances 0.000 description 41
- 235000019198 oils Nutrition 0.000 description 41
- 239000012071 phase Substances 0.000 description 27
- 210000004027 cell Anatomy 0.000 description 18
- 230000000813 microbial effect Effects 0.000 description 12
- 239000008346 aqueous phase Substances 0.000 description 7
- YZXBAPSDXZZRGB-CGRWFSSPSA-N arachidonic acid Chemical compound CCCCC\C=C\C\C=C\C\C=C\C\C=C\CCCC(O)=O YZXBAPSDXZZRGB-CGRWFSSPSA-N 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 210000002421 cell wall Anatomy 0.000 description 5
- MBMBGCFOFBJSGT-KUBAVDMBSA-N docosahexaenoic acid Natural products CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 230000005484 gravity Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 241000907999 Mortierella alpina Species 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- 239000003995 emulsifying agent Substances 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- YEBDWAHEIMUJQT-XVSDJDOKSA-N CCCCCC=CCC=CCC=CCC=CCCCC(O)=O.CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O Chemical compound CCCCCC=CCC=CCC=CCC=CCCCC(O)=O.CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YEBDWAHEIMUJQT-XVSDJDOKSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- MBMBGCFOFBJSGT-SFGLVEFQSA-N docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C\C\C=C\C\C=C\C\C=C\C\C=C\C\C=C\CCC(O)=O MBMBGCFOFBJSGT-SFGLVEFQSA-N 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 239000008158 vegetable oil Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000233675 Thraustochytrium Species 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 238000010296 bead milling Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013530 defoamer Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000002035 hexane extract Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 239000012454 non-polar solvent Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Fats And Perfumes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9514649.4 | 1995-07-18 | ||
| GBGB9514649.4A GB9514649D0 (en) | 1995-07-18 | 1995-07-18 | Extraction of triglycerides from microorganisms |
| PCT/GB1996/001703 WO1997004121A1 (en) | 1995-07-18 | 1996-07-16 | Extraction of triglycerides from microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2001507379A true JP2001507379A (ja) | 2001-06-05 |
| JP2001507379A5 JP2001507379A5 (enExample) | 2004-08-19 |
Family
ID=10777825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP50641897A Ceased JP2001507379A (ja) | 1995-07-18 | 1996-07-16 | 微生物からのトリグリセリドの抽出 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6180376B1 (enExample) |
| EP (1) | EP0843733B1 (enExample) |
| JP (1) | JP2001507379A (enExample) |
| AT (1) | ATE201907T1 (enExample) |
| AU (1) | AU6467096A (enExample) |
| CA (1) | CA2226427C (enExample) |
| DE (1) | DE69613228T2 (enExample) |
| DK (1) | DK0843733T3 (enExample) |
| ES (1) | ES2158323T3 (enExample) |
| GB (1) | GB9514649D0 (enExample) |
| WO (1) | WO1997004121A1 (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009501003A (ja) * | 2005-06-23 | 2009-01-15 | ディーエスエム アイピー アセッツ ビー.ブイ. | 細胞から脂質を得る方法 |
| JP2010530741A (ja) * | 2007-06-14 | 2010-09-16 | ミトロプーロス,ニコラオス | バイオ燃料用の藻育成 |
| JP2016540516A (ja) * | 2013-12-20 | 2016-12-28 | マラ リニューアブルズ コーポレーション | 微生物から油を回収する方法 |
| JP2021529512A (ja) * | 2018-06-29 | 2021-11-04 | アミリス, インコーポレイテッド | 微生物バイオマスから不水溶性イソプレノイド化合物を回収するための方法 |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2397655C (en) * | 2000-01-19 | 2012-06-05 | Craig M. Ruecker | Solventless extraction process |
| EP1178118A1 (en) * | 2000-08-02 | 2002-02-06 | Dsm N.V. | Isolation of microbial oils |
| TWI240729B (en) | 2000-11-24 | 2005-10-01 | Dow Corning | Process for making silicone emulsions |
| MX338235B (es) | 2005-06-07 | 2016-04-08 | Dsm Nutritional Products Ag | Microorganismos eucarioticos para producir lipidos y antioxidantes. |
| EP2082053B1 (en) | 2006-08-01 | 2016-04-13 | DSM Nutritional Products AG | Process for producing microbial oil comprising polyunsaturated fatty acids |
| EP2145942A1 (de) * | 2008-07-15 | 2010-01-20 | Lonza Ltd. | Verfahren zur Isolierung von Ölen aus Zellen und Biomasse |
| US9296985B2 (en) * | 2009-03-10 | 2016-03-29 | Valicor, Inc. | Algae biomass fractionation |
| US8207363B2 (en) * | 2009-03-19 | 2012-06-26 | Martek Biosciences Corporation | Thraustochytrids, fatty acid compositions, and methods of making and uses thereof |
| CA2993690C (en) | 2010-01-19 | 2022-03-22 | Dsm Ip Assets B.V. | Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof |
| US8450111B2 (en) | 2010-03-02 | 2013-05-28 | Streamline Automation, Llc | Lipid extraction from microalgae using a single ionic liquid |
| US8303818B2 (en) * | 2010-06-24 | 2012-11-06 | Streamline Automation, Llc | Method and apparatus using an active ionic liquid for algae biofuel harvest and extraction |
| WO2011127127A2 (en) | 2010-04-06 | 2011-10-13 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Extraction with fractionation of oil and co-products from oleaginous material |
| EP2576801B1 (en) | 2010-06-01 | 2019-10-02 | DSM IP Assets B.V. | Extraction of lipid from cells and products therefrom |
| CN103080325B (zh) | 2010-07-26 | 2014-08-06 | 蓝宝石能源公司 | 用于从生物质回收油质化合物的方法 |
| US9028696B2 (en) | 2010-07-26 | 2015-05-12 | Sapphire Energy, Inc. | Process for the recovery of oleaginous compounds from biomass |
| US8906236B2 (en) | 2010-07-26 | 2014-12-09 | Sapphire Energy, Inc. | Process for the recovery of oleaginous compounds and nutrients from biomass |
| EP2704999B1 (en) | 2011-05-06 | 2019-02-20 | Ariel-University Research and Development Company, Ltd | Wastewater treatment method comprising algal photosynthesis |
| EA035287B1 (ru) | 2011-07-21 | 2020-05-25 | ДСМ АйПи АССЕТС Б.В. | Микроорганизмы, продуцирующие эйкозапентаеновую кислоту, композиции, содержащие жирные кислоты, и способы их получения и применения |
| WO2013153981A1 (ja) | 2012-04-10 | 2013-10-17 | 花王株式会社 | 脂肪酸エステルの製造方法 |
| EP2970926B1 (en) | 2013-03-13 | 2018-01-31 | DSM Nutritional Products AG | Engineering microorganisms |
| BR112016014208B1 (pt) | 2013-12-20 | 2022-09-20 | Dsm Nutricional Products Ag | Método de extração de lipídeos a partir de uma população de micro-organismos |
| AR098893A1 (es) | 2013-12-20 | 2016-06-22 | Dsm Ip Assets Bv | Proceso para obtener aceite microbiano a partir de células microbianas |
| AU2014369042B2 (en) | 2013-12-20 | 2020-04-30 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| WO2015095696A1 (en) | 2013-12-20 | 2015-06-25 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| BR112016014517B1 (pt) | 2013-12-20 | 2022-06-28 | Dsm Ip Assets B.V. | Processo para a obtenção de um óleo microbiano compreendendo um ou mais ácidos graxos poli-insaturados de uma ou mais células microbianas |
| JP2017500037A (ja) | 2013-12-20 | 2017-01-05 | ディーエスエム アイピー アセッツ ビー.ブイ. | 微生物細胞から微生物油を入手するための方法 |
| US20160002521A1 (en) * | 2014-07-03 | 2016-01-07 | Solazyme, Inc. | Lubricants and wellbore fluids |
| BR112017020483B1 (pt) | 2015-03-24 | 2021-07-06 | Terravia Holdings, Inc | Lubrificante de filme seco ou sólido e método para fornecer lubrificação a uma superfície |
| AR104042A1 (es) | 2015-03-26 | 2017-06-21 | Mara Renewables Corp | Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto |
| JP6977231B2 (ja) | 2015-07-13 | 2021-12-08 | マラ リニューアブルズ コーポレーション | C5有機炭素の微生物による代謝の増強 |
| US10851395B2 (en) | 2016-06-10 | 2020-12-01 | MARA Renewables Corporation | Method of making lipids with improved cold flow properties |
| CA3141417A1 (en) * | 2019-06-14 | 2020-12-17 | Gregory John Oliver Martin | Shear-induced phase inversion of complex emulsions for recovery of organic components from biomass |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1089093A (en) | 1963-10-03 | 1967-11-01 | British Petroleum Co | Improvements in or relating to the production of derivatives from micro-organisms |
| IL57712A (en) * | 1979-07-03 | 1984-02-29 | Yissum Res Dev Co | Cultivation of halophilic algae of the dunaliella species for the production of fuel-like product |
| JPS6012036B2 (ja) | 1982-08-06 | 1985-03-29 | 財団法人工業開発研究所 | ケイソウから油状物質を抽出する方法 |
| WO1986004354A1 (en) * | 1985-01-22 | 1986-07-31 | Japan As Represented By Director General Of Agency | Method for obtaining lipids from fungus bodies |
| US5130242A (en) * | 1988-09-07 | 1992-07-14 | Phycotech, Inc. | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
| PH11992043811B1 (en) | 1991-01-24 | 2002-08-22 | Martek Corp | Arachidonic acid and methods for the production and use thereof |
| DE69210892T2 (de) * | 1991-07-11 | 1996-10-02 | Idemitsu Petrochemical Co | Triglyceride enthaltende trockene Zellfragmente und Verfahren zu deren Herstellung |
-
1995
- 1995-07-18 GB GBGB9514649.4A patent/GB9514649D0/en active Pending
-
1996
- 1996-07-16 DE DE69613228T patent/DE69613228T2/de not_active Expired - Lifetime
- 1996-07-16 AT AT96924096T patent/ATE201907T1/de not_active IP Right Cessation
- 1996-07-16 EP EP96924096A patent/EP0843733B1/en not_active Expired - Lifetime
- 1996-07-16 DK DK96924096T patent/DK0843733T3/da active
- 1996-07-16 ES ES96924096T patent/ES2158323T3/es not_active Expired - Lifetime
- 1996-07-16 AU AU64670/96A patent/AU6467096A/en not_active Abandoned
- 1996-07-16 CA CA002226427A patent/CA2226427C/en not_active Expired - Lifetime
- 1996-07-16 US US08/983,250 patent/US6180376B1/en not_active Expired - Lifetime
- 1996-07-16 WO PCT/GB1996/001703 patent/WO1997004121A1/en not_active Ceased
- 1996-07-16 JP JP50641897A patent/JP2001507379A/ja not_active Ceased
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009501003A (ja) * | 2005-06-23 | 2009-01-15 | ディーエスエム アイピー アセッツ ビー.ブイ. | 細胞から脂質を得る方法 |
| KR101368111B1 (ko) | 2005-06-23 | 2014-02-27 | 디에스엠 아이피 어셋츠 비.브이. | 세포로부터 지질을 수득하기 위한 방법 |
| JP2010530741A (ja) * | 2007-06-14 | 2010-09-16 | ミトロプーロス,ニコラオス | バイオ燃料用の藻育成 |
| JP2016540516A (ja) * | 2013-12-20 | 2016-12-28 | マラ リニューアブルズ コーポレーション | 微生物から油を回収する方法 |
| JP2019129835A (ja) * | 2013-12-20 | 2019-08-08 | マラ リニューアブルズ コーポレーション | 微生物から油を回収する方法 |
| JP2021065234A (ja) * | 2013-12-20 | 2021-04-30 | マラ リニューアブルズ コーポレーション | 微生物から油を回収する方法 |
| JP7111434B2 (ja) | 2013-12-20 | 2022-08-02 | マラ リニューアブルズ コーポレーション | 微生物から油を回収する方法 |
| JP2022137235A (ja) * | 2013-12-20 | 2022-09-21 | マラ リニューアブルズ コーポレーション | 微生物から油を回収する方法 |
| US11746363B2 (en) | 2013-12-20 | 2023-09-05 | MARA Renewables Corporation | Methods of recovering oil from microorganisms |
| US12371721B2 (en) | 2013-12-20 | 2025-07-29 | MARA Renewables Corporation | Methods of recovering oil from microorganisms |
| JP2021529512A (ja) * | 2018-06-29 | 2021-11-04 | アミリス, インコーポレイテッド | 微生物バイオマスから不水溶性イソプレノイド化合物を回収するための方法 |
| JP7504031B2 (ja) | 2018-06-29 | 2024-06-21 | アミリス, インコーポレイテッド | 微生物バイオマスから不水溶性イソプレノイド化合物を回収するための方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| DE69613228T2 (de) | 2001-10-11 |
| US6180376B1 (en) | 2001-01-30 |
| AU6467096A (en) | 1997-02-18 |
| EP0843733A1 (en) | 1998-05-27 |
| ES2158323T3 (es) | 2001-09-01 |
| CA2226427C (en) | 2009-10-13 |
| EP0843733B1 (en) | 2001-06-06 |
| DE69613228D1 (de) | 2001-07-12 |
| GB9514649D0 (en) | 1995-09-13 |
| DK0843733T3 (da) | 2001-09-03 |
| ATE201907T1 (de) | 2001-06-15 |
| CA2226427A1 (en) | 1997-02-06 |
| WO1997004121A1 (en) | 1997-02-06 |
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