DE69534875D1 - Kalziumfreie subtilisin mutanten - Google Patents

Kalziumfreie subtilisin mutanten

Info

Publication number
DE69534875D1
DE69534875D1 DE69534875T DE69534875T DE69534875D1 DE 69534875 D1 DE69534875 D1 DE 69534875D1 DE 69534875 T DE69534875 T DE 69534875T DE 69534875 T DE69534875 T DE 69534875T DE 69534875 D1 DE69534875 D1 DE 69534875D1
Authority
DE
Germany
Prior art keywords
calcium
subtilisin mutant
free subtilisin
free
subtilisin mutants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69534875T
Other languages
English (en)
Other versions
DE69534875T2 (de
Inventor
N Bryan
A Alexander
L Strausberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Maryland Baltimore
Original Assignee
University of Maryland College Park
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Maryland College Park filed Critical University of Maryland College Park
Application granted granted Critical
Publication of DE69534875D1 publication Critical patent/DE69534875D1/de
Publication of DE69534875T2 publication Critical patent/DE69534875T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Auxiliary Methods And Devices For Loading And Unloading (AREA)
  • Cosmetics (AREA)
DE69534875T 1994-09-20 1995-04-28 Kalziumfreie subtilisin mutanten Expired - Lifetime DE69534875T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US309069 1994-09-20
US08/309,069 US5567601A (en) 1993-06-01 1994-09-20 Subtilisin mutants lacking a primary calcium binding site
PCT/US1995/005520 WO1996009396A1 (en) 1994-09-20 1995-04-28 Calcium free subtilisin mutants

Publications (2)

Publication Number Publication Date
DE69534875D1 true DE69534875D1 (de) 2006-05-11
DE69534875T2 DE69534875T2 (de) 2006-11-16

Family

ID=23196540

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69534875T Expired - Lifetime DE69534875T2 (de) 1994-09-20 1995-04-28 Kalziumfreie subtilisin mutanten

Country Status (12)

Country Link
US (1) US5567601A (de)
EP (1) EP0804592B1 (de)
JP (1) JPH10506277A (de)
KR (1) KR100364448B1 (de)
CN (1) CN1168829C (de)
AT (1) ATE320495T1 (de)
AU (1) AU693552B2 (de)
BR (1) BR9508981A (de)
CA (1) CA2200563C (de)
DE (1) DE69534875T2 (de)
NZ (1) NZ285286A (de)
WO (1) WO1996009396A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
ES2367505T3 (es) * 1997-10-23 2011-11-04 Danisco Us Inc. Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
MA24811A1 (fr) * 1997-10-23 1999-12-31 Procter & Gamble Compositions de lavage contenant des variantes de proteases multisubstituees
DE69830743T2 (de) * 1997-11-21 2006-04-27 Novozymes A/S Protease-varianten und zusammensetzungen
US6908757B1 (en) * 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
EP1082443A1 (de) * 1998-03-26 2001-03-14 The Procter & Gamble Company Serinproteasevarianten mit deletierten und substituierten aminosäuren
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
JP2002507426A (ja) 1998-03-26 2002-03-12 ザ、プロクター、エンド、ギャンブル、カンパニー アミノ酸置換を有するセリンプロテアーゼ変異体
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
AU6085800A (en) * 1999-07-22 2001-02-13 Procter & Gamble Company, The Subtilisin protease variants having amino acid substitutions in defined epitope regions
KR20020021397A (ko) * 1999-07-22 2002-03-20 데이비드 엠 모이어 규정된 에피토프 영역에 아미노산 결실 및 치환을 갖는서브틸리신 프로테아제 변이체
US6541234B1 (en) 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US20050101012A1 (en) 2001-03-12 2005-05-12 Gerold Schuler CD4+CD25+ regulatory T cells from human blood
EP1321513A1 (de) * 2001-12-21 2003-06-25 Direvo Biotech AG Subtilisin variante mit verbesserten Eigenschaften
EP1530631B1 (de) * 2002-01-16 2013-08-07 Genencor International, Inc. Mehrfachsubstituierte proteasevarianten
US8222033B2 (en) 2002-08-12 2012-07-17 Argos Therapeutics, Inc. CD4+CD25− T cells and Tr1-like regulatory T cells
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) * 2003-01-30 2007-11-13 Novozymes A/S Subtilases
WO2004083362A2 (en) * 2003-03-21 2004-09-30 Novozymes A/S Modification of subtilases using protein modelling based on the jp170 three-dimensional structure
US7727756B2 (en) 2003-03-21 2010-06-01 Novozymes A/S Subtilases
AU2004265613B2 (en) * 2003-08-06 2010-12-09 University Of Maryland, College Park Engineered proteases for affinity purification and processing of fusion proteins
US10138268B2 (en) * 2014-10-10 2018-11-27 Enzypep B.V. Peptide fragment condensation and cyclisation using a subtilisin variant with improved synthesis over hydrolysis ratio
GB201803654D0 (en) * 2018-03-07 2018-04-25 Arcticzymes As Thermolabile proteinases
PL3704143T3 (pl) * 2018-03-09 2024-04-15 Fresenius Kabi Ipsum S.R.L. Chemo-enzymatyczna synteza liraglutydu, semaglutydu i glp-1
WO2025262181A1 (en) * 2024-06-19 2025-12-26 Fresenius Kabi Ipsum S.R.L. Method to improve the producibility of a peptiligase enzyme

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
DE353250T1 (de) * 1987-04-06 1998-05-28 Novonordisk As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen.
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
JPH03505976A (ja) * 1989-05-17 1991-12-26 アムジエン・インコーポレーテツド 多重突然変異スブチリシン
CA2034486A1 (en) * 1989-06-26 1990-12-27 Eric Casteleijn Enzymatic detergent compositions
WO1991000335A1 (en) * 1989-06-26 1991-01-10 Unilever Plc Enzymatic detergent compositions
ES2110488T3 (es) * 1990-12-21 1998-02-16 Novo Nordisk As Mutantes enzimaticos con un bajo grado de variacion de la carga molecular en un intervalo de ph.
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
JP4028592B2 (ja) * 1992-07-17 2007-12-26 ジェネンコー インターナショナル インコーポレイテッド 高アルカリ性セリンプロテアーゼ
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants

Also Published As

Publication number Publication date
DE69534875T2 (de) 2006-11-16
CN1168829C (zh) 2004-09-29
CA2200563A1 (en) 1996-03-28
WO1996009396A1 (en) 1996-03-28
EP0804592B1 (de) 2006-03-15
CA2200563C (en) 2005-01-04
AU693552B2 (en) 1998-07-02
AU2433595A (en) 1996-04-09
EP0804592A1 (de) 1997-11-05
MX9702038A (es) 1997-10-31
HK1004150A1 (en) 1998-11-20
KR100364448B1 (ko) 2003-01-24
ATE320495T1 (de) 2006-04-15
NZ285286A (en) 1998-06-26
CN1166183A (zh) 1997-11-26
BR9508981A (pt) 1997-12-30
JPH10506277A (ja) 1998-06-23
US5567601A (en) 1996-10-22

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: THE UNIVERSITY OF MARYLAND, BALTIMORE, MD., US

8364 No opposition during term of opposition