ATE320495T1 - Kalziumfreie subtilisin mutanten - Google Patents

Kalziumfreie subtilisin mutanten

Info

Publication number
ATE320495T1
ATE320495T1 AT95918373T AT95918373T ATE320495T1 AT E320495 T1 ATE320495 T1 AT E320495T1 AT 95918373 T AT95918373 T AT 95918373T AT 95918373 T AT95918373 T AT 95918373T AT E320495 T1 ATE320495 T1 AT E320495T1
Authority
AT
Austria
Prior art keywords
subtilisin mutants
calcium
free subtilisin
mutants
free
Prior art date
Application number
AT95918373T
Other languages
English (en)
Inventor
Philip N Bryan
Patrick A Alexander
Susan L Strausberg
Original Assignee
Univ Maryland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland filed Critical Univ Maryland
Application granted granted Critical
Publication of ATE320495T1 publication Critical patent/ATE320495T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Cosmetics (AREA)
AT95918373T 1994-09-20 1995-04-28 Kalziumfreie subtilisin mutanten ATE320495T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/309,069 US5567601A (en) 1993-06-01 1994-09-20 Subtilisin mutants lacking a primary calcium binding site

Publications (1)

Publication Number Publication Date
ATE320495T1 true ATE320495T1 (de) 2006-04-15

Family

ID=23196540

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95918373T ATE320495T1 (de) 1994-09-20 1995-04-28 Kalziumfreie subtilisin mutanten

Country Status (13)

Country Link
US (1) US5567601A (de)
EP (1) EP0804592B1 (de)
JP (1) JPH10506277A (de)
KR (1) KR100364448B1 (de)
CN (1) CN1168829C (de)
AT (1) ATE320495T1 (de)
AU (1) AU693552B2 (de)
BR (1) BR9508981A (de)
CA (1) CA2200563C (de)
DE (1) DE69534875T2 (de)
HK (1) HK1004150A1 (de)
NZ (1) NZ285286A (de)
WO (1) WO1996009396A1 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
AR016969A1 (es) * 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
ES2367505T3 (es) * 1997-10-23 2011-11-04 Danisco Us Inc. Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
CA2310454C (en) * 1997-11-21 2012-01-24 Novo Nordisk A/S Protease variants and compositions
CA2325568A1 (en) * 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid substitutions
US6908757B1 (en) * 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
CN1303437A (zh) * 1998-03-26 2001-07-11 宝洁公司 有氨基酸缺失和替代的丝氨酸蛋白酶变体
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
AU6085800A (en) * 1999-07-22 2001-02-13 Procter & Gamble Company, The Subtilisin protease variants having amino acid substitutions in defined epitope regions
JP2003516116A (ja) 1999-07-22 2003-05-13 ザ、プロクター、エンド、ギャンブル、カンパニー 立体的に保護されたクリップ部位を有するプロテアーゼ抱合体
CN1373802A (zh) * 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
US6541234B1 (en) * 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US20050101012A1 (en) 2001-03-12 2005-05-12 Gerold Schuler CD4+CD25+ regulatory T cells from human blood
EP1321513A1 (de) * 2001-12-21 2003-06-25 Direvo Biotech AG Subtilisin variante mit verbesserten Eigenschaften
BR0306956A (pt) * 2002-01-16 2006-04-11 Genencor Int variantes de protease multiplamente substituìdas
US8222033B2 (en) 2002-08-12 2012-07-17 Argos Therapeutics, Inc. CD4+CD25− T cells and Tr1-like regulatory T cells
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
WO2004083362A2 (en) * 2003-03-21 2004-09-30 Novozymes A/S Modification of subtilases using protein modelling based on the jp170 three-dimensional structure
US7727756B2 (en) 2003-03-21 2010-06-01 Novozymes A/S Subtilases
EP1651751B1 (de) * 2003-08-06 2015-11-18 University Of Maryland Biotechnology Institute Gentechnisch hergestellte proteasen zur affinitätsreinigung und prozessierung von fusionsproteinen
EP3204401A1 (de) * 2014-10-10 2017-08-16 Enzypep B.V. Peptidfragmentkondensation und cyclisierung unter verwendung einer subtilisin-variante mit verbessertem synthese-hydrolyse-verhältnis
GB201803654D0 (en) * 2018-03-07 2018-04-25 Arcticzymes As Thermolabile proteinases
KR20200130712A (ko) * 2018-03-09 2020-11-19 엔지펩 비.브이. 리라글루티드, 세마글루티드 및 glp-1의 화학-효소적 합성

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
ES2068181T3 (es) * 1986-04-30 1995-04-16 Genencor Int Mutantes de la carbonil hidrolasa no humana, secuencias de adn y vectores que codifican las mismas y huespedes transformados con dichos vectores.
DE3856593D1 (de) * 1987-04-06 2007-10-04 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
KR100188532B1 (ko) * 1989-05-17 1999-06-01 스티븐 엠. 오드리 돌연변이 섭티리신 및 그를 함유하는 조성물
WO1991000335A1 (en) * 1989-06-26 1991-01-10 Unilever Plc Enzymatic detergent compositions
JPH04500385A (ja) * 1989-06-26 1992-01-23 ユニリーバー・ナームローゼ・ベンノートシヤープ 酵素洗剤組成物
EP0769549A3 (de) * 1990-12-21 1997-05-21 Novo Nordisk A/S Enzymmutanten mit einer geringen Variation der Molekularladung über einen pH-Bereich
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
AU682314B2 (en) * 1992-07-17 1997-10-02 Genencor International, Inc. High alkaline serine proteases
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants

Also Published As

Publication number Publication date
CN1166183A (zh) 1997-11-26
CA2200563C (en) 2005-01-04
CA2200563A1 (en) 1996-03-28
AU693552B2 (en) 1998-07-02
EP0804592B1 (de) 2006-03-15
WO1996009396A1 (en) 1996-03-28
DE69534875T2 (de) 2006-11-16
MX9702038A (es) 1997-10-31
AU2433595A (en) 1996-04-09
KR100364448B1 (ko) 2003-01-24
DE69534875D1 (de) 2006-05-11
JPH10506277A (ja) 1998-06-23
EP0804592A1 (de) 1997-11-05
CN1168829C (zh) 2004-09-29
BR9508981A (pt) 1997-12-30
NZ285286A (en) 1998-06-26
HK1004150A1 (en) 1998-11-20
US5567601A (en) 1996-10-22

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