ATE320495T1 - Kalziumfreie subtilisin mutanten - Google Patents

Kalziumfreie subtilisin mutanten

Info

Publication number
ATE320495T1
ATE320495T1 AT95918373T AT95918373T ATE320495T1 AT E320495 T1 ATE320495 T1 AT E320495T1 AT 95918373 T AT95918373 T AT 95918373T AT 95918373 T AT95918373 T AT 95918373T AT E320495 T1 ATE320495 T1 AT E320495T1
Authority
AT
Austria
Prior art keywords
subtilisin mutants
calcium
free subtilisin
mutants
free
Prior art date
Application number
AT95918373T
Other languages
English (en)
Inventor
Philip N Bryan
Patrick A Alexander
Susan L Strausberg
Original Assignee
Univ Maryland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland filed Critical Univ Maryland
Application granted granted Critical
Publication of ATE320495T1 publication Critical patent/ATE320495T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Auxiliary Methods And Devices For Loading And Unloading (AREA)
  • Cosmetics (AREA)
AT95918373T 1994-09-20 1995-04-28 Kalziumfreie subtilisin mutanten ATE320495T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/309,069 US5567601A (en) 1993-06-01 1994-09-20 Subtilisin mutants lacking a primary calcium binding site

Publications (1)

Publication Number Publication Date
ATE320495T1 true ATE320495T1 (de) 2006-04-15

Family

ID=23196540

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95918373T ATE320495T1 (de) 1994-09-20 1995-04-28 Kalziumfreie subtilisin mutanten

Country Status (12)

Country Link
US (1) US5567601A (de)
EP (1) EP0804592B1 (de)
JP (1) JPH10506277A (de)
KR (1) KR100364448B1 (de)
CN (1) CN1168829C (de)
AT (1) ATE320495T1 (de)
AU (1) AU693552B2 (de)
BR (1) BR9508981A (de)
CA (1) CA2200563C (de)
DE (1) DE69534875T2 (de)
NZ (1) NZ285286A (de)
WO (1) WO1996009396A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
MA24811A1 (fr) * 1997-10-23 1999-12-31 Procter & Gamble Compositions de lavage contenant des variantes de proteases multisubstituees
KR100762164B1 (ko) * 1997-11-21 2007-10-01 노보자임스 에이/에스 프로테아제 변이체 및 조성물
CA2324422A1 (en) * 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6908757B1 (en) * 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
AU2742499A (en) * 1998-03-26 1999-10-18 Procter & Gamble Company, The Serine protease variants having amino acid substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
CA2379729A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Protease conjugates having sterically protected clip sites
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
BR0012693A (pt) * 1999-07-22 2002-04-09 Procter & Gamble Variante, de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
BR0012660A (pt) * 1999-07-22 2002-04-09 Procter & Gamble Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
US6541234B1 (en) 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US20050101012A1 (en) 2001-03-12 2005-05-12 Gerold Schuler CD4+CD25+ regulatory T cells from human blood
EP1321513A1 (de) * 2001-12-21 2003-06-25 Direvo Biotech AG Subtilisin variante mit verbesserten Eigenschaften
US20050170488A1 (en) * 2002-01-16 2005-08-04 Poulose Ayrookaran J. Multiply-substituted protease variants
US8222033B2 (en) 2002-08-12 2012-07-17 Argos Therapeutics, Inc. CD4+CD25− T cells and Tr1-like regulatory T cells
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
EP1608744A2 (de) * 2003-03-21 2005-12-28 Novozymes A/S Subtilasen
US7727756B2 (en) 2003-03-21 2010-06-01 Novozymes A/S Subtilases
CN101454452B (zh) * 2003-08-06 2014-01-01 马里兰大学生物技术研究所 用于亲和纯化的工程蛋白酶及融合蛋白的加工
CA2962903C (en) * 2014-10-10 2023-01-10 Enzypep B.V. Peptide fragment condensation and cyclisation using a subtilisin variant with improved synthesis over hydrolysis ratio
GB201803654D0 (en) * 2018-03-07 2018-04-25 Arcticzymes As Thermolabile proteinases
ES2962666T3 (es) * 2018-03-09 2024-03-20 Fresenius Kabi Ipsum S R L Síntesis quimioenzimática de liraglutida, semaglutida y GLP-1
WO2025262181A1 (en) * 2024-06-19 2025-12-26 Fresenius Kabi Ipsum S.R.L. Method to improve the producibility of a peptiligase enzyme

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
ATE116365T1 (de) * 1986-04-30 1995-01-15 Genencor Int Mutante einer nicht menschlichen carbonyl- hydrolase, für diese kodierende dns-sequenzen und vektoren und durch diese vektoren transformierte wirte.
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
DE353250T1 (de) * 1987-04-06 1998-05-28 Novonordisk As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen.
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
KR100188532B1 (ko) * 1989-05-17 1999-06-01 스티븐 엠. 오드리 돌연변이 섭티리신 및 그를 함유하는 조성물
EP0405902A1 (de) * 1989-06-26 1991-01-02 Unilever Plc Enzymatische Waschmittelzusammensetzungen
BR9006827A (pt) * 1989-06-26 1991-08-06 Unilever Nv Composicoes detergentes enzimaticas
WO1992011357A1 (en) * 1990-12-21 1992-07-09 Novo Nordisk A/S ENZYME MUTANTS HAVING A LOW DEGREE OF VARIATION OF THE MOLECULAR CHARGE OVER A pH RANGE
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
PL178045B1 (pl) * 1992-07-17 2000-02-29 Genencor Int Mutant proteazy do stosowania w detergentach, sekwencja DNA kodująca mutanta proteazy, sposób otrzymywania mutanta proteazy oraz kompozycja detergentowa
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants

Also Published As

Publication number Publication date
DE69534875T2 (de) 2006-11-16
DE69534875D1 (de) 2006-05-11
MX9702038A (es) 1997-10-31
CN1168829C (zh) 2004-09-29
CA2200563C (en) 2005-01-04
NZ285286A (en) 1998-06-26
US5567601A (en) 1996-10-22
HK1004150A1 (en) 1998-11-20
CA2200563A1 (en) 1996-03-28
JPH10506277A (ja) 1998-06-23
AU2433595A (en) 1996-04-09
EP0804592A1 (de) 1997-11-05
EP0804592B1 (de) 2006-03-15
CN1166183A (zh) 1997-11-26
KR100364448B1 (ko) 2003-01-24
AU693552B2 (en) 1998-07-02
BR9508981A (pt) 1997-12-30
WO1996009396A1 (en) 1996-03-28

Similar Documents

Publication Publication Date Title
ATE320495T1 (de) Kalziumfreie subtilisin mutanten
ATE389666T1 (de) Hohe expressionsrate von proteinen
DE69635452D1 (de) Hohe expressionsrate des grün-fluoreszierenden proteins
DK0719340T3 (da) Klonet glutaminsyre-decarboxylase
ES2125224T3 (es) Mutantes de subtilisina.
HUP9800765A2 (hu) Kevésbé adszorbeálódó és fokozottan hidrolizáló szubtilizin Carlsberg variánsok
ATE310098T1 (de) Dna kodierend für glutaminsäure-gesteuerte chloridkanäle
DE69731608D1 (de) Verbessertes barstar-gen
DE69535696D1 (de) Tripeptidyl-aminopeptidase
SE8801701D0 (sv) Production technology based on enzymic method
ES8708013A1 (es) Procedimiento para preparar plasmidos de expresion prhw11 y prhw12
AU8258591A (en) Human mk gene and protein sequence
DE59712235D1 (de) Immunologisch aktive proteine von borrelia burgdorferi, dafür kodierende nukleinsäuren sowie deren verwendung in testkits und als impfstoffe
AU3162097A (en) Retinoid metabolizing protein
DE69737476D1 (de) Neuer galaninrezeptor
PT977859E (pt) O factor neurotrofico nnt-1
ATE252641T1 (de) Erhöhung der proteinproduktion in gram-positiven mikroorganismen
DE69829670D1 (de) Erhöhung der proteinproduktion in gram-positiven mikroorganismen
GB2329896A (en) Enzyme and DNA sequence encoding same
SE9604439D0 (sv) New receptor
AU5779690A (en) Mutation of the rhinovirus system
DE3783425D1 (de) Mikroorganismen, die fremdproteinenthaltende pili tragen, isolierte pili, verfahren zur ausscheidung von proteinen in verwendung dieser pili und deren nutzen.
ATE398681T1 (de) Nukleotidsequenzen die für das mene-gen kodieren

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties