DE69533443D1 - Die herstellung von biologisch aktiven, rekombinanten und neurotrophem protein - Google Patents

Die herstellung von biologisch aktiven, rekombinanten und neurotrophem protein

Info

Publication number
DE69533443D1
DE69533443D1 DE69533443T DE69533443T DE69533443D1 DE 69533443 D1 DE69533443 D1 DE 69533443D1 DE 69533443 T DE69533443 T DE 69533443T DE 69533443 T DE69533443 T DE 69533443T DE 69533443 D1 DE69533443 D1 DE 69533443D1
Authority
DE
Germany
Prior art keywords
neurotrophic factor
biologically active
production
recombinant
neurotrophemic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69533443T
Other languages
English (en)
Other versions
DE69533443T2 (de
Inventor
Jack Lile
Tadahiko Kohno
Duane Bonam
Mary S Rosendahl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of DE69533443D1 publication Critical patent/DE69533443D1/de
Application granted granted Critical
Publication of DE69533443T2 publication Critical patent/DE69533443T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]
    • C07K14/503—Fibroblast growth factor [FGF] basic FGF [bFGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/48—Nerve growth factor [NGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69533443T 1994-05-09 1995-05-02 Die herstellung von biologisch aktiven, rekombinanten und neurotrophem protein Expired - Lifetime DE69533443T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US24012294A 1994-05-09 1994-05-09
US240122 1994-05-09
US266080 1994-06-27
US08/266,080 US5606031A (en) 1990-04-06 1994-06-27 Production and purification of biologically active recombinant neurotrophic protein in bacteria
PCT/US1995/005423 WO1995030686A1 (en) 1994-05-09 1995-05-02 Production of biologically active recombinant neurotrophic protein

Publications (2)

Publication Number Publication Date
DE69533443D1 true DE69533443D1 (de) 2004-10-07
DE69533443T2 DE69533443T2 (de) 2005-01-20

Family

ID=26933155

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69533443T Expired - Lifetime DE69533443T2 (de) 1994-05-09 1995-05-02 Die herstellung von biologisch aktiven, rekombinanten und neurotrophem protein

Country Status (11)

Country Link
US (1) US5606031A (de)
EP (1) EP0772627B1 (de)
JP (1) JP3673278B2 (de)
AT (1) ATE275152T1 (de)
AU (1) AU697891B2 (de)
CA (1) CA2189659C (de)
DE (1) DE69533443T2 (de)
DK (1) DK0772627T3 (de)
ES (1) ES2230549T3 (de)
PT (1) PT772627E (de)
WO (1) WO1995030686A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE206056T1 (de) * 1992-06-08 2001-10-15 Takeda Chemical Industries Ltd Therapeutisches agens für neutropenie
US6299895B1 (en) 1997-03-24 2001-10-09 Neurotech S.A. Device and method for treating ophthalmic diseases
PL191400B1 (pl) * 1996-11-15 2006-05-31 Genentech Inc Sposób izolowania neurotrofiny z mieszaniny białek i kompozycja neurotrofiny
TR200003024T2 (tr) 1998-04-17 2000-12-21 Innogenetics N.V. İndirgeme ajanlarını kullanarak geliştirilen immünodiagnostik tahliller.
ES2213861T3 (es) * 1998-10-09 2004-09-01 Scil Proteins Gmbh Procedimiento de obtencion de ngf-beta activa.
US6451987B1 (en) * 1999-03-15 2002-09-17 Novo Nordisk A/S Ion exchange chromatography of proteins and peptides
US6444788B1 (en) * 1999-03-15 2002-09-03 Novo Nordisk A/S Ion exchange chromatography of GLP-1, analogs and derivatives thereof
WO2001011085A2 (en) 1999-08-11 2001-02-15 Whitehead Institute For Biomedical Research Bdnf polymorphism and association with bipolar disorder
AU7061700A (en) 1999-08-17 2001-03-13 University Of Texas System, The Use of colostrinin, constituent peptides thereof, and analogs thereof as oxidative stress regulators
US6903068B1 (en) 1999-08-17 2005-06-07 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof for inducing cytokines
US6852685B1 (en) 1999-08-17 2005-02-08 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof to promote neuronal cell differentiation
US7119064B2 (en) 1999-08-17 2006-10-10 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof as modulators of intracellular signaling molecules
AU2001273348A1 (en) * 2000-07-10 2002-01-21 Diosynth Rtp, Inc. Purification of human troponin i
WO2002013851A1 (en) * 2000-08-17 2002-02-21 The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof to promote neural cell differentiation
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
EP1233075A3 (de) * 2001-02-15 2003-01-08 Whitehead Institute For Biomedical Research BDNF Polymorphismus und Vereinigung mit doppelpoliger Geistesgestörtheit
US20030232365A1 (en) * 2001-02-15 2003-12-18 Whitehead Institute For Biomedical Research BDNF polymorphisms and association with bipolar disorder
US20030012818A1 (en) * 2001-04-25 2003-01-16 Eidgenossische Technische Hochschule Zurich And Universitat Zurich Drug delivery matrices to enhance wound healing
ZA200700168B (en) 2001-10-10 2010-02-24 Novo Nordisk As Remodeling and glycoconjugation of peptides
EP2292271A3 (de) 2001-10-10 2011-09-14 BioGeneriX AG Neumodulierung und Glykokonjugation von Antikörpern
SG155777A1 (en) 2003-04-09 2009-10-29 Neose Technologies Inc Glycopegylation methods and proteins/peptides produced by the methods
CA2532340A1 (en) * 2003-08-21 2005-03-03 Novo Nordisk A/S Separation of polypeptides comprising a racemized amino acid
JP2012512812A (ja) * 2007-12-28 2012-06-07 クロス・バイオサージェリー・アクチェンゲゼルシャフト フィブリンフォームに組込まれたｐｄｇｆ融合タンパク質
JP2014506119A (ja) 2010-12-02 2014-03-13 ニューロテック ユーエスエー， インコーポレイテッド 抗血管新生抗体足場および可溶性受容体を分泌する細胞株およびその使用
ES2833457T3 (es) 2015-05-27 2021-06-15 Neurotech Usa Inc Uso de terapia con células encapsuladas para el tratamiento del glaucoma

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4223967A (en) * 1978-05-10 1980-09-23 Antonio Royer Telescopic structural element for making modular closets, partition walls, door supports and the like
US4421685A (en) * 1980-03-27 1983-12-20 Eli Lilly And Company Process for producing an insulin
US4620948A (en) * 1982-12-22 1986-11-04 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US5169762A (en) * 1983-03-03 1992-12-08 Genentech, Inc. Human nerve growth factor by recombinant technology
DK161152C (da) * 1983-03-03 1991-11-11 Genentech Inc Polypeptid med egenskaber som human beta-nervevaekstfaktor og fremgangsmaade til fremstilling deraf, dna-isolat omfattende en sekvens som koder for polypeptidet, replicerbar udtrykkelsesvektor for dna-sekvensen, rekombinant vaertscelle transformeret med vektoren, farmaceutisk praeparat indeholdende polypeptidet og fremg. der omfatter anvendelsen af polypeptidet til fremst. af et farmaceutisk praeparat
US4451396A (en) * 1983-08-01 1984-05-29 Eli Lilly And Company Process for inhibiting undesired thiol reactions during cyanogen bromide cleavage of peptides
JPH01257491A (ja) * 1988-04-08 1989-10-13 Tosoh Corp 不溶性融合異種蛋白質の処理方法
US4923967A (en) * 1988-09-26 1990-05-08 Eli Lilly And Company Purification and refolding of recombinant proteins
IL95511A (en) * 1989-08-30 2000-10-31 Max Planck Gesellschaft Neurotrophin-3 a novel neurotrophic factor related to nerve growth and brain derived neurotrophic factor
US5180820A (en) * 1989-08-30 1993-01-19 Barde Yves Alain Brain-derived neurotrophic factor
US5235043A (en) * 1990-04-06 1993-08-10 Synergen, Inc. Production of biologically active, recombinant members of the ngf/bdnf family of neurotrophic proteins
US5169764A (en) * 1990-08-08 1992-12-08 Regeneron Pharmaceuticals, Inc. Multitrophic and multifunctional chimeric neurotrophic factors, and nucleic acids and plasmids encoding the chimeras
DE4139000A1 (de) * 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf

Also Published As

Publication number Publication date
AU697891B2 (en) 1998-10-22
ATE275152T1 (de) 2004-09-15
AU2432095A (en) 1995-11-29
JP3673278B2 (ja) 2005-07-20
WO1995030686A1 (en) 1995-11-16
PT772627E (pt) 2005-01-31
ES2230549T3 (es) 2005-05-01
CA2189659A1 (en) 1995-11-16
JPH10502804A (ja) 1998-03-17
EP0772627B1 (de) 2004-09-01
DK0772627T3 (da) 2004-11-29
EP0772627A1 (de) 1997-05-14
EP0772627A4 (de) 1997-06-11
US5606031A (en) 1997-02-25
DE69533443T2 (de) 2005-01-20
CA2189659C (en) 2006-12-12

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Legal Events

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