DE69527355D1 - Herstellung von nukleinsaeurekopien - Google Patents

Herstellung von nukleinsaeurekopien

Info

Publication number
DE69527355D1
DE69527355D1 DE69527355T DE69527355T DE69527355D1 DE 69527355 D1 DE69527355 D1 DE 69527355D1 DE 69527355 T DE69527355 T DE 69527355T DE 69527355 T DE69527355 T DE 69527355T DE 69527355 D1 DE69527355 D1 DE 69527355D1
Authority
DE
Germany
Prior art keywords
strands
target
copy target
oligonucleotides
copy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69527355T
Other languages
English (en)
Other versions
DE69527355T2 (de
Inventor
John Minter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tepnel Medical Ltd
Original Assignee
Tepnel Medical Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9410804A external-priority patent/GB9410804D0/en
Priority claimed from GBGB9502079.8A external-priority patent/GB9502079D0/en
Application filed by Tepnel Medical Ltd filed Critical Tepnel Medical Ltd
Publication of DE69527355D1 publication Critical patent/DE69527355D1/de
Application granted granted Critical
Publication of DE69527355T2 publication Critical patent/DE69527355T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69527355T 1994-05-28 1995-05-30 Herstellung von nukleinsaeurekopien Expired - Lifetime DE69527355T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9410804A GB9410804D0 (en) 1994-05-28 1994-05-28 Producing copies of nucleic acids
GBGB9502079.8A GB9502079D0 (en) 1995-02-02 1995-02-02 Producing copies of nucleic acids
PCT/GB1995/001237 WO1995033073A1 (en) 1994-05-28 1995-05-30 Producing copies of nucleic acids

Publications (2)

Publication Number Publication Date
DE69527355D1 true DE69527355D1 (de) 2002-08-14
DE69527355T2 DE69527355T2 (de) 2003-03-06

Family

ID=26304963

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69527355T Expired - Lifetime DE69527355T2 (de) 1994-05-28 1995-05-30 Herstellung von nukleinsaeurekopien

Country Status (11)

Country Link
US (1) US5922574A (de)
EP (1) EP0763135B1 (de)
JP (1) JPH10500862A (de)
CN (1) CN1152946A (de)
AT (1) ATE220424T1 (de)
AU (1) AU707391B2 (de)
CA (1) CA2191426A1 (de)
DE (1) DE69527355T2 (de)
FI (1) FI964735A (de)
NZ (1) NZ287118A (de)
WO (1) WO1995033073A1 (de)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406848B1 (en) 1997-05-23 2002-06-18 Lynx Therapeutics, Inc. Planar arrays of microparticle-bound polynucleotides
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
GB9503808D0 (en) * 1995-02-24 1995-04-12 Univ Nottingham Detection assay
GB2308188A (en) * 1995-12-14 1997-06-18 Tepnel Medical Ltd Assaying immobilised nucleic acid by primer extension
ATE545710T1 (de) 1997-04-01 2012-03-15 Illumina Cambridge Ltd Verfahren zur vervielfältigung von nukleinsäuren
GB9707980D0 (en) 1997-04-21 1997-06-11 Brax Genomics Ltd Characterising DNA
AU736776B2 (en) 1997-07-22 2001-08-02 Qiagen Genomics, Inc. Apparatus and methods for arraying solution onto a solid support
WO1999005321A1 (en) * 1997-07-22 1999-02-04 Rapigene, Inc. Amplification and other enzymatic reactions performed on nucleic acid arrays
US5916776A (en) * 1997-08-27 1999-06-29 Sarnoff Corporation Amplification method for a polynucleotide
CA2335951C (en) 1998-06-24 2013-07-30 Mark S. Chee Decoding of array sensors with microspheres
US20040157256A1 (en) * 1998-09-17 2004-08-12 Taisho Pharmaceutical Co., Ltd. Immobilized cDNA libraries
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
WO2000058516A2 (en) * 1999-03-26 2000-10-05 Whitehead Institute For Biomedical Research Universal arrays
EP1471926B8 (de) 1999-06-01 2011-09-21 Baylor College Of Medicine Zusammensetzungen und verfahren zur therapeutischen anwendung einer mit dem gen atonal assoziierten sequenz
US7771975B2 (en) * 2001-04-02 2010-08-10 Point-2-Point Genomics Limited Polynucleotide analysis using combinatorial PCR
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
WO2003064621A2 (en) 2002-02-01 2003-08-07 Ambion, Inc. HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
EP2221377B2 (de) 2002-02-01 2017-05-17 Life Technologies Corporation Doppelsträngige Oligonukleotide
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
US6916474B2 (en) 2002-07-15 2005-07-12 Board Of Regents, The University Of Texas System Antibodies with increased affinities for anthrax antigens
EP2044948B1 (de) 2002-08-12 2013-10-30 Jennerex Biotherapeutics ULC Vacciniaviren zur Anwendung in der Behandlung von Krebs
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
CN1878866B (zh) 2003-07-25 2012-08-01 安比恩股份有限公司 用于从固定的样品中制备rna的方法和组合物
DE602005021525D1 (de) 2004-09-14 2010-07-08 Univ Colorado R behandlung mit bucindolol
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
CA2621982C (en) * 2005-09-07 2017-11-28 Jennerex Biotherapeutics Ulc Systemic treatment of metastatic and/or systemically-disseminated cancers using gm-csf-expressing poxviruses
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
EP2368570A3 (de) 2006-01-18 2012-05-02 University Of Chicago Zusammensetzungen und Verfahren in Verbindung mit Stayphylokokken-Bakterienproteinen
EP1987159B2 (de) 2006-02-08 2020-08-12 Illumina Cambridge Limited Verfahren zur sequenzierung einer polynukleotidmatrize
CN102026645B (zh) 2006-09-15 2016-01-27 渥太华医院研究机构 溶瘤弹状病毒
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
CA2671264C (en) 2006-11-30 2015-11-24 Research Development Foundation Improved immunoglobulin libraries
EP2121983A2 (de) 2007-02-02 2009-11-25 Illumina Cambridge Limited Verfahren zur kennzeichnung von proben und sequenzierung mehrerer nukleotidmatrizen
EP2155789B1 (de) 2007-05-01 2013-07-24 Research Development Foundation Immunglobulin-fc-bibliotheken
EP2191011B1 (de) * 2007-08-29 2017-03-29 Illumina Cambridge Limited Verfahren zur sequenzierung einer polynukleotidmatrize
US8999642B2 (en) 2008-03-10 2015-04-07 Illumina, Inc. Methods for selecting and amplifying polynucleotides
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
JP2012504660A (ja) 2008-10-06 2012-02-23 ユニバーシティ オブ シカゴ 細菌ｅａｐ、ｅｍｐおよび／またはａｄｓａタンパク質に関連する組成物および方法
KR20170102039A (ko) 2009-04-03 2017-09-06 유니버시티 오브 시카고 단백질 Ａ(ＳｐＡ) 변이체와 관련된 조성물 및 방법
US9587270B2 (en) 2009-06-29 2017-03-07 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
US9409983B2 (en) 2009-07-23 2016-08-09 The Board Of Trustess Of The University Of Illinois Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases
US9512481B2 (en) 2009-09-11 2016-12-06 The Regents Of The University Of Colorado, A Body Corporate Polymorphisms in the PDE3A gene
ES2848650T3 (es) 2009-09-14 2021-08-11 Sillajen Biotherapeutics Inc Terapia combinada contra el cáncer con virus vaccinia oncolítico
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
CN102858959B (zh) 2009-12-10 2016-02-24 渥太华医院研究院 溶瘤弹状病毒
US20130131161A1 (en) 2009-12-23 2013-05-23 Michael R. Bristow Methods and compositions for cardiovascular diseases and conditions
JP2013523818A (ja) 2010-04-05 2013-06-17 ザ・ユニバーシティー・オブ・シカゴ 免疫反応のエンハンサーとしてのプロテインＡ（ＳｐＡ）抗体に関連する組成物および方法
KR20130093084A (ko) 2010-07-02 2013-08-21 더 유니버시티 오브 시카고 단백질 Ａ(ＳｐＡ) 변이체와 관련된 조성물 및 방법
JP5793194B2 (ja) 2010-09-09 2015-10-14 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ 感染防御ブドウ球菌抗原が関与する方法および組成物
JP6121910B2 (ja) 2011-01-04 2017-04-26 シラジェン バイオセラピューティクス インコーポレイテッド 腫瘍溶解性ワクシニアウイルスの投与による腫瘍抗原に対する抗体の生成および腫瘍特異的補体依存性細胞傷害の生成
US8952132B2 (en) 2011-02-07 2015-02-10 Research Development Foundation Engineered immunoglobulin FC polypeptides
MX2013009863A (es) 2011-02-28 2013-12-06 Univ Iowa Res Found Cambios de la hormona anti-mulleriana en el embarazo y prediccion de sexo y de resultados adversos en el embarazo.
CA2829300A1 (en) 2011-03-08 2012-09-13 King Abdullah University Of Science And Technology Molecular biomarker set for early detection of ovarian cancer
EP3406628A1 (de) 2011-04-08 2018-11-28 Evaxion Biotech ApS In impfstoffen gegen staphylococcus aureus nützliche proteine und nukleinsäuren
US8945588B2 (en) 2011-05-06 2015-02-03 The University Of Chicago Methods and compositions involving protective staphylococcal antigens, such as EBH polypeptides
ES2627529T3 (es) 2011-06-08 2017-07-28 Children's Hospital Of Eastern Ontario Research Institute Inc. Composiciones para tratamiento de glioblastoma
US10040853B2 (en) 2011-09-09 2018-08-07 Fred Hutchinson Cancer Research Center Methods and compositions involving NKG2D inhibitors and cancer
US9273102B2 (en) 2011-10-12 2016-03-01 Niels Iversen Møller Peptides derived from Campylobacter jejuni and their use in vaccination
EP2794904B1 (de) 2011-12-22 2017-09-06 Ibis Biosciences, Inc. Amplifizierung einer sequenz aus einer ribonucleinsäure
EP2809783A2 (de) 2012-02-02 2014-12-10 Invenra, Inc. Hochdurchsatz-screen für biologisch aktive polypeptide
EP3805395A1 (de) 2012-04-26 2021-04-14 University Of Chicago Staphylokokken-coagulase-antigene und verfahren zu deren verwendung
WO2014116721A1 (en) 2013-01-22 2014-07-31 The Arizona Board Of Regents For And On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
US10363293B2 (en) 2013-02-21 2019-07-30 Turnstone Limited Partnership Vaccine composition
SG11201602540XA (en) 2013-10-03 2016-04-28 Oklahoma Med Res Found Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
EP4227685A3 (de) 2013-12-03 2024-02-28 Evaxion Biotech A/S Proteine und nukleinsäuren für impfstoffe gegen staphylococcus aureus
WO2016075305A2 (en) 2014-11-13 2016-05-19 Evaxion Biotech Aps Peptides derived from acinetobacter baumannii and their use in vaccination
EP3224362A4 (de) 2014-11-26 2018-06-06 The Regents of The University of California Therapeutische zusammensetzungen mit transkriptionsfaktoren sowie verfahren zur herstellung und verwendung davon
EP4279080A3 (de) 2015-01-12 2024-02-21 Evaxion Biotech A/S Behandlung und prophylaxe von k.-pneumoniae-infektionen
WO2016130516A1 (en) 2015-02-09 2016-08-18 Research Development Foundation Engineered immunoglobulin fc polypeptides displaying improved complement activation
EP3070178B1 (de) 2015-03-20 2020-02-12 Albert-Ludwigs-Universität Freiburg Verfahren zur diagnose von brustkrebs
EP3317295B1 (de) 2015-07-04 2022-05-18 Evaxion Biotech A/S Proteine und nukleinsäuren, die in impfstoffen gegen pseudomonas aeruginosa nützlich sind
WO2017040380A2 (en) 2015-08-28 2017-03-09 Research Development Foundation Engineered antibody fc variants
WO2017144523A1 (en) 2016-02-22 2017-08-31 Evaxion Biotech Aps Proteins and nucleic acids useful in vaccines targeting staphylococcus aureus
WO2018127545A1 (en) 2017-01-05 2018-07-12 Evaxion Biotech Aps Vaccines targeting pseudomonas aeruginosa
AU2018213315A1 (en) 2017-01-26 2019-07-25 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
AU2019360758A1 (en) 2018-10-18 2021-05-20 Oklahoma Medical Research Foundation Biomarkers for a systemic lupus erythematosus (SLE) disease activity immune index that characterizes disease activity
WO2020083904A1 (en) 2018-10-22 2020-04-30 Evaxion Biotech Aps Vaccines targeting m. catharrhalis
EP3931206A1 (de) 2019-02-27 2022-01-05 Evaxion Biotech ApS Impfstoffe gegen h. influenzae
EP3772543B1 (de) 2019-08-09 2023-09-27 Albert-Ludwigs-Universität Freiburg Verfahren zur diagnose von brustkrebs
EP4087593A1 (de) 2020-01-06 2022-11-16 Evaxion Biotech A/S Impfstoffe, die gegen neisseria gonorrhoeae gerichtet sind
EP4366762A1 (de) 2021-07-05 2024-05-15 Evaxion Biotech A/S Impfstoffe gegen neisseria gonorrhoeae
WO2023239940A1 (en) 2022-06-10 2023-12-14 Research Development Foundation Engineered fcriib selective igg1 fc variants and uses thereof

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CH663728A5 (fr) * 1985-06-10 1988-01-15 Battelle Memorial Institute Procede de purification de substances bioactives par adsorption biospecifique.
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
CA1317535C (en) * 1987-06-30 1993-05-11 Nanibhushan Dattagupta Assay of sequences using amplified genes
AU2735988A (en) * 1987-12-21 1989-07-13 Amoco Corporation Target and background capture methods with amplification for affinity assays
SE8801070D0 (sv) * 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US5153166A (en) * 1988-08-18 1992-10-06 Trustees Of At Biochem Chromatographic stationary supports
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
GB8827160D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
IL92474A (en) * 1988-11-29 1994-04-12 Orion Yhtymae Oy Reaction method and combination for determining nucleotide sequences
CA2004326A1 (en) * 1988-12-23 1990-06-23 Nanibushan Dattagupta Assay of sequences using amplified genes
WO1990009455A1 (en) * 1989-02-13 1990-08-23 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
CA2011818A1 (en) * 1989-03-17 1990-09-17 Fred T. Oakes Methods for purification, amplification and detection of a nucleic acid
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
EP0519016A1 (de) * 1990-03-07 1992-12-23 F.Hoffmann-La Roche & Co. Aktiengesellschaft Verfahren zur diagnose der lyme-krankheit
DK0455905T3 (da) * 1990-05-11 1998-12-07 Microprobe Corp Dipsticks til nukleinsyrehybridiseringsassays og fremgangsmåde til kovalent immobilisering af oligonukleotider
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
CA2055755A1 (en) * 1990-11-22 1992-05-23 Toshihiko Kishimoto Method of immobilizing single-stranded dna on carrier at terminal
US5437976A (en) * 1991-08-08 1995-08-01 Arizona Board Of Regents, The University Of Arizona Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
WO1993004199A2 (en) * 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
BR9206705A (pt) * 1991-11-01 1995-11-21 Adelaide Children S Hospital Processo de amplificação de fase sólida
RU2186852C2 (ru) * 1991-12-24 2002-08-10 Тепнел Медикал Лимитед Способ выполнения манипуляции с последовательностью нуклеиновой кислоты, устройство для проведения манипуляций на последовательности нуклеиновой кислоты, проточный сосуд, твердый носитель для иммобилизации последовательности нуклеиновой кислоты, твердый носитель, способ получения носителя для иммобилизации последовательности нуклеиновой кислоты
JPH08500722A (ja) * 1992-01-29 1996-01-30 日立化成工業株式会社 ポリヌクレオチド固定化担体
EP0672187A4 (de) * 1992-10-08 1999-11-17 Univ California Pcr-testverfahren zur bestimmung der anwesenheit und konzentration einer zielsubstanz.
GB9322723D0 (en) * 1993-11-04 1993-12-22 Tepnel Medical Ltd Determining nucleic acids
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support

Also Published As

Publication number Publication date
AU2572895A (en) 1995-12-21
JPH10500862A (ja) 1998-01-27
EP0763135B1 (de) 2002-07-10
EP0763135A1 (de) 1997-03-19
FI964735A0 (fi) 1996-11-27
NZ287118A (en) 1999-01-28
FI964735A (fi) 1997-01-27
US5922574A (en) 1999-07-13
CN1152946A (zh) 1997-06-25
AU707391B2 (en) 1999-07-08
WO1995033073A1 (en) 1995-12-07
DE69527355T2 (de) 2003-03-06
ATE220424T1 (de) 2002-07-15
CA2191426A1 (en) 1995-12-07

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