DE69334157D1 - Expressionsvektor pUC NT und ihre Verwendung - Google Patents

Expressionsvektor pUC NT und ihre Verwendung

Info

Publication number
DE69334157D1
DE69334157D1 DE69334157T DE69334157T DE69334157D1 DE 69334157 D1 DE69334157 D1 DE 69334157D1 DE 69334157 T DE69334157 T DE 69334157T DE 69334157 T DE69334157 T DE 69334157T DE 69334157 D1 DE69334157 D1 DE 69334157D1
Authority
DE
Germany
Prior art keywords
expression vector
vector puc
puc
expression
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69334157T
Other languages
English (en)
Other versions
DE69334157T2 (de
Inventor
Yasuhiro Ikenaka
Hirokazu Nanba
Masayuki Takano
Kazuyoshi Yajima
Yukio Yamada
Satomi Takahashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kanegafuchi Chemical Industry Co Ltd
Original Assignee
Kanegafuchi Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kanegafuchi Chemical Industry Co Ltd filed Critical Kanegafuchi Chemical Industry Co Ltd
Publication of DE69334157D1 publication Critical patent/DE69334157D1/de
Application granted granted Critical
Publication of DE69334157T2 publication Critical patent/DE69334157T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69334157T 1992-08-10 1993-08-05 Expressionsvektor pUC NT und ihre Verwendung Expired - Lifetime DE69334157T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP21269292 1992-08-10
JP21269292 1992-08-10
JP34007892 1992-12-21
JP34007892 1992-12-21

Publications (2)

Publication Number Publication Date
DE69334157D1 true DE69334157D1 (de) 2007-09-06
DE69334157T2 DE69334157T2 (de) 2008-04-17

Family

ID=26519366

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69334157T Expired - Lifetime DE69334157T2 (de) 1992-08-10 1993-08-05 Expressionsvektor pUC NT und ihre Verwendung
DE69333668T Expired - Fee Related DE69333668T2 (de) 1992-08-10 1993-08-05 Für eine decarbamylase mit verbesserter thermostabilität kodierende dna und ihre verwendung

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69333668T Expired - Fee Related DE69333668T2 (de) 1992-08-10 1993-08-05 Für eine decarbamylase mit verbesserter thermostabilität kodierende dna und ihre verwendung

Country Status (9)

Country Link
US (1) US6083752A (de)
EP (2) EP1043398B1 (de)
JP (1) JP3438890B2 (de)
KR (1) KR100266920B1 (de)
CN (2) CN1040025C (de)
DE (2) DE69334157T2 (de)
ES (1) ES2231772T3 (de)
SG (1) SG54275A1 (de)
WO (1) WO1994003613A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994008030A1 (en) * 1992-10-05 1994-04-14 Kanegafuchi Chemical Industry Co., Ltd. PROCESS FOR PRODUCING D-α-AMINO ACID
JPH07114789B2 (ja) * 1993-08-26 1995-12-13 工業技術院長 上肢動作補助機構
IT1269321B (it) * 1994-04-15 1997-03-26 Eniricerche Spa Mutanti stabili della d-n- -carbamilasi
US5962279A (en) * 1994-06-24 1999-10-05 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Process for producing D-amino acids with composite immobilized enzyme preparation
IT1277125B1 (it) * 1995-12-21 1997-11-04 Eniricerche Spa Mutanti termostabili della d-n-alfa-carbamilasi
JP2001069981A (ja) * 1999-08-31 2001-03-21 Kanegafuchi Chem Ind Co Ltd デカルバミラーゼの立体構造およびその利用法
JP4561009B2 (ja) 2001-07-10 2010-10-13 味の素株式会社 Ｄ−ヒダントインハイドロラーゼをコードするｄｎａ、ｎ−カルバミル−ｄ−アミノ酸ハイドロラーゼをコードするｄｎａ、該遺伝子を含む組み換えｄｎａ、該組み換えｄｎａにより形質転換された細胞、該形質転換細胞を用いるタンパク質の製造方法、および、ｄ−アミノ酸の製造方法
EP1712627B1 (de) * 2004-02-04 2010-09-22 Kaneka Corporation Polypeptid mit amidaseaktivität und dessen gen
US20070292923A1 (en) 2004-08-06 2007-12-20 Akira Iwasaki Process For Production Of Enantiomer-Enriched Compounds
EP1811021A4 (de) 2004-10-27 2008-05-28 Kaneka Corp Neue carbonylreduktase, gen dafür und verwendung davon
US20100003732A1 (en) 2005-05-31 2010-01-07 Kaneka Corporation Process for production of optically active 2-substituted propanal derivative
JP5291934B2 (ja) 2005-08-02 2013-09-18 株式会社カネカ Ｄ−アミノ酸オキシダーゼ、およびｌ−アミノ酸、２−オキソ酸、又は環状イミンの製造方法。
WO2007040272A1 (ja) * 2005-10-06 2007-04-12 Kaneka Corporation Ｄ－（４－アミノメチル）フェニルアラニン誘導体の製造法
JPWO2007094178A1 (ja) 2006-02-14 2009-07-02 株式会社カネカ 新規な（ｓ，ｓ）−ブタンジオール脱水素酵素、その遺伝子、及びその利用法
JP5005672B2 (ja) * 2006-02-28 2012-08-22 株式会社カネカ 新規カルボニル還元酵素、その遺伝子、およびそれらを利用した光学活性アルコールの製造方法
US8168412B2 (en) 2006-05-29 2012-05-01 Kaneka Corporation Method for producing optically-active amine compound, recombinant vector, and transformant containing the vector
US9273332B2 (en) 2006-10-12 2016-03-01 Kaneka Corporation Method for production of L-amino acid
KR100858913B1 (ko) * 2007-03-09 2008-09-17 한국과학기술원 부위특이적 변이에 의해 제조된 고수율의 ｌ－쓰레오닌생산균주 및 이를 이용한 ｌ－쓰레오닌의 제조방법
JP5603076B2 (ja) 2007-09-26 2014-10-08 株式会社カネカ 新規グルコース脱水素酵素
WO2010067578A1 (ja) 2008-12-09 2010-06-17 株式会社カネカ 新規なアミノ酸脱水素酵素、およびｌ－アミノ酸、２－オキソ酸、又はｄ－アミノ酸の製造方法
JP5761641B2 (ja) 2009-04-23 2015-08-12 株式会社カネカ （ｒ）−３−キヌクリジノールの製造方法
JP5889785B2 (ja) 2010-04-22 2016-03-22 株式会社カネカ 改変型カルボニル還元酵素、その遺伝子、およびそれらを利用した光学活性アルコールの製造方法
US9481871B2 (en) 2010-09-28 2016-11-01 Kaneka Corporation Nucleic acid encoding a polpeptide having aminotransferase activity, vectors and host cells comprising the nucleic acid
CN103209949B (zh) 2010-11-09 2016-08-10 株式会社钟化 卤化茚酮类及使用其的光学活性茚满酮类或光学活性茚满醇类的制造方法
WO2012124639A1 (ja) 2011-03-11 2012-09-20 株式会社カネカ 改変型アミノ基転移酵素、その遺伝子、およびそれらを利用した光学活性アミノ化合物の製造方法
JP6442399B2 (ja) 2013-03-28 2018-12-19 株式会社カネカ 改変型カルボニル還元酵素およびその遺伝子
WO2018084165A1 (ja) 2016-11-01 2018-05-11 株式会社カネカ 改変型酵素およびその利用
WO2018203482A1 (ja) 2017-05-01 2018-11-08 株式会社カネカ Ａｔｐを利用した物質の製造方法
CN114807103B (zh) * 2022-06-10 2023-07-04 铜陵利夫生物科技有限公司 一种氨基甲酰基水解酶突变体及基因和应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3819463A1 (de) * 1988-06-08 1989-12-14 Behringwerke Ag Expressionsvektoren zur herstellung unfusionierter proteine in mikroorganismen
JPH01320991A (ja) * 1988-06-20 1989-12-27 Kanegafuchi Chem Ind Co Ltd Ｄ−ホモフエニルアラニン類の製造方法
DE3841454A1 (de) * 1988-12-09 1990-06-13 Degussa Verfahren zur ortsspezifischen mutagenese von dna und entwicklung von plasmidvektoren
US5824522A (en) * 1990-12-07 1998-10-20 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Recombinant decarbamylases for producing D-α-amino acids
WO1992010579A1 (en) * 1990-12-07 1992-06-25 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha PROCESS FOR PRODUCING D-α-AMINO ACID
JPH09210579A (ja) * 1996-01-31 1997-08-12 Calsonic Corp オイルクーラのスタッドボルト取付部構造

Also Published As

Publication number Publication date
WO1994003613A1 (en) 1994-02-17
CN1083528A (zh) 1994-03-09
DE69333668D1 (de) 2004-11-25
DE69333668T2 (de) 2006-02-23
SG54275A1 (en) 1998-11-16
EP1043398A1 (de) 2000-10-11
JP3438890B2 (ja) 2003-08-18
EP0610517B1 (de) 2004-10-20
ES2231772T3 (es) 2005-05-16
KR100266920B1 (ko) 2003-12-31
US6083752A (en) 2000-07-04
EP1043398B1 (de) 2007-07-25
EP0610517A1 (de) 1994-08-17
CN1040025C (zh) 1998-09-30
CN1168415A (zh) 1997-12-24
CN1108383C (zh) 2003-05-14
EP0610517A4 (en) 1998-06-10
DE69334157T2 (de) 2008-04-17

Similar Documents

Publication Publication Date Title
DE69334157D1 (de) Expressionsvektor pUC NT und ihre Verwendung
DE69413636D1 (de) Reibungsmodifizierende Zusammensetzungen und ihre Verwendung
DE69333697D1 (de) Härtbare Zusammensetzungen und Verwendung derselben
DE69322062T2 (de) Emulsionspolymere und ihre verwendung in haarfestigungszubereitungen
NO180408C (no) Brannlukkeanordning
FI944624A (fi) 4-aryyliaminobentsopyraani- ja niiden kaltaisia yhdisteitä
ZA931632B (en) Novel arylindazoles and their use as herbicides
DE69418203D1 (de) Zementiermittel und ihre verwendung
DE59401465D1 (de) Pulverlack und seine Verwendung
DE69406010T2 (de) Wässrige alkylpolyglycosidkonzentrate und ihre verwendung
DE69414028T2 (de) Lasersystem und Verwendung des Lasersystems
DE69333528D1 (de) D-n-carbamoylaminosaureamidohydrolase und hydantoinase
ATE168107T1 (de) 4-benzoylisoxazole-derivate und ihre verwendung als herbizide
DE69333264D1 (de) 3-Phenyl-5-pyridyl-Triazole und ihre Verwendung als Insektizide
DE59307396D1 (de) Dichtring und verwendung desselben
DE69332815T2 (de) Polyglyzidyl-Äthern und ihre Verwendung
DE69421235D1 (de) Amidrazone und ihre Verwendung als Pestizide
DE59407240D1 (de) Substituierte azadioxacycloalkene und ihre verwendung als fungizide
DE59208370D1 (de) Stromrichterschaltungsanordnung und ihre Verwendung
ZA93425B (en) Novel arylindoles and their use as herbicides
FI1031U1 (fi) En troakar
ITMI921571A1 (it) Azaciclo e azabiciclo alchiliden idrossilamine
DE69328467D1 (de) Polyamidzusammensetzung und Verwendung
DE69412083D1 (de) Phenylpyrrolderivate und ihre verwendung als antipsychotika
DE59303212D1 (de) Gar- und kochvorrichtung

Legal Events

Date Code Title Description
8364 No opposition during term of opposition