DE69233206T2 - Methode zur herstellung rekombinanter proteine und dafür verwendete wirtszellen - Google Patents
Methode zur herstellung rekombinanter proteine und dafür verwendete wirtszellen Download PDFInfo
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- DE69233206T2 DE69233206T2 DE69233206T DE69233206T DE69233206T2 DE 69233206 T2 DE69233206 T2 DE 69233206T2 DE 69233206 T DE69233206 T DE 69233206T DE 69233206 T DE69233206 T DE 69233206T DE 69233206 T2 DE69233206 T2 DE 69233206T2
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- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 239000006481 glucose medium Substances 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000009830 intercalation Methods 0.000 description 1
- 230000002687 intercalation Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000003016 pheromone Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000892 thaumatin Substances 0.000 description 1
- 235000010436 thaumatin Nutrition 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9108217A FR2678636A1 (fr) | 1991-07-02 | 1991-07-02 | Procede de production de proteines recombinantes et cellules hote utilisees. |
| FR9108217 | 1991-07-02 | ||
| PCT/FR1992/000610 WO1993001274A1 (fr) | 1991-07-02 | 1992-06-30 | Procede de production de proteines recombinantes et cellules hotes utilisees |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69233206D1 DE69233206D1 (de) | 2003-10-23 |
| DE69233206T2 true DE69233206T2 (de) | 2004-07-01 |
Family
ID=9414582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69233206T Expired - Lifetime DE69233206T2 (de) | 1991-07-02 | 1992-06-30 | Methode zur herstellung rekombinanter proteine und dafür verwendete wirtszellen |
Country Status (14)
| Country | Link |
|---|---|
| EP (2) | EP0592574B1 (https=) |
| JP (1) | JPH06509226A (https=) |
| AT (1) | ATE250126T1 (https=) |
| AU (1) | AU2278392A (https=) |
| CA (1) | CA2110992A1 (https=) |
| DE (1) | DE69233206T2 (https=) |
| DK (1) | DK0592574T3 (https=) |
| ES (1) | ES2203611T3 (https=) |
| FR (1) | FR2678636A1 (https=) |
| IE (1) | IE922148A1 (https=) |
| NO (1) | NO934572L (https=) |
| NZ (1) | NZ243403A (https=) |
| PT (1) | PT592574E (https=) |
| WO (1) | WO1993001274A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063080A (zh) * | 2015-09-07 | 2015-11-18 | 复旦大学 | 不含有信号肽的在马克斯克鲁维酵母营养缺陷型菌株中进行外源基因表达的重组载体 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2608540B2 (ja) * | 1982-05-19 | 1997-05-07 | ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ | クルイベロミセス種のためのクローニング系 |
| IT1203758B (it) * | 1986-03-27 | 1989-02-23 | Univ Roma | Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori |
| NO302485B1 (no) * | 1987-07-28 | 1998-03-09 | Gist Brocades Bv | Fremgangsmåte for fremstilling av et polypeptid av interesse i en Klyveromyces vertscelle, transformert Klyveromyces vertscelle og anvendelse derav, plasmid og DNA konstruksjon |
| FR2649991B2 (fr) * | 1988-08-05 | 1994-03-04 | Rhone Poulenc Sante | Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces |
-
1991
- 1991-07-02 FR FR9108217A patent/FR2678636A1/fr active Granted
-
1992
- 1992-06-30 AT AT92915114T patent/ATE250126T1/de active
- 1992-06-30 DE DE69233206T patent/DE69233206T2/de not_active Expired - Lifetime
- 1992-06-30 CA CA002110992A patent/CA2110992A1/fr not_active Abandoned
- 1992-06-30 DK DK92915114T patent/DK0592574T3/da active
- 1992-06-30 JP JP5502002A patent/JPH06509226A/ja active Pending
- 1992-06-30 ES ES92915114T patent/ES2203611T3/es not_active Expired - Lifetime
- 1992-06-30 AU AU22783/92A patent/AU2278392A/en not_active Abandoned
- 1992-06-30 WO PCT/FR1992/000610 patent/WO1993001274A1/fr not_active Ceased
- 1992-06-30 EP EP92915114A patent/EP0592574B1/fr not_active Expired - Lifetime
- 1992-06-30 PT PT92915114T patent/PT592574E/pt unknown
- 1992-06-30 EP EP92401848A patent/EP0521767A1/fr active Pending
- 1992-07-01 NZ NZ243403A patent/NZ243403A/en unknown
- 1992-07-01 IE IE214892A patent/IE922148A1/en not_active Application Discontinuation
-
1993
- 1993-12-13 NO NO934572A patent/NO934572L/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JPH06509226A (ja) | 1994-10-20 |
| WO1993001274A1 (fr) | 1993-01-21 |
| NO934572D0 (no) | 1993-12-13 |
| EP0592574B1 (fr) | 2003-09-17 |
| EP0521767A1 (fr) | 1993-01-07 |
| CA2110992A1 (fr) | 1993-01-21 |
| EP0592574A1 (fr) | 1994-04-20 |
| PT592574E (pt) | 2004-02-27 |
| NZ243403A (en) | 1994-12-22 |
| DE69233206D1 (de) | 2003-10-23 |
| FR2678636A1 (fr) | 1993-01-08 |
| FR2678636B1 (https=) | 1994-08-19 |
| ATE250126T1 (de) | 2003-10-15 |
| DK0592574T3 (da) | 2004-01-19 |
| ES2203611T3 (es) | 2004-04-16 |
| IE922148A1 (en) | 1993-01-13 |
| NO934572L (no) | 1993-12-13 |
| AU2278392A (en) | 1993-02-11 |
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| 8328 | Change in the person/name/address of the agent |
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