DE69123973T2 - Analytisches verfahren - Google Patents
Analytisches verfahrenInfo
- Publication number
- DE69123973T2 DE69123973T2 DE69123973T DE69123973T DE69123973T2 DE 69123973 T2 DE69123973 T2 DE 69123973T2 DE 69123973 T DE69123973 T DE 69123973T DE 69123973 T DE69123973 T DE 69123973T DE 69123973 T2 DE69123973 T2 DE 69123973T2
- Authority
- DE
- Germany
- Prior art keywords
- cell
- harmonic
- potential
- biological
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004458 analytical method Methods 0.000 title description 2
- 239000000463 material Substances 0.000 claims abstract description 20
- 239000012620 biological material Substances 0.000 claims abstract description 13
- 230000004044 response Effects 0.000 claims abstract description 9
- 239000000758 substrate Substances 0.000 claims abstract description 9
- 239000003112 inhibitor Substances 0.000 claims abstract description 7
- 230000019522 cellular metabolic process Effects 0.000 claims abstract description 6
- 239000000523 sample Substances 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 24
- 239000000725 suspension Substances 0.000 claims description 15
- 239000006285 cell suspension Substances 0.000 claims description 8
- 230000001419 dependent effect Effects 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 claims description 2
- 239000006185 dispersion Substances 0.000 claims 1
- 238000001228 spectrum Methods 0.000 abstract description 27
- 238000001453 impedance spectrum Methods 0.000 abstract description 24
- 230000009021 linear effect Effects 0.000 abstract description 15
- 210000004027 cell Anatomy 0.000 description 58
- 102000004190 Enzymes Human genes 0.000 description 15
- 108090000790 Enzymes Proteins 0.000 description 15
- 230000000694 effects Effects 0.000 description 12
- 239000012528 membrane Substances 0.000 description 12
- 230000005284 excitation Effects 0.000 description 7
- 102000015176 Proton-Translocating ATPases Human genes 0.000 description 6
- 108010039518 Proton-Translocating ATPases Proteins 0.000 description 6
- 210000000170 cell membrane Anatomy 0.000 description 5
- 230000005684 electric field Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 108010052285 Membrane Proteins Proteins 0.000 description 4
- 241000191938 Micrococcus luteus Species 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 4
- 230000003068 static effect Effects 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000000284 resting effect Effects 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- LSGOVYNHVSXFFJ-UHFFFAOYSA-N vanadate(3-) Chemical compound [O-][V]([O-])([O-])=O LSGOVYNHVSXFFJ-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003989 dielectric material Substances 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- CMZUMMUJMWNLFH-UHFFFAOYSA-N sodium metavanadate Chemical compound [Na+].[O-][V](=O)=O CMZUMMUJMWNLFH-UHFFFAOYSA-N 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229940121819 ATPase inhibitor Drugs 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 239000000362 adenosine triphosphatase inhibitor Substances 0.000 description 1
- 238000000191 alpha-particle scattering Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000005574 cross-species transmission Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000004720 dielectrophoresis Methods 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 230000005686 electrostatic field Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000001566 impedance spectroscopy Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 150000003893 lactate salts Chemical class 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 150000003682 vanadium compounds Chemical class 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48735—Investigating suspensions of cells, e.g. measuring microbe concentration
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Mechanical Treatment Of Semiconductor (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Iron Core Of Rotating Electric Machines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB909018886A GB9018886D0 (en) | 1990-08-30 | 1990-08-30 | Non linear dielectric |
| GB9111893A GB2247530B (en) | 1990-08-30 | 1991-06-03 | Analytical method |
| PCT/GB1991/001477 WO1992004630A1 (en) | 1990-08-30 | 1991-08-30 | Analytical method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69123973D1 DE69123973D1 (de) | 1997-02-13 |
| DE69123973T2 true DE69123973T2 (de) | 1997-07-03 |
Family
ID=26297566
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69123973T Expired - Fee Related DE69123973T2 (de) | 1990-08-30 | 1991-08-30 | Analytisches verfahren |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0546044B1 (ja) |
| JP (1) | JP2964166B2 (ja) |
| AT (1) | ATE147165T1 (ja) |
| AU (1) | AU649070B2 (ja) |
| CA (1) | CA2090699A1 (ja) |
| DE (1) | DE69123973T2 (ja) |
| WO (1) | WO1992004630A1 (ja) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9215733D0 (en) * | 1992-07-24 | 1992-09-09 | British Tech Group | Method of and apparatus for determining a property of a sample |
| GB9315881D0 (en) * | 1993-07-31 | 1993-09-15 | Univ Wales | Electrode |
| DE4328689A1 (de) * | 1993-08-26 | 1995-03-02 | Beiersdorf Ag | Verfahren zur Detektion und Zählung von Mikroorganismen |
| AUPM851694A0 (en) * | 1994-09-30 | 1994-10-27 | Barsamian, Sergei T | New methods for diagnosis, detection of cell abnormalities and morphology of living systems |
| AU747114B2 (en) * | 1994-09-30 | 2002-05-09 | Electronic Diagnostics Pty Ltd | Method and apparatus for diagnosis, detection of cell abnormalities and morphology of living systems |
| JP2002501802A (ja) * | 1998-02-04 | 2002-01-22 | ダーマル セラピー （バルバドス） インク． | 体液中のグルコースを非侵襲的に測定するための方法及び装置 |
| GB0000721D0 (en) * | 2000-01-14 | 2000-03-08 | Univ Wales Aberystwyth The | Protective coatings for metallic electrodes |
| KR100601494B1 (ko) * | 2000-11-23 | 2006-07-14 | 에스케이 주식회사 | 유전센서를 이용한 오일의 염분 함량 측정 방법 및 시스템 |
| JP4599566B2 (ja) * | 2004-08-31 | 2010-12-15 | 国立大学法人 新潟大学 | 不均一電場を使用した非極性複合分子の運動の電気的検出法 |
| FR2942041B1 (fr) * | 2009-02-06 | 2011-02-25 | Commissariat Energie Atomique | Dispositif embarque d'analyse d'un fluide corporel. |
| PL219558B1 (pl) | 2011-04-21 | 2015-05-29 | Marcin Just | Urządzenie i sposób do diagnostyki próchnicy wtórnej |
| DE102016203576A1 (de) * | 2016-03-04 | 2017-09-07 | Hamilton Bonaduz Ag | Verfahren zur Kalibration von impedanzspektroskopischen Biomassesensoren und Verwendung einer Suspension zur Durchführung eines solchen Verfahrens |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU520939B2 (en) * | 1977-06-17 | 1982-03-11 | Unisearch Limited | Measuring impedance of membrane layers |
| DE3325843A1 (de) * | 1983-07-18 | 1985-02-07 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | Verfahren und vorrichtung zur unterscheidung von in einem medium befindlichen teilchen oder partikeln |
| GB8805488D0 (en) * | 1988-03-08 | 1988-04-07 | Health Lab Service Board | Measuring electrical impedance of low conductivity samples |
-
1991
- 1991-08-30 DE DE69123973T patent/DE69123973T2/de not_active Expired - Fee Related
- 1991-08-30 AU AU84957/91A patent/AU649070B2/en not_active Ceased
- 1991-08-30 AT AT91915873T patent/ATE147165T1/de not_active IP Right Cessation
- 1991-08-30 CA CA002090699A patent/CA2090699A1/en not_active Abandoned
- 1991-08-30 JP JP3514605A patent/JP2964166B2/ja not_active Expired - Fee Related
- 1991-08-30 EP EP91915873A patent/EP0546044B1/en not_active Expired - Lifetime
- 1991-08-30 WO PCT/GB1991/001477 patent/WO1992004630A1/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| CA2090699A1 (en) | 1992-03-01 |
| DE69123973D1 (de) | 1997-02-13 |
| JP2964166B2 (ja) | 1999-10-18 |
| EP0546044B1 (en) | 1997-01-02 |
| ATE147165T1 (de) | 1997-01-15 |
| WO1992004630A1 (en) | 1992-03-19 |
| EP0546044A1 (en) | 1993-06-16 |
| AU8495791A (en) | 1992-03-30 |
| JPH06504613A (ja) | 1994-05-26 |
| AU649070B2 (en) | 1994-05-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |