DE69034257D1 - Hitzestabile gereinigte Endoglucanasen aus thermophilem Bakterium Acidothermus Cellulolyticus - Google Patents

Hitzestabile gereinigte Endoglucanasen aus thermophilem Bakterium Acidothermus Cellulolyticus

Info

Publication number
DE69034257D1
DE69034257D1 DE69034257T DE69034257T DE69034257D1 DE 69034257 D1 DE69034257 D1 DE 69034257D1 DE 69034257 T DE69034257 T DE 69034257T DE 69034257 T DE69034257 T DE 69034257T DE 69034257 D1 DE69034257 D1 DE 69034257D1
Authority
DE
Germany
Prior art keywords
heat
thermophilic bacterium
acidothermus cellulolyticus
stable purified
endoglucanases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69034257T
Other languages
English (en)
Inventor
Ali Mohagheghi
Melvin P Tucker
Michael E Himmel
Karel Grohmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Midwest Research Institute
Original Assignee
Midwest Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Midwest Research Institute filed Critical Midwest Research Institute
Application granted granted Critical
Publication of DE69034257D1 publication Critical patent/DE69034257D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
DE69034257T 1989-09-26 1990-08-27 Hitzestabile gereinigte Endoglucanasen aus thermophilem Bakterium Acidothermus Cellulolyticus Expired - Lifetime DE69034257D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/412,434 US5110735A (en) 1989-09-26 1989-09-26 Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus

Publications (1)

Publication Number Publication Date
DE69034257D1 true DE69034257D1 (de) 2008-09-18

Family

ID=23632963

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69032772T Expired - Lifetime DE69032772T2 (de) 1989-09-26 1990-08-27 Hitzestabile gereinigte cellulase aus dem thermophilen bakterium acidothermus cellulolyticus
DE69034257T Expired - Lifetime DE69034257D1 (de) 1989-09-26 1990-08-27 Hitzestabile gereinigte Endoglucanasen aus thermophilem Bakterium Acidothermus Cellulolyticus

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69032772T Expired - Lifetime DE69032772T2 (de) 1989-09-26 1990-08-27 Hitzestabile gereinigte cellulase aus dem thermophilen bakterium acidothermus cellulolyticus

Country Status (6)

Country Link
US (1) US5110735A (de)
EP (2) EP0494207B1 (de)
JP (1) JPH072113B2 (de)
AU (1) AU6420290A (de)
DE (2) DE69032772T2 (de)
WO (1) WO1991005039A1 (de)

Families Citing this family (169)

* Cited by examiner, † Cited by third party
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US5432075A (en) * 1989-09-26 1995-07-11 Midwest Research Institute Low molecular weight thermostable β-D-glucosidase from acidothermus cellulolyticus
US5275944A (en) * 1989-09-26 1994-01-04 Midwest Research Institute Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068
US5712142A (en) * 1989-09-26 1998-01-27 Midwest Research Institute Method for increasing thermostability in cellulase ennzymes
US5536655A (en) * 1989-09-26 1996-07-16 Midwest Research Institute Gene coding for the E1 endoglucanase
US5366884A (en) * 1989-09-26 1994-11-22 Midwest Research Institute Thermostable purified endoglucanase II from Acidothermus cellulolyticus ATCC
LT3208B (en) * 1992-04-10 1995-03-27 Ssv Dev Oy Enzyme products for use in the improvement of feed value and conservation of fibrous crops
CA2139001A1 (en) * 1993-05-10 1994-11-24 Jean Paul Vincken Combined action of endoglucanases and cellobiohydrolases
US5514584A (en) * 1993-06-11 1996-05-07 Midwest Research Institute Cloning of cellulase genes from acidothermus cellulolyticus
CN1308537C (zh) * 1997-12-19 2007-04-04 诺沃奇梅兹北美公司 含纤维素织物的连续生物抛光
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US5110735A (en) 1992-05-05
AU6420290A (en) 1991-04-28
DE69032772T2 (de) 1999-06-24
EP0885955A3 (de) 1999-04-07
WO1991005039A1 (en) 1991-04-18
EP0494207A1 (de) 1992-07-15
JPH072113B2 (ja) 1995-01-18
EP0885955A2 (de) 1998-12-23
JPH05500752A (ja) 1993-02-18
DE69032772D1 (de) 1998-12-24
EP0885955B1 (de) 2008-08-06
EP0494207A4 (en) 1992-09-16

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