DE68919360D1 - Verfahren zur reinigung hochreiner heparinase in grosser menge. - Google Patents

Verfahren zur reinigung hochreiner heparinase in grosser menge.

Info

Publication number
DE68919360D1
DE68919360D1 DE68919360T DE68919360T DE68919360D1 DE 68919360 D1 DE68919360 D1 DE 68919360D1 DE 68919360 T DE68919360 T DE 68919360T DE 68919360 T DE68919360 T DE 68919360T DE 68919360 D1 DE68919360 D1 DE 68919360D1
Authority
DE
Germany
Prior art keywords
heparinase
approximately
organisms
stabilized medium
daltons
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68919360T
Other languages
English (en)
Other versions
DE68919360T2 (de
Inventor
Joseph Zimmerman
Charles Cooney
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of DE68919360D1 publication Critical patent/DE68919360D1/de
Application granted granted Critical
Publication of DE68919360T2 publication Critical patent/DE68919360T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE68919360T 1988-06-06 1989-06-02 Verfahren zur reinigung hochreiner heparinase in grosser menge. Expired - Lifetime DE68919360T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20323588A 1988-06-06 1988-06-06
PCT/US1989/002434 WO1989012692A1 (en) 1988-06-06 1989-06-02 Large scale method for purification of high purity heparinase

Publications (2)

Publication Number Publication Date
DE68919360D1 true DE68919360D1 (de) 1994-12-15
DE68919360T2 DE68919360T2 (de) 1995-05-18

Family

ID=22753090

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68919360T Expired - Lifetime DE68919360T2 (de) 1988-06-06 1989-06-02 Verfahren zur reinigung hochreiner heparinase in grosser menge.

Country Status (6)

Country Link
EP (1) EP0420894B1 (de)
JP (2) JP2603349B2 (de)
AT (1) ATE113991T1 (de)
CA (1) CA1341079C (de)
DE (1) DE68919360T2 (de)
WO (1) WO1989012692A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714376A (en) * 1991-10-23 1998-02-03 Massachusetts Institute Of Technology Heparinase gene from flavobacterium heparinum
US5389539A (en) 1992-11-30 1995-02-14 Massachusetts Institute Of Technology Purification of heparinase I, II, and III from Flavobacterium heparinum
DE4429660B4 (de) * 1994-08-20 2004-02-12 Dade Behring Marburg Gmbh Zusatzmittel für diagnostische Tests zur Bestimmung der Gerinnungsfähigkeit des Blutes, Verfahren zur Reduzierung der Beeinflussung von diagnostischen Tests durch Heparin und Verwendung von Metallsalzen zu diesen Zwecken
EP1109919A2 (de) 1998-08-27 2001-06-27 Massachusetts Institute Of Technology Von heparinase i und ii abgeleitete heparinasen
JP2007501622A (ja) * 2003-08-13 2007-02-01 サンド・アクチエンゲゼルシヤフト 組換えポリペプチドを精製するための方法
US8211668B2 (en) * 2007-01-12 2012-07-03 Dow Agrosciences, Llc. Apparatus and methods for osmotically shocking cells
EP3561048A4 (de) 2016-12-22 2020-08-12 Shenzhen Hepalink Pharmaceutical Group Co., Ltd. Heparinase-produzierender pseudomonas-stutzeri-stamm und heparinase daraus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54107584A (en) * 1978-02-10 1979-08-23 Seikagaku Kogyo Co Ltd Preparation of pure heparinase
US4341869A (en) * 1980-08-25 1982-07-27 Massachusetts Institute Of Technology Process for producing heparinase

Also Published As

Publication number Publication date
JP2869039B2 (ja) 1999-03-10
JP2603349B2 (ja) 1997-04-23
EP0420894A1 (de) 1991-04-10
EP0420894B1 (de) 1994-11-09
JPH09149788A (ja) 1997-06-10
ATE113991T1 (de) 1994-11-15
WO1989012692A1 (en) 1989-12-28
EP0420894A4 (en) 1992-03-18
DE68919360T2 (de) 1995-05-18
JPH03505815A (ja) 1991-12-19
CA1341079C (en) 2000-08-08

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