ATE113991T1 - Verfahren zur reinigung hochreiner heparinase in grosser menge. - Google Patents
Verfahren zur reinigung hochreiner heparinase in grosser menge.Info
- Publication number
- ATE113991T1 ATE113991T1 AT89907019T AT89907019T ATE113991T1 AT E113991 T1 ATE113991 T1 AT E113991T1 AT 89907019 T AT89907019 T AT 89907019T AT 89907019 T AT89907019 T AT 89907019T AT E113991 T1 ATE113991 T1 AT E113991T1
- Authority
- AT
- Austria
- Prior art keywords
- heparinase
- approximately
- purification
- organisms
- stabilized medium
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20323588A | 1988-06-06 | 1988-06-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE113991T1 true ATE113991T1 (de) | 1994-11-15 |
Family
ID=22753090
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT89907019T ATE113991T1 (de) | 1988-06-06 | 1989-06-02 | Verfahren zur reinigung hochreiner heparinase in grosser menge. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0420894B1 (de) |
JP (2) | JP2603349B2 (de) |
AT (1) | ATE113991T1 (de) |
CA (1) | CA1341079C (de) |
DE (1) | DE68919360T2 (de) |
WO (1) | WO1989012692A1 (de) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5714376A (en) * | 1991-10-23 | 1998-02-03 | Massachusetts Institute Of Technology | Heparinase gene from flavobacterium heparinum |
US5389539A (en) * | 1992-11-30 | 1995-02-14 | Massachusetts Institute Of Technology | Purification of heparinase I, II, and III from Flavobacterium heparinum |
DE4429660B4 (de) * | 1994-08-20 | 2004-02-12 | Dade Behring Marburg Gmbh | Zusatzmittel für diagnostische Tests zur Bestimmung der Gerinnungsfähigkeit des Blutes, Verfahren zur Reduzierung der Beeinflussung von diagnostischen Tests durch Heparin und Verwendung von Metallsalzen zu diesen Zwecken |
SI1656455T1 (sl) * | 2003-08-13 | 2012-12-31 | Sandoz Ag | Postopek za čiščenje rekombinantnih polipeptidov |
CN101646780A (zh) * | 2007-01-12 | 2010-02-10 | 陶氏环球技术公司 | 渗透休克细胞的设备与方法 |
WO2018113775A1 (zh) | 2016-12-22 | 2018-06-28 | 深圳市海普瑞药业集团股份有限公司 | 产肝素酶的施氏假单胞菌菌株及从其衍生的肝素酶 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54107584A (en) * | 1978-02-10 | 1979-08-23 | Seikagaku Kogyo Co Ltd | Preparation of pure heparinase |
US4341869A (en) * | 1980-08-25 | 1982-07-27 | Massachusetts Institute Of Technology | Process for producing heparinase |
-
1989
- 1989-06-02 DE DE68919360T patent/DE68919360T2/de not_active Expired - Lifetime
- 1989-06-02 AT AT89907019T patent/ATE113991T1/de not_active IP Right Cessation
- 1989-06-02 JP JP1506606A patent/JP2603349B2/ja not_active Expired - Lifetime
- 1989-06-02 WO PCT/US1989/002434 patent/WO1989012692A1/en active IP Right Grant
- 1989-06-02 EP EP89907019A patent/EP0420894B1/de not_active Expired - Lifetime
- 1989-06-06 CA CA000601943A patent/CA1341079C/en not_active Expired - Fee Related
-
1996
- 1996-06-25 JP JP8164991A patent/JP2869039B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO1989012692A1 (en) | 1989-12-28 |
DE68919360T2 (de) | 1995-05-18 |
DE68919360D1 (de) | 1994-12-15 |
EP0420894A1 (de) | 1991-04-10 |
JP2603349B2 (ja) | 1997-04-23 |
JPH03505815A (ja) | 1991-12-19 |
CA1341079C (en) | 2000-08-08 |
JP2869039B2 (ja) | 1999-03-10 |
EP0420894B1 (de) | 1994-11-09 |
EP0420894A4 (en) | 1992-03-18 |
JPH09149788A (ja) | 1997-06-10 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EELA | Cancelled due to lapse of time |