JPS54107584A - Preparation of pure heparinase - Google Patents

Preparation of pure heparinase

Info

Publication number
JPS54107584A
JPS54107584A JP1454878A JP1454878A JPS54107584A JP S54107584 A JPS54107584 A JP S54107584A JP 1454878 A JP1454878 A JP 1454878A JP 1454878 A JP1454878 A JP 1454878A JP S54107584 A JPS54107584 A JP S54107584A
Authority
JP
Japan
Prior art keywords
heparinase
purified
cation exchange
adsorbent
column chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1454878A
Other languages
Japanese (ja)
Inventor
Zensaku Yoshizawa
Noboru Otoya
Masateru Kikuchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Priority to JP1454878A priority Critical patent/JPS54107584A/en
Publication of JPS54107584A publication Critical patent/JPS54107584A/en
Pending legal-status Critical Current

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Abstract

PURPOSE: To produce a highly pure heparinase free from other acidic mucopolysaccharase, in large quantities, by treating a roughly purified heparinase solution with column chromatography using a cationic exchanging material as adsorbent.
CONSTITUTION: Bacterial cells obtained by the culturing of heparinase-producing bacteria, e.g. Flavobacterium heparinum, in a medium containing heparin, are disintegrated by ultrasonic treatment, etc. The disintegrated cells are extracted, and preliminary purified by protamin sulfate treatment, hydroxyapatite fractionation, etc. The purified heparinase is obtained by the column chromatography of the preliminary purified heparin using a cation exchange resin, as an adsorbent. The cation exchanger is, e.g. a cation exchange cellulose, cation exchange crosslinked dextran, etc., and pref. phosphocellulose, phosphonomethylcellulose, etc.
COPYRIGHT: (C)1979,JPO&Japio
JP1454878A 1978-02-10 1978-02-10 Preparation of pure heparinase Pending JPS54107584A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1454878A JPS54107584A (en) 1978-02-10 1978-02-10 Preparation of pure heparinase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1454878A JPS54107584A (en) 1978-02-10 1978-02-10 Preparation of pure heparinase

Publications (1)

Publication Number Publication Date
JPS54107584A true JPS54107584A (en) 1979-08-23

Family

ID=11864195

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1454878A Pending JPS54107584A (en) 1978-02-10 1978-02-10 Preparation of pure heparinase

Country Status (1)

Country Link
JP (1) JPS54107584A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63503194A (en) * 1986-03-07 1988-11-24 ザ　チルドレンズ　メデイカル　センタ−　コ−ポレイシヨン heparin test
EP0420894A1 (en) * 1988-06-06 1991-04-10 Massachusetts Inst Technology Large scale method for purification of high purity heparinase.
US5169772A (en) * 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
EP0670892A4 (en) * 1992-11-30 1997-05-28 Massachusetts Inst Technology PURIFICATION, COMPOSITION AND SPECIFICITY OF HEPARINASE I, II, AND III FROM -i(FLAVOBACTERIUM HEPARINUM).
US5714376A (en) * 1991-10-23 1998-02-03 Massachusetts Institute Of Technology Heparinase gene from flavobacterium heparinum
CN101891842A (en) * 2010-07-16 2010-11-24 杭州龙扬生物科技有限公司 Process for producing heparin sodium

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63503194A (en) * 1986-03-07 1988-11-24 ザ　チルドレンズ　メデイカル　センタ−　コ−ポレイシヨン heparin test
EP0420894A1 (en) * 1988-06-06 1991-04-10 Massachusetts Inst Technology Large scale method for purification of high purity heparinase.
JPH03505815A (en) * 1988-06-06 1991-12-19 マサチューセッツ インスティテュート オブ テクノロジー Large-scale purification method for high-purity heparinase
EP0420894A4 (en) * 1988-06-06 1992-03-18 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase
US5169772A (en) * 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
EP0420894B1 (en) * 1988-06-06 1994-11-09 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase
US5714376A (en) * 1991-10-23 1998-02-03 Massachusetts Institute Of Technology Heparinase gene from flavobacterium heparinum
EP0670892A4 (en) * 1992-11-30 1997-05-28 Massachusetts Inst Technology PURIFICATION, COMPOSITION AND SPECIFICITY OF HEPARINASE I, II, AND III FROM -i(FLAVOBACTERIUM HEPARINUM).
USRE41328E1 (en) 1992-11-30 2010-05-11 Massachusetts Institute Of Technology Purification of Heparinase I, II and III from Flavobacterium heparinum
USRE41461E1 (en) 1992-11-30 2010-07-27 Massachusetts Institute Of Technology Purification, composition and specificity of Heparinase I, II, and III from Flavobacterium heparinum
CN101891842A (en) * 2010-07-16 2010-11-24 杭州龙扬生物科技有限公司 Process for producing heparin sodium

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