DE68919360T2 - Verfahren zur reinigung hochreiner heparinase in grosser menge. - Google Patents

Verfahren zur reinigung hochreiner heparinase in grosser menge.

Info

Publication number
DE68919360T2
DE68919360T2 DE68919360T DE68919360T DE68919360T2 DE 68919360 T2 DE68919360 T2 DE 68919360T2 DE 68919360 T DE68919360 T DE 68919360T DE 68919360 T DE68919360 T DE 68919360T DE 68919360 T2 DE68919360 T2 DE 68919360T2
Authority
DE
Germany
Prior art keywords
heparinase
approximately
organisms
stabilized medium
daltons
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68919360T
Other languages
English (en)
Other versions
DE68919360D1 (de
Inventor
Joseph Zimmerman
Charles Cooney
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of DE68919360D1 publication Critical patent/DE68919360D1/de
Application granted granted Critical
Publication of DE68919360T2 publication Critical patent/DE68919360T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE68919360T 1988-06-06 1989-06-02 Verfahren zur reinigung hochreiner heparinase in grosser menge. Expired - Lifetime DE68919360T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20323588A 1988-06-06 1988-06-06
PCT/US1989/002434 WO1989012692A1 (en) 1988-06-06 1989-06-02 Large scale method for purification of high purity heparinase

Publications (2)

Publication Number Publication Date
DE68919360D1 DE68919360D1 (de) 1994-12-15
DE68919360T2 true DE68919360T2 (de) 1995-05-18

Family

ID=22753090

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68919360T Expired - Lifetime DE68919360T2 (de) 1988-06-06 1989-06-02 Verfahren zur reinigung hochreiner heparinase in grosser menge.

Country Status (6)

Country Link
EP (1) EP0420894B1 (de)
JP (2) JP2603349B2 (de)
AT (1) ATE113991T1 (de)
CA (1) CA1341079C (de)
DE (1) DE68919360T2 (de)
WO (1) WO1989012692A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714376A (en) * 1991-10-23 1998-02-03 Massachusetts Institute Of Technology Heparinase gene from flavobacterium heparinum
US5389539A (en) 1992-11-30 1995-02-14 Massachusetts Institute Of Technology Purification of heparinase I, II, and III from Flavobacterium heparinum
DE4429660B4 (de) * 1994-08-20 2004-02-12 Dade Behring Marburg Gmbh Zusatzmittel für diagnostische Tests zur Bestimmung der Gerinnungsfähigkeit des Blutes, Verfahren zur Reduzierung der Beeinflussung von diagnostischen Tests durch Heparin und Verwendung von Metallsalzen zu diesen Zwecken
WO2005017174A2 (en) * 2003-08-13 2005-02-24 Sandoz Ag Process for the purification of recombinant polypeptides
US8211668B2 (en) * 2007-01-12 2012-07-03 Dow Agrosciences, Llc. Apparatus and methods for osmotically shocking cells
US10900028B2 (en) 2016-12-22 2021-01-26 Shenzhen Hepalink Pharmaceutical Group Co., Ltd. Heparinase-producing Pseudomonas stutzeri strain and heparinase derived therefrom

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54107584A (en) * 1978-02-10 1979-08-23 Seikagaku Kogyo Co Ltd Preparation of pure heparinase
US4341869A (en) * 1980-08-25 1982-07-27 Massachusetts Institute Of Technology Process for producing heparinase

Also Published As

Publication number Publication date
JPH09149788A (ja) 1997-06-10
DE68919360D1 (de) 1994-12-15
CA1341079C (en) 2000-08-08
ATE113991T1 (de) 1994-11-15
EP0420894B1 (de) 1994-11-09
WO1989012692A1 (en) 1989-12-28
JP2603349B2 (ja) 1997-04-23
JPH03505815A (ja) 1991-12-19
JP2869039B2 (ja) 1999-03-10
EP0420894A4 (en) 1992-03-18
EP0420894A1 (de) 1991-04-10

Similar Documents

Publication Publication Date Title
Markert et al. Lactate dehydrogenase isozymes of the flatfish, Pleuronectiformes: kinetic, molecular and immunochemical analysis
RU94030817A (ru) Днк, белок,вектор, клетка, способ получения продукта
DE3586189D1 (de) Menge und verfahren zur herstellung rekombinierter erzeugnisse in grosser ausbeute.
IL72499A0 (en) Detection of bacteria by nucleic acid hybridization
Seki et al. A hydrophobic transmembrane segment at the carboxyl terminus of Thy-1
FI883708A0 (fi) Menetelmä emäksisten proteiinien eristämiseksi proteiiniseoksista, jotka sisältävät tällaisia emäksisiä proteiineja
DE68923419D1 (de) Methode zur Züchtung von Bakterien.
FR2619380B1 (fr) Procede de recuperation de l-amino-acides de liqueurs de fermentation les contenant
CA2066370A1 (en) Yield when disulfide-bonded proteins are secreted
IT8449018A1 (it) Procedimento di concentrazione di succhi fermentati per osmosi inversa
Lindqvist et al. Resistance of Escherichia coli to Penicillins VII. Purification and Characterization of a Penicillinase Mediated by the R Factor R1
DE69333491D1 (en) Protease-resistente analoge des thrombomodulin
DE68919360T2 (de) Verfahren zur reinigung hochreiner heparinase in grosser menge.
FI880660A (fi) Metod foer minskande av akrylamidhalten i vatten-i-olja-emulsioner innehaollande akrylamidpolymerer genom anvaendning av amidas.
IT8323805A0 (it) Processo per la igienizzazione di fanghi biologici.
Ramsey et al. Stable messenger ribonucleic acid and germination of Myxococcus xanthus microcysts
Kučera et al. The mechanism of pathogenicity of Pseudomonas aeruginosa: V. Isolation and properties of the proteinases toxic for larvae of the greater wax moth Galleria mellonella L
JPS57140727A (en) Novel alpha-amylase inhibitor substance
DE3788170D1 (de) Methode zur Reinigung und Isolierung von zwei ionischen Formen von Somatomedin.
ATE204580T1 (de) Verfahren zur gewinnung von cephalexin
DE68908274T2 (de) Verfahren zur Reinigung von Salzsäure.
SU575353A1 (ru) Способ получени карбоксипептидазы
DE3786947T2 (de) Mikrobiologisches Verfahren zur Gewinnung eines Proteins durch Züchtung eines mutanten Bakterienstammes.
IT1136578B (it) Processo per la produzione di l-alanina usando microorganismi immobilizzati
JPH04258288A (ja) ウミホタル・ルシフェラーゼの製造法

Legal Events

Date Code Title Description
8364 No opposition during term of opposition