DE60334029D1 - Hochempfindliche assays für die pathogendetektion unterverwendung der metallverstärkten fluoreszenz - Google Patents
Hochempfindliche assays für die pathogendetektion unterverwendung der metallverstärkten fluoreszenzInfo
- Publication number
- DE60334029D1 DE60334029D1 DE60334029T DE60334029T DE60334029D1 DE 60334029 D1 DE60334029 D1 DE 60334029D1 DE 60334029 T DE60334029 T DE 60334029T DE 60334029 T DE60334029 T DE 60334029T DE 60334029 D1 DE60334029 D1 DE 60334029D1
- Authority
- DE
- Germany
- Prior art keywords
- dna sequence
- target pathogen
- immobilized
- sequence probe
- capture dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000001717 pathogenic effect Effects 0.000 title abstract 8
- 238000003556 assay Methods 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 12
- 239000000523 sample Substances 0.000 abstract 9
- 244000052769 pathogen Species 0.000 abstract 7
- 230000001678 irradiating effect Effects 0.000 abstract 3
- 239000002923 metal particle Substances 0.000 abstract 3
- 230000000295 complement effect Effects 0.000 abstract 2
- 238000003149 assay kit Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US42926302P | 2002-11-26 | 2002-11-26 | |
PCT/US2003/038163 WO2004059279A2 (en) | 2002-11-26 | 2003-11-26 | High-sensitivity assays for pathogen detection using metal-enhanced fluorescence |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60334029D1 true DE60334029D1 (de) | 2010-10-14 |
Family
ID=32681929
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60334029T Expired - Lifetime DE60334029D1 (de) | 2002-11-26 | 2003-11-26 | Hochempfindliche assays für die pathogendetektion unterverwendung der metallverstärkten fluoreszenz |
Country Status (6)
Country | Link |
---|---|
US (2) | US8114598B2 (de) |
EP (2) | EP2248912B8 (de) |
AT (1) | ATE479775T1 (de) |
AU (1) | AU2003296014B2 (de) |
DE (1) | DE60334029D1 (de) |
WO (1) | WO2004059279A2 (de) |
Families Citing this family (79)
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US20030211488A1 (en) | 2002-05-07 | 2003-11-13 | Northwestern University | Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection |
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AU2003296014B2 (en) | 2002-11-26 | 2010-06-03 | University Of Maryland Biotechnology Institute | High-sensitivity assays for pathogen detection using metal-enhanced fluorescence |
CA2834041C (en) | 2003-12-31 | 2017-05-16 | President And Fellows Of Harvard College | Assay device and method |
US7133129B2 (en) * | 2004-05-12 | 2006-11-07 | General Electric Company | Cargo inspection apparatus having a nanoparticle film and method of use thereof |
JP4417215B2 (ja) * | 2004-09-27 | 2010-02-17 | 株式会社日立製作所 | 生体分子相互作用測定装置 |
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US7585664B2 (en) * | 2004-10-14 | 2009-09-08 | The Hong Kong University Of Science And Technology | Integrated circuit optical detector for biological detection |
US7583379B2 (en) | 2005-07-28 | 2009-09-01 | University Of Georgia Research Foundation | Surface enhanced raman spectroscopy (SERS) systems and methods of use thereof |
US7738096B2 (en) | 2004-10-21 | 2010-06-15 | University Of Georgia Research Foundation, Inc. | Surface enhanced Raman spectroscopy (SERS) systems, substrates, fabrication thereof, and methods of use thereof |
US7656525B2 (en) * | 2004-10-21 | 2010-02-02 | University Of Georgia Research Foundation, Inc. | Fiber optic SERS sensor systems and SERS probes |
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WO2006137945A2 (en) * | 2004-11-19 | 2006-12-28 | University Of Maryland Biotechnology Institute | Microwave accelerated assays |
US7485471B1 (en) * | 2004-12-17 | 2009-02-03 | Intel Corporation | Detection of enhanced multiplex signals by surface enhanced Raman spectroscopy |
US8886464B2 (en) | 2005-01-03 | 2014-11-11 | University Of Maryland, Baltimore County | Microwave-accelerated metal-enhanced detection method |
WO2007084141A2 (en) * | 2005-01-24 | 2007-07-26 | Sri International | Surface-enhanced lanthanide chelates |
US7940387B2 (en) * | 2005-03-15 | 2011-05-10 | Univeristy Of Georgia Research Foundation, Inc. | Surface enhanced Raman spectroscopy (SERS) systems for the detection of viruses and methods of use thereof |
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US7397558B2 (en) | 2005-03-17 | 2008-07-08 | Hewlett-Packard Development Company, L.P. | Ordered array of nanoparticles for efficient nanoenhanced Raman scattering detection and methods of forming the same |
US7292334B1 (en) | 2005-03-25 | 2007-11-06 | Hewlett-Packard Development Company, L.P. | Binary arrays of nanoparticles for nano-enhanced Raman scattering molecular sensors |
JP5117679B2 (ja) * | 2005-04-27 | 2013-01-16 | 株式会社リコー | 多光子吸収材料を用いた色素材料、色素材料の製造方法、多光子吸収反応材料、多光子吸収反応材料の反応生成物、多光子吸収反応助剤、および色素溶液 |
EP1915610A4 (de) * | 2005-06-15 | 2010-05-19 | Univ Maryland Biotech Inst | Bioassays mit plasmonischer streuung aus edelmetallnanostrukturen |
US7939333B2 (en) * | 2005-06-17 | 2011-05-10 | University Of Maryland, Baltimore County | Metal enhanced fluorescence-based sensing methods |
ATE525655T1 (de) * | 2005-08-02 | 2011-10-15 | Univ Maryland | Nanostrukturen zur polarisierten abbildung und rezeptor-/liganden-quantifizierung: überschreitung der diffraktionsgrenze für abbildungen |
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EP2504687B1 (de) | 2009-11-25 | 2017-04-05 | University of Maryland, Baltimore County | System zur detektion von metallverstärkter fluoreszenz aus metallischen nanoburger-strukturen |
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US11561180B2 (en) | 2015-07-22 | 2023-01-24 | University Of Maryland, Baltimore County | Hydrophilic coatings of plasmonic metals to enable low volume metal-enhanced fluorescence |
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-
2003
- 2003-11-26 AU AU2003296014A patent/AU2003296014B2/en not_active Ceased
- 2003-11-26 DE DE60334029T patent/DE60334029D1/de not_active Expired - Lifetime
- 2003-11-26 US US10/536,502 patent/US8114598B2/en active Active
- 2003-11-26 EP EP10174779.8A patent/EP2248912B8/de not_active Expired - Lifetime
- 2003-11-26 EP EP03787232A patent/EP1583948B1/de not_active Expired - Lifetime
- 2003-11-26 WO PCT/US2003/038163 patent/WO2004059279A2/en not_active Application Discontinuation
- 2003-11-26 AT AT03787232T patent/ATE479775T1/de not_active IP Right Cessation
-
2012
- 2012-02-14 US US13/372,624 patent/US9170197B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP2248912B1 (de) | 2013-03-06 |
EP1583948A4 (de) | 2006-05-10 |
WO2004059279A3 (en) | 2005-10-06 |
ATE479775T1 (de) | 2010-09-15 |
EP1583948B1 (de) | 2010-09-01 |
US20120142552A1 (en) | 2012-06-07 |
US8114598B2 (en) | 2012-02-14 |
US9170197B2 (en) | 2015-10-27 |
AU2003296014A1 (en) | 2004-07-22 |
EP2248912A1 (de) | 2010-11-10 |
EP1583948A2 (de) | 2005-10-12 |
WO2004059279A8 (en) | 2013-03-21 |
AU2003296014B2 (en) | 2010-06-03 |
US20060147927A1 (en) | 2006-07-06 |
WO2004059279A2 (en) | 2004-07-15 |
EP2248912B8 (de) | 2013-04-17 |
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