DE60231210D1 - Verfahren zur herstelllung von menschlichem thrombin mittels genmodifikationstechnik - Google Patents

Verfahren zur herstelllung von menschlichem thrombin mittels genmodifikationstechnik

Info

Publication number
DE60231210D1
DE60231210D1 DE60231210T DE60231210T DE60231210D1 DE 60231210 D1 DE60231210 D1 DE 60231210D1 DE 60231210 T DE60231210 T DE 60231210T DE 60231210 T DE60231210 T DE 60231210T DE 60231210 D1 DE60231210 D1 DE 60231210D1
Authority
DE
Germany
Prior art keywords
human thrombin
prethrombin
human
gene modification
thrombin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60231210T
Other languages
English (en)
Inventor
Hiroshi Yonemura
Takayuki Imamura
Hiroshi Nakatake
Kenji Soejima
Chikateru Nozaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemo Sero Therapeutic Research Institute Kaketsuken
Original Assignee
Chemo Sero Therapeutic Research Institute Kaketsuken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemo Sero Therapeutic Research Institute Kaketsuken filed Critical Chemo Sero Therapeutic Research Institute Kaketsuken
Application granted granted Critical
Publication of DE60231210D1 publication Critical patent/DE60231210D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Diabetes (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE60231210T 2001-07-06 2002-07-04 Verfahren zur herstelllung von menschlichem thrombin mittels genmodifikationstechnik Expired - Lifetime DE60231210D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001206919 2001-07-06
PCT/JP2002/006771 WO2003004641A1 (fr) 2001-07-06 2002-07-04 Procede relatif a l'elaboration de thrombine humaine par modification genique

Publications (1)

Publication Number Publication Date
DE60231210D1 true DE60231210D1 (de) 2009-04-02

Family

ID=19042979

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60231210T Expired - Lifetime DE60231210D1 (de) 2001-07-06 2002-07-04 Verfahren zur herstelllung von menschlichem thrombin mittels genmodifikationstechnik

Country Status (9)

Country Link
US (1) US7635577B2 (de)
EP (1) EP1405910B1 (de)
JP (1) JP4227012B2 (de)
KR (2) KR100983878B1 (de)
CN (1) CN100347297C (de)
AT (1) ATE423199T1 (de)
CA (1) CA2452768C (de)
DE (1) DE60231210D1 (de)
WO (1) WO2003004641A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040219140A1 (en) * 2001-02-22 2004-11-04 Reiner Class Compositions and methods for preventing platelet aggregation
WO2005010178A1 (ja) 2003-07-29 2005-02-03 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute 組換えフィブリノゲン高産生細胞の作製方法及び高産生細胞
GB0324044D0 (en) 2003-10-14 2003-11-19 Astrazeneca Ab Protein
DE602004026759D1 (de) 2003-10-24 2010-06-02 Chemo Sero Therapeut Res Inst Neue rekombinante tierzellen mit hoher proteinproduktion, verfahren zur konstruktion davon sowie verfahren zur massenproduktion von protein unter verwendung davon
AU2005245506A1 (en) * 2004-05-24 2005-12-01 Panacos Pharmaceuticals, Inc. Disruption of the processing of the viral capsid-spacer peptide 1 protein
CN101198696B (zh) 2005-04-13 2014-02-26 阿斯利康(瑞典)有限公司 包含产生需要γ-羧化的蛋白质用的载体的宿主细胞
WO2007040162A1 (ja) 2005-09-30 2007-04-12 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute 培養細胞による組換えヒトトロンビンの製造方法
EP1996186A4 (de) * 2006-03-06 2009-07-22 Humagene Inc Verfahren zur herstellung von rekombinantem menschlichen thrombin und fibrinogen
US8206967B2 (en) * 2007-07-06 2012-06-26 Medimmune Limited Method for production of recombinant human thrombin
CA2721335A1 (en) 2008-04-16 2009-10-22 Ryoichi Kawamura Process for preparing bioabsorbable sheet preparation holding thrombin
WO2014103608A1 (ja) 2012-12-25 2014-07-03 一般財団法人化学及血清療法研究所 HPV/HBsキメラタンパク質を有効成分とするHPV感染症及び／又はＢ型肝炎用ワクチン
JP6216647B2 (ja) 2013-03-15 2017-10-18 シスメックス株式会社 組換え型プロトロンビンの製造方法およびその利用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2824434B2 (ja) 1989-11-28 1998-11-11 財団法人化学及血清療法研究所 新規発現ベクター
US5278144A (en) 1990-09-04 1994-01-11 Cor Therapeutics, Inc. Antithrombosis agents
US5583107A (en) 1990-09-04 1996-12-10 Cor Therapeutics, Inc. Agents affecting thrombosis and hemostasis
CA2078721A1 (en) * 1991-09-24 1993-03-25 Hiroshi Yonemura Process for preparing human coagulation factor viii protein complex
JP3168087B2 (ja) * 1993-01-11 2001-05-21 株式会社ヤスヰ 洗車方法及びその装置
AT404357B (de) 1995-06-13 1998-11-25 Immuno Ag Prothrombin-derivate
WO1999058699A2 (en) 1998-05-14 1999-11-18 Battelle Memorial Institute Transgenic plant-derived human blood coagulation factors
EP1196610A2 (de) 1999-07-09 2002-04-17 Cohesion Technologies, Inc. Ecarin polypeptide, dafür kodierende polynukleotide und vefahren zur verwendung davon
CN1298017A (zh) * 1999-11-29 2001-06-06 中国科学技术大学 一种快速活化凝血酶原的方法

Also Published As

Publication number Publication date
JPWO2003004641A1 (ja) 2004-10-28
EP1405910B1 (de) 2009-02-18
KR20090087136A (ko) 2009-08-14
CN1551917A (zh) 2004-12-01
KR100983878B1 (ko) 2010-09-27
WO2003004641A1 (fr) 2003-01-16
US7635577B2 (en) 2009-12-22
EP1405910A1 (de) 2004-04-07
CN100347297C (zh) 2007-11-07
EP1405910A4 (de) 2005-02-02
KR20040032147A (ko) 2004-04-14
CA2452768A1 (en) 2003-01-16
JP4227012B2 (ja) 2009-02-18
CA2452768C (en) 2011-06-07
ATE423199T1 (de) 2009-03-15
US20040197858A1 (en) 2004-10-07

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