DE60226367D1 - Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle - Google Patents

Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle

Info

Publication number
DE60226367D1
DE60226367D1 DE60226367T DE60226367T DE60226367D1 DE 60226367 D1 DE60226367 D1 DE 60226367D1 DE 60226367 T DE60226367 T DE 60226367T DE 60226367 T DE60226367 T DE 60226367T DE 60226367 D1 DE60226367 D1 DE 60226367D1
Authority
DE
Germany
Prior art keywords
control
nucleic acid
target nucleic
determination
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60226367T
Other languages
English (en)
Inventor
Stephan Dr Jaeger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of DE60226367D1 publication Critical patent/DE60226367D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
DE60226367T 2001-03-02 2002-02-27 Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle Expired - Lifetime DE60226367D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP01105172A EP1236804A1 (de) 2001-03-02 2001-03-02 Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle

Publications (1)

Publication Number Publication Date
DE60226367D1 true DE60226367D1 (de) 2008-06-19

Family

ID=8176662

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60226367T Expired - Lifetime DE60226367D1 (de) 2001-03-02 2002-02-27 Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle

Country Status (8)

Country Link
US (3) US7192745B2 (de)
EP (1) EP1236804A1 (de)
JP (1) JP3787101B2 (de)
CN (1) CN100457919C (de)
AT (1) ATE394506T1 (de)
CA (1) CA2371672C (de)
DE (1) DE60226367D1 (de)
HK (1) HK1052030A1 (de)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8275554B2 (en) * 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
WO2004083443A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
EP1469083A1 (de) * 2003-04-17 2004-10-20 Centre National De La Recherche Scientifique (Cnrs) Kalibrierungsverfahren für reverse Transkriptionen unter Verwendung einer synthetischen mRNA als interne Kontrolle
US8536661B1 (en) 2004-06-25 2013-09-17 University Of Hawaii Biosensor chip sensor protection methods
JP2006217897A (ja) * 2005-02-14 2006-08-24 Canon Inc コントロールブローブ用塩基配列及びその設計方法
AU2006223223B2 (en) * 2005-03-10 2012-04-12 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
WO2006102470A2 (en) * 2005-03-22 2006-09-28 Posnick, Jeffrey, C. Facial implant
US8067208B2 (en) * 2005-06-30 2011-11-29 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using multidimensional probe analysis
US7368246B2 (en) * 2005-09-15 2008-05-06 Primera Biosystems, Inc. Quantitative gene expression profiling
US20060287654A1 (en) * 2006-08-11 2006-12-21 Jeffrey Posnick Implant securing device and method
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2092322B1 (de) 2006-12-14 2016-02-17 Life Technologies Corporation Verfahren und vorrichtungen zur messung von analyten unter verwendung von grossflächigen fet-arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2982437B1 (de) 2008-06-25 2017-12-06 Life Technologies Corporation Verfahren und vorrichtung zur messung von analyten mithilfe grossskaliger fet-arrays
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100129902A1 (en) 2008-11-24 2010-05-27 Erhard Ralf Schoenbrunner Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR)
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
CN106932456B (zh) 2010-06-30 2020-02-21 生命科技公司 用于测试isfet阵列的方法和装置
WO2012003363A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
US8487790B2 (en) 2010-06-30 2013-07-16 Life Technologies Corporation Chemical detection circuit including a serializer circuit
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
EP2589065B1 (de) 2010-07-03 2015-08-19 Life Technologies Corporation Chemisch empfindlicher sensor mit leicht dotierten abflüssen
US8609340B2 (en) * 2010-07-29 2013-12-17 Roche Molecular Systems, Inc. Control nucleic acids for multiple parameters
US8877464B2 (en) * 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
EP2617061B1 (de) 2010-09-15 2021-06-30 Life Technologies Corporation Verfahren und vorrichtung zur analytmessung
US8685324B2 (en) 2010-09-24 2014-04-01 Life Technologies Corporation Matched pair transistor circuits
EP2678664B1 (de) 2011-02-24 2019-08-07 Gen-Probe Incorporated Systeme und verfahren zur unterscheidung optischer signale mit verschiedenen modulationsfrequenzen bei einem optischen signaldetektor
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
EP2722397B1 (de) * 2012-10-18 2017-12-13 F. Hoffmann-La Roche AG Test mit zwei Sonden zur Erkennung von heterogenen Amplikonpopulationen
US11274998B2 (en) 2012-12-26 2022-03-15 Ventana Medical Systems, Inc. Specimen processing systems and methods for holding slides
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
CN105264366B (zh) 2013-03-15 2019-04-16 生命科技公司 具有一致传感器表面区域的化学传感器
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
EP2972279B1 (de) 2013-03-15 2021-10-06 Life Technologies Corporation Chemischer sensor mit stetigen sensoroberflächen
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
US20140264471A1 (en) 2013-03-15 2014-09-18 Life Technologies Corporation Chemical device with thin conductive element
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
CN107250784B (zh) 2014-12-18 2020-10-23 生命科技公司 具有发送器配置的高数据率集成电路
EP3234575B1 (de) 2014-12-18 2023-01-25 Life Technologies Corporation Vorrichtung zur messung von analyten unter verwendung von grossflächigen fet-arrays
CN110004216A (zh) * 2019-04-16 2019-07-12 艾美汉信疫苗（大连）有限公司 应用荧光实时定量pcr检测重组汉逊酵母发酵表达速率的方法
JP7304631B2 (ja) * 2020-03-25 2023-07-07 国立研究開発法人農業・食品産業技術総合研究機構 ポスピウイロイドの解析用ポジティブコントロール及びその利用
CN114891868A (zh) * 2022-05-31 2022-08-12 广州市金圻睿生物科技有限责任公司 一种基于ngs平台的微生物定量方法及试剂盒

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE3739366A1 (de) 1987-04-10 1988-10-27 Boehringer Mannheim Gmbh Desaza-purin-nucleosid-derivate, verfahren zu deren herstellung sowie deren verwendung bei der nucleinsaeure-sequenzierung sowie als antivirale mittel
US6211158B1 (en) 1987-04-10 2001-04-03 Roche Diagnostics Gmbh Desazapurine-nucleotide derivatives, processes for the preparation thereof, pharmaceutical compositions containing them and the use thereof for nucleic acid sequencing and as antiviral agents
DE3813278A1 (de) 1988-01-12 1989-07-20 Boehringer Mannheim Gmbh Verfahren zum nachweis von nukleinsaeuren
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
DE4140463A1 (de) 1991-12-09 1993-06-17 Boehringer Mannheim Gmbh 2'-desoxy-isoguanosin, dessen isostere analoge sowie anwendung derselben
ZA936015B (en) * 1992-08-24 1994-03-10 Akzo Nv Elimination of false negatives in nuleic acid detection.
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
US5837501A (en) * 1993-07-09 1998-11-17 Akzo Nobel N.V. Nucleic acid quantitation by co-amplification of target with multiple internal controls
DE4415370A1 (de) 1994-05-02 1995-11-09 Hoechst Ag Modifizierte Oligonukleotide, deren Herstellung sowie deren Verwendung
US5837442A (en) * 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
US6849400B1 (en) * 1997-07-23 2005-02-01 Gen-Probe Incorporated Methods for detecting and measuring spliced nucleic acids
US6395470B2 (en) * 1997-10-31 2002-05-28 Cenetron Diagnostics, Llc Method for monitoring nucleic acid assays using synthetic internal controls with reversed nucleotide sequences
US6135943A (en) 1998-08-07 2000-10-24 Cardiac Assist Technologies, Inc. Non-invasive flow indicator for a rotary blood pump
IT1303767B1 (it) 1998-11-17 2001-02-23 San Raffaele Centro Fond Metodo di quantificazione di acidi nucleici.
CN1094154C (zh) * 1999-02-12 2002-11-13 中山医科大学达安基因股份有限公司 一种荧光定量聚合酶链式反应方法及其试剂盒
US7422850B2 (en) 2000-05-19 2008-09-09 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids

Also Published As

Publication number Publication date
CN1386865A (zh) 2002-12-25
US7192745B2 (en) 2007-03-20
EP1236804A1 (de) 2002-09-04
US20030054372A1 (en) 2003-03-20
US7462709B2 (en) 2008-12-09
US20030165982A1 (en) 2003-09-04
CN100457919C (zh) 2009-02-04
US7183084B2 (en) 2007-02-27
CA2371672A1 (en) 2002-09-02
JP3787101B2 (ja) 2006-06-21
JP2002335981A (ja) 2002-11-26
US20070117137A1 (en) 2007-05-24
HK1052030A1 (en) 2003-08-29
ATE394506T1 (de) 2008-05-15
CA2371672C (en) 2009-04-14

Similar Documents

Publication Publication Date Title
DE60226367D1 (de) Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle
DE60129869D1 (de) Methode zur Quantifizierung von Nukleinsäuren in Echtzeit mittels Effizienzkorrektur
ATE282716T1 (de) Verfahren zur amplifikation von nukleinsäuresequenzen
ATE542917T1 (de) Zusammensetzungen, verfahren und kits zur bestimmung des vorhandenseins von trichomonas vaginalis in einer testprobe
DE69837913D1 (de) Verfahren zur vervielfältigung von nukleinsäure
DE69333891D1 (de) Verfahren zur Nukleinsäurenamplifizierung, Zusammensetzung und Kit
ATE428003T1 (de) Oligonukleotidprimer zur effizienten detektion von hepatitis c virus (hcv) sowie verwendungsverfahren
ATE548464T1 (de) Detektion von nukleinsäuren durch typenspezifische hybrid-capture methode
ATE399880T1 (de) Ein in-vitro-verfahren zur herstellung einer dna- bibliothek
ATE552340T1 (de) Hepatitis c virus genotypsequenzen sowie ihre verwendung als behandlungs- und nachweismitteln
ATE191513T1 (de) Verfahren zur amplifikation von nukleinsäuren
DE60009323D1 (de) Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
DE60323067D1 (de) Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination
ATE310718T1 (de) Verfahren zur herstellung von (meth)acrylsäureanhydrid
ATE340258T1 (de) Verfahren zur herstellung von nukleinsäuren bestehend aus stochastisch kombinierten anteilen von ausgangsnukleinsäuren
DE60132924D1 (de) Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2)
DE60326327D1 (de) Verfahren und zusammensetzungen zur identifikation und charakterisierung von hepatitis c
ATE539167T1 (de) Verfahren zur amplifikation von rna-sequenzen
ATE445023T1 (de) Auf nukleinsäurenamplifizierung basierendes verfahren zur bestimmung von methylierungsprofil und reagenzien dafür
HK1066222A1 (en) Mannitol and glucitol derivatives
DE602004030036D1 (de) Multiplexanalyse von Nukleinsäuren zur Bestimmung von Genotypen des humanen Papillomavirus
ATE214096T1 (de) Verfahren zur spezifischen klonierung von nukleinsäuren
ATE487798T1 (de) Verfahren zur identifizierung biologisch aktiver strukturen mikrobieller erreger
ATE278699T1 (de) Verfahren zur aufreinigung von nukleinsäuren sowie anionenaustauscher zur durchführung dieses verfahrens
ATE456669T1 (de) Verfahren zur quantifizierung von nukleinsäuren

Legal Events

Date Code Title Description
8364 No opposition during term of opposition