DE60214400T8 - Verfahren zur proteinproduktion in pflanzen - Google Patents

Verfahren zur proteinproduktion in pflanzen Download PDF

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Publication number
DE60214400T8
DE60214400T8 DE60214400T DE60214400T DE60214400T8 DE 60214400 T8 DE60214400 T8 DE 60214400T8 DE 60214400 T DE60214400 T DE 60214400T DE 60214400 T DE60214400 T DE 60214400T DE 60214400 T8 DE60214400 T8 DE 60214400T8
Authority
DE
Germany
Prior art keywords
protein
polypeptide
interest
plant
fusion protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE60214400T
Other languages
English (en)
Other versions
DE60214400D1 (de
DE60214400T2 (de
Inventor
Yurii Moscow DOROKHOV
Eugene Moscow SKURAT
Victor Klimyuk
Yuri Gleba
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Icon Genetics AG
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Icon Genetics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Icon Genetics AG filed Critical Icon Genetics AG
Publication of DE60214400D1 publication Critical patent/DE60214400D1/de
Application granted granted Critical
Publication of DE60214400T2 publication Critical patent/DE60214400T2/de
Publication of DE60214400T8 publication Critical patent/DE60214400T8/de
Active legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DE60214400T 2001-09-04 2002-08-28 Verfahren zur proteinproduktion in pflanzen Active DE60214400T8 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10143205 2001-09-04
DE10143205A DE10143205A1 (de) 2001-09-04 2001-09-04 Verfahren zur Proteinproduktion in Pflanzen
PCT/EP2002/009605 WO2003020938A2 (en) 2001-09-04 2002-08-28 Method of protein production in plants

Publications (3)

Publication Number Publication Date
DE60214400D1 DE60214400D1 (de) 2006-10-12
DE60214400T2 DE60214400T2 (de) 2007-10-11
DE60214400T8 true DE60214400T8 (de) 2008-02-07

Family

ID=7697601

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10143205A Withdrawn DE10143205A1 (de) 2001-09-04 2001-09-04 Verfahren zur Proteinproduktion in Pflanzen
DE60214400T Active DE60214400T8 (de) 2001-09-04 2002-08-28 Verfahren zur proteinproduktion in pflanzen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10143205A Withdrawn DE10143205A1 (de) 2001-09-04 2001-09-04 Verfahren zur Proteinproduktion in Pflanzen

Country Status (10)

Country Link
US (1) US20050015830A1 (de)
EP (1) EP1423524B1 (de)
JP (1) JP4401772B2 (de)
AT (1) ATE338138T1 (de)
AU (1) AU2002331090B2 (de)
CA (1) CA2455882C (de)
DE (2) DE10143205A1 (de)
IL (2) IL160338A0 (de)
MX (1) MXPA04001871A (de)
WO (1) WO2003020938A2 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10049587A1 (de) 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10132780A1 (de) 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
DE10143237A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
DE10143238A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
DE10325814A1 (de) * 2003-06-06 2004-12-23 Icon Genetics Ag Sichere Erzeugung eines gewünschten Produkts in hybriden Samen
US8507277B2 (en) * 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US20090208478A1 (en) * 2003-10-24 2009-08-20 Gencia Corporation Transducible polypeptides for modifying metabolism
US8133733B2 (en) * 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
US8039587B2 (en) 2003-10-24 2011-10-18 Gencia Corporation Methods and compositions for delivering polynucleotides
US8062891B2 (en) * 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
BRPI0710689A2 (pt) 2006-04-21 2012-02-22 Dow Agrosciences Llc método para preparação de uma vacina de aiv feita de planta e a referida vacina
FI20060533L (fi) * 2006-06-01 2007-12-02 Timo Kalevi Korpela Parannettu tuberkuloosirokoteproteiinien ilmentäminen kasveissa
EP1897953A1 (de) * 2006-09-06 2008-03-12 Icon Genetics GmbH Replicon von Potexviren stammend
EP2405009B1 (de) 2007-03-29 2016-08-03 Futuragene Israel Ltd. Transgene Pflanzen, die lösliche Zellwandpolysaccharide enthalten
US20100212050A1 (en) * 2007-03-29 2010-08-19 Cbd Technologies Ltd. Plants over-expressing pme
TWI620816B (zh) * 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
EP2584042A1 (de) 2011-10-17 2013-04-24 Nomad Bioscience GmbH Herstellung, Lagerung und Verwendung von zellwandabbauenden Enzymen
CA2904472A1 (en) 2013-03-22 2014-09-25 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Kits comprising plus-sense single stranded rna viral vectors and methods for producing polypeptides using the kits
EP3456829A1 (de) 2017-09-18 2019-03-20 Nomad Bioscience GmbH Verfahren zur verbesserung der potexviralen vektorstabilität
CN113015743A (zh) 2018-11-15 2021-06-22 三菱化学株式会社 改变型激活素a
WO2020124128A1 (en) * 2018-12-20 2020-06-25 Agriculture Victoria Services Pty Ltd Method of protein extraction from cannabis plant material

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US6174700B1 (en) * 1988-07-08 2001-01-16 University Of British Columbia Purification of a polypeptide compound having a polysaccharide binding domain by affinity phase separation
FR2649518B1 (fr) * 1989-07-07 1991-10-18 Bioprobe Systems Sa Procede et dispositif de marquage crypte de haute securite pour la protection d'objets de valeur
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5474925A (en) * 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber
US5576198A (en) * 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US6037525A (en) * 1996-08-01 2000-03-14 North Carolina State University Method for reducing expression variability of transgenes in plant cells
US5981835A (en) * 1996-10-17 1999-11-09 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
NZ504510A (en) * 1997-11-18 2002-10-25 Pioneer Hi Bred Int Methods and compositions for increasing efficiency of excision of a viral replicon from T-DNA that is transferred to a plant by agroinfection
CA2306184C (en) * 1997-11-18 2007-05-15 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
AU6455499A (en) * 1998-10-23 2000-11-21 Cbd Technologies Ltd. Expression of a Mannan Binding Domain to Alter Plant Morphology
WO2001081600A2 (en) * 2000-04-20 2001-11-01 Btg International Limited Transgenic plants
AU2001257300A1 (en) * 2000-04-26 2001-11-07 Monsanto Technology Llc Method for the transformation of plant cell plastids
DE10049587A1 (de) * 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) * 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10101276A1 (de) * 2001-01-12 2002-07-18 Icon Genetics Ag Verfahren und Vektoren zur Transformation von Plastiden
DE10102389A1 (de) * 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
DE10109354A1 (de) * 2001-02-27 2002-09-05 Icon Genetics Ag Rekombinante virale Schaltersysteme
DE10114209A1 (de) * 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) * 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
DE10121283B4 (de) * 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10129010A1 (de) * 2001-06-13 2002-12-19 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10131680A1 (de) * 2001-06-29 2003-01-23 Voith Paper Patent Gmbh Auftragsvorrichtung
DE10132780A1 (de) * 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
DE10143237A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
KR100571910B1 (ko) * 2003-07-15 2006-04-17 삼성전자주식회사 점대점 네트워크를 통해 통신하는 무선통신망에서ＱｏＳ를 제공하는 방법 및 ＱｏＳ를 제공하는 무선통신시스템

Also Published As

Publication number Publication date
ATE338138T1 (de) 2006-09-15
CA2455882A1 (en) 2003-03-13
CA2455882C (en) 2011-04-05
US20050015830A1 (en) 2005-01-20
IL160338A (en) 2009-12-24
IL160338A0 (en) 2004-07-25
JP4401772B2 (ja) 2010-01-20
WO2003020938A2 (en) 2003-03-13
MXPA04001871A (es) 2004-07-23
DE10143205A1 (de) 2003-03-20
EP1423524B1 (de) 2006-08-30
EP1423524A2 (de) 2004-06-02
DE60214400D1 (de) 2006-10-12
WO2003020938A3 (en) 2003-10-02
JP2005501558A (ja) 2005-01-20
DE60214400T2 (de) 2007-10-11
AU2002331090B2 (en) 2007-07-12

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8327 Change in the person/name/address of the patent owner

Owner name: ICON GENETICS GMBH, 80539 MUENCHEN, DE

8364 No opposition during term of opposition