DE60214400D1 - Verfahren zur proteinproduktion in pflanzen - Google Patents

Verfahren zur proteinproduktion in pflanzen

Info

Publication number
DE60214400D1
DE60214400D1 DE60214400T DE60214400T DE60214400D1 DE 60214400 D1 DE60214400 D1 DE 60214400D1 DE 60214400 T DE60214400 T DE 60214400T DE 60214400 T DE60214400 T DE 60214400T DE 60214400 D1 DE60214400 D1 DE 60214400D1
Authority
DE
Germany
Prior art keywords
protein
polypeptide
interest
plant
fusion protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60214400T
Other languages
English (en)
Other versions
DE60214400T2 (de
DE60214400T8 (de
Inventor
Yurii Dorokhov
Eugene Skurat
Victor Klimyuk
Yuri Gleba
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Icon Genetics AG
Original Assignee
Icon Genetics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Icon Genetics AG filed Critical Icon Genetics AG
Publication of DE60214400D1 publication Critical patent/DE60214400D1/de
Publication of DE60214400T2 publication Critical patent/DE60214400T2/de
Application granted granted Critical
Publication of DE60214400T8 publication Critical patent/DE60214400T8/de
Active legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DE60214400T 2001-09-04 2002-08-28 Verfahren zur proteinproduktion in pflanzen Active DE60214400T8 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10143205A DE10143205A1 (de) 2001-09-04 2001-09-04 Verfahren zur Proteinproduktion in Pflanzen
DE10143205 2001-09-04
PCT/EP2002/009605 WO2003020938A2 (en) 2001-09-04 2002-08-28 Method of protein production in plants

Publications (3)

Publication Number Publication Date
DE60214400D1 true DE60214400D1 (de) 2006-10-12
DE60214400T2 DE60214400T2 (de) 2007-10-11
DE60214400T8 DE60214400T8 (de) 2008-02-07

Family

ID=7697601

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10143205A Withdrawn DE10143205A1 (de) 2001-09-04 2001-09-04 Verfahren zur Proteinproduktion in Pflanzen
DE60214400T Active DE60214400T8 (de) 2001-09-04 2002-08-28 Verfahren zur proteinproduktion in pflanzen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10143205A Withdrawn DE10143205A1 (de) 2001-09-04 2001-09-04 Verfahren zur Proteinproduktion in Pflanzen

Country Status (10)

Country Link
US (1) US20050015830A1 (de)
EP (1) EP1423524B1 (de)
JP (1) JP4401772B2 (de)
AT (1) ATE338138T1 (de)
AU (1) AU2002331090B2 (de)
CA (1) CA2455882C (de)
DE (2) DE10143205A1 (de)
IL (2) IL160338A0 (de)
MX (1) MXPA04001871A (de)
WO (1) WO2003020938A2 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10049587A1 (de) 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
DE10114209A1 (de) * 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10132780A1 (de) 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
DE10143238A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
DE10143237A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
DE10325814A1 (de) * 2003-06-06 2004-12-23 Icon Genetics Ag Sichere Erzeugung eines gewünschten Produkts in hybriden Samen
US20090208478A1 (en) * 2003-10-24 2009-08-20 Gencia Corporation Transducible polypeptides for modifying metabolism
US8507277B2 (en) * 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US8062891B2 (en) * 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
US8133733B2 (en) * 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
US20090123468A1 (en) * 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
ES2411962T3 (es) 2003-10-24 2013-07-09 Gencia Corporation Métodos y composiciones para suministrar polinucleótidos
KR20080109094A (ko) 2006-04-21 2008-12-16 다우 아그로사이언시즈 엘엘씨 조류 인플루엔자 백신 및 이용방법
FI20060533L (fi) * 2006-06-01 2007-12-02 Timo Kalevi Korpela Parannettu tuberkuloosirokoteproteiinien ilmentäminen kasveissa
EP1897953A1 (de) * 2006-09-06 2008-03-12 Icon Genetics GmbH Replicon von Potexviren stammend
US20100212050A1 (en) * 2007-03-29 2010-08-19 Cbd Technologies Ltd. Plants over-expressing pme
EP2140009A2 (de) 2007-03-29 2010-01-06 CBD Technologies Ltd. Transgene pflanzen mit löslichen zellwand-polysacchariden
TWI620816B (zh) * 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
EP2584042A1 (de) 2011-10-17 2013-04-24 Nomad Bioscience GmbH Herstellung, Lagerung und Verwendung von zellwandabbauenden Enzymen
EP2976428B1 (de) 2013-03-22 2019-11-27 Fraunhofer Gesellschaft zur Förderung der Angewand Kits mit einsträngigen rna-virenvektoren mit plus-polarität und verfahren zur herstellung von polypeptiden unter verwendung der kits
EP3456829A1 (de) 2017-09-18 2019-03-20 Nomad Bioscience GmbH Verfahren zur verbesserung der potexviralen vektorstabilität
EP3882262A4 (de) 2018-11-15 2022-03-30 Mitsubishi Chemical Corporation Modifiziertes activin a
US20230027592A1 (en) * 2018-12-20 2023-01-26 Agriculture Victoria Services Pty Ltd Method of Protein Extraction from Cannabis Plant Material

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6147278A (en) * 1985-10-25 2000-11-14 Monsanto Company Plant vectors
GB8626878D0 (en) * 1986-11-11 1986-12-10 Ici Plc Dna
US6174700B1 (en) * 1988-07-08 2001-01-16 University Of British Columbia Purification of a polypeptide compound having a polysaccharide binding domain by affinity phase separation
FR2649518B1 (fr) * 1989-07-07 1991-10-18 Bioprobe Systems Sa Procede et dispositif de marquage crypte de haute securite pour la protection d'objets de valeur
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5474925A (en) * 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber
US5576198A (en) * 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US6037525A (en) * 1996-08-01 2000-03-14 North Carolina State University Method for reducing expression variability of transgenes in plant cells
US5981835A (en) * 1996-10-17 1999-11-09 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
CA2306184C (en) * 1997-11-18 2007-05-15 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
DE69841438D1 (de) * 1997-11-18 2010-02-25 Pioneer Hi Bred Int Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme
AU6455499A (en) * 1998-10-23 2000-11-21 Cbd Technologies Ltd. Expression of a Mannan Binding Domain to Alter Plant Morphology
EP1276884A2 (de) * 2000-04-20 2003-01-22 Btg International Limited Transgene pflanzen
EP1276886B1 (de) * 2000-04-26 2012-11-28 Monsanto Technology LLC Verfahren zur transformation von pflanzenzellplastiden
DE10049587A1 (de) * 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) * 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10101276A1 (de) * 2001-01-12 2002-07-18 Icon Genetics Ag Verfahren und Vektoren zur Transformation von Plastiden
DE10102389A1 (de) * 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
DE10109354A1 (de) * 2001-02-27 2002-09-05 Icon Genetics Ag Rekombinante virale Schaltersysteme
DE10114209A1 (de) * 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) * 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
DE10121283B4 (de) * 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10129010A1 (de) * 2001-06-13 2002-12-19 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10131680A1 (de) * 2001-06-29 2003-01-23 Voith Paper Patent Gmbh Auftragsvorrichtung
DE10132780A1 (de) * 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
DE10143237A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
KR100571910B1 (ko) * 2003-07-15 2006-04-17 삼성전자주식회사 점대점 네트워크를 통해 통신하는 무선통신망에서ＱｏＳ를 제공하는 방법 및 ＱｏＳ를 제공하는 무선통신시스템

Also Published As

Publication number Publication date
WO2003020938A3 (en) 2003-10-02
MXPA04001871A (es) 2004-07-23
ATE338138T1 (de) 2006-09-15
WO2003020938A2 (en) 2003-03-13
IL160338A0 (en) 2004-07-25
DE60214400T2 (de) 2007-10-11
AU2002331090B2 (en) 2007-07-12
IL160338A (en) 2009-12-24
DE10143205A1 (de) 2003-03-20
JP2005501558A (ja) 2005-01-20
CA2455882C (en) 2011-04-05
DE60214400T8 (de) 2008-02-07
EP1423524A2 (de) 2004-06-02
CA2455882A1 (en) 2003-03-13
EP1423524B1 (de) 2006-08-30
JP4401772B2 (ja) 2010-01-20
US20050015830A1 (en) 2005-01-20

Similar Documents

Publication Publication Date Title
DE60214400T8 (de) Verfahren zur proteinproduktion in pflanzen
WO2001029242A3 (en) Post-translational modification of recombinant proteins produced in plants
WO2001045494A3 (en) Pyrophosphatase stress-related proteins and methods of use in plants
AU2001255250A1 (en) Cell cycle stress-related proteins and methods of use in plants
DE59710862D1 (de) Nucleinsäuremoleküle aus pflanzen codierend enzyme, die an der stärkesynthese beteiligt sind
EP1571221A3 (de) Gene, welche an der Toleranz gegenüber Umweltstress beteiligt sind
ATE490309T1 (de) Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon
DE60333201D1 (de) Verfahren zur herstellung von proteinen
ATE274577T1 (de) Stammzellen-proliferations-faktor
AU7659298A (en) Recombinant lactoferrin, methods of production from plants and uses
ATE279441T1 (de) Verfahren zur herstellung von genetisch veränderten lymphozyten vorläuferzellen von wirbeltieren und deren gebrauch zur produktion von heterologen bindeproteinen
PT1200561E (pt) Clone celular recombinante estavel, sua producao e utilizacao
DE60122562D1 (de) Nukleinsäuren, die für stichodactylidae chromoproteine kodieren
ATE533851T1 (de) Prozess zur herstellung eines plastidgerichteten proteins in pflanzenzellen
CA2088068A1 (en) Protein structure of the plant toxin gelonin
WO2001094393A3 (de) Synthetische spinnenseidenproteine und deren expression in transgenen pflanzen
HUP0003564A2 (hu) Protein kinázok és alkalmazásuk
WO2002008417A1 (fr) Procede de production d'une proteine de recombinaison
ATE541920T1 (de) Mit stress verbundene proteinphosphatase- polypeptide und verfahren zur verwendung in pflanzen
DE60234101D1 (de) Durch verstärktes wachstum charakterisierte pflanzen sowie für deren erzeugung geeignete verfahren und nukleinsäurekonstrukte
WO2003076595A3 (en) Production of insulin and insulin-like proteins in plants
ATE504603T1 (de) Verfahren zur herstellung von interessanten polypeptiden aus fusionspolypeptiden
DE60109819D1 (de) Expression von multiplen proteinen in transgenen pflanzen
WO2004113539A3 (fr) Procede de production d’une proteine recombinante d’interet et proteine produite
ATE510016T1 (de) Mit stress assoziierte transkriptionsfaktor- spezifische polypeptide und verfahren zur verwendung in pflanzen

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: ICON GENETICS GMBH, 80539 MUENCHEN, DE

8364 No opposition during term of opposition