DE60203005T2 - Synthese und ausprüfung von neuen cyaninfarbstoffen als an die kleine furche von poly(dadt)2 bindende stoffe - Google Patents
Synthese und ausprüfung von neuen cyaninfarbstoffen als an die kleine furche von poly(dadt)2 bindende stoffe Download PDFInfo
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- DE60203005T2 DE60203005T2 DE60203005T DE60203005T DE60203005T2 DE 60203005 T2 DE60203005 T2 DE 60203005T2 DE 60203005 T DE60203005 T DE 60203005T DE 60203005 T DE60203005 T DE 60203005T DE 60203005 T2 DE60203005 T2 DE 60203005T2
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- WMTPOJFMENVFHE-UHFFFAOYSA-M 3-methyl-2-[(1-methylpyridin-1-ium-4-yl)methylidene]-1,3-benzothiazole;iodide Chemical compound [I-].S1C2=CC=CC=C2N(C)C1=CC1=CC=[N+](C)C=C1 WMTPOJFMENVFHE-UHFFFAOYSA-M 0.000 description 1
- XRTJYEIMLZALBD-UHFFFAOYSA-N 4-(6-methyl-1,3-benzothiazol-2-yl)aniline Chemical compound S1C2=CC(C)=CC=C2N=C1C1=CC=C(N)C=C1 XRTJYEIMLZALBD-UHFFFAOYSA-N 0.000 description 1
- GMTJDCKSZCDPFA-UHFFFAOYSA-M 6-(1,3-benzothiazol-2-yl)-2,3-dimethyl-1,3-benzothiazol-3-ium;4-methylbenzenesulfonate Chemical compound CC1=CC=C(S([O-])(=O)=O)C=C1.C1=CC=C2SC(C=3C=C4SC(=[N+](C4=CC=3)C)C)=NC2=C1 GMTJDCKSZCDPFA-UHFFFAOYSA-M 0.000 description 1
- 241000974482 Aricia saepiolus Species 0.000 description 1
- 0 CCC1=CC*(C)(*)C(*)C1 Chemical compound CCC1=CC*(C)(*)C(*)C1 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical group O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 235000019502 Orange oil Nutrition 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- MZZINWWGSYUHGU-UHFFFAOYSA-J ToTo-1 Chemical compound [I-].[I-].[I-].[I-].C12=CC=CC=C2C(C=C2N(C3=CC=CC=C3S2)C)=CC=[N+]1CCC[N+](C)(C)CCC[N+](C)(C)CCC[N+](C1=CC=CC=C11)=CC=C1C=C1N(C)C2=CC=CC=C2S1 MZZINWWGSYUHGU-UHFFFAOYSA-J 0.000 description 1
- OKJPEAGHQZHRQV-UHFFFAOYSA-N Triiodomethane Natural products IC(I)I OKJPEAGHQZHRQV-UHFFFAOYSA-N 0.000 description 1
- FZNCGRZWXLXZSZ-CIQUZCHMSA-N Voglibose Chemical compound OCC(CO)N[C@H]1C[C@](O)(CO)[C@@H](O)[C@H](O)[C@H]1O FZNCGRZWXLXZSZ-CIQUZCHMSA-N 0.000 description 1
- 229960001413 acetanilide Drugs 0.000 description 1
- 230000010933 acylation Effects 0.000 description 1
- 238000005917 acylation reaction Methods 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 229940058303 antinematodal benzimidazole derivative Drugs 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 150000001502 aryl halides Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 150000001556 benzimidazoles Chemical class 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002983 circular dichroism Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 229940125904 compound 1 Drugs 0.000 description 1
- 229940126214 compound 3 Drugs 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005421 electrostatic potential Methods 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000000763 evoking effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 238000007478 fluorogenic assay Methods 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002496 iodine Chemical class 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 239000010502 orange oil Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 238000010651 palladium-catalyzed cross coupling reaction Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229910000073 phosphorus hydride Inorganic materials 0.000 description 1
- INAAIJLSXJJHOZ-UHFFFAOYSA-N pibenzimol Chemical compound C1CN(C)CCN1C1=CC=C(N=C(N2)C=3C=C4NC(=NC4=CC=3)C=3C=CC(O)=CC=3)C2=C1 INAAIJLSXJJHOZ-UHFFFAOYSA-N 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- JUJWROOIHBZHMG-UHFFFAOYSA-O pyridinium Chemical compound C1=CC=[NH+]C=C1 JUJWROOIHBZHMG-UHFFFAOYSA-O 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 238000006798 ring closing metathesis reaction Methods 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 125000004055 thiomethyl group Chemical group [H]SC([H])([H])* 0.000 description 1
- 230000036964 tight binding Effects 0.000 description 1
- ITHPEWAHFNDNIO-UHFFFAOYSA-N triphosphane Chemical compound PPP ITHPEWAHFNDNIO-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
- C09B23/04—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Plural Heterocyclic Compounds (AREA)
- Thermal Transfer Or Thermal Recording In General (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Polyesters Or Polycarbonates (AREA)
- Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
- Adhesives Or Adhesive Processes (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0101651 | 2001-05-10 | ||
| SE0101651A SE519116C2 (sv) | 2001-05-10 | 2001-05-10 | Syntes och utvärdering av nya cyaninfärgämnen som binder till minor groove |
| PCT/SE2002/000860 WO2002090443A1 (en) | 2001-05-10 | 2002-05-10 | Synthesis and evaluation of new cyanine dyes as minor groove of [poly(da-dt)]2 binders |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60203005D1 DE60203005D1 (de) | 2005-03-24 |
| DE60203005T2 true DE60203005T2 (de) | 2006-05-11 |
Family
ID=20284061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60203005T Expired - Fee Related DE60203005T2 (de) | 2001-05-10 | 2002-05-10 | Synthese und ausprüfung von neuen cyaninfarbstoffen als an die kleine furche von poly(dadt)2 bindende stoffe |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7378240B2 (https=) |
| EP (1) | EP1390433B1 (https=) |
| JP (1) | JP2004536900A (https=) |
| AT (1) | ATE289337T1 (https=) |
| AU (1) | AU2002303054B2 (https=) |
| CA (1) | CA2446982C (https=) |
| DE (1) | DE60203005T2 (https=) |
| SE (1) | SE519116C2 (https=) |
| WO (1) | WO2002090443A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE519116C2 (sv) | 2001-05-10 | 2003-01-14 | Lightup Technologies Ab | Syntes och utvärdering av nya cyaninfärgämnen som binder till minor groove |
| US7776567B2 (en) | 2005-03-17 | 2010-08-17 | Biotium, Inc. | Dimeric and trimeric nucleic acid dyes, and associated systems and methods |
| US7601498B2 (en) * | 2005-03-17 | 2009-10-13 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection and associated technology |
| JP5306811B2 (ja) * | 2005-05-11 | 2013-10-02 | ライフ テクノロジーズ コーポレーション | ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用 |
| US9433700B2 (en) | 2009-04-27 | 2016-09-06 | Medibeacon Inc. | Tissue sealant compositions, vascular closure devices, and uses thereof |
| US8877437B1 (en) | 2009-12-23 | 2014-11-04 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection |
| CN105765066B (zh) * | 2013-11-29 | 2018-12-14 | 宝生物工程株式会社 | 用于定量腺伴随病毒的方法 |
| CN108165045B (zh) * | 2018-01-15 | 2021-03-16 | 广东工业大学 | 核酸染料化合物及其制备方法和应用 |
| WO2023086970A2 (en) | 2021-11-12 | 2023-05-19 | Arna Genomics Us Incorporated | Methods and compositions for analyzing nucleic acids and nucleases in a body fluid as indicators of disease |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5656449A (en) * | 1995-03-06 | 1997-08-12 | Molecular Probes, Inc. | Neutral unsymmetrical cyanine dyes |
| SE519172C2 (sv) | 2000-06-07 | 2003-01-21 | Lightup Technologies Ab | Fastfas syntes av cyaninfärgämnen |
| SE519116C2 (sv) | 2001-05-10 | 2003-01-14 | Lightup Technologies Ab | Syntes och utvärdering av nya cyaninfärgämnen som binder till minor groove |
-
2001
- 2001-05-10 SE SE0101651A patent/SE519116C2/sv unknown
-
2002
- 2002-05-10 AU AU2002303054A patent/AU2002303054B2/en not_active Ceased
- 2002-05-10 CA CA002446982A patent/CA2446982C/en not_active Expired - Fee Related
- 2002-05-10 EP EP02731046A patent/EP1390433B1/en not_active Expired - Lifetime
- 2002-05-10 JP JP2002587511A patent/JP2004536900A/ja not_active Ceased
- 2002-05-10 WO PCT/SE2002/000860 patent/WO2002090443A1/en not_active Ceased
- 2002-05-10 DE DE60203005T patent/DE60203005T2/de not_active Expired - Fee Related
- 2002-05-10 AT AT02731046T patent/ATE289337T1/de not_active IP Right Cessation
-
2003
- 2003-11-10 US US10/605,961 patent/US7378240B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| SE519116C2 (sv) | 2003-01-14 |
| CA2446982C (en) | 2009-01-13 |
| WO2002090443A1 (en) | 2002-11-14 |
| CA2446982A1 (en) | 2002-11-14 |
| SE0101651L (sv) | 2002-11-11 |
| HK1060895A1 (en) | 2004-08-27 |
| AU2002303054B2 (en) | 2006-12-07 |
| JP2004536900A (ja) | 2004-12-09 |
| DE60203005D1 (de) | 2005-03-24 |
| SE0101651D0 (sv) | 2001-05-10 |
| US7378240B2 (en) | 2008-05-27 |
| EP1390433A1 (en) | 2004-02-25 |
| EP1390433B1 (en) | 2005-02-16 |
| ATE289337T1 (de) | 2005-03-15 |
| US20040132046A1 (en) | 2004-07-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8381 | Inventor (new situation) |
Inventor name: KUBISTA, MIKAEL, S-435 31 M?LNLYCKE, SE Inventor name: WESTMAN, GUNNAR, S-438 92 H?RRYDA, SE Inventor name: KARLSSON, JONAS, S-411 03 GOETEBORG, SE |
|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |