CA2446982C - Synthesis and evaluation of new cyanine dyes as minor groove of [poly(da-dt)]2 binders - Google Patents

Synthesis and evaluation of new cyanine dyes as minor groove of [poly(da-dt)]2 binders Download PDF

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Publication number
CA2446982C
CA2446982C CA002446982A CA2446982A CA2446982C CA 2446982 C CA2446982 C CA 2446982C CA 002446982 A CA002446982 A CA 002446982A CA 2446982 A CA2446982 A CA 2446982A CA 2446982 C CA2446982 C CA 2446982C
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CA
Canada
Prior art keywords
methyl
bebo
arh
ethyl
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002446982A
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English (en)
French (fr)
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CA2446982A1 (en
Inventor
Gunnar Westman
Jonas Karlsson
Mikael Kubista
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Individual
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LightUp Technologies AB
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Publication date
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Publication of CA2446982A1 publication Critical patent/CA2446982A1/en
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Publication of CA2446982C publication Critical patent/CA2446982C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/04—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Thermal Transfer Or Thermal Recording In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
CA002446982A 2001-05-10 2002-05-10 Synthesis and evaluation of new cyanine dyes as minor groove of [poly(da-dt)]2 binders Expired - Fee Related CA2446982C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0101651-8 2001-05-10
SE0101651A SE519116C2 (sv) 2001-05-10 2001-05-10 Syntes och utvärdering av nya cyaninfärgämnen som binder till minor groove
PCT/SE2002/000860 WO2002090443A1 (en) 2001-05-10 2002-05-10 Synthesis and evaluation of new cyanine dyes as minor groove of [poly(da-dt)]2 binders

Publications (2)

Publication Number Publication Date
CA2446982A1 CA2446982A1 (en) 2002-11-14
CA2446982C true CA2446982C (en) 2009-01-13

Family

ID=20284061

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002446982A Expired - Fee Related CA2446982C (en) 2001-05-10 2002-05-10 Synthesis and evaluation of new cyanine dyes as minor groove of [poly(da-dt)]2 binders

Country Status (9)

Country Link
US (1) US7378240B2 (https=)
EP (1) EP1390433B1 (https=)
JP (1) JP2004536900A (https=)
AT (1) ATE289337T1 (https=)
AU (1) AU2002303054B2 (https=)
CA (1) CA2446982C (https=)
DE (1) DE60203005T2 (https=)
SE (1) SE519116C2 (https=)
WO (1) WO2002090443A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE519116C2 (sv) 2001-05-10 2003-01-14 Lightup Technologies Ab Syntes och utvärdering av nya cyaninfärgämnen som binder till minor groove
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
JP5306811B2 (ja) * 2005-05-11 2013-10-02 ライフ テクノロジーズ コーポレーション ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用
US9433700B2 (en) 2009-04-27 2016-09-06 Medibeacon Inc. Tissue sealant compositions, vascular closure devices, and uses thereof
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
CN105765066B (zh) * 2013-11-29 2018-12-14 宝生物工程株式会社 用于定量腺伴随病毒的方法
CN108165045B (zh) * 2018-01-15 2021-03-16 广东工业大学 核酸染料化合物及其制备方法和应用
WO2023086970A2 (en) 2021-11-12 2023-05-19 Arna Genomics Us Incorporated Methods and compositions for analyzing nucleic acids and nucleases in a body fluid as indicators of disease

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5656449A (en) * 1995-03-06 1997-08-12 Molecular Probes, Inc. Neutral unsymmetrical cyanine dyes
SE519172C2 (sv) 2000-06-07 2003-01-21 Lightup Technologies Ab Fastfas syntes av cyaninfärgämnen
SE519116C2 (sv) 2001-05-10 2003-01-14 Lightup Technologies Ab Syntes och utvärdering av nya cyaninfärgämnen som binder till minor groove

Also Published As

Publication number Publication date
SE519116C2 (sv) 2003-01-14
WO2002090443A1 (en) 2002-11-14
CA2446982A1 (en) 2002-11-14
SE0101651L (sv) 2002-11-11
HK1060895A1 (en) 2004-08-27
DE60203005T2 (de) 2006-05-11
AU2002303054B2 (en) 2006-12-07
JP2004536900A (ja) 2004-12-09
DE60203005D1 (de) 2005-03-24
SE0101651D0 (sv) 2001-05-10
US7378240B2 (en) 2008-05-27
EP1390433A1 (en) 2004-02-25
EP1390433B1 (en) 2005-02-16
ATE289337T1 (de) 2005-03-15
US20040132046A1 (en) 2004-07-08

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