DE60129717D1 - Verfahren zur synthese von zellfreien proteinen - Google Patents

Verfahren zur synthese von zellfreien proteinen

Info

Publication number
DE60129717D1
DE60129717D1 DE60129717T DE60129717T DE60129717D1 DE 60129717 D1 DE60129717 D1 DE 60129717D1 DE 60129717 T DE60129717 T DE 60129717T DE 60129717 T DE60129717 T DE 60129717T DE 60129717 D1 DE60129717 D1 DE 60129717D1
Authority
DE
Germany
Prior art keywords
reaction
synthesis
cell
free protein
phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60129717T
Other languages
English (en)
Inventor
Yaeta Endo
Tatsuya Sawasaki
Tomio Ogasawara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CellFree Sciences Co Ltd
Original Assignee
CellFree Sciences Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CellFree Sciences Co Ltd filed Critical CellFree Sciences Co Ltd
Application granted granted Critical
Publication of DE60129717D1 publication Critical patent/DE60129717D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
DE60129717T 2000-08-29 2001-08-28 Verfahren zur synthese von zellfreien proteinen Expired - Fee Related DE60129717D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000259186 2000-08-29
PCT/JP2001/007356 WO2002024939A1 (fr) 2000-08-29 2001-08-28 Methode de synthese d'une proteine acellulaire

Publications (1)

Publication Number Publication Date
DE60129717D1 true DE60129717D1 (de) 2007-09-13

Family

ID=18747392

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60129717T Expired - Fee Related DE60129717D1 (de) 2000-08-29 2001-08-28 Verfahren zur synthese von zellfreien proteinen

Country Status (10)

Country Link
US (1) US6869774B2 (de)
EP (1) EP1316617B1 (de)
JP (1) JP3768190B2 (de)
KR (1) KR100749053B1 (de)
CN (2) CN1219888C (de)
AT (1) ATE368747T1 (de)
AU (2) AU2001280201B2 (de)
CA (1) CA2420984A1 (de)
DE (1) DE60129717D1 (de)
WO (1) WO2002024939A1 (de)

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US7981617B2 (en) 2000-08-30 2011-07-19 Cellfree Sciences Co., Ltd. Transcription template for cell-free protein synthesis and method using the same
JP2002125693A (ja) * 2000-10-19 2002-05-08 Toyobo Co Ltd 無細胞タンパク質合成用細胞抽出液組成物
JP2006129702A (ja) * 2002-12-05 2006-05-25 Zoegene Corp タンパク質製造方法
JPWO2004070047A1 (ja) * 2003-02-10 2006-05-25 株式会社セルフリーサイエンス 自動蛋白質合成方法及びそれを行うための装置
CN1777674B (zh) * 2003-04-25 2012-05-23 株式会社无细胞科学 高通量蛋白质合成系统及使该系统自动运行的合成装置
JP2006042601A (ja) * 2003-04-25 2006-02-16 Yaeta Endo ハイスループット合成システム
JP2005027516A (ja) * 2003-07-08 2005-02-03 Toyobo Co Ltd 細胞内成分を利用した連続的なエネルギー供給系による無細胞タンパク質合成方法
US20060188947A1 (en) * 2003-08-07 2006-08-24 Yaeta Endo Reagent for producing a protein chip
WO2005030954A1 (ja) * 2003-09-25 2005-04-07 Cellfree Sciences Co.,Ltd. 抗原物質の製造方法
WO2005123936A1 (ja) * 2004-06-15 2005-12-29 Zoegene Corporation ポリペプチド合成方法
DE102004032460B3 (de) * 2004-06-30 2005-08-18 Rina-Netzwerk Rna Technologien Gmbh Verfahren zur präparativen in vitro Proteinbiosynthese
US20060233789A1 (en) * 2004-09-24 2006-10-19 Cellfree Science Co., Ltd. Process for producing antigenic substance
JP4513495B2 (ja) * 2004-10-15 2010-07-28 株式会社島津製作所 昆虫細胞由来抽出液を用いた無細胞タンパク質合成方法
JP2008029204A (ja) * 2004-11-12 2008-02-14 Cellfree Sciences Co Ltd 無細胞タンパク質合成方法
KR100730875B1 (ko) * 2004-12-07 2007-06-20 가부시키가이샤 시마즈세이사쿠쇼 번역반응 촉진 ｄｎａ 단편 및 그것을 사용한 무세포계단백질 합성방법
DE102005013608A1 (de) * 2005-03-24 2006-09-28 Westfälische Wilhelms-Universität Münster Vorrichtung und Verfahren für die zellfreie analytische und präparative Proteinsynthese
JP2007199047A (ja) 2005-12-28 2007-08-09 Cellfree Sciences Co Ltd ビオチン化タンパク質の調製方法及び該タンパク質を用いた検出方法
KR100966655B1 (ko) 2007-08-30 2010-06-30 (주)차바이오앤디오스텍 무세포 단백질 합성을 이용한 트랜스아미나제의 활성스크리닝 방법 및 키트
JP5868955B2 (ja) 2010-04-30 2016-02-24 プロセッタ アンチバイラル インコーポレイテッド 抗ウイルス化合物
US8834694B2 (en) * 2010-12-27 2014-09-16 Mo Bio Laboratories, Inc. Dry compositions and methods for gel electrophoresis
WO2012125983A1 (en) 2011-03-17 2012-09-20 Prosetta Antiviral Inc. Antiviral treatments
EP2699241B1 (de) 2011-04-20 2016-07-27 Prosetta Antiviral Inc. Antivirale verbindungen
US20120301904A1 (en) * 2011-04-26 2012-11-29 Prosetta Antiviral, Inc Multiprotein assemblies
WO2013080811A1 (ja) 2011-11-28 2013-06-06 国立大学法人名古屋大学 リンパ球性漏斗下垂体後葉炎のバイオマーカー及びその用途
AU2013341383B2 (en) 2012-11-12 2017-08-24 The Roger B. And Ann K. Mcnamee Trust U/T/A/D Systems and methods for communicating a live event to users using the internet
CN104884606B (zh) * 2012-11-30 2020-07-10 株式会社百奥尼 自动化制备无细胞蛋白的设备及其制备蛋白质的方法
US9528137B2 (en) 2013-03-15 2016-12-27 Northwestern University Methods for cell-free protein synthesis
WO2015190857A1 (ko) 2014-06-12 2015-12-17 충남대학교산학협력단 효소를 이용하여 ｐＨ를 조절하는 무세포 단백질 합성 방법
CN105219821A (zh) * 2015-10-30 2016-01-06 浙江理工大学 一种藻酸盐组成的微胶囊中合成胞外蛋白质的方法
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
US11814621B2 (en) 2018-06-01 2023-11-14 Northwestern University Expanding the chemical substrates for genetic code reprogramming
KR102284067B1 (ko) * 2019-07-19 2021-07-30 충남대학교 산학협력단 무세포 단백질 합성을 이용하는 단백질 발현 또는 활성 증진 보조인자의 스크리닝 방법
WO2021097349A1 (en) * 2019-11-15 2021-05-20 Machine Bio Inc. Methods and systems for generating biological molecules
CN113308580A (zh) * 2021-06-18 2021-08-27 江苏支点生物科技有限公司 高通量无细胞蛋白质合成方法

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CA2064685C (en) * 1989-07-31 1996-06-18 Sergei Jurievich Ovodov Method of preparing polypeptides in a cell-free translation system
JPH04200390A (ja) * 1990-11-30 1992-07-21 Shigeyuki Yokoyama 無細胞ポリペプチド合成系によるポリペプチドの製造方法
WO1994006928A1 (en) * 1992-09-24 1994-03-31 The Public Health Research Institute Of The City Of New York, Inc. Coupled replication-translation methods and kits for protein synthesis
JP3255784B2 (ja) * 1994-01-26 2002-02-12 弥重太 遠藤 蛋白質の合成方法
JPH1080295A (ja) * 1996-07-15 1998-03-31 Nippon Flour Mills Co Ltd 無細胞タンパク合成系によるタンパクの合成方法及び装置
DE59810647D1 (de) * 1997-07-31 2004-03-04 Roche Diagnostics Gmbh Verfahren und Vorrichtung zur Durchführung biochemischer Reaktionen
EA006162B1 (ru) 1999-05-11 2005-10-27 Селлфри Сайенсиз Ко., Лтд. Препарат для бесклеточного синтеза белка, способ синтеза белка с использованием данного препарата и устройство для его осуществления
EP1221481A4 (de) 1999-10-15 2004-12-08 Yaeta Dr Endo MATRIXMOLEKüL MIT BREITER ANWENDUNG UND HOCHEFFIZIENTES, FUNKTIONELLES MITTEL DER ZELLFREIEN SYNTHESE VON PROTEINEN DURCH VERWENDUNG DESSELBEN

Also Published As

Publication number Publication date
WO2002024939A1 (fr) 2002-03-28
CA2420984A1 (en) 2002-03-28
EP1316617A1 (de) 2003-06-04
CN101092446A (zh) 2007-12-26
JPWO2002024939A1 (ja) 2004-01-29
EP1316617A4 (de) 2004-09-29
US6869774B2 (en) 2005-03-22
KR100749053B1 (ko) 2007-08-13
KR20030031992A (ko) 2003-04-23
JP3768190B2 (ja) 2006-04-19
CN1449449A (zh) 2003-10-15
EP1316617B1 (de) 2007-08-01
ATE368747T1 (de) 2007-08-15
AU2001280201B2 (en) 2006-07-13
AU8020101A (en) 2002-04-02
CN1219888C (zh) 2005-09-21
US20030162246A1 (en) 2003-08-28

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