DE60028054T2

DE60028054T2 - Fibroblasten-wachstumsfaktor-19 (fgf-19) nukleinsäure und polypeptide und verfahren zu deren verwendung für behandlung von fettleibigkeit

Fibroblasten-wachstumsfaktor-19 (fgf-19) nukleinsäure und polypeptide und verfahren zu deren verwendung für behandlung von fettleibigkeit Download PDF

Info

Publication number: DE60028054T2
Authority: DE; Germany
Prior art keywords: fgf; polypeptide; acid sequence; amino acid; nucleic acid
Prior art date: 1999-09-08
Legal status : Expired - Lifetime

Application number

DE60028054T

Other languages

German (de)

English (en)

Other versions

DE60028054D1 (de

Inventor

A. Timothy San Francisco STEWART

Elizabeth Pacifica TOMLINSON

Current Assignee

Genentech Inc

Original Assignee

Genentech Inc

Priority date

1999-09-08

Filing date

2000-03-09

Publication date

2006-12-21

1999-09-08 Priority claimed from PCT/US1999/020594 external-priority patent/WO2000015666A2/en

1999-09-15 Priority claimed from PCT/US1999/021090 external-priority patent/WO2000015796A2/en

1999-12-20 Priority claimed from PCT/US1999/030999 external-priority patent/WO2001005836A1/en

2000-02-22 Priority claimed from PCT/US2000/004414 external-priority patent/WO2001004311A1/en

2000-03-09 Application filed by Genentech Inc filed Critical Genentech Inc

2006-06-22 Publication of DE60028054D1 publication Critical patent/DE60028054D1/de

2006-12-21 Application granted granted Critical

2006-12-21 Publication of DE60028054T2 publication Critical patent/DE60028054T2/de

2020-03-10 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

102100031734 Fibroblast growth factor 19 Human genes 0.000 title claims description 510
150000007523 nucleic acids Chemical class 0.000 title claims description 253
238000000034 method Methods 0.000 title claims description 164
102000039446 nucleic acids Human genes 0.000 title claims description 89
108020004707 nucleic acids Proteins 0.000 title claims description 89
238000011282 treatment Methods 0.000 title claims description 27
101710153349 Fibroblast growth factor 19 Proteins 0.000 title description 494
108090000765 processed proteins & peptides Proteins 0.000 title description 109
102000004196 processed proteins & peptides Human genes 0.000 title description 97
229920001184 polypeptide Polymers 0.000 title description 78
210000004027 cell Anatomy 0.000 claims description 177
125000003275 alpha amino acid group Chemical group 0.000 claims description 155
150000001413 amino acids Chemical class 0.000 claims description 61
239000012634 fragment Substances 0.000 claims description 61
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 51
230000000694 effects Effects 0.000 claims description 46
125000000539 amino acid group Chemical group 0.000 claims description 45
239000002299 complementary DNA Substances 0.000 claims description 40
208000008589 Obesity Diseases 0.000 claims description 35
238000004519 manufacturing process Methods 0.000 claims description 35
235000020824 obesity Nutrition 0.000 claims description 35
238000012216 screening Methods 0.000 claims description 29
101000846394 Homo sapiens Fibroblast growth factor 19 Proteins 0.000 claims description 25
230000001965 increasing effect Effects 0.000 claims description 25
102000016267 Leptin Human genes 0.000 claims description 23
108010092277 Leptin Proteins 0.000 claims description 23
NRYBAZVQPHGZNS-ZSOCWYAHSA-N leptin Chemical compound O=C([C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CC(C)C)CCSC)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CS)C(O)=O NRYBAZVQPHGZNS-ZSOCWYAHSA-N 0.000 claims description 23
229940039781 leptin Drugs 0.000 claims description 23
108010076504 Protein Sorting Signals Proteins 0.000 claims description 22
210000001789 adipocyte Anatomy 0.000 claims description 21
230000004071 biological effect Effects 0.000 claims description 19
239000012867 bioactive agent Substances 0.000 claims description 18
230000009467 reduction Effects 0.000 claims description 18
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 17
239000003814 drug Substances 0.000 claims description 17
239000008103 glucose Substances 0.000 claims description 17
230000008859 change Effects 0.000 claims description 16
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 16
230000002829 reductive effect Effects 0.000 claims description 16
235000021588 free fatty acids Nutrition 0.000 claims description 14
230000037396 body weight Effects 0.000 claims description 13
201000010099 disease Diseases 0.000 claims description 13
238000000338 in vitro Methods 0.000 claims description 13
150000003626 triacylglycerols Chemical class 0.000 claims description 13
150000001720 carbohydrates Chemical class 0.000 claims description 12
230000004190 glucose uptake Effects 0.000 claims description 11
230000037323 metabolic rate Effects 0.000 claims description 11
108090000144 Human Proteins Proteins 0.000 claims description 9
102000003839 Human Proteins Human genes 0.000 claims description 9
230000004060 metabolic process Effects 0.000 claims description 9
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 7
235000014633 carbohydrates Nutrition 0.000 claims description 6
230000001939 inductive effect Effects 0.000 claims description 5
150000002632 lipids Chemical class 0.000 claims description 5
229930182817 methionine Natural products 0.000 claims description 5
230000007423 decrease Effects 0.000 claims description 3
230000003647 oxidation Effects 0.000 claims description 2
238000007254 oxidation reaction Methods 0.000 claims description 2
230000006698 induction Effects 0.000 claims 1
108091028043 Nucleic acid sequence Proteins 0.000 description 172
108090000623 proteins and genes Proteins 0.000 description 164
102000004169 proteins and genes Human genes 0.000 description 112
235000018102 proteins Nutrition 0.000 description 108
108020004414 DNA Proteins 0.000 description 97
239000002773 nucleotide Substances 0.000 description 71
125000003729 nucleotide group Chemical group 0.000 description 71
235000001014 amino acid Nutrition 0.000 description 62
230000027455 binding Effects 0.000 description 60
229940024606 amino acid Drugs 0.000 description 57
238000012360 testing method Methods 0.000 description 54
241000282414 Homo sapiens Species 0.000 description 50
239000013598 vector Substances 0.000 description 47
241000699670 Mus sp. Species 0.000 description 45
230000014509 gene expression Effects 0.000 description 44
239000000523 sample Substances 0.000 description 43
241001465754 Metazoa Species 0.000 description 38
230000009261 transgenic effect Effects 0.000 description 35
108091034117 Oligonucleotide Proteins 0.000 description 34
108060003951 Immunoglobulin Proteins 0.000 description 33
102000018358 immunoglobulin Human genes 0.000 description 33
239000000203 mixture Substances 0.000 description 32
239000003795 chemical substances by application Substances 0.000 description 30
240000004808 Saccharomyces cerevisiae Species 0.000 description 28
235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 28
150000001875 compounds Chemical class 0.000 description 25
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 24
239000005557 antagonist Substances 0.000 description 24
230000000295 complement effect Effects 0.000 description 24
238000009396 hybridization Methods 0.000 description 24
102000005962 receptors Human genes 0.000 description 24
230000015572 biosynthetic process Effects 0.000 description 23
108020003175 receptors Proteins 0.000 description 23
108010021625 Immunoglobulin Fragments Proteins 0.000 description 20
102000008394 Immunoglobulin Fragments Human genes 0.000 description 20
239000000427 antigen Substances 0.000 description 20
239000002609 medium Substances 0.000 description 20
238000003752 polymerase chain reaction Methods 0.000 description 20
102000036639 antigens Human genes 0.000 description 19
108091007433 antigens Proteins 0.000 description 19
108091026890 Coding region Proteins 0.000 description 18
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 18
238000006467 substitution reaction Methods 0.000 description 18
210000001519 tissue Anatomy 0.000 description 17
241000699666 Mus <mouse, genus> Species 0.000 description 16
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 16
230000000692 anti-sense effect Effects 0.000 description 16
210000004408 hybridoma Anatomy 0.000 description 16
239000000243 solution Substances 0.000 description 16
239000000126 substance Substances 0.000 description 16
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 15
241000588724 Escherichia coli Species 0.000 description 15
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 15
238000013459 approach Methods 0.000 description 15
239000013604 expression vector Substances 0.000 description 15
239000001963 growth medium Substances 0.000 description 15
238000001727 in vivo Methods 0.000 description 15
230000003993 interaction Effects 0.000 description 15
239000002502 liposome Substances 0.000 description 15
239000013612 plasmid Substances 0.000 description 15
238000013518 transcription Methods 0.000 description 15
230000035897 transcription Effects 0.000 description 15
241000699660 Mus musculus Species 0.000 description 14
238000000746 purification Methods 0.000 description 14
238000011830 transgenic mouse model Methods 0.000 description 14
239000000556 agonist Substances 0.000 description 13
238000006243 chemical reaction Methods 0.000 description 13
108020004999 messenger RNA Proteins 0.000 description 13
238000002360 preparation method Methods 0.000 description 13
108091032973 (ribonucleotides)n+m Proteins 0.000 description 12
102000053602 DNA Human genes 0.000 description 12
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 12
230000004927 fusion Effects 0.000 description 12
235000009200 high fat diet Nutrition 0.000 description 12
239000003112 inhibitor Substances 0.000 description 12
210000004962 mammalian cell Anatomy 0.000 description 12
230000004048 modification Effects 0.000 description 12
238000012986 modification Methods 0.000 description 12
108091008146 restriction endonucleases Proteins 0.000 description 12
239000011780 sodium chloride Substances 0.000 description 12
239000007787 solid Substances 0.000 description 12
241000124008 Mammalia Species 0.000 description 11
239000000872 buffer Substances 0.000 description 11
238000004113 cell culture Methods 0.000 description 11
210000004978 chinese hamster ovary cell Anatomy 0.000 description 11
229940127089 cytotoxic agent Drugs 0.000 description 11
239000003623 enhancer Substances 0.000 description 11
235000012631 food intake Nutrition 0.000 description 11
230000037406 food intake Effects 0.000 description 11
239000003446 ligand Substances 0.000 description 11
239000000047 product Substances 0.000 description 11
238000005406 washing Methods 0.000 description 11
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
102000004190 Enzymes Human genes 0.000 description 10
108090000790 Enzymes Proteins 0.000 description 10
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 10
-1 agarose) Chemical class 0.000 description 10
238000004458 analytical method Methods 0.000 description 10
238000010367 cloning Methods 0.000 description 10
229940088598 enzyme Drugs 0.000 description 10
230000013595 glycosylation Effects 0.000 description 10
238000006206 glycosylation reaction Methods 0.000 description 10
102000040430 polynucleotide Human genes 0.000 description 10
108091033319 polynucleotide Proteins 0.000 description 10
239000002157 polynucleotide Substances 0.000 description 10
230000001225 therapeutic effect Effects 0.000 description 10
239000002253 acid Substances 0.000 description 9
239000002671 adjuvant Substances 0.000 description 9
239000003153 chemical reaction reagent Substances 0.000 description 9
238000004590 computer program Methods 0.000 description 9
239000000463 material Substances 0.000 description 9
230000036284 oxygen consumption Effects 0.000 description 9
241000894007 species Species 0.000 description 9
230000014616 translation Effects 0.000 description 9
210000002700 urine Anatomy 0.000 description 9
241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 8
108700019146 Transgenes Proteins 0.000 description 8
230000000975 bioactive effect Effects 0.000 description 8
210000000349 chromosome Anatomy 0.000 description 8
238000003776 cleavage reaction Methods 0.000 description 8
238000009472 formulation Methods 0.000 description 8
229940072221 immunoglobulins Drugs 0.000 description 8
238000001802 infusion Methods 0.000 description 8
239000003550 marker Substances 0.000 description 8
239000012528 membrane Substances 0.000 description 8
230000007017 scission Effects 0.000 description 8
150000003384 small molecules Chemical class 0.000 description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
108020005544 Antisense RNA Proteins 0.000 description 7
241000894006 Bacteria Species 0.000 description 7
108010047041 Complementarity Determining Regions Proteins 0.000 description 7
241000282412 Homo Species 0.000 description 7
206010035226 Plasma cell myeloma Diseases 0.000 description 7
239000002202 Polyethylene glycol Substances 0.000 description 7
241000700159 Rattus Species 0.000 description 7
239000000074 antisense oligonucleotide Substances 0.000 description 7
238000012230 antisense oligonucleotides Methods 0.000 description 7
238000003556 assay Methods 0.000 description 7
238000005119 centrifugation Methods 0.000 description 7
125000000151 cysteine group Chemical class N[C@@H](CS)C(=O)* 0.000 description 7
235000005911 diet Nutrition 0.000 description 7
230000037213 diet Effects 0.000 description 7
229940000406 drug candidate Drugs 0.000 description 7
238000005516 engineering process Methods 0.000 description 7
239000000284 extract Substances 0.000 description 7
230000006870 function Effects 0.000 description 7
229910052751 metal Inorganic materials 0.000 description 7
239000002184 metal Substances 0.000 description 7
201000000050 myeloid neoplasm Diseases 0.000 description 7
230000001575 pathological effect Effects 0.000 description 7
229920001223 polyethylene glycol Polymers 0.000 description 7
230000008569 process Effects 0.000 description 7
239000011347 resin Substances 0.000 description 7
229920005989 resin Polymers 0.000 description 7
230000002441 reversible effect Effects 0.000 description 7
238000010561 standard procedure Methods 0.000 description 7
238000003786 synthesis reaction Methods 0.000 description 7
238000001890 transfection Methods 0.000 description 7
238000013519 translation Methods 0.000 description 7
108020004491 Antisense DNA Proteins 0.000 description 6
102100024746 Dihydrofolate reductase Human genes 0.000 description 6
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
241001302584 Escherichia coli str. K-12 substr. W3110 Species 0.000 description 6
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 6
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 6
206010028980 Neoplasm Diseases 0.000 description 6
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
238000010521 absorption reaction Methods 0.000 description 6
150000007513 acids Chemical class 0.000 description 6
210000000577 adipose tissue Anatomy 0.000 description 6
239000002246 antineoplastic agent Substances 0.000 description 6
230000001580 bacterial effect Effects 0.000 description 6
210000004899 c-terminal region Anatomy 0.000 description 6
239000003184 complementary RNA Substances 0.000 description 6
239000003636 conditioned culture medium Substances 0.000 description 6
239000002254 cytotoxic agent Substances 0.000 description 6
231100000599 cytotoxic agent Toxicity 0.000 description 6
238000012217 deletion Methods 0.000 description 6
230000037430 deletion Effects 0.000 description 6
206010012601 diabetes mellitus Diseases 0.000 description 6
238000007877 drug screening Methods 0.000 description 6
238000004520 electroporation Methods 0.000 description 6
230000002255 enzymatic effect Effects 0.000 description 6
239000000499 gel Substances 0.000 description 6
238000001415 gene therapy Methods 0.000 description 6
238000005534 hematocrit Methods 0.000 description 6
FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 6
230000001900 immune effect Effects 0.000 description 6
229940124452 immunizing agent Drugs 0.000 description 6
230000002163 immunogen Effects 0.000 description 6
238000011534 incubation Methods 0.000 description 6
238000002372 labelling Methods 0.000 description 6
239000012160 loading buffer Substances 0.000 description 6
230000007246 mechanism Effects 0.000 description 6
239000012071 phase Substances 0.000 description 6
229920000642 polymer Polymers 0.000 description 6
230000028327 secretion Effects 0.000 description 6
239000001509 sodium citrate Substances 0.000 description 6
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 6
239000007790 solid phase Substances 0.000 description 6
229940124597 therapeutic agent Drugs 0.000 description 6
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 5
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 5
102000002260 Alkaline Phosphatase Human genes 0.000 description 5
108020004774 Alkaline Phosphatase Proteins 0.000 description 5
108020000948 Antisense Oligonucleotides Proteins 0.000 description 5
206010003445 Ascites Diseases 0.000 description 5
108020004705 Codon Proteins 0.000 description 5
241000196324 Embryophyta Species 0.000 description 5
101100390711 Escherichia coli (strain K12) fhuA gene Proteins 0.000 description 5
102000018233 Fibroblast Growth Factor Human genes 0.000 description 5
108050007372 Fibroblast Growth Factor Proteins 0.000 description 5
239000004471 Glycine Substances 0.000 description 5
241000238631 Hexapoda Species 0.000 description 5
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 5
238000001042 affinity chromatography Methods 0.000 description 5
235000004279 alanine Nutrition 0.000 description 5
239000003816 antisense DNA Substances 0.000 description 5
201000011510 cancer Diseases 0.000 description 5
239000013522 chelant Substances 0.000 description 5
239000002738 chelating agent Substances 0.000 description 5
235000018417 cysteine Nutrition 0.000 description 5
229940079593 drug Drugs 0.000 description 5
238000009510 drug design Methods 0.000 description 5
230000012010 growth Effects 0.000 description 5
102000047000 human FGF19 Human genes 0.000 description 5
230000003834 intracellular effect Effects 0.000 description 5
210000004698 lymphocyte Anatomy 0.000 description 5
239000011159 matrix material Substances 0.000 description 5
230000001404 mediated effect Effects 0.000 description 5
230000035772 mutation Effects 0.000 description 5
229910052757 nitrogen Inorganic materials 0.000 description 5
239000008188 pellet Substances 0.000 description 5
238000003259 recombinant expression Methods 0.000 description 5
238000011160 research Methods 0.000 description 5
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 5
235000000346 sugar Nutrition 0.000 description 5
239000003053 toxin Substances 0.000 description 5
231100000765 toxin Toxicity 0.000 description 5
108700012359 toxins Proteins 0.000 description 5
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
108091003079 Bovine Serum Albumin Proteins 0.000 description 4
208000024172 Cardiovascular disease Diseases 0.000 description 4
108020004635 Complementary DNA Proteins 0.000 description 4
FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 4
238000002965 ELISA Methods 0.000 description 4
241000233866 Fungi Species 0.000 description 4
102100039556 Galectin-4 Human genes 0.000 description 4
108700028146 Genetic Enhancer Elements Proteins 0.000 description 4
ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 4
101000608765 Homo sapiens Galectin-4 Proteins 0.000 description 4
108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 4
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 4
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 4
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 4
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 4
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 4
239000004472 Lysine Substances 0.000 description 4
229930195725 Mannitol Natural products 0.000 description 4
229910019142 PO4 Inorganic materials 0.000 description 4
241000283984 Rodentia Species 0.000 description 4
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
239000007983 Tris buffer Substances 0.000 description 4
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
241000700605 Viruses Species 0.000 description 4
239000003242 anti bacterial agent Substances 0.000 description 4
230000001588 bifunctional effect Effects 0.000 description 4
239000000969 carrier Substances 0.000 description 4
230000015556 catabolic process Effects 0.000 description 4
238000004587 chromatography analysis Methods 0.000 description 4
238000007796 conventional method Methods 0.000 description 4
238000004132 cross linking Methods 0.000 description 4
238000013461 design Methods 0.000 description 4
238000001514 detection method Methods 0.000 description 4
238000011161 development Methods 0.000 description 4
229960000633 dextran sulfate Drugs 0.000 description 4
210000003527 eukaryotic cell Anatomy 0.000 description 4
238000000855 fermentation Methods 0.000 description 4
230000004151 fermentation Effects 0.000 description 4
108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 4
229910052739 hydrogen Inorganic materials 0.000 description 4
230000003053 immunization Effects 0.000 description 4
239000007924 injection Substances 0.000 description 4
238000002347 injection Methods 0.000 description 4
238000003780 insertion Methods 0.000 description 4
230000037431 insertion Effects 0.000 description 4
235000018977 lysine Nutrition 0.000 description 4
239000000594 mannitol Substances 0.000 description 4
235000010355 mannitol Nutrition 0.000 description 4
229960004452 methionine Drugs 0.000 description 4
125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 4
239000003094 microcapsule Substances 0.000 description 4
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
239000010452 phosphate Substances 0.000 description 4
238000000159 protein binding assay Methods 0.000 description 4
230000004850 protein–protein interaction Effects 0.000 description 4
150000003212 purines Chemical class 0.000 description 4
239000011541 reaction mixture Substances 0.000 description 4
230000001177 retroviral effect Effects 0.000 description 4
235000004400 serine Nutrition 0.000 description 4
239000011734 sodium Substances 0.000 description 4
210000004989 spleen cell Anatomy 0.000 description 4
239000006228 supernatant Substances 0.000 description 4
238000012546 transfer Methods 0.000 description 4
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
241000701447 unidentified baculovirus Species 0.000 description 4
230000003612 virological effect Effects 0.000 description 4
239000004475 Arginine Substances 0.000 description 3
208000002109 Argyria Diseases 0.000 description 3
241000222120 Candida <Saccharomycetales> Species 0.000 description 3
108090000994 Catalytic RNA Proteins 0.000 description 3
102000053642 Catalytic RNA Human genes 0.000 description 3
108010001857 Cell Surface Receptors Proteins 0.000 description 3
229920002307 Dextran Polymers 0.000 description 3
108010010803 Gelatin Proteins 0.000 description 3
AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Polymers OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 3
UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 3
102100029098 Hypoxanthine-guanine phosphoribosyltransferase Human genes 0.000 description 3
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 3
102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 3
102000006496 Immunoglobulin Heavy Chains Human genes 0.000 description 3
108010019476 Immunoglobulin Heavy Chains Proteins 0.000 description 3
102000013463 Immunoglobulin Light Chains Human genes 0.000 description 3
108010065825 Immunoglobulin Light Chains Proteins 0.000 description 3
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 3
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 3
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 3
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 3
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 3
102000018697 Membrane Proteins Human genes 0.000 description 3
108010052285 Membrane Proteins Proteins 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
101100178822 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) htrA1 gene Proteins 0.000 description 3
101100407828 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) ptr-3 gene Proteins 0.000 description 3
206010036049 Polycystic ovaries Diseases 0.000 description 3
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 3
108020004511 Recombinant DNA Proteins 0.000 description 3
101100277437 Rhizobium meliloti (strain 1021) degP1 gene Proteins 0.000 description 3
229920002684 Sepharose Polymers 0.000 description 3
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 3
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 3
IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 description 3
230000009471 action Effects 0.000 description 3
239000004480 active ingredient Substances 0.000 description 3
125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 3
238000012870 ammonium sulfate precipitation Methods 0.000 description 3
230000003321 amplification Effects 0.000 description 3
229940088710 antibiotic agent Drugs 0.000 description 3
230000010056 antibody-dependent cellular cytotoxicity Effects 0.000 description 3
230000000890 antigenic effect Effects 0.000 description 3
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 3
235000009697 arginine Nutrition 0.000 description 3
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
230000000903 blocking effect Effects 0.000 description 3
238000004364 calculation method Methods 0.000 description 3
230000001413 cellular effect Effects 0.000 description 3
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
238000004140 cleaning Methods 0.000 description 3
230000001427 coherent effect Effects 0.000 description 3
238000004440 column chromatography Methods 0.000 description 3
230000009918 complex formation Effects 0.000 description 3
229920001577 copolymer Polymers 0.000 description 3
238000012258 culturing Methods 0.000 description 3
230000002950 deficient Effects 0.000 description 3
101150018266 degP gene Proteins 0.000 description 3
238000006731 degradation reaction Methods 0.000 description 3
229960002086 dextran Drugs 0.000 description 3
238000000502 dialysis Methods 0.000 description 3
230000029087 digestion Effects 0.000 description 3
239000000539 dimer Substances 0.000 description 3
208000035475 disorder Diseases 0.000 description 3
239000012636 effector Substances 0.000 description 3
210000001671 embryonic stem cell Anatomy 0.000 description 3
239000006167 equilibration buffer Substances 0.000 description 3
150000002148 esters Chemical class 0.000 description 3
229940126864 fibroblast growth factor Drugs 0.000 description 3
238000001914 filtration Methods 0.000 description 3
239000012530 fluid Substances 0.000 description 3
235000013305 food Nutrition 0.000 description 3
108020001507 fusion proteins Proteins 0.000 description 3
102000037865 fusion proteins Human genes 0.000 description 3
239000008273 gelatin Substances 0.000 description 3
229920000159 gelatin Polymers 0.000 description 3
235000019322 gelatine Nutrition 0.000 description 3
235000011852 gelatine desserts Nutrition 0.000 description 3
230000002068 genetic effect Effects 0.000 description 3
239000011521 glass Substances 0.000 description 3
239000003102 growth factor Substances 0.000 description 3
230000036541 health Effects 0.000 description 3
238000013537 high throughput screening Methods 0.000 description 3
229940088597 hormone Drugs 0.000 description 3
239000005556 hormone Substances 0.000 description 3
230000002209 hydrophobic effect Effects 0.000 description 3
238000002649 immunization Methods 0.000 description 3
229940127121 immunoconjugate Drugs 0.000 description 3
230000001976 improved effect Effects 0.000 description 3
208000015181 infectious disease Diseases 0.000 description 3
230000002401 inhibitory effect Effects 0.000 description 3
238000002955 isolation Methods 0.000 description 3
239000004005 microsphere Substances 0.000 description 3
238000002703 mutagenesis Methods 0.000 description 3
231100000350 mutagenesis Toxicity 0.000 description 3
230000007935 neutral effect Effects 0.000 description 3
230000003472 neutralizing effect Effects 0.000 description 3
238000003199 nucleic acid amplification method Methods 0.000 description 3
239000000546 pharmaceutical excipient Substances 0.000 description 3
229920002401 polyacrylamide Polymers 0.000 description 3
201000010065 polycystic ovary syndrome Diseases 0.000 description 3
239000001267 polyvinylpyrrolidone Substances 0.000 description 3
229920000036 polyvinylpyrrolidone Polymers 0.000 description 3
235000013855 polyvinylpyrrolidone Nutrition 0.000 description 3
239000002244 precipitate Substances 0.000 description 3
238000001556 precipitation Methods 0.000 description 3
239000002243 precursor Substances 0.000 description 3
230000002062 proliferating effect Effects 0.000 description 3
238000001742 protein purification Methods 0.000 description 3
230000002285 radioactive effect Effects 0.000 description 3
231100000272 reduced body weight Toxicity 0.000 description 3
108091092562 ribozyme Proteins 0.000 description 3
239000012679 serum free medium Substances 0.000 description 3
229910052708 sodium Inorganic materials 0.000 description 3
239000001488 sodium phosphate Substances 0.000 description 3
229910000162 sodium phosphate Inorganic materials 0.000 description 3
230000006641 stabilisation Effects 0.000 description 3
238000011105 stabilization Methods 0.000 description 3
239000003381 stabilizer Substances 0.000 description 3
238000013268 sustained release Methods 0.000 description 3
239000012730 sustained-release form Substances 0.000 description 3
238000002560 therapeutic procedure Methods 0.000 description 3
125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 3
230000005030 transcription termination Effects 0.000 description 3
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 3
238000010396 two-hybrid screening Methods 0.000 description 3
208000001072 type 2 diabetes mellitus Diseases 0.000 description 3
241000701161 unidentified adenovirus Species 0.000 description 3
241001430294 unidentified retrovirus Species 0.000 description 3
239000003981 vehicle Substances 0.000 description 3
238000002424 x-ray crystallography Methods 0.000 description 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 2
VPFUWHKTPYPNGT-UHFFFAOYSA-N 3-(3,4-dihydroxyphenyl)-1-(5-hydroxy-2,2-dimethylchromen-6-yl)propan-1-one Chemical compound OC1=C2C=CC(C)(C)OC2=CC=C1C(=O)CCC1=CC=C(O)C(O)=C1 VPFUWHKTPYPNGT-UHFFFAOYSA-N 0.000 description 2
OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 2
QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 2
TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 2
GANZODCWZFAEGN-UHFFFAOYSA-N 5-mercapto-2-nitro-benzoic acid Chemical compound OC(=O)C1=CC(S)=CC=C1[N+]([O-])=O GANZODCWZFAEGN-UHFFFAOYSA-N 0.000 description 2
102000013563 Acid Phosphatase Human genes 0.000 description 2
108010051457 Acid Phosphatase Proteins 0.000 description 2
229920000936 Agarose Polymers 0.000 description 2
108010088751 Albumins Proteins 0.000 description 2
102000009027 Albumins Human genes 0.000 description 2
102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 description 2
101710193111 All-trans-retinol dehydrogenase [NAD(+)] ADH4 Proteins 0.000 description 2
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 2
241000972773 Aulopiformes Species 0.000 description 2
108090001008 Avidin Proteins 0.000 description 2
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
241000283690 Bos taurus Species 0.000 description 2
206010006187 Breast cancer Diseases 0.000 description 2
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
102000000844 Cell Surface Receptors Human genes 0.000 description 2
241000282693 Cercopithecidae Species 0.000 description 2
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
108091035707 Consensus sequence Proteins 0.000 description 2
241000699802 Cricetulus griseus Species 0.000 description 2
239000004971 Cross linker Substances 0.000 description 2
108090000695 Cytokines Proteins 0.000 description 2
102000004127 Cytokines Human genes 0.000 description 2
241000701022 Cytomegalovirus Species 0.000 description 2
FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 2
230000004544 DNA amplification Effects 0.000 description 2
238000001712 DNA sequencing Methods 0.000 description 2
230000004568 DNA-binding Effects 0.000 description 2
201000004624 Dermatitis Diseases 0.000 description 2
239000004375 Dextrin Substances 0.000 description 2
229920001353 Dextrin Polymers 0.000 description 2
AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 2
239000006144 Dulbecco’s modiﬁed Eagle's medium Substances 0.000 description 2
208000005189 Embolism Diseases 0.000 description 2
241000588921 Enterobacteriaceae Species 0.000 description 2
108010013369 Enteropeptidase Proteins 0.000 description 2
102100029727 Enteropeptidase Human genes 0.000 description 2
108091008794 FGF receptors Proteins 0.000 description 2
102000044168 Fibroblast Growth Factor Receptor Human genes 0.000 description 2
102000005731 Glucose-6-phosphate isomerase Human genes 0.000 description 2
108010070600 Glucose-6-phosphate isomerase Proteins 0.000 description 2
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
108010031186 Glycoside Hydrolases Proteins 0.000 description 2
102000005744 Glycoside Hydrolases Human genes 0.000 description 2
101000878605 Homo sapiens Low affinity immunoglobulin epsilon Fc receptor Proteins 0.000 description 2
208000035150 Hypercholesterolemia Diseases 0.000 description 2
206010020772 Hypertension Diseases 0.000 description 2
206010022489 Insulin Resistance Diseases 0.000 description 2
241000235649 Kluyveromyces Species 0.000 description 2
244000285963 Kluyveromyces fragilis Species 0.000 description 2
241001138401 Kluyveromyces lactis Species 0.000 description 2
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
LRQKBLKVPFOOQJ-YFKPBYRVSA-N L-norleucine Chemical compound CCCC[C@H]([NH3+])C([O-])=O LRQKBLKVPFOOQJ-YFKPBYRVSA-N 0.000 description 2
JVTAAEKCZFNVCJ-UHFFFAOYSA-N Lactic Acid Natural products CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
102100038007 Low affinity immunoglobulin epsilon Fc receptor Human genes 0.000 description 2
102100029185 Low affinity immunoglobulin gamma Fc region receptor III-B Human genes 0.000 description 2
241001529936 Murinae Species 0.000 description 2
CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 2
108020005187 Oligonucleotide Probes Proteins 0.000 description 2
108010038807 Oligopeptides Proteins 0.000 description 2
102000015636 Oligopeptides Human genes 0.000 description 2
108700026244 Open Reading Frames Proteins 0.000 description 2
241000283973 Oryctolagus cuniculus Species 0.000 description 2
238000012408 PCR amplification Methods 0.000 description 2
108091005804 Peptidases Proteins 0.000 description 2
102000035195 Peptidases Human genes 0.000 description 2
102000007079 Peptide Fragments Human genes 0.000 description 2
108010033276 Peptide Fragments Proteins 0.000 description 2
241000235648 Pichia Species 0.000 description 2
239000004365 Protease Substances 0.000 description 2
101100084022 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) lapA gene Proteins 0.000 description 2
108020005091 Replication Origin Proteins 0.000 description 2
108010039491 Ricin Proteins 0.000 description 2
241000235070 Saccharomyces Species 0.000 description 2
229920005654 Sephadex Polymers 0.000 description 2
239000012507 Sephadex™ Substances 0.000 description 2
241000607720 Serratia Species 0.000 description 2
108010071390 Serum Albumin Proteins 0.000 description 2
102000007562 Serum Albumin Human genes 0.000 description 2
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
101150006914 TRP1 gene Proteins 0.000 description 2
239000004098 Tetracycline Substances 0.000 description 2
208000001435 Thromboembolism Diseases 0.000 description 2
102000002262 Thromboplastin Human genes 0.000 description 2
108010000499 Thromboplastin Proteins 0.000 description 2
101710120037 Toxin CcdB Proteins 0.000 description 2
ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Chemical compound CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
206010046996 Varicose vein Diseases 0.000 description 2
241000251539 Vertebrata <Metazoa> Species 0.000 description 2
108020005202 Viral DNA Proteins 0.000 description 2
208000027418 Wounds and injury Diseases 0.000 description 2
201000010272 acanthosis nigricans Diseases 0.000 description 2
230000004913 activation Effects 0.000 description 2
238000001261 affinity purification Methods 0.000 description 2
230000002776 aggregation Effects 0.000 description 2
238000004220 aggregation Methods 0.000 description 2
230000009435 amidation Effects 0.000 description 2
238000007112 amidation reaction Methods 0.000 description 2
150000001412 amines Chemical group 0.000 description 2
229960003896 aminopterin Drugs 0.000 description 2
229960000723 ampicillin Drugs 0.000 description 2
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
239000003963 antioxidant agent Substances 0.000 description 2
235000006708 antioxidants Nutrition 0.000 description 2
125000003118 aryl group Chemical group 0.000 description 2
229960005070 ascorbic acid Drugs 0.000 description 2
235000010323 ascorbic acid Nutrition 0.000 description 2
239000011668 ascorbic acid Substances 0.000 description 2
235000009582 asparagine Nutrition 0.000 description 2
229960001230 asparagine Drugs 0.000 description 2
208000010668 atopic eczema Diseases 0.000 description 2
244000052616 bacterial pathogen Species 0.000 description 2
230000008901 benefit Effects 0.000 description 2
108010051210 beta-Fructofuranosidase Proteins 0.000 description 2
102000005936 beta-Galactosidase Human genes 0.000 description 2
108010005774 beta-Galactosidase Proteins 0.000 description 2
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
230000003115 biocidal effect Effects 0.000 description 2
230000033228 biological regulation Effects 0.000 description 2
210000000988 bone and bone Anatomy 0.000 description 2
229940098773 bovine serum albumin Drugs 0.000 description 2
DQXBYHZEEUGOBF-UHFFFAOYSA-N but-3-enoic acid;ethene Chemical compound C=C.OC(=O)CC=C DQXBYHZEEUGOBF-UHFFFAOYSA-N 0.000 description 2
239000001506 calcium phosphate Substances 0.000 description 2
229910000389 calcium phosphate Inorganic materials 0.000 description 2
235000011010 calcium phosphates Nutrition 0.000 description 2
235000019577 caloric intake Nutrition 0.000 description 2
239000004202 carbamide Substances 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
210000000170 cell membrane Anatomy 0.000 description 2
201000001883 cholelithiasis Diseases 0.000 description 2
HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
239000012539 chromatography resin Substances 0.000 description 2
230000002759 chromosomal effect Effects 0.000 description 2
238000012875 competitive assay Methods 0.000 description 2
230000009137 competitive binding Effects 0.000 description 2
238000005094 computer simulation Methods 0.000 description 2
230000021615 conjugation Effects 0.000 description 2
230000001351 cycling effect Effects 0.000 description 2
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
230000006378 damage Effects 0.000 description 2
239000003405 delayed action preparation Substances 0.000 description 2
239000003599 detergent Substances 0.000 description 2
235000019425 dextrin Nutrition 0.000 description 2
238000002405 diagnostic procedure Methods 0.000 description 2
238000010586 diagram Methods 0.000 description 2
SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 2
150000002016 disaccharides Chemical class 0.000 description 2
125000002228 disulfide group Chemical group 0.000 description 2
150000004662 dithiols Chemical class 0.000 description 2
239000003937 drug carrier Substances 0.000 description 2
238000012377 drug delivery Methods 0.000 description 2
230000002526 effect on cardiovascular system Effects 0.000 description 2
CTSPAMFJBXKSOY-UHFFFAOYSA-N ellipticine Chemical compound N1=CC=C2C(C)=C(NC=3C4=CC=CC=3)C4=C(C)C2=C1 CTSPAMFJBXKSOY-UHFFFAOYSA-N 0.000 description 2
239000000839 emulsion Substances 0.000 description 2
201000010063 epididymitis Diseases 0.000 description 2
239000005038 ethylene vinyl acetate Substances 0.000 description 2
238000005194 fractionation Methods 0.000 description 2
238000004108 freeze drying Methods 0.000 description 2
239000012737 fresh medium Substances 0.000 description 2
125000000524 functional group Chemical group 0.000 description 2
230000002538 fungal effect Effects 0.000 description 2
238000001502 gel electrophoresis Methods 0.000 description 2
238000002523 gelfiltration Methods 0.000 description 2
210000004602 germ cell Anatomy 0.000 description 2
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
235000004554 glutamine Nutrition 0.000 description 2
150000004676 glycans Chemical class 0.000 description 2
102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
229940093915 gynecological organic acid Drugs 0.000 description 2
108010037896 heparin-binding hemagglutinin Proteins 0.000 description 2
238000002744 homologous recombination Methods 0.000 description 2
230000006801 homologous recombination Effects 0.000 description 2
239000000017 hydrogel Substances 0.000 description 2
229920001477 hydrophilic polymer Polymers 0.000 description 2
125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
150000002463 imidates Chemical class 0.000 description 2
238000003018 immunoassay Methods 0.000 description 2
230000016784 immunoglobulin production Effects 0.000 description 2
238000011532 immunohistochemical staining Methods 0.000 description 2
238000001114 immunoprecipitation Methods 0.000 description 2
230000002637 immunotoxin Effects 0.000 description 2
239000002596 immunotoxin Substances 0.000 description 2
229940051026 immunotoxin Drugs 0.000 description 2
231100000608 immunotoxin Toxicity 0.000 description 2
230000008676 import Effects 0.000 description 2
238000007901 in situ hybridization Methods 0.000 description 2
238000011065 in-situ storage Methods 0.000 description 2
230000005764 inhibitory process Effects 0.000 description 2
208000014674 injury Diseases 0.000 description 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
238000001990 intravenous administration Methods 0.000 description 2
239000001573 invertase Substances 0.000 description 2
235000011073 invertase Nutrition 0.000 description 2
DRAVOWXCEBXPTN-UHFFFAOYSA-N isoguanine Chemical compound NC1=NC(=O)NC2=C1NC=N2 DRAVOWXCEBXPTN-UHFFFAOYSA-N 0.000 description 2
210000003734 kidney Anatomy 0.000 description 2
210000000265 leukocyte Anatomy 0.000 description 2
RGLRXNKKBLIBQS-XNHQSDQCSA-N leuprolide acetate Chemical compound CC(O)=O.CCNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)CC1=CC=C(O)C=C1 RGLRXNKKBLIBQS-XNHQSDQCSA-N 0.000 description 2
210000005229 liver cell Anatomy 0.000 description 2
238000011068 loading method Methods 0.000 description 2
238000012423 maintenance Methods 0.000 description 2
238000013507 mapping Methods 0.000 description 2
238000005259 measurement Methods 0.000 description 2
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 2
244000005700 microbiome Species 0.000 description 2
238000000520 microinjection Methods 0.000 description 2
238000002156 mixing Methods 0.000 description 2
238000010369 molecular cloning Methods 0.000 description 2
150000002772 monosaccharides Chemical class 0.000 description 2
210000003205 muscle Anatomy 0.000 description 2
229930014626 natural product Natural products 0.000 description 2
239000002736 nonionic surfactant Substances 0.000 description 2
231100000252 nontoxic Toxicity 0.000 description 2
230000003000 nontoxic effect Effects 0.000 description 2
235000015097 nutrients Nutrition 0.000 description 2
230000035764 nutrition Effects 0.000 description 2
235000016709 nutrition Nutrition 0.000 description 2
239000002751 oligonucleotide probe Substances 0.000 description 2
101150093139 ompT gene Proteins 0.000 description 2
210000000056 organ Anatomy 0.000 description 2
150000007524 organic acids Chemical class 0.000 description 2
235000005985 organic acids Nutrition 0.000 description 2
150000002894 organic compounds Chemical class 0.000 description 2
230000000399 orthopedic effect Effects 0.000 description 2
201000008482 osteoarthritis Diseases 0.000 description 2
210000001672 ovary Anatomy 0.000 description 2
230000002018 overexpression Effects 0.000 description 2
239000002245 particle Substances 0.000 description 2
101150101567 pat-2 gene Proteins 0.000 description 2
238000010647 peptide synthesis reaction Methods 0.000 description 2
239000008194 pharmaceutical composition Substances 0.000 description 2
239000002831 pharmacologic agent Substances 0.000 description 2
101150009573 phoA gene Proteins 0.000 description 2
150000004713 phosphodiesters Chemical group 0.000 description 2
230000026731 phosphorylation Effects 0.000 description 2
238000006366 phosphorylation reaction Methods 0.000 description 2
229920001983 poloxamer Polymers 0.000 description 2
229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 description 2
229920000747 poly(lactic acid) Polymers 0.000 description 2
229920001282 polysaccharide Polymers 0.000 description 2
239000005017 polysaccharide Substances 0.000 description 2
229920000136 polysorbate Polymers 0.000 description 2
229920002451 polyvinyl alcohol Polymers 0.000 description 2
239000011148 porous material Substances 0.000 description 2
238000012545 processing Methods 0.000 description 2
239000013587 production medium Substances 0.000 description 2
150000003230 pyrimidines Chemical class 0.000 description 2
238000003127 radioimmunoassay Methods 0.000 description 2
230000006798 recombination Effects 0.000 description 2
238000005215 recombination Methods 0.000 description 2
238000011084 recovery Methods 0.000 description 2
230000010076 replication Effects 0.000 description 2
235000019515 salmon Nutrition 0.000 description 2
230000003248 secreting effect Effects 0.000 description 2
239000006152 selective media Substances 0.000 description 2
238000002864 sequence alignment Methods 0.000 description 2
238000012163 sequencing technique Methods 0.000 description 2
210000002966 serum Anatomy 0.000 description 2
FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 2
229940048086 sodium pyrophosphate Drugs 0.000 description 2
GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
239000002904 solvent Substances 0.000 description 2
238000000527 sonication Methods 0.000 description 2
239000000600 sorbitol Substances 0.000 description 2
230000010473 stable expression Effects 0.000 description 2
238000003860 storage Methods 0.000 description 2
239000007929 subcutaneous injection Substances 0.000 description 2
239000000758 substrate Substances 0.000 description 2
150000005846 sugar alcohols Chemical class 0.000 description 2
150000008163 sugars Chemical class 0.000 description 2
239000004094 surface-active agent Substances 0.000 description 2
238000001308 synthesis method Methods 0.000 description 2
238000010189 synthetic method Methods 0.000 description 2
230000008685 targeting Effects 0.000 description 2
229960002180 tetracycline Drugs 0.000 description 2
229930101283 tetracycline Natural products 0.000 description 2
235000019364 tetracycline Nutrition 0.000 description 2
150000003522 tetracyclines Chemical class 0.000 description 2
235000019818 tetrasodium diphosphate Nutrition 0.000 description 2
239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 2
125000003396 thiol group Chemical group [H]S* 0.000 description 2
235000008521 threonine Nutrition 0.000 description 2
229940104230 thymidine Drugs 0.000 description 2
RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
239000003104 tissue culture media Substances 0.000 description 2
231100000607 toxicokinetics Toxicity 0.000 description 2
230000009466 transformation Effects 0.000 description 2
QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
235000002374 tyrosine Nutrition 0.000 description 2
229960005486 vaccine Drugs 0.000 description 2
208000027185 varicose disease Diseases 0.000 description 2
239000011534 wash buffer Substances 0.000 description 2
238000001262 western blot Methods 0.000 description 2
210000005253 yeast cell Anatomy 0.000 description 2
FXYPGCIGRDZWNR-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-[[3-(2,5-dioxopyrrolidin-1-yl)oxy-3-oxopropyl]disulfanyl]propanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCSSCCC(=O)ON1C(=O)CCC1=O FXYPGCIGRDZWNR-UHFFFAOYSA-N 0.000 description 1
DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
XMQUEQJCYRFIQS-YFKPBYRVSA-N (2s)-2-amino-5-ethoxy-5-oxopentanoic acid Chemical compound CCOC(=O)CC[C@H](N)C(O)=O XMQUEQJCYRFIQS-YFKPBYRVSA-N 0.000 description 1
IEUUDEWWMRQUDS-UHFFFAOYSA-N (6-azaniumylidene-1,6-dimethoxyhexylidene)azanium;dichloride Chemical compound Cl.Cl.COC(=N)CCCCC(=N)OC IEUUDEWWMRQUDS-UHFFFAOYSA-N 0.000 description 1
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
RKDVKSZUMVYZHH-UHFFFAOYSA-N 1,4-dioxane-2,5-dione Chemical compound O=C1COC(=O)CO1 RKDVKSZUMVYZHH-UHFFFAOYSA-N 0.000 description 1
VILFTWLXLYIEMV-UHFFFAOYSA-N 1,5-difluoro-2,4-dinitrobenzene Chemical compound [O-][N+](=O)C1=CC([N+]([O-])=O)=C(F)C=C1F VILFTWLXLYIEMV-UHFFFAOYSA-N 0.000 description 1
OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
125000003287 1H-imidazol-4-ylmethyl group Chemical group [H]N1C([H])=NC(C([H])([H])[*])=C1[H] 0.000 description 1
VILCJCGEZXAXTO-UHFFFAOYSA-N 2,2,2-tetramine Chemical compound NCCNCCNCCN VILCJCGEZXAXTO-UHFFFAOYSA-N 0.000 description 1
YBBNVCVOACOHIG-UHFFFAOYSA-N 2,2-diamino-1,4-bis(4-azidophenyl)-3-butylbutane-1,4-dione Chemical compound C=1C=C(N=[N+]=[N-])C=CC=1C(=O)C(N)(N)C(CCCC)C(=O)C1=CC=C(N=[N+]=[N-])C=C1 YBBNVCVOACOHIG-UHFFFAOYSA-N 0.000 description 1
150000003923 2,5-pyrrolediones Chemical class 0.000 description 1
FZDFGHZZPBUTGP-UHFFFAOYSA-N 2-[[2-[bis(carboxymethyl)amino]-3-(4-isothiocyanatophenyl)propyl]-[2-[bis(carboxymethyl)amino]propyl]amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)C(C)CN(CC(O)=O)CC(N(CC(O)=O)CC(O)=O)CC1=CC=C(N=C=S)C=C1 FZDFGHZZPBUTGP-UHFFFAOYSA-N 0.000 description 1
WYMDDFRYORANCC-UHFFFAOYSA-N 2-[[3-[bis(carboxymethyl)amino]-2-hydroxypropyl]-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)CN(CC(O)=O)CC(O)=O WYMDDFRYORANCC-UHFFFAOYSA-N 0.000 description 1
FBUTXZSKZCQABC-UHFFFAOYSA-N 2-amino-1-methyl-7h-purine-6-thione Chemical compound S=C1N(C)C(N)=NC2=C1NC=N2 FBUTXZSKZCQABC-UHFFFAOYSA-N 0.000 description 1
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
XQCZBXHVTFVIFE-UHFFFAOYSA-N 2-amino-4-hydroxypyrimidine Chemical compound NC1=NC=CC(O)=N1 XQCZBXHVTFVIFE-UHFFFAOYSA-N 0.000 description 1
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
XBBVURRQGJPTHH-UHFFFAOYSA-N 2-hydroxyacetic acid;2-hydroxypropanoic acid Chemical compound OCC(O)=O.CC(O)C(O)=O XBBVURRQGJPTHH-UHFFFAOYSA-N 0.000 description 1
BIGBDMFRWJRLGJ-UHFFFAOYSA-N 3-benzyl-1,5-didiazoniopenta-1,4-diene-2,4-diolate Chemical compound [N-]=[N+]=CC(=O)C(C(=O)C=[N+]=[N-])CC1=CC=CC=C1 BIGBDMFRWJRLGJ-UHFFFAOYSA-N 0.000 description 1
PQYGLZAKNWQTCV-HNNXBMFYSA-N 4-[N'-(2-hydroxyethyl)thioureido]-L-benzyl EDTA Chemical compound OCCNC(=S)NC1=CC=C(C[C@@H](CN(CC(O)=O)CC(O)=O)N(CC(O)=O)CC(O)=O)C=C1 PQYGLZAKNWQTCV-HNNXBMFYSA-N 0.000 description 1
NLPWSMKACWGINL-UHFFFAOYSA-N 4-azido-2-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(N=[N+]=[N-])C=C1O NLPWSMKACWGINL-UHFFFAOYSA-N 0.000 description 1
108010066676 Abrin Proteins 0.000 description 1
102000007469 Actins Human genes 0.000 description 1
108010085238 Actins Proteins 0.000 description 1
208000024893 Acute lymphoblastic leukemia Diseases 0.000 description 1
208000014697 Acute lymphocytic leukaemia Diseases 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
241000589155 Agrobacterium tumefaciens Species 0.000 description 1
101710187573 Alcohol dehydrogenase 2 Proteins 0.000 description 1
101710133776 Alcohol dehydrogenase class-3 Proteins 0.000 description 1
102100023635 Alpha-fetoprotein Human genes 0.000 description 1
206010003210 Arteriosclerosis Diseases 0.000 description 1
241000228212 Aspergillus Species 0.000 description 1
241000351920 Aspergillus nidulans Species 0.000 description 1
241000228245 Aspergillus niger Species 0.000 description 1
101000669426 Aspergillus restrictus Ribonuclease mitogillin Proteins 0.000 description 1
102100022717 Atypical chemokine receptor 1 Human genes 0.000 description 1
241000713842 Avian sarcoma virus Species 0.000 description 1
241000194108 Bacillus licheniformis Species 0.000 description 1
235000014469 Bacillus subtilis Nutrition 0.000 description 1
101001011741 Bos taurus Insulin Proteins 0.000 description 1
101000766308 Bos taurus Serotransferrin Proteins 0.000 description 1
241000701822 Bovine papillomavirus Species 0.000 description 1
208000026310 Breast neoplasm Diseases 0.000 description 1
208000032841 Bulimia Diseases 0.000 description 1
206010006550 Bulimia nervosa Diseases 0.000 description 1
YFSNBQBDMHKKQP-UHFFFAOYSA-N C(C)(=O)O.C(C)(=O)O.C(C)(=O)O.C(C)(=O)O.C(C)(=O)O.N(=C=S)C1(CNCC(NCCN)C)CC=CC=C1 Chemical compound C(C)(=O)O.C(C)(=O)O.C(C)(=O)O.C(C)(=O)O.C(C)(=O)O.N(=C=S)C1(CNCC(NCCN)C)CC=CC=C1 YFSNBQBDMHKKQP-UHFFFAOYSA-N 0.000 description 1
125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
241000222122 Candida albicans Species 0.000 description 1
241000282472 Canis lupus familiaris Species 0.000 description 1
101710158575 Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase Proteins 0.000 description 1
241000283707 Capra Species 0.000 description 1
108090000565 Capsid Proteins Proteins 0.000 description 1
102100023321 Ceruloplasmin Human genes 0.000 description 1
208000017667 Chronic Disease Diseases 0.000 description 1
108091062157 Cis-regulatory element Proteins 0.000 description 1
238000011537 Coomassie blue staining Methods 0.000 description 1
208000009798 Craniopharyngioma Diseases 0.000 description 1
241000699800 Cricetinae Species 0.000 description 1
108700032819 Croton tiglium crotin II Proteins 0.000 description 1
IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
QNAYBMKLOCPYGJ-UWTATZPHSA-N D-alanine Chemical compound C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 1
QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 1
CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
108090000204 Dipeptidase 1 Proteins 0.000 description 1
102000016607 Diphtheria Toxin Human genes 0.000 description 1
108010053187 Diphtheria Toxin Proteins 0.000 description 1
241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
206010013883 Dwarfism Diseases 0.000 description 1
101710202200 Endolysin A Proteins 0.000 description 1
241000588914 Enterobacter Species 0.000 description 1
101710146739 Enterotoxin Proteins 0.000 description 1
241000283086 Equidae Species 0.000 description 1
241000588698 Erwinia Species 0.000 description 1
241000588722 Escherichia Species 0.000 description 1
101000867232 Escherichia coli Heat-stable enterotoxin II Proteins 0.000 description 1
241001646716 Escherichia coli K-12 Species 0.000 description 1
PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
241000206602 Eukaryota Species 0.000 description 1
101710082714 Exotoxin A Proteins 0.000 description 1
108010087819 Fc receptors Proteins 0.000 description 1
102000009109 Fc receptors Human genes 0.000 description 1
241000282326 Felis catus Species 0.000 description 1
229920001917 Ficoll Polymers 0.000 description 1
BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
241000700662 Fowlpox virus Species 0.000 description 1
201000001498 Froelich syndrome Diseases 0.000 description 1
241000226288 Gekko rossi Species 0.000 description 1
108700004714 Gelonium multiflorum GEL Proteins 0.000 description 1
108700039691 Genetic Promoter Regions Proteins 0.000 description 1
108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
102100022624 Glucoamylase Human genes 0.000 description 1
102000030595 Glucokinase Human genes 0.000 description 1
108010021582 Glucokinase Proteins 0.000 description 1
208000002705 Glucose Intolerance Diseases 0.000 description 1
206010018429 Glucose tolerance impaired Diseases 0.000 description 1
JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 1
244000068988 Glycine max Species 0.000 description 1
235000010469 Glycine max Nutrition 0.000 description 1
108010051696 Growth Hormone Proteins 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
208000031886 HIV Infections Diseases 0.000 description 1
208000037357 HIV infectious disease Diseases 0.000 description 1
101100082540 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) pcp gene Proteins 0.000 description 1
241001149669 Hanseniaspora Species 0.000 description 1
241000700721 Hepatitis B virus Species 0.000 description 1
229920000209 Hexadimethrine bromide Polymers 0.000 description 1
102000005548 Hexokinase Human genes 0.000 description 1
108700040460 Hexokinases Proteins 0.000 description 1
102100026122 High affinity immunoglobulin gamma Fc receptor I Human genes 0.000 description 1
LYCVKHSJGDMDLM-LURJTMIESA-N His-Gly Chemical compound OC(=O)CNC(=O)[C@@H](N)CC1=CN=CN1 LYCVKHSJGDMDLM-LURJTMIESA-N 0.000 description 1
101000678879 Homo sapiens Atypical chemokine receptor 1 Proteins 0.000 description 1
101000913074 Homo sapiens High affinity immunoglobulin gamma Fc receptor I Proteins 0.000 description 1
101000917826 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor II-a Proteins 0.000 description 1
101000917824 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor II-b Proteins 0.000 description 1
101000917858 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-A Proteins 0.000 description 1
101000917839 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-B Proteins 0.000 description 1
101000914514 Homo sapiens T-cell-specific surface glycoprotein CD28 Proteins 0.000 description 1
108010001336 Horseradish Peroxidase Proteins 0.000 description 1
102000002265 Human Growth Hormone Human genes 0.000 description 1
108010000521 Human Growth Hormone Proteins 0.000 description 1
239000000854 Human Growth Hormone Substances 0.000 description 1
241000701109 Human adenovirus 2 Species 0.000 description 1
241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
206010020710 Hyperphagia Diseases 0.000 description 1
108010073807 IgG Receptors Proteins 0.000 description 1
108010058683 Immobilized Proteins Proteins 0.000 description 1
102000009786 Immunoglobulin Constant Regions Human genes 0.000 description 1
108010009817 Immunoglobulin Constant Regions Proteins 0.000 description 1
108700005091 Immunoglobulin Genes Proteins 0.000 description 1
102000012745 Immunoglobulin Subunits Human genes 0.000 description 1
108010079585 Immunoglobulin Subunits Proteins 0.000 description 1
108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
102000017727 Immunoglobulin Variable Region Human genes 0.000 description 1
208000026350 Inborn Genetic disease Diseases 0.000 description 1
229930010555 Inosine Natural products 0.000 description 1
UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
108090001061 Insulin Proteins 0.000 description 1
102000004877 Insulin Human genes 0.000 description 1
238000012695 Interfacial polymerization Methods 0.000 description 1
102000014150 Interferons Human genes 0.000 description 1
108010050904 Interferons Proteins 0.000 description 1
102000000588 Interleukin-2 Human genes 0.000 description 1
108010002350 Interleukin-2 Proteins 0.000 description 1
108091092195 Intron Proteins 0.000 description 1
241000588748 Klebsiella Species 0.000 description 1
241000235058 Komagataella pastoris Species 0.000 description 1
RHGKLRLOHDJJDR-BYPYZUCNSA-N L-citrulline Chemical compound NC(=O)NCCC[C@H]([NH3+])C([O-])=O RHGKLRLOHDJJDR-BYPYZUCNSA-N 0.000 description 1
JTTHKOPSMAVJFE-VIFPVBQESA-N L-homophenylalanine Chemical compound OC(=O)[C@@H](N)CCC1=CC=CC=C1 JTTHKOPSMAVJFE-VIFPVBQESA-N 0.000 description 1
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
241000481961 Lachancea thermotolerans Species 0.000 description 1
241000235651 Lachancea waltii Species 0.000 description 1
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
108010000817 Leuprolide Proteins 0.000 description 1
102000004882 Lipase Human genes 0.000 description 1
108090001060 Lipase Proteins 0.000 description 1
239000004367 Lipase Substances 0.000 description 1
102100029204 Low affinity immunoglobulin gamma Fc region receptor II-a Human genes 0.000 description 1
DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
239000004907 Macro-emulsion Substances 0.000 description 1
GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
102000003792 Metallothionein Human genes 0.000 description 1
108090000157 Metallothionein Proteins 0.000 description 1
244000302512 Momordica charantia Species 0.000 description 1
235000009811 Momordica charantia Nutrition 0.000 description 1
101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
102000016349 Myosin Light Chains Human genes 0.000 description 1
108010067385 Myosin Light Chains Proteins 0.000 description 1
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
241001460678 Napo <wasp> Species 0.000 description 1
RHGKLRLOHDJJDR-UHFFFAOYSA-N Ndelta-carbamoyl-DL-ornithine Natural products OC(=O)C(N)CCCNC(N)=O RHGKLRLOHDJJDR-UHFFFAOYSA-N 0.000 description 1
229930193140 Neomycin Natural products 0.000 description 1
241000221960 Neurospora Species 0.000 description 1
241000221961 Neurospora crassa Species 0.000 description 1
238000000636 Northern blotting Methods 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
108091007494 Nucleic acid- binding domains Proteins 0.000 description 1
108010067372 Pancreatic elastase Proteins 0.000 description 1
102000016387 Pancreatic elastase Human genes 0.000 description 1
108090000526 Papain Proteins 0.000 description 1
241001494479 Pecora Species 0.000 description 1
108010087702 Penicillinase Proteins 0.000 description 1
241000228143 Penicillium Species 0.000 description 1
102000057297 Pepsin A Human genes 0.000 description 1
108090000284 Pepsin A Proteins 0.000 description 1
108091093037 Peptide nucleic acid Proteins 0.000 description 1
102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 1
108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 1
108091000080 Phosphotransferase Proteins 0.000 description 1
101100413173 Phytolacca americana PAP2 gene Proteins 0.000 description 1
241000276498 Pollachius virens Species 0.000 description 1
229920000954 Polyglycolide Polymers 0.000 description 1
241001505332 Polyomavirus sp. Species 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
201000010769 Prader-Willi syndrome Diseases 0.000 description 1
208000006664 Precursor Cell Lymphoblastic Leukemia-Lymphoma Diseases 0.000 description 1
102000001253 Protein Kinase Human genes 0.000 description 1
241000588769 Proteus <enterobacteria> Species 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
241000589517 Pseudomonas aeruginosa Species 0.000 description 1
108010011939 Pyruvate Decarboxylase Proteins 0.000 description 1
102000013009 Pyruvate Kinase Human genes 0.000 description 1
108020005115 Pyruvate Kinase Proteins 0.000 description 1
239000012980 RPMI-1640 medium Substances 0.000 description 1
102000004879 Racemases and epimerases Human genes 0.000 description 1
108090001066 Racemases and epimerases Proteins 0.000 description 1
102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 description 1
101710100968 Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 description 1
102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
108700008625 Reporter Genes Proteins 0.000 description 1
241000223252 Rhodotorula Species 0.000 description 1
108091028664 Ribonucleotide Proteins 0.000 description 1
241000607142 Salmonella Species 0.000 description 1
241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
241000235347 Schizosaccharomyces pombe Species 0.000 description 1
241000311088 Schwanniomyces Species 0.000 description 1
241001123650 Schwanniomyces occidentalis Species 0.000 description 1
241000607768 Shigella Species 0.000 description 1
241000700584 Simplexvirus Species 0.000 description 1
102100038803 Somatotropin Human genes 0.000 description 1
238000002105 Southern blotting Methods 0.000 description 1
241000256248 Spodoptera Species 0.000 description 1
241000256251 Spodoptera frugiperda Species 0.000 description 1
241000269319 Squalius cephalus Species 0.000 description 1
108010090804 Streptavidin Proteins 0.000 description 1
241000187747 Streptomyces Species 0.000 description 1
241000282887 Suidae Species 0.000 description 1
108091008874 T cell receptors Proteins 0.000 description 1
102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
102100027213 T-cell-specific surface glycoprotein CD28 Human genes 0.000 description 1
210000001744 T-lymphocyte Anatomy 0.000 description 1
WDLRUFUQRNWCPK-UHFFFAOYSA-N Tetraxetan Chemical compound OC(=O)CN1CCN(CC(O)=O)CCN(CC(O)=O)CCN(CC(O)=O)CC1 WDLRUFUQRNWCPK-UHFFFAOYSA-N 0.000 description 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
239000004473 Threonine Substances 0.000 description 1
108010022394 Threonine synthase Proteins 0.000 description 1
102000006601 Thymidine Kinase Human genes 0.000 description 1
108020004440 Thymidine kinase Proteins 0.000 description 1
108010034949 Thyroglobulin Proteins 0.000 description 1
102000009843 Thyroglobulin Human genes 0.000 description 1
241001149964 Tolypocladium Species 0.000 description 1
101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
241000223259 Trichoderma Species 0.000 description 1
102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 1
108700015934 Triose-phosphate isomerases Proteins 0.000 description 1
101710162629 Trypsin inhibitor Proteins 0.000 description 1
229940122618 Trypsin inhibitor Drugs 0.000 description 1
102000004243 Tubulin Human genes 0.000 description 1
108090000704 Tubulin Proteins 0.000 description 1
208000026928 Turner syndrome Diseases 0.000 description 1
108091034131 VA RNA Proteins 0.000 description 1
244000000188 Vaccinium ovalifolium Species 0.000 description 1
240000001866 Vernicia fordii Species 0.000 description 1
108010003533 Viral Envelope Proteins Proteins 0.000 description 1
208000036142 Viral infection Diseases 0.000 description 1
102100038968 WAP four-disulfide core domain protein 1 Human genes 0.000 description 1
241000235013 Yarrowia Species 0.000 description 1
HMNZFMSWFCAGGW-XPWSMXQVSA-N [3-[hydroxy(2-hydroxyethoxy)phosphoryl]oxy-2-[(e)-octadec-9-enoyl]oxypropyl] (e)-octadec-9-enoate Chemical compound CCCCCCCC\C=C\CCCCCCCC(=O)OCC(COP(O)(=O)OCCO)OC(=O)CCCCCCC\C=C\CCCCCCCC HMNZFMSWFCAGGW-XPWSMXQVSA-N 0.000 description 1
230000003187 abdominal effect Effects 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
230000021736 acetylation Effects 0.000 description 1
238000006640 acetylation reaction Methods 0.000 description 1
239000003929 acidic solution Substances 0.000 description 1
230000036982 action potential Effects 0.000 description 1
239000011149 active material Substances 0.000 description 1
239000013543 active substance Substances 0.000 description 1
230000001154 acute effect Effects 0.000 description 1
230000010933 acylation Effects 0.000 description 1
238000005917 acylation reaction Methods 0.000 description 1
230000006978 adaptation Effects 0.000 description 1
239000000654 additive Substances 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
238000012867 alanine scanning Methods 0.000 description 1
150000001299 aldehydes Chemical class 0.000 description 1
PPQRONHOSHZGFQ-LMVFSUKVSA-N aldehydo-D-ribose 5-phosphate Chemical group OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PPQRONHOSHZGFQ-LMVFSUKVSA-N 0.000 description 1
230000002152 alkylating effect Effects 0.000 description 1
230000029936 alkylation Effects 0.000 description 1
238000005804 alkylation reaction Methods 0.000 description 1
WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
108010026331 alpha-Fetoproteins Proteins 0.000 description 1
108010001818 alpha-sarcin Proteins 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
239000003708 ampul Substances 0.000 description 1
238000012443 analytical study Methods 0.000 description 1
238000004873 anchoring Methods 0.000 description 1
238000010171 animal model Methods 0.000 description 1
238000000137 annealing Methods 0.000 description 1
230000000259 anti-tumor effect Effects 0.000 description 1
239000002518 antifoaming agent Substances 0.000 description 1
229940041181 antineoplastic drug Drugs 0.000 description 1
239000002830 appetite depressant Substances 0.000 description 1
239000013011 aqueous formulation Substances 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
208000011775 arteriosclerosis disease Diseases 0.000 description 1
125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 1
238000013542 behavioral therapy Methods 0.000 description 1
102000006635 beta-lactamase Human genes 0.000 description 1
GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
239000011230 binding agent Substances 0.000 description 1
238000004166 bioassay Methods 0.000 description 1
229920002988 biodegradable polymer Polymers 0.000 description 1
239000004621 biodegradable polymer Substances 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
230000008827 biological function Effects 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
230000006287 biotinylation Effects 0.000 description 1
238000007413 biotinylation Methods 0.000 description 1
210000002459 blastocyst Anatomy 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000008280 blood Substances 0.000 description 1
230000036765 blood level Effects 0.000 description 1
238000010241 blood sampling Methods 0.000 description 1
210000001124 body fluid Anatomy 0.000 description 1
239000010839 body fluid Substances 0.000 description 1
230000036760 body temperature Effects 0.000 description 1
238000006664 bond formation reaction Methods 0.000 description 1
IXIBAKNTJSCKJM-BUBXBXGNSA-N bovine insulin Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)C(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 IXIBAKNTJSCKJM-BUBXBXGNSA-N 0.000 description 1
238000009395 breeding Methods 0.000 description 1
230000001488 breeding effect Effects 0.000 description 1
230000005587 bubbling Effects 0.000 description 1
239000008366 buffered solution Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
239000011575 calcium Substances 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
230000000711 cancerogenic effect Effects 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 1
229960003669 carbenicillin Drugs 0.000 description 1
125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
231100000315 carcinogenic Toxicity 0.000 description 1
238000012219 cassette mutagenesis Methods 0.000 description 1
239000003729 cation exchange resin Substances 0.000 description 1
230000033366 cell cycle process Effects 0.000 description 1
230000007910 cell fusion Effects 0.000 description 1
230000022534 cell killing Effects 0.000 description 1
239000013592 cell lysate Substances 0.000 description 1
230000010307 cell transformation Effects 0.000 description 1
210000002421 cell wall Anatomy 0.000 description 1
230000002032 cellular defenses Effects 0.000 description 1
230000030570 cellular localization Effects 0.000 description 1
125000003636 chemical group Chemical group 0.000 description 1
238000007385 chemical modification Methods 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
235000012000 cholesterol Nutrition 0.000 description 1
238000011098 chromatofocusing Methods 0.000 description 1
239000003593 chromogenic compound Substances 0.000 description 1
238000003200 chromosome mapping Methods 0.000 description 1
230000001684 chronic effect Effects 0.000 description 1
229960002173 citrulline Drugs 0.000 description 1
235000013477 citrulline Nutrition 0.000 description 1
238000005352 clarification Methods 0.000 description 1
239000013599 cloning vector Substances 0.000 description 1
101150074451 clpP gene Proteins 0.000 description 1
101150043719 clpP1 gene Proteins 0.000 description 1
101150102296 clpP2 gene Proteins 0.000 description 1
230000004186 co-expression Effects 0.000 description 1
238000000749 co-immunoprecipitation Methods 0.000 description 1
238000005354 coacervation Methods 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
239000008139 complexing agent Substances 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
230000001143 conditioned effect Effects 0.000 description 1
230000001268 conjugating effect Effects 0.000 description 1
238000010276 construction Methods 0.000 description 1
238000013270 controlled release Methods 0.000 description 1
208000029078 coronary artery disease Diseases 0.000 description 1
239000003431 cross linking reagent Substances 0.000 description 1
239000011243 crosslinked material Substances 0.000 description 1
239000013078 crystal Substances 0.000 description 1
DMSZORWOGDLWGN-UHFFFAOYSA-N ctk1a3526 Chemical compound NP(N)(N)=O DMSZORWOGDLWGN-UHFFFAOYSA-N 0.000 description 1
210000004748 cultured cell Anatomy 0.000 description 1
UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 description 1
230000009089 cytolysis Effects 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
231100000433 cytotoxic Toxicity 0.000 description 1
230000001472 cytotoxic effect Effects 0.000 description 1
230000006240 deamidation Effects 0.000 description 1
238000000354 decomposition reaction Methods 0.000 description 1
230000007123 defense Effects 0.000 description 1
230000007812 deficiency Effects 0.000 description 1
230000022811 deglycosylation Effects 0.000 description 1
230000003413 degradative effect Effects 0.000 description 1
230000003111 delayed effect Effects 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
238000001212 derivatisation Methods 0.000 description 1
230000001627 detrimental effect Effects 0.000 description 1
235000014113 dietary fatty acids Nutrition 0.000 description 1
238000001085 differential centrifugation Methods 0.000 description 1
238000002050 diffraction method Methods 0.000 description 1
102000004419 dihydrofolate reductase Human genes 0.000 description 1
125000005442 diisocyanate group Chemical group 0.000 description 1
238000007865 diluting Methods 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
239000004205 dimethyl polysiloxane Substances 0.000 description 1
206010013023 diphtheria Diseases 0.000 description 1
HSLYYGWJRDSBBS-UHFFFAOYSA-L disodium bis(sulfanylidene)-disulfido-lambda6-sulfane Chemical compound S(=S)(=S)([S-])[S-].[Na+].[Na+] HSLYYGWJRDSBBS-UHFFFAOYSA-L 0.000 description 1
238000009826 distribution Methods 0.000 description 1
ZWIBGKZDAWNIFC-UHFFFAOYSA-N disuccinimidyl suberate Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCCC(=O)ON1C(=O)CCC1=O ZWIBGKZDAWNIFC-UHFFFAOYSA-N 0.000 description 1
125000004119 disulfanediyl group Chemical group *SS* 0.000 description 1
PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
229960004679 doxorubicin Drugs 0.000 description 1
238000009509 drug development Methods 0.000 description 1
238000001035 drying Methods 0.000 description 1
230000009977 dual effect Effects 0.000 description 1
230000000062 effect on obesity Effects 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
238000010828 elution Methods 0.000 description 1
230000012202 endocytosis Effects 0.000 description 1
230000002616 endonucleolytic effect Effects 0.000 description 1
239000002158 endotoxin Substances 0.000 description 1
238000005265 energy consumption Methods 0.000 description 1
108010028531 enomycin Proteins 0.000 description 1
239000000147 enterotoxin Substances 0.000 description 1
231100000655 enterotoxin Toxicity 0.000 description 1
230000007515 enzymatic degradation Effects 0.000 description 1
230000006862 enzymatic digestion Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
235000020774 essential nutrients Nutrition 0.000 description 1
230000032050 esterification Effects 0.000 description 1
238000005886 esterification reaction Methods 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
238000001704 evaporation Methods 0.000 description 1
238000002270 exclusion chromatography Methods 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000001400 expression cloning Methods 0.000 description 1
239000013613 expression plasmid Substances 0.000 description 1
239000004744 fabric Substances 0.000 description 1
210000003195 fascia Anatomy 0.000 description 1
235000013861 fat-free Nutrition 0.000 description 1
229930195729 fatty acid Natural products 0.000 description 1
239000000194 fatty acid Substances 0.000 description 1
150000004665 fatty acids Chemical class 0.000 description 1
239000012091 fetal bovine serum Substances 0.000 description 1
239000012894 fetal calf serum Substances 0.000 description 1
230000001605 fetal effect Effects 0.000 description 1
239000000706 filtrate Substances 0.000 description 1
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
150000002222 fluorine compounds Chemical class 0.000 description 1
239000012537 formulation buffer Substances 0.000 description 1
238000011990 functional testing Methods 0.000 description 1
229930182830 galactose Natural products 0.000 description 1
238000012239 gene modification Methods 0.000 description 1
238000012252 genetic analysis Methods 0.000 description 1
208000016361 genetic disease Diseases 0.000 description 1
230000008303 genetic mechanism Effects 0.000 description 1
230000005017 genetic modification Effects 0.000 description 1
235000013617 genetically modified food Nutrition 0.000 description 1
108060003196 globin Proteins 0.000 description 1
102000018146 globin Human genes 0.000 description 1
238000007446 glucose tolerance test Methods 0.000 description 1
229960002989 glutamic acid Drugs 0.000 description 1
125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 description 1
230000002414 glycolytic effect Effects 0.000 description 1
229930182470 glycoside Natural products 0.000 description 1
150000002338 glycosides Chemical class 0.000 description 1
239000000122 growth hormone Substances 0.000 description 1
239000003966 growth inhibitor Substances 0.000 description 1
208000019622 heart disease Diseases 0.000 description 1
125000000623 heterocyclic group Chemical group 0.000 description 1
239000000833 heterodimer Substances 0.000 description 1
235000014304 histidine Nutrition 0.000 description 1
125000000487 histidyl group Chemical class [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
229920001600 hydrophobic polymer Polymers 0.000 description 1
238000012872 hydroxylapatite chromatography Methods 0.000 description 1
230000033444 hydroxylation Effects 0.000 description 1
238000005805 hydroxylation reaction Methods 0.000 description 1
229920003063 hydroxymethyl cellulose Polymers 0.000 description 1
229940031574 hydroxymethyl cellulose Drugs 0.000 description 1
229960002591 hydroxyproline Drugs 0.000 description 1
125000001841 imino group Chemical group [H]N=* 0.000 description 1
230000014726 immortalization of host cell Effects 0.000 description 1
210000004201 immune sera Anatomy 0.000 description 1
229940042743 immune sera Drugs 0.000 description 1
210000000987 immune system Anatomy 0.000 description 1
230000000984 immunochemical effect Effects 0.000 description 1
230000005847 immunogenicity Effects 0.000 description 1
239000012535 impurity Substances 0.000 description 1
238000012750 in vivo screening Methods 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
150000002484 inorganic compounds Chemical class 0.000 description 1
229910010272 inorganic material Inorganic materials 0.000 description 1
229960003786 inosine Drugs 0.000 description 1
229940125396 insulin Drugs 0.000 description 1
230000010354 integration Effects 0.000 description 1
239000000138 intercalating agent Substances 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
229940079322 interferon Drugs 0.000 description 1
239000000543 intermediate Substances 0.000 description 1
238000001361 intraarterial administration Methods 0.000 description 1
238000007918 intramuscular administration Methods 0.000 description 1
238000007912 intraperitoneal administration Methods 0.000 description 1
239000007928 intraperitoneal injection Substances 0.000 description 1
238000010253 intravenous injection Methods 0.000 description 1
230000026045 iodination Effects 0.000 description 1
238000006192 iodination reaction Methods 0.000 description 1
238000005342 ion exchange Methods 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
229960000318 kanamycin Drugs 0.000 description 1
229930027917 kanamycin Natural products 0.000 description 1
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
229930182823 kanamycin A Natural products 0.000 description 1
108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 1
JJTUDXZGHPGLLC-UHFFFAOYSA-N lactide Chemical compound CC1OC(=O)C(C)OC1=O JJTUDXZGHPGLLC-UHFFFAOYSA-N 0.000 description 1
239000008101 lactose Substances 0.000 description 1
150000002611 lead compounds Chemical class 0.000 description 1
229960004338 leuprorelin Drugs 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
235000019421 lipase Nutrition 0.000 description 1
238000001638 lipofection Methods 0.000 description 1
238000009630 liquid culture Methods 0.000 description 1
230000005923 long-lasting effect Effects 0.000 description 1
235000020845 low-calorie diet Nutrition 0.000 description 1
101150074251 lpp gene Proteins 0.000 description 1
210000005265 lung cell Anatomy 0.000 description 1
210000001165 lymph node Anatomy 0.000 description 1
230000002934 lysing effect Effects 0.000 description 1
230000002101 lytic effect Effects 0.000 description 1
210000001161 mammalian embryo Anatomy 0.000 description 1
238000002844 melting Methods 0.000 description 1
230000008018 melting Effects 0.000 description 1
210000004779 membrane envelope Anatomy 0.000 description 1
229960003151 mercaptamine Drugs 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
229960000485 methotrexate Drugs 0.000 description 1
DFTAZNAEBRBBKP-UHFFFAOYSA-N methyl 4-sulfanylbutanimidate Chemical compound COC(=N)CCCS DFTAZNAEBRBBKP-UHFFFAOYSA-N 0.000 description 1
230000011987 methylation Effects 0.000 description 1
238000007069 methylation reaction Methods 0.000 description 1
239000004530 micro-emulsion Substances 0.000 description 1
235000013336 milk Nutrition 0.000 description 1
239000008267 milk Substances 0.000 description 1
210000004080 milk Anatomy 0.000 description 1
239000003226 mitogen Substances 0.000 description 1
239000000178 monomer Substances 0.000 description 1
229940035032 monophosphoryl lipid a Drugs 0.000 description 1
210000000663 muscle cell Anatomy 0.000 description 1
230000000869 mutational effect Effects 0.000 description 1
239000002088 nanocapsule Substances 0.000 description 1
239000002105 nanoparticle Substances 0.000 description 1
229960004927 neomycin Drugs 0.000 description 1
230000006855 networking Effects 0.000 description 1
230000001537 neural effect Effects 0.000 description 1
239000002547 new drug Substances 0.000 description 1
102000042567 non-coding RNA Human genes 0.000 description 1
235000021590 normal diet Nutrition 0.000 description 1
238000001668 nucleic acid synthesis Methods 0.000 description 1
239000002777 nucleoside Substances 0.000 description 1
125000003835 nucleoside group Chemical group 0.000 description 1
229920002113 octoxynol Polymers 0.000 description 1
229940046166 oligodeoxynucleotide Drugs 0.000 description 1
229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
238000011275 oncology therapy Methods 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
235000020830 overeating Nutrition 0.000 description 1
239000006174 pH buffer Substances 0.000 description 1
238000004091 panning Methods 0.000 description 1
229940055729 papain Drugs 0.000 description 1
235000019834 papain Nutrition 0.000 description 1
229950009506 penicillinase Drugs 0.000 description 1
229940111202 pepsin Drugs 0.000 description 1
239000000816 peptidomimetic Substances 0.000 description 1
125000001151 peptidyl group Chemical group 0.000 description 1
230000000737 periodic effect Effects 0.000 description 1
210000005259 peripheral blood Anatomy 0.000 description 1
239000011886 peripheral blood Substances 0.000 description 1
238000002823 phage display Methods 0.000 description 1
230000000144 pharmacologic effect Effects 0.000 description 1
238000001050 pharmacotherapy Methods 0.000 description 1
108010076042 phenomycin Proteins 0.000 description 1
NMHMNPHRMNGLLB-UHFFFAOYSA-N phloretic acid Chemical compound OC(=O)CCC1=CC=C(O)C=C1 NMHMNPHRMNGLLB-UHFFFAOYSA-N 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
150000003904 phospholipids Chemical class 0.000 description 1
102000020233 phosphotransferase Human genes 0.000 description 1
239000000902 placebo Substances 0.000 description 1
229940068196 placebo Drugs 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
230000008488 polyadenylation Effects 0.000 description 1
229920000728 polyester Polymers 0.000 description 1
229920002338 polyhydroxyethylmethacrylate Polymers 0.000 description 1
239000004926 polymethyl methacrylate Substances 0.000 description 1
108010055896 polyornithine Proteins 0.000 description 1
229920002714 polyornithine Polymers 0.000 description 1
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
229920001451 polypropylene glycol Polymers 0.000 description 1
229920001296 polysiloxane Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
238000011176 pooling Methods 0.000 description 1
239000013641 positive control Substances 0.000 description 1
230000008092 positive effect Effects 0.000 description 1
230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 description 1
230000004481 post-translational protein modification Effects 0.000 description 1
238000004321 preservation Methods 0.000 description 1
230000002265 prevention Effects 0.000 description 1
230000003449 preventive effect Effects 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
230000035755 proliferation Effects 0.000 description 1
230000000644 propagated effect Effects 0.000 description 1
230000000069 prophylactic effect Effects 0.000 description 1
QLNJFJADRCOGBJ-UHFFFAOYSA-N propionamide Chemical compound CCC(N)=O QLNJFJADRCOGBJ-UHFFFAOYSA-N 0.000 description 1
235000019419 proteases Nutrition 0.000 description 1
230000001681 protective effect Effects 0.000 description 1
230000004952 protein activity Effects 0.000 description 1
108020001580 protein domains Proteins 0.000 description 1
230000006916 protein interaction Effects 0.000 description 1
108060006633 protein kinase Proteins 0.000 description 1
229940116540 protein supplement Drugs 0.000 description 1
235000005974 protein supplement Nutrition 0.000 description 1
238000001243 protein synthesis Methods 0.000 description 1
230000002797 proteolythic effect Effects 0.000 description 1
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
210000001938 protoplast Anatomy 0.000 description 1
230000005855 radiation Effects 0.000 description 1
150000003254 radicals Chemical class 0.000 description 1
230000009257 reactivity Effects 0.000 description 1
230000010837 receptor-mediated endocytosis Effects 0.000 description 1
238000010188 recombinant method Methods 0.000 description 1
208000017443 reproductive system disease Diseases 0.000 description 1
230000000979 retarding effect Effects 0.000 description 1
230000002207 retinal effect Effects 0.000 description 1
238000004007 reversed phase HPLC Methods 0.000 description 1
238000012552 review Methods 0.000 description 1
PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
150000003290 ribose derivatives Chemical group 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000013391 scatchard analysis Methods 0.000 description 1
238000000926 separation method Methods 0.000 description 1
150000003355 serines Chemical class 0.000 description 1
125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
210000000717 sertoli cell Anatomy 0.000 description 1
230000035939 shock Effects 0.000 description 1
239000000377 silicon dioxide Substances 0.000 description 1
238000002741 site-directed mutagenesis Methods 0.000 description 1
210000004894 snout Anatomy 0.000 description 1
PTLRDCMBXHILCL-UHFFFAOYSA-M sodium arsenite Chemical compound [Na+].[O-][As]=O PTLRDCMBXHILCL-UHFFFAOYSA-M 0.000 description 1
239000012064 sodium phosphate buffer Substances 0.000 description 1
235000010265 sodium sulphite Nutrition 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
230000003381 solubilizing effect Effects 0.000 description 1
238000010186 staining Methods 0.000 description 1
238000011146 sterile filtration Methods 0.000 description 1
150000003431 steroids Chemical class 0.000 description 1
230000004936 stimulating effect Effects 0.000 description 1
210000001768 subcellular fraction Anatomy 0.000 description 1
238000007920 subcutaneous administration Methods 0.000 description 1
238000010254 subcutaneous injection Methods 0.000 description 1
230000008093 supporting effect Effects 0.000 description 1
238000011477 surgical intervention Methods 0.000 description 1
230000004083 survival effect Effects 0.000 description 1
238000004114 suspension culture Methods 0.000 description 1
208000001608 teratocarcinoma Diseases 0.000 description 1
238000006365 thiocyanation reaction Methods 0.000 description 1
CNHYKKNIIGEXAY-UHFFFAOYSA-N thiolan-2-imine Chemical compound N=C1CCCS1 CNHYKKNIIGEXAY-UHFFFAOYSA-N 0.000 description 1
RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
229940113082 thymine Drugs 0.000 description 1
229960002175 thyroglobulin Drugs 0.000 description 1
230000036964 tight binding Effects 0.000 description 1
RUELTTOHQODFPA-UHFFFAOYSA-N toluene 2,6-diisocyanate Chemical compound CC1=C(N=C=O)C=CC=C1N=C=O RUELTTOHQODFPA-UHFFFAOYSA-N 0.000 description 1
238000011200 topical administration Methods 0.000 description 1
FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
108091006106 transcriptional activators Proteins 0.000 description 1
239000012096 transfection reagent Substances 0.000 description 1
238000000844 transformation Methods 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
230000014621 translational initiation Effects 0.000 description 1
108091005703 transmembrane proteins Proteins 0.000 description 1
102000035160 transmembrane proteins Human genes 0.000 description 1
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
229940038773 trisodium citrate Drugs 0.000 description 1
239000002753 trypsin inhibitor Substances 0.000 description 1
210000004881 tumor cell Anatomy 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
150000003668 tyrosines Chemical class 0.000 description 1
230000009452 underexpressoin Effects 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
229940035893 uracil Drugs 0.000 description 1
230000035899 viability Effects 0.000 description 1
108700026220 vif Genes Proteins 0.000 description 1
230000009385 viral infection Effects 0.000 description 1
238000003260 vortexing Methods 0.000 description 1
230000003313 weakening effect Effects 0.000 description 1
230000004580 weight loss Effects 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1