DE60009575T2 - Nachweis von bakterien und bakteriophagen mit immobilisierten enzymsubstraten - Google Patents
Nachweis von bakterien und bakteriophagen mit immobilisierten enzymsubstraten Download PDFInfo
- Publication number
- DE60009575T2 DE60009575T2 DE60009575T DE60009575T DE60009575T2 DE 60009575 T2 DE60009575 T2 DE 60009575T2 DE 60009575 T DE60009575 T DE 60009575T DE 60009575 T DE60009575 T DE 60009575T DE 60009575 T2 DE60009575 T2 DE 60009575T2
- Authority
- DE
- Germany
- Prior art keywords
- phage
- reaction mixture
- bacteria
- sample
- enzyme substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- 238000000034 method Methods 0.000 claims abstract description 45
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- IEJPPSMHUUQABK-UHFFFAOYSA-N 2,4-diphenyl-4h-1,3-oxazol-5-one Chemical group O=C1OC(C=2C=CC=CC=2)=NC1C1=CC=CC=C1 IEJPPSMHUUQABK-UHFFFAOYSA-N 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 11
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- PJUPKRYGDFTMTM-UHFFFAOYSA-N 1-hydroxybenzotriazole;hydrate Chemical compound O.C1=CC=C2N(O)N=NC2=C1 PJUPKRYGDFTMTM-UHFFFAOYSA-N 0.000 description 1
- VQKDJSXHVSAIAR-UHFFFAOYSA-N 1h-imidazol-2-yl carbamate Chemical class NC(=O)OC1=NC=CN1 VQKDJSXHVSAIAR-UHFFFAOYSA-N 0.000 description 1
- RHCSKNNOAZULRK-APZFVMQVSA-N 2,2-dideuterio-2-(3,4,5-trimethoxyphenyl)ethanamine Chemical compound NCC([2H])([2H])C1=CC(OC)=C(OC)C(OC)=C1 RHCSKNNOAZULRK-APZFVMQVSA-N 0.000 description 1
- PQZLOWONDMXBIN-UHFFFAOYSA-N 2-(1-adamantyl)oxirane Chemical class C1OC1C1(C2)CC(C3)CC2CC3C1 PQZLOWONDMXBIN-UHFFFAOYSA-N 0.000 description 1
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- 239000006137 Luria-Bertani broth Substances 0.000 description 1
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- 229940053200 antiepileptics fatty acid derivative Drugs 0.000 description 1
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- UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/20—Coumarin derivatives
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US548157 | 1990-07-05 | ||
| US09/548,157 US6436661B1 (en) | 2000-04-13 | 2000-04-13 | Bacteria and bacteriophage detection using immobilized enzyme substrates |
| PCT/US2000/022277 WO2001079528A1 (en) | 2000-04-13 | 2000-08-14 | Bacteria and bacteriophage detection using immobilized enzyme substrates |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60009575D1 DE60009575D1 (de) | 2004-05-06 |
| DE60009575T2 true DE60009575T2 (de) | 2004-12-30 |
Family
ID=24187649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60009575T Expired - Fee Related DE60009575T2 (de) | 2000-04-13 | 2000-08-14 | Nachweis von bakterien und bakteriophagen mit immobilisierten enzymsubstraten |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6436661B1 (https=) |
| EP (1) | EP1272658B1 (https=) |
| JP (1) | JP2003530859A (https=) |
| AT (1) | ATE263253T1 (https=) |
| AU (1) | AU2000269056A1 (https=) |
| CA (1) | CA2405732A1 (https=) |
| DE (1) | DE60009575T2 (https=) |
| WO (1) | WO2001079528A1 (https=) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6436661B1 (en) * | 2000-04-13 | 2002-08-20 | 3M Innovative Properties Company | Bacteria and bacteriophage detection using immobilized enzyme substrates |
| PT1356080E (pt) * | 2001-02-01 | 2006-09-29 | Profos Ag | Deteccao e identificacao de grupos bacterianos |
| US20020192676A1 (en) * | 2001-06-18 | 2002-12-19 | Madonna Angelo J. | Method for determining if a type of bacteria is present in a mixture |
| CA2353307A1 (fr) * | 2001-07-13 | 2003-01-13 | Carmen Parent | Appareil et procede pour le traitement des effluents gazeux |
| CA2474159A1 (en) * | 2002-01-23 | 2003-05-21 | Carl R. Merril | Method for determining sensitivity to a bacteriophage |
| US8216780B2 (en) * | 2002-04-12 | 2012-07-10 | Microphage (Tm) Incorporated | Method for enhanced sensitivity in bacteriophage-based diagnostic assays |
| US20050003346A1 (en) * | 2002-04-12 | 2005-01-06 | Colorado School Of Mines | Apparatus and method for detecting microscopic living organisms using bacteriophage |
| JP4490112B2 (ja) * | 2002-04-12 | 2010-06-23 | コロラド・スクール・オブ・マインズ | 標的細菌性細胞を感染させるためにファージを使用する低濃度の標的細菌を検出するための方法 |
| AU2003284228A1 (en) * | 2002-10-15 | 2004-05-04 | Dwight L. Anderson | Assays to detect or quantify bacterial or viral pathogens and contaminants |
| EP1613965A2 (en) | 2003-04-10 | 2006-01-11 | Kent J. Voorhees | Apparatus and method for detecting microscopic living organisms using bacteriophage |
| US20090286225A1 (en) * | 2004-02-13 | 2009-11-19 | Microphagetm Incorporated | Method and apparatus for bacteriophage-based diagnostic assays |
| US20050250096A1 (en) * | 2004-02-13 | 2005-11-10 | Microphage, Incorporated | Microorganism detection using bacteriophage amplification |
| WO2008157384A2 (en) | 2007-06-15 | 2008-12-24 | Microphage Incorporated | Method of detection of microorganisms with enhanced bacteriophage amplification |
| PL1812585T3 (pl) | 2004-11-01 | 2015-03-31 | Erber Ag | Bakteriofagi jako czynniki selekcyjne |
| WO2006105504A1 (en) * | 2005-03-31 | 2006-10-05 | Microphage Incorporated | Apparatus and method for detecting microorganisms using flagged bacteriophage |
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2000
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- 2000-08-14 EP EP00957436A patent/EP1272658B1/en not_active Expired - Lifetime
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- 2000-08-14 DE DE60009575T patent/DE60009575T2/de not_active Expired - Fee Related
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- 2000-08-14 AU AU2000269056A patent/AU2000269056A1/en not_active Abandoned
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| CA2405732A1 (en) | 2001-10-25 |
| US20030017449A1 (en) | 2003-01-23 |
| EP1272658A1 (en) | 2003-01-08 |
| AU2000269056A1 (en) | 2001-10-30 |
| EP1272658B1 (en) | 2004-03-31 |
| WO2001079528A1 (en) | 2001-10-25 |
| DE60009575D1 (de) | 2004-05-06 |
| JP2003530859A (ja) | 2003-10-21 |
| ATE263253T1 (de) | 2004-04-15 |
| US6436661B1 (en) | 2002-08-20 |
| US6833251B2 (en) | 2004-12-21 |
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