DE3509238A1 - Stabilisierung der aktivitaet von peroxidase in loesung - Google Patents

Stabilisierung der aktivitaet von peroxidase in loesung

Info

Publication number
DE3509238A1
DE3509238A1 DE19853509238 DE3509238A DE3509238A1 DE 3509238 A1 DE3509238 A1 DE 3509238A1 DE 19853509238 DE19853509238 DE 19853509238 DE 3509238 A DE3509238 A DE 3509238A DE 3509238 A1 DE3509238 A1 DE 3509238A1
Authority
DE
Germany
Prior art keywords
solution
phenol
activity
peroxidase
pod
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19853509238
Other languages
German (de)
English (en)
Inventor
Gerlinde 8131 Bernried Deutsch
Dagmar Dr.rer.nat. 8132 Tutzing Klenner
Johann Dr.phil. 8000 München Mattersberger
Rainer Dr.rer.nat. 8035 Gauting Wehner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to DE19853509238 priority Critical patent/DE3509238A1/de
Priority to US06/835,895 priority patent/US4764468A/en
Priority to JP61053825A priority patent/JPS61212286A/ja
Priority to EP86103434A priority patent/EP0196518B1/de
Priority to DE8686103434T priority patent/DE3676527D1/de
Priority to AT86103434T priority patent/ATE59406T1/de
Publication of DE3509238A1 publication Critical patent/DE3509238A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE19853509238 1985-03-14 1985-03-14 Stabilisierung der aktivitaet von peroxidase in loesung Withdrawn DE3509238A1 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE19853509238 DE3509238A1 (de) 1985-03-14 1985-03-14 Stabilisierung der aktivitaet von peroxidase in loesung
US06/835,895 US4764468A (en) 1985-03-14 1986-03-04 Process for stabilizing the activity of peroxidase in solution
JP61053825A JPS61212286A (ja) 1985-03-14 1986-03-13 溶液中のペルオキシダーゼの活性を安定化する方法
EP86103434A EP0196518B1 (de) 1985-03-14 1986-03-14 Stabilisierung der Aktivität von Peroxidase in Lösung
DE8686103434T DE3676527D1 (de) 1985-03-14 1986-03-14 Stabilisierung der aktivitaet von peroxidase in loesung.
AT86103434T ATE59406T1 (de) 1985-03-14 1986-03-14 Stabilisierung der aktivitaet von peroxidase in loesung.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853509238 DE3509238A1 (de) 1985-03-14 1985-03-14 Stabilisierung der aktivitaet von peroxidase in loesung

Publications (1)

Publication Number Publication Date
DE3509238A1 true DE3509238A1 (de) 1986-09-18

Family

ID=6265244

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19853509238 Withdrawn DE3509238A1 (de) 1985-03-14 1985-03-14 Stabilisierung der aktivitaet von peroxidase in loesung
DE8686103434T Expired - Lifetime DE3676527D1 (de) 1985-03-14 1986-03-14 Stabilisierung der aktivitaet von peroxidase in loesung.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8686103434T Expired - Lifetime DE3676527D1 (de) 1985-03-14 1986-03-14 Stabilisierung der aktivitaet von peroxidase in loesung.

Country Status (5)

Country Link
US (1) US4764468A (https=)
EP (1) EP0196518B1 (https=)
JP (1) JPS61212286A (https=)
AT (1) ATE59406T1 (https=)
DE (2) DE3509238A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4874692A (en) * 1987-07-20 1989-10-17 Eastman Kodak Company Binder composition and analytical element having stabilized peroxidase in layer containing the composition
JPS6463380A (en) * 1987-09-03 1989-03-09 Teijin Ltd Stabilized peroxidase composition
US5188955A (en) * 1987-10-28 1993-02-23 Gds Technology, Inc. Method of preserving arylacylamidase in aqueous solution
FR2630457B1 (fr) * 1988-04-20 1990-08-31 Anda Biolog Utilisation de l'acide para-amino-salicylique pour la stabilisation de l'activite enzymatique de la peroxydase en solution, agent stabilisant et solution stabilisee en comportant application
JP2727112B2 (ja) * 1988-04-26 1998-03-11 コニカ株式会社 安定なペルオキシダーゼ組成物及び安定な抗体組成物
US4992372A (en) * 1989-02-02 1991-02-12 Pokora Alexander R Method for removing impurities from peroxidase solutions
DE4037764A1 (de) * 1990-11-28 1992-06-04 Behringwerke Ag Waschloesung fuer festphasen-immunometrische verfahren, die stabilisatoren fuer das markierungssystem enthaelt und ihre verwendung
US5460944A (en) * 1991-10-28 1995-10-24 Boehringer Mannheim Gmbh Storable protein solution
JP3345507B2 (ja) * 1994-04-20 2002-11-18 第一化学薬品株式会社 アシアログリコプロテインレセプターの測定法及びこれに用いる測定試薬
WO2006076034A2 (en) * 2004-05-21 2006-07-20 Bhanu, Kalra Stabilized two component system for chemilumiescent assay in immunodiagnostics

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2913553C2 (de) * 1979-04-04 1981-09-17 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und Reagenz zur enzymatischen Bestimmung von Enzymsubstraten
US4378429A (en) * 1979-08-23 1983-03-29 Modrovich Ivan Endre Enzymatic method and stabilized solutions for determining total cholesterol in human serum
US4290773A (en) * 1979-11-13 1981-09-22 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4252896A (en) * 1980-01-07 1981-02-24 Abbott Laboratories Method of stabilizing peroxidase in a serum protein based medium
JPS5783287A (en) * 1980-11-14 1982-05-25 Kyowa Hakko Kogyo Co Ltd Elimination of hydrogen peroxide
DE3046241A1 (de) * 1980-12-08 1982-07-15 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zur bestimmung von cholesterin
DE3261832D1 (en) * 1981-07-30 1985-02-21 Hoffmann La Roche Method for the stabilisation of peroxidase in a liquid medium, and the stabilised medium so obtained
US4521511A (en) * 1982-09-22 1985-06-04 Enzyme Technology Company Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations
US4504579A (en) * 1982-11-08 1985-03-12 Abbott Laboratories Stabilized peroxidase compositions
DE3463395D1 (en) * 1983-02-11 1987-06-04 Nat Res Dev Enhanced luminescent or luminometric assay
JPS60192260A (ja) * 1984-03-13 1985-09-30 Denka Seiken Co Ltd 安定化パ−オキシダ−ゼ標識抗ヒトＩｇＧ（γ）
JPS6178385A (ja) * 1984-09-25 1986-04-21 Toyobo Co Ltd 安定なペルオキシダ−ゼ組成物

Also Published As

Publication number Publication date
JPS61212286A (ja) 1986-09-20
US4764468A (en) 1988-08-16
EP0196518B1 (de) 1990-12-27
JPH0243472B2 (https=) 1990-09-28
EP0196518A1 (de) 1986-10-08
ATE59406T1 (de) 1991-01-15
DE3676527D1 (de) 1991-02-07

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Legal Events

Date Code Title Description
8141 Disposal/no request for examination