DE3112441A1 - Verfahren und reagenz zur endotoxin-bestimmung - Google Patents
Verfahren und reagenz zur endotoxin-bestimmungInfo
- Publication number
- DE3112441A1 DE3112441A1 DE19813112441 DE3112441A DE3112441A1 DE 3112441 A1 DE3112441 A1 DE 3112441A1 DE 19813112441 DE19813112441 DE 19813112441 DE 3112441 A DE3112441 A DE 3112441A DE 3112441 A1 DE3112441 A1 DE 3112441A1
- Authority
- DE
- Germany
- Prior art keywords
- endotoxin
- inhibition
- lysate
- reducing agent
- hydrolyzed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002158 endotoxin Substances 0.000 title claims description 56
- 238000000034 method Methods 0.000 title claims description 17
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 9
- 239000006166 lysate Substances 0.000 claims description 51
- 239000000243 solution Substances 0.000 claims description 49
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 29
- 230000005764 inhibitory process Effects 0.000 claims description 26
- 239000003638 chemical reducing agent Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 150000001768 cations Chemical class 0.000 claims description 13
- 239000011780 sodium chloride Substances 0.000 claims description 13
- 229920001577 copolymer Polymers 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 12
- -1 alkali metal salts Chemical class 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 230000035945 sensitivity Effects 0.000 claims description 10
- 229920002125 Sokalan® Polymers 0.000 claims description 9
- 239000004584 polyacrylic acid Substances 0.000 claims description 9
- 229910052783 alkali metal Inorganic materials 0.000 claims description 8
- 239000011575 calcium Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 229920002401 polyacrylamide Polymers 0.000 claims description 8
- 229920002239 polyacrylonitrile Polymers 0.000 claims description 8
- 159000000000 sodium salts Chemical class 0.000 claims description 8
- 239000002960 lipid emulsion Substances 0.000 claims description 7
- 108010058237 plasma protein fraction Proteins 0.000 claims description 7
- 229940081857 plasma protein fraction Drugs 0.000 claims description 7
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 6
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims description 5
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- RNIHAPSVIGPAFF-UHFFFAOYSA-N Acrylamide-acrylic acid resin Chemical compound NC(=O)C=C.OC(=O)C=C RNIHAPSVIGPAFF-UHFFFAOYSA-N 0.000 claims description 4
- 241000239218 Limulus Species 0.000 claims description 4
- 229920006322 acrylamide copolymer Polymers 0.000 claims description 4
- 150000003863 ammonium salts Chemical class 0.000 claims description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 4
- 102100026735 Coagulation factor VIII Human genes 0.000 claims description 3
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 claims description 3
- 108010071390 Serum Albumin Proteins 0.000 claims description 3
- 102000007562 Serum Albumin Human genes 0.000 claims description 3
- 238000000338 in vitro Methods 0.000 claims description 3
- 238000010998 test method Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 3
- 150000001340 alkali metals Chemical class 0.000 claims 3
- 229910052791 calcium Inorganic materials 0.000 claims 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 2
- 229910052749 magnesium Inorganic materials 0.000 claims 2
- 239000011777 magnesium Substances 0.000 claims 2
- BPKIGYQJPYCAOW-FFJTTWKXSA-I calcium;potassium;disodium;(2s)-2-hydroxypropanoate;dichloride;dihydroxide;hydrate Chemical compound O.[OH-].[OH-].[Na+].[Na+].[Cl-].[Cl-].[K+].[Ca+2].C[C@H](O)C([O-])=O BPKIGYQJPYCAOW-FFJTTWKXSA-I 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 229920000642 polymer Polymers 0.000 description 17
- 239000000523 sample Substances 0.000 description 8
- 239000008227 sterile water for injection Substances 0.000 description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 150000002500 ions Chemical class 0.000 description 6
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000004108 freeze drying Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229940117913 acrylamide Drugs 0.000 description 4
- 239000003146 anticoagulant agent Substances 0.000 description 4
- 229940127219 anticoagulant drug Drugs 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 229920000867 polyelectrolyte Polymers 0.000 description 4
- 150000003839 salts Chemical group 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 241000239220 Limulus polyphemus Species 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 150000001408 amides Chemical class 0.000 description 3
- 210000000087 hemolymph Anatomy 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000001252 acrylic acid derivatives Chemical class 0.000 description 2
- 125000004103 aminoalkyl group Chemical group 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OXIKLRTYAYRAOE-CMDGGOBGSA-N (e)-3-(1-benzyl-3-pyridin-3-ylpyrazol-4-yl)prop-2-enoic acid Chemical compound N1=C(C=2C=NC=CC=2)C(/C=C/C(=O)O)=CN1CC1=CC=CC=C1 OXIKLRTYAYRAOE-CMDGGOBGSA-N 0.000 description 1
- OEPOKWHJYJXUGD-UHFFFAOYSA-N 2-(3-phenylmethoxyphenyl)-1,3-thiazole-4-carbaldehyde Chemical compound O=CC1=CSC(C=2C=C(OCC=3C=CC=CC=3)C=CC=2)=N1 OEPOKWHJYJXUGD-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 229920002126 Acrylic acid copolymer Polymers 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001529572 Chaceon affinis Species 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 239000012480 LAL reagent Substances 0.000 description 1
- HBBGRARXTFLTSG-UHFFFAOYSA-N Lithium ion Chemical compound [Li+] HBBGRARXTFLTSG-UHFFFAOYSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- GYCMBHHDWRMZGG-UHFFFAOYSA-N Methylacrylonitrile Chemical compound CC(=C)C#N GYCMBHHDWRMZGG-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- XTXRWKRVRITETP-UHFFFAOYSA-N Vinyl acetate Chemical compound CC(=O)OC=C XTXRWKRVRITETP-UHFFFAOYSA-N 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- VEUACKUBDLVUAC-UHFFFAOYSA-N [Na].[Ca] Chemical compound [Na].[Ca] VEUACKUBDLVUAC-UHFFFAOYSA-N 0.000 description 1
- 150000001253 acrylic acids Chemical class 0.000 description 1
- 229910001413 alkali metal ion Inorganic materials 0.000 description 1
- 125000003545 alkoxy group Chemical group 0.000 description 1
- 150000005215 alkyl ethers Chemical class 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- XYLMUPLGERFSHI-UHFFFAOYSA-N alpha-Methylstyrene Chemical compound CC(=C)C1=CC=CC=C1 XYLMUPLGERFSHI-UHFFFAOYSA-N 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 239000003914 blood derivative Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 125000002843 carboxylic acid group Chemical group 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 150000001805 chlorine compounds Chemical class 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000012525 endotoxin sample Substances 0.000 description 1
- GFJVXXWOPWLRNU-UHFFFAOYSA-N ethenyl formate Chemical compound C=COC=O GFJVXXWOPWLRNU-UHFFFAOYSA-N 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000008240 homogeneous mixture Substances 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 150000002430 hydrocarbons Chemical group 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229940028435 intralipid Drugs 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 238000012454 limulus amebocyte lysate test Methods 0.000 description 1
- 229910001416 lithium ion Inorganic materials 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 125000002560 nitrile group Chemical group 0.000 description 1
- 239000006259 organic additive Substances 0.000 description 1
- 125000000962 organic group Chemical group 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- KUKFKAPJCRZILJ-UHFFFAOYSA-N prop-2-enenitrile;prop-2-enoic acid Chemical compound C=CC#N.OC(=O)C=C KUKFKAPJCRZILJ-UHFFFAOYSA-N 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 239000008215 water for injection Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/135,810 US4273557A (en) | 1980-03-31 | 1980-03-31 | Limulus lysate procedure for determining endotoxins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3112441A1 true DE3112441A1 (de) | 1982-02-18 |
| DE3112441C2 DE3112441C2 (en, 2012) | 1989-12-07 |
Family
ID=22469790
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19813112441 Granted DE3112441A1 (de) | 1980-03-31 | 1981-03-28 | Verfahren und reagenz zur endotoxin-bestimmung |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US4273557A (en, 2012) |
| JP (1) | JPS56154661A (en, 2012) |
| AU (1) | AU541871B2 (en, 2012) |
| BE (1) | BE888213A (en, 2012) |
| CA (1) | CA1160549A (en, 2012) |
| CH (1) | CH658517A5 (en, 2012) |
| DE (1) | DE3112441A1 (en, 2012) |
| FR (1) | FR2479262A1 (en, 2012) |
| GB (1) | GB2073415B (en, 2012) |
| IT (1) | IT1144806B (en, 2012) |
| NL (1) | NL191340C (en, 2012) |
| SE (1) | SE445588B (en, 2012) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0121868A1 (en) * | 1983-04-01 | 1984-10-17 | Mitsubishi Rayon Co., Ltd. | Endotoxin-detecting device |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4322217A (en) * | 1980-06-27 | 1982-03-30 | Mallinckrodt, Inc. | Process for preparing Limulus lysate |
| US4476093A (en) * | 1980-09-30 | 1984-10-09 | The Green Cross Corporation | Kit for preparing sample for use in endotoxin test |
| US4414336A (en) * | 1981-01-28 | 1983-11-08 | The Green Cross Corporation | Method for preparing sample for use in endotoxin test |
| JPS5885162A (ja) * | 1981-11-16 | 1983-05-21 | Seikagaku Kogyo Co Ltd | エンドトキシン測定法 |
| DE3439761A1 (de) * | 1984-10-31 | 1986-05-07 | Klaus-Peter 6800 Mannheim Becker | Verfahren zur bestimmung von endotoxinkonzentrationen |
| US5594113A (en) * | 1988-06-23 | 1997-01-14 | Associates Of Cape Cod, Inc. | Endotoxin binding and neutralizing protein and uses thereof |
| AU3772289A (en) | 1988-06-23 | 1990-01-12 | Associates Of Cape Cod, Inc. | Endotoxin binding protein and uses thereof |
| JP3242733B2 (ja) * | 1993-02-26 | 2001-12-25 | 生化学工業株式会社 | エンドトキシン特異的測定剤 |
| US5637474A (en) * | 1994-10-31 | 1997-06-10 | Wako Pure Chemical Industries, Ltd. | Process for measuring amount of endotoxin or (1→3)-β-D-glucan by kinetic turbidimetric method |
| US6270982B1 (en) | 1997-10-09 | 2001-08-07 | Charles River Laboratories | Methods and compositions for the detection of bacterial endotoxins |
| DE19931276C2 (de) * | 1999-07-07 | 2002-10-24 | Hans-Martin Seipp | Verfahren zur Herstellung von Probenkörpern mit Biofilmbeladung und Verfahren zur Quantifizierung der Beladung von Probenkörpern mit Biofilm sowie die Verwendung der mit dem Herstellverfahren geschaffenen Probenkörpern |
| US6391295B1 (en) | 1999-10-25 | 2002-05-21 | Associates Of Cape Cod, Inc. | Artificial bait |
| EP1606414B1 (en) | 2003-03-17 | 2009-12-23 | Charles River Laboratories, Inc. | Methods and compositions for the detection of microbial contaminants |
| US20050048655A1 (en) * | 2003-04-18 | 2005-03-03 | Novitsky Thomas J. | Kit for detecting endotoxin |
| EP1627040A2 (en) * | 2003-04-18 | 2006-02-22 | Associates Of Cape Cod, Inc. | Kit for detecting endotoxin in aqueous solutions |
| US7968280B2 (en) * | 2004-12-02 | 2011-06-28 | Charles River Laboratories, Inc. | Methods for the detection and/or quantification of gram positive bacterial contaminants |
| JP5096166B2 (ja) | 2005-01-13 | 2012-12-12 | チャールズ リバー ラボラトリーズ， インコーポレイテッド | 生体サンプル中の微生物を分類する方法 |
| BRPI0610274A2 (pt) | 2005-05-30 | 2010-06-08 | Daiichi Sankyo Co Ltd | método de teste para endotoxina |
| US20090286692A1 (en) * | 2008-04-15 | 2009-11-19 | Wainwright Norman R | Cartridge and Method for Sample Analysis |
| MY155541A (en) * | 2010-10-29 | 2015-10-30 | Universiti Malaysia Terengganu | Process for the preparation of amoebocyte lysate from the haemolymph of the horseshoe crab |
| US11061015B2 (en) | 2015-08-28 | 2021-07-13 | Sharp Life Science (Eu) Limited | Droplet microfluidic device and methods of sensing the results of an assay therein |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4107077A (en) * | 1975-07-14 | 1978-08-15 | Associates Of Cape Cod, Inc. | Limulus lysate of improved sensitivity and preparing the same |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2625471A (en) * | 1952-02-20 | 1953-01-13 | Monsanto Chemicals | Fertilizing compositions |
| US4096091A (en) * | 1974-07-17 | 1978-06-20 | Baxter Travenol Laboratories, Inc. | Limulus lysate having improved capacity to precipitate in the presence of low endotoxin concentrations, and reconstituting solutions therefor |
| US3959128A (en) * | 1974-12-27 | 1976-05-25 | Preventive Systems, Inc | Process for removing endotoxin from biological fluids |
| US3944391A (en) * | 1974-12-27 | 1976-03-16 | Preventive Systems, Inc. | In vitro process for detecting endotoxin in a biological fluid |
| US4038029A (en) * | 1976-01-20 | 1977-07-26 | Worthington Biochemical Corporation | Limulus lysate turbidity test for pyrogens |
| GB1522127A (en) | 1976-07-23 | 1978-08-23 | Mallinckrodt Inc | Limulus amebocyte lysate reagent compositions |
-
1980
- 1980-03-31 US US06/135,810 patent/US4273557A/en not_active Expired - Lifetime
-
1981
- 1981-03-27 GB GB8109637A patent/GB2073415B/en not_active Expired
- 1981-03-28 DE DE19813112441 patent/DE3112441A1/de active Granted
- 1981-03-30 FR FR8106293A patent/FR2479262A1/fr active Granted
- 1981-03-30 AU AU68916/81A patent/AU541871B2/en not_active Ceased
- 1981-03-30 CA CA000374179A patent/CA1160549A/en not_active Expired
- 1981-03-30 NL NL8101561A patent/NL191340C/xx not_active IP Right Cessation
- 1981-03-30 CH CH2149/81A patent/CH658517A5/de not_active IP Right Cessation
- 1981-03-30 SE SE8102001A patent/SE445588B/sv not_active IP Right Cessation
- 1981-03-31 IT IT67446/81A patent/IT1144806B/it active
- 1981-03-31 BE BE0/204327A patent/BE888213A/fr not_active IP Right Cessation
- 1981-03-31 JP JP4836081A patent/JPS56154661A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4107077A (en) * | 1975-07-14 | 1978-08-15 | Associates Of Cape Cod, Inc. | Limulus lysate of improved sensitivity and preparing the same |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0121868A1 (en) * | 1983-04-01 | 1984-10-17 | Mitsubishi Rayon Co., Ltd. | Endotoxin-detecting device |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3112441C2 (en, 2012) | 1989-12-07 |
| CH658517A5 (de) | 1986-11-14 |
| NL191340B (nl) | 1995-01-02 |
| FR2479262A1 (fr) | 1981-10-02 |
| GB2073415A (en) | 1981-10-14 |
| CA1160549A (en) | 1984-01-17 |
| AU6891681A (en) | 1981-10-08 |
| AU541871B2 (en) | 1985-01-24 |
| BE888213A (fr) | 1981-07-16 |
| SE445588B (sv) | 1986-06-30 |
| GB2073415B (en) | 1983-12-21 |
| NL191340C (nl) | 1995-06-01 |
| JPS648789B2 (en, 2012) | 1989-02-15 |
| US4273557A (en) | 1981-06-16 |
| FR2479262B1 (en, 2012) | 1983-03-04 |
| SE8102001L (sv) | 1981-10-01 |
| IT8167446A0 (it) | 1981-03-31 |
| JPS56154661A (en) | 1981-11-30 |
| IT1144806B (it) | 1986-10-29 |
| NL8101561A (nl) | 1981-10-16 |
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