DE2953449A1 - Verfahren und ausruestung fuer die enzym-immunoassay von biologischen substanzen - Google Patents
Verfahren und ausruestung fuer die enzym-immunoassay von biologischen substanzenInfo
- Publication number
- DE2953449A1 DE2953449A1 DE19792953449 DE2953449A DE2953449A1 DE 2953449 A1 DE2953449 A1 DE 2953449A1 DE 19792953449 DE19792953449 DE 19792953449 DE 2953449 A DE2953449 A DE 2953449A DE 2953449 A1 DE2953449 A1 DE 2953449A1
- Authority
- DE
- Germany
- Prior art keywords
- enzyme
- biological substance
- avidin
- biotin
- dehydrogenase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 55
- OROGSEYTTFOCAN-DNJOTXNNSA-N codeine Chemical compound C([C@H]1[C@H](N(CC[C@@]112)C)C3)=C[C@H](O)[C@@H]1OC1=C2C3=CC=C1OC OROGSEYTTFOCAN-DNJOTXNNSA-N 0.000 claims description 32
- 108090001008 Avidin Proteins 0.000 claims description 29
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- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 claims description 24
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 claims description 24
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- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 claims 1
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- 239000007900 aqueous suspension Substances 0.000 description 2
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- 230000015572 biosynthetic process Effects 0.000 description 2
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- KWTSXDURSIMDCE-QMMMGPOBSA-N (S)-amphetamine Chemical compound C[C@H](N)CC1=CC=CC=C1 KWTSXDURSIMDCE-QMMMGPOBSA-N 0.000 description 1
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 1
- PCDWFBFHIIKIPM-UHFFFAOYSA-N 3-ethyl-2h-1,3-benzothiazole-2-sulfonic acid Chemical compound C1=CC=C2N(CC)C(S(O)(=O)=O)SC2=C1 PCDWFBFHIIKIPM-UHFFFAOYSA-N 0.000 description 1
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 1
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 1
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 1
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- QLALYHDPUIEJKC-UHFFFAOYSA-N ON1C(CCC1=O)=O.C(CCC(=O)O)(=O)O.ON1C(CCC1=O)=O Chemical compound ON1C(CCC1=O)=O.C(CCC(=O)O)(=O)O.ON1C(CCC1=O)=O QLALYHDPUIEJKC-UHFFFAOYSA-N 0.000 description 1
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- NMHMNPHRMNGLLB-UHFFFAOYSA-N phloretic acid Chemical compound OC(=O)CCC1=CC=C(O)C=C1 NMHMNPHRMNGLLB-UHFFFAOYSA-N 0.000 description 1
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- 229910052708 sodium Inorganic materials 0.000 description 1
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- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- BEOOHQFXGBMRKU-UHFFFAOYSA-N sodium cyanoborohydride Chemical compound [Na+].[B-]C#N BEOOHQFXGBMRKU-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
- G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1774978 | 1978-05-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2953449A1 true DE2953449A1 (de) | 1981-06-04 |
Family
ID=10100551
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19792953449 Ceased DE2953449A1 (de) | 1978-05-04 | 1979-05-03 | Verfahren und ausruestung fuer die enzym-immunoassay von biologischen substanzen |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US4298685A (enrdf_load_stackoverflow) |
| EP (1) | EP0005271A3 (enrdf_load_stackoverflow) |
| JP (1) | JPS5510590A (enrdf_load_stackoverflow) |
| DE (1) | DE2953449A1 (enrdf_load_stackoverflow) |
| DK (1) | DK149908C (enrdf_load_stackoverflow) |
| FR (1) | FR2458072A1 (enrdf_load_stackoverflow) |
| GB (1) | GB2055851A (enrdf_load_stackoverflow) |
| NL (1) | NL7915050A (enrdf_load_stackoverflow) |
Families Citing this family (125)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4446231A (en) * | 1979-10-03 | 1984-05-01 | Self Colin H | Immunoassay using an amplified cyclic detection system |
| GB2059421A (en) * | 1979-10-03 | 1981-04-23 | Self C H | Assay method and reagents therefor |
| US4656252A (en) * | 1980-01-24 | 1987-04-07 | Giese Roger W | Amidobiotin compounds useful in a avidin-biotin multiple layering process |
| FR2486657A1 (fr) * | 1980-07-09 | 1982-01-15 | Pasteur Institut | Procede de detection et de dosage d'une substance biologique par erythroadsorption |
| NL8102178A (nl) * | 1981-05-02 | 1982-12-01 | Stichting Centraal Lab | Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze. |
| US4684609A (en) * | 1981-06-15 | 1987-08-04 | Vector Laboratories, Inc. | Method and substance for the enhanced labelling of cellular material |
| AU8594382A (en) * | 1981-08-05 | 1983-02-10 | F. Hoffmann-La Roche Ag | Immunoassay using biotin-avidin-enzyme complex |
| US4395486A (en) * | 1981-08-19 | 1983-07-26 | Medical College Of Ga. Research Inst., Inc. | Method for the direct analysis of sickle cell anemia |
| DE3263249D1 (en) * | 1981-08-21 | 1985-05-30 | Hoffmann La Roche | Method for the determination of carcinoembryonic antigen (cea) and suitable antibody solution for the determination |
| US4444878A (en) * | 1981-12-21 | 1984-04-24 | Boston Biomedical Research Institute, Inc. | Bispecific antibody determinants |
| US4506009A (en) * | 1982-03-30 | 1985-03-19 | University Of California | Heterogeneous immunoassay method |
| US4535057A (en) * | 1982-07-26 | 1985-08-13 | Amf Incorporated | Immunoassay employing monoclonal herpes simplex antibody and biotin-avidin detection system |
| US4530900A (en) * | 1982-09-13 | 1985-07-23 | Seragen Diagnostics Inc. | Soluble insoluble polymers in enzymeimmunoassay |
| GB8307156D0 (en) * | 1983-03-15 | 1983-04-20 | Celltech Ltd | Heterogeneous binding assays |
| US4496654A (en) * | 1983-04-08 | 1985-01-29 | Quidel | Detection of HCG with solid phase support having avidin coating |
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| US4582810A (en) * | 1983-09-30 | 1986-04-15 | Becton, Dickinson And Company | Immuno-agglutination particle suspensions |
| EP0170652A4 (en) * | 1984-01-27 | 1988-08-23 | Molecular Biosystems Inc | TEST FOR IMMOBILIZED REPORTER GROUPS. |
| US4943523A (en) * | 1984-01-30 | 1990-07-24 | Enzo Biochem, Inc. | Detectable molecules, method of preparation and use |
| US4849505A (en) * | 1984-01-30 | 1989-07-18 | Enzo Biochem, Inc. | Detectable molecules, method of preparation and use |
| US5002885A (en) * | 1984-01-30 | 1991-03-26 | Enzo Biochem, Inc. | Detectable molecules, method preparation and use |
| AU3844485A (en) * | 1984-02-09 | 1985-08-15 | Enzo Biochem Inc. | Heterologous detection of cabeled dna |
| US4704354A (en) * | 1984-02-15 | 1987-11-03 | University Of Cincinnati | Virion assay method for use in in vitro screening of teratogens and carcinogens |
| CA1260372A (en) * | 1984-04-27 | 1989-09-26 | Elazar Rabbani | Hybridization method for the detection of genetic materials |
| US4632901A (en) * | 1984-05-11 | 1986-12-30 | Hybritech Incorporated | Method and apparatus for immunoassays |
| JPS60252265A (ja) * | 1984-05-29 | 1985-12-12 | Toray Ind Inc | 発光試薬およびそれを用いる生物学的に活性な物質の測定法 |
| GB8422452D0 (en) * | 1984-09-05 | 1984-10-10 | Serono Diagnostics Ltd | Assay |
| US5310687A (en) * | 1984-10-31 | 1994-05-10 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US4692403A (en) * | 1984-11-28 | 1987-09-08 | The Texas A&M University System | Methods and compositions for the detection of acquired immune deficiency syndrome |
| US4737453A (en) * | 1984-12-12 | 1988-04-12 | Immunomedics, Inc. | Sandwich immunoassay utilizing a separation specific binding substance |
| US4704355A (en) * | 1985-03-27 | 1987-11-03 | New Horizons Diagnostics Corporation | Assay utilizing ATP encapsulated within liposome particles |
| US4935339A (en) * | 1985-05-07 | 1990-06-19 | Nichols Institute Diagnostics | Delayed solid phase immunologic assay |
| US4801532A (en) * | 1985-08-15 | 1989-01-31 | Wake Forest University | Immunological methods for diagnosing neurocysticercosis |
| US4740456A (en) * | 1985-08-15 | 1988-04-26 | Wake Forest University | Immunological methods for diagnosing neurocysticercosis |
| US5073341A (en) * | 1985-08-21 | 1991-12-17 | Biotope, Inc. | Devices for conducting specific binding assays |
| JPH0614041B2 (ja) * | 1985-09-18 | 1994-02-23 | 帝人株式会社 | 肺表面活性物質の検出方法及びそれに用いる試薬キツト |
| US4806631A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on solvolyzed nylon supports |
| US4806546A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on derivatized nylon supports |
| US4933275A (en) * | 1985-10-24 | 1990-06-12 | The General Hospital Corporation | Method for the detection of a polypeptide subunit in the presence of a quaternary protein containing the subunit |
| US5501949A (en) * | 1985-12-10 | 1996-03-26 | Murex Diagnostics Corporation | Particle bound binding component immunoassay |
| US5262334A (en) * | 1986-01-30 | 1993-11-16 | Fred Hutchinson Cancer Research Center | Method for immunoselection of cells using avidin and biotin |
| US5225353A (en) * | 1986-01-30 | 1993-07-06 | Fred Hutchinson Cancer Research Center | Method for immunoselection of cells using avidin and biotin |
| WO1987004794A1 (en) * | 1986-02-06 | 1987-08-13 | Microbiological Associates, Inc. | Latex agglutination using avidin/biotin system |
| US4772550A (en) * | 1986-02-10 | 1988-09-20 | Miles Inc. | Heterogeneous specific binding assay employing an aggregatable binding reagent |
| FR2601455B1 (fr) * | 1986-07-11 | 1989-08-04 | Stallergenes Lab | Nouveaux reactifs biologiques en phase solide et leur procede d'obtention |
| US4959307A (en) * | 1986-09-05 | 1990-09-25 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
| US5085988A (en) * | 1986-09-05 | 1992-02-04 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
| US5001048A (en) * | 1987-06-05 | 1991-03-19 | Aurthur D. Little, Inc. | Electrical biosensor containing a biological receptor immobilized and stabilized in a protein film |
| US5192507A (en) * | 1987-06-05 | 1993-03-09 | Arthur D. Little, Inc. | Receptor-based biosensors |
| IL82873A0 (en) * | 1987-06-15 | 1987-12-20 | Orgenics Ltd | Reversed competitive solid phase immunoassay for detecting single epitope analytes and kit therefor |
| US5534620A (en) * | 1987-09-30 | 1996-07-09 | Beckman Instruments, Inc. | Method of heterogenous purification using a bidentate conjugate |
| US5196351A (en) * | 1987-09-30 | 1993-03-23 | Beckman Instruments, Inc. | Bidentate conjugate and method of use thereof |
| EP0310361A3 (en) * | 1987-09-30 | 1989-05-24 | Beckman Instruments, Inc. | Tridentate conjugate and method of use thereof |
| US4859612A (en) * | 1987-10-07 | 1989-08-22 | Hygeia Sciences, Inc. | Metal sol capture immunoassay procedure, kit for use therewith and captured metal containing composite |
| ATE91023T1 (de) * | 1987-12-01 | 1993-07-15 | Biotope Inc | Verfahren und vorrichtungen zur durchfuehrung von untersuchungen. |
| CA1312277C (en) * | 1987-12-18 | 1993-01-05 | Richard C. Sutton | Avidin- and biotin-immobilized reagents, analytical elements and methods of use |
| US4870007A (en) * | 1987-12-18 | 1989-09-26 | Eastman Kodak Company | Immobilized biotinylated receptor in test device, kit and method for determining a ligand |
| US5459080A (en) * | 1988-01-29 | 1995-10-17 | Abbott Laboratories | Ion-capture assays using a specific binding member conjugated to carboxymethylamylose |
| US5866322A (en) * | 1988-01-29 | 1999-02-02 | Abbott Laboratories | Method for performing Rubella assay |
| US5459078A (en) * | 1988-01-29 | 1995-10-17 | Abbott Laboratories | Methods and reagents for performing ion-capture digoxin assays |
| US6326136B1 (en) | 1988-04-01 | 2001-12-04 | Digene Corporation | Macromolecular conjugate made using unsaturated aldehydes |
| US5362624A (en) * | 1988-05-25 | 1994-11-08 | Boehringer Mannheim Gmbh | Process for the determination of an immunologically detectable substance and a suitable reaction vessel therefor |
| IE64286B1 (en) * | 1988-05-25 | 1995-07-26 | Boehringer Mannheim Gmbh | Process for the determination of an immunologically detectable substance and a suitable vessel therefor |
| DE3822750A1 (de) * | 1988-07-05 | 1990-01-11 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung einer spezifisch bindefaehigen substanz |
| DE3907651A1 (de) * | 1988-07-08 | 1990-01-11 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung von antikoerpern |
| DE3829245A1 (de) * | 1988-08-29 | 1990-03-01 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung einer spezifisch bindefaehigen substanz |
| US5888728A (en) | 1988-10-17 | 1999-03-30 | Molecular Devices Corporation | Hapten derivatized capture membrane and diagnostic assays using such membrane |
| EP0390910B1 (en) | 1988-10-17 | 1995-12-13 | Molecular Devices Corporation | Hapten derivatized capture membran and assays using such membrane |
| DE3836348A1 (de) * | 1988-10-25 | 1990-04-26 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung von fuer ein antigen klassenspezifischen antikoerpern und dazu geeignetes reagenz |
| JPH04502107A (ja) * | 1988-12-07 | 1992-04-16 | ザ・ジェネラル・ホスピタル・コーポレーション | 挿入を有するかまたは欠失に相当するｄｎａの豊富化およびクローニングの方法 |
| US5939272A (en) * | 1989-01-10 | 1999-08-17 | Biosite Diagnostics Incorporated | Non-competitive threshold ligand-receptor assays |
| US5028535A (en) * | 1989-01-10 | 1991-07-02 | Biosite Diagnostics, Inc. | Threshold ligand-receptor assay |
| CA2008100A1 (en) * | 1989-01-20 | 1990-07-20 | Hubert Bayer | Method for the determination of immunologically detectable substance |
| JP2619549B2 (ja) * | 1989-06-29 | 1997-06-11 | 日本商事株式会社 | 抗原の定量法およびそれに用いる固相 |
| JPH0350138A (ja) * | 1989-07-18 | 1991-03-04 | Canon Inc | 光学ガラス |
| US5512659A (en) * | 1989-08-04 | 1996-04-30 | Syntex (U.S.A.) Inc. | Compositions useful in heterogeneous immunoassays |
| DE69032383T2 (de) * | 1989-08-04 | 1998-12-10 | Behringwerke AG, 35041 Marburg | Heterogene Bindungstests |
| US5176999A (en) * | 1989-12-07 | 1993-01-05 | Eastman Kodak Company | Buffered wash composition, insolubilizing composition, test kits and method of use |
| US5180828A (en) * | 1990-02-09 | 1993-01-19 | Molecular Devices Corporation | Chromophoric reagents for incorporation of biotin or other haptens into macromolecules |
| DE4006054A1 (de) * | 1990-02-26 | 1991-08-29 | Boehringer Mannheim Gmbh | Verfahren zur immunologischen bestimmung von liganden |
| US5922615A (en) | 1990-03-12 | 1999-07-13 | Biosite Diagnostics Incorporated | Assay devices comprising a porous capture membrane in fluid-withdrawing contact with a nonabsorbent capillary network |
| US5143852A (en) * | 1990-09-14 | 1992-09-01 | Biosite Diagnostics, Inc. | Antibodies to ligand analogues and their utility in ligand-receptor assays |
| JP2968039B2 (ja) * | 1990-11-29 | 1999-10-25 | 株式会社京都第一科学 | 化学発光免疫測定法 |
| IE920778A1 (en) * | 1991-03-12 | 1992-09-23 | Du Pont | Method for specific binding assays using a releasable ligand |
| EP0507587A3 (en) * | 1991-04-03 | 1993-03-03 | Syntex (U.S.A.) Inc. | Immunoassay for immunoglubulins |
| JPH05133952A (ja) * | 1991-04-03 | 1993-05-28 | Syntex Usa Inc | イムノグロブリン類のイムノアツセイ |
| US5998220A (en) | 1991-05-29 | 1999-12-07 | Beckman Coulter, Inc. | Opposable-element assay devices, kits, and methods employing them |
| US6168956B1 (en) | 1991-05-29 | 2001-01-02 | Beckman Coulter, Inc. | Multiple component chromatographic assay device |
| US5877028A (en) | 1991-05-29 | 1999-03-02 | Smithkline Diagnostics, Inc. | Immunochromatographic assay device |
| US5464749A (en) * | 1991-07-22 | 1995-11-07 | Bayer Corporation | Immunoassay of free substances in biological fluids |
| US5726010A (en) * | 1991-07-31 | 1998-03-10 | Idexx Laboratories, Inc. | Reversible flow chromatographic binding assay |
| US6007999A (en) * | 1991-07-31 | 1999-12-28 | Idexx Laboratories, Inc. | Reversible flow chromatographic binding assay |
| US7713528B1 (en) | 1993-02-18 | 2010-05-11 | Enzo Therapeutics, Inc. | Method for in vivo delivery of active compounds using reagent conjugate |
| US5536820A (en) * | 1993-02-26 | 1996-07-16 | E. I. Du Pont De Nemours And Company | Avidin-binding azo reagents |
| US5677196A (en) * | 1993-05-18 | 1997-10-14 | University Of Utah Research Foundation | Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays |
| US5512492A (en) * | 1993-05-18 | 1996-04-30 | University Of Utah Research Foundation | Waveguide immunosensor with coating chemistry providing enhanced sensitivity |
| US5919712A (en) * | 1993-05-18 | 1999-07-06 | University Of Utah Research Foundation | Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays |
| DE4319506A1 (de) * | 1993-06-12 | 1994-12-15 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis metabolisch markierter DNA |
| US5518882A (en) * | 1993-12-21 | 1996-05-21 | Biotex Laboratories, Inc. | Immunological methods of component selection and recovery |
| IL112372A (en) * | 1994-02-07 | 2001-08-26 | Res Dev Foundation | Non-viral vector for the delivery of genetic information to cells |
| AU1744495A (en) * | 1994-02-15 | 1995-08-29 | T Cell Diagnostics, Inc. | Immunoassay kit comprising universal reagents |
| US5747352A (en) * | 1994-05-23 | 1998-05-05 | Beckman Instruments, Inc. | Reagents and methods for the rapid and quantitative assay of pharmacological agents |
| DE4434093A1 (de) * | 1994-09-23 | 1996-03-28 | Boehringer Mannheim Gmbh | Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz |
| US5814565A (en) * | 1995-02-23 | 1998-09-29 | University Of Utah Research Foundation | Integrated optic waveguide immunosensor |
| AU6720096A (en) | 1995-08-09 | 1997-03-05 | Quidel Corporation | Test strip and method for one step lateral flow assay |
| US5773224A (en) * | 1996-02-12 | 1998-06-30 | Grandics; Peter | Immunoselection system for cell elution |
| EP0988552B1 (en) * | 1997-06-10 | 2010-11-24 | Lpath, Inc. | Methods for early detection of heart disease |
| US7087420B1 (en) | 1997-07-17 | 2006-08-08 | Cambia | Microbial β-glucuronidase genes, gene products and uses thereof |
| US6391547B1 (en) * | 1997-09-09 | 2002-05-21 | Center For The Application Of Molecular Biology To International Agriculture | Microbial β-glucuronidase genes, gene products and uses thereof |
| US6222619B1 (en) | 1997-09-18 | 2001-04-24 | University Of Utah Research Foundation | Diagnostic device and method |
| US6210978B1 (en) * | 1998-03-02 | 2001-04-03 | Bayer Corporation | Method and device for the detection of analyte in a fluid test sample |
| US6153442A (en) * | 1998-05-20 | 2000-11-28 | Dade Behring Inc. | Reagents and methods for specific binding assays |
| US6153394A (en) * | 1998-09-18 | 2000-11-28 | Board Of Trustees Operating Michigan State University | Immunoassay for equine protozoal myeloencephalitis in horses |
| US7262019B2 (en) * | 2001-05-03 | 2007-08-28 | Immunetics, Inc. | System and methods for detection of Bacillus anthracis related analytes in biological fluids |
| WO2002090961A2 (en) * | 2001-05-03 | 2002-11-14 | Immunetics, Inc. | Systems and methods for detection of analytes in biological fluids |
| ES2486269T3 (es) | 2001-10-22 | 2014-08-18 | The Scripps Research Institute | Compuestos que eligen anticuerpos como diana |
| DE60232688D1 (de) * | 2001-11-20 | 2009-07-30 | Univ Duke | Grenzflächen-biomaterialien |
| US7754155B2 (en) * | 2002-03-15 | 2010-07-13 | Ross Amelia A | Devices and methods for isolating target cells |
| US20070134739A1 (en) * | 2005-12-12 | 2007-06-14 | Gyros Patent Ab | Microfluidic assays and microfluidic devices |
| EP2032984A1 (en) | 2005-12-12 | 2009-03-11 | Gyros Patent Ab | Microfluidic assays and microfluidic devices |
| CN101622340A (zh) * | 2006-11-15 | 2010-01-06 | 茵维特罗根戴纳股份公司 | 生物素化合物与支持物可逆结合的方法 |
| JP2016164554A (ja) * | 2015-02-27 | 2016-09-08 | 株式会社リコー | 標的検査装置、標的検査キット、標的検査方法、転写媒体、及び標的検査装置の製造方法 |
| WO2019175745A1 (en) * | 2018-03-13 | 2019-09-19 | Kunaparaju, Venkata Subba Raju | Methods and composition for preparing viscous substrate |
| CA3098779A1 (en) | 2018-05-07 | 2019-11-14 | Immundiagnostik Ag | System for analysing quantitative lateral flow chromatography |
| EP3924735A1 (en) | 2019-02-15 | 2021-12-22 | Immundiagnostik AG | Rapid test for diagnosis of bacterial infections in neonates |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154599B (nl) * | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
| US3852157A (en) * | 1971-05-14 | 1974-12-03 | Syva Corp | Compounds for enzyme amplification assay |
| US3966556A (en) * | 1972-11-06 | 1976-06-29 | Syva Company | Compounds for enzyme amplification assay methadone analogs |
| US4017597A (en) * | 1974-10-30 | 1977-04-12 | Monsanto Company | Unitized solid phase immunoassay kit and method |
| US4134792A (en) * | 1976-12-06 | 1979-01-16 | Miles Laboratories, Inc. | Specific binding assay with an enzyme modulator as a labeling substance |
-
1979
- 1979-05-02 JP JP5466779A patent/JPS5510590A/ja active Pending
- 1979-05-03 US US06/035,619 patent/US4298685A/en not_active Expired - Lifetime
- 1979-05-03 DE DE19792953449 patent/DE2953449A1/de not_active Ceased
- 1979-05-03 DK DK182279A patent/DK149908C/da not_active IP Right Cessation
- 1979-05-03 NL NL7915050A patent/NL7915050A/nl unknown
- 1979-05-03 GB GB8026150A patent/GB2055851A/en not_active Withdrawn
- 1979-05-03 EP EP79101354A patent/EP0005271A3/en not_active Withdrawn
-
1980
- 1980-07-11 FR FR8015865A patent/FR2458072A1/fr active Granted
Non-Patent Citations (1)
| Title |
|---|
| Clin.Chem., Vol. 22, No. 8, 1976, S. 1243-1255 * |
Also Published As
| Publication number | Publication date |
|---|---|
| DK182279A (da) | 1979-11-05 |
| NL7915050A (nl) | 1980-09-30 |
| FR2458072B1 (enrdf_load_stackoverflow) | 1982-05-21 |
| JPS5510590A (en) | 1980-01-25 |
| FR2458072A1 (fr) | 1980-12-26 |
| GB2055851A (en) | 1981-03-11 |
| DK149908C (da) | 1987-03-09 |
| DK149908B (da) | 1986-10-20 |
| EP0005271A3 (en) | 1980-03-19 |
| EP0005271A2 (en) | 1979-11-14 |
| US4298685A (en) | 1981-11-03 |
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| 8141 | Disposal/no request for examination | ||
| 8110 | Request for examination paragraph 44 | ||
| 8131 | Rejection |