DE2839052A1 - Mikrobiologisches verfahren - Google Patents
Mikrobiologisches verfahrenInfo
- Publication number
- DE2839052A1 DE2839052A1 DE19782839052 DE2839052A DE2839052A1 DE 2839052 A1 DE2839052 A1 DE 2839052A1 DE 19782839052 DE19782839052 DE 19782839052 DE 2839052 A DE2839052 A DE 2839052A DE 2839052 A1 DE2839052 A1 DE 2839052A1
- Authority
- DE
- Germany
- Prior art keywords
- gdh
- dna
- phage
- plasmid
- microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims description 24
- 230000002906 microbiologic effect Effects 0.000 title description 3
- 239000013612 plasmid Substances 0.000 claims description 41
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 30
- 230000007246 mechanism Effects 0.000 claims description 29
- 108090000623 proteins and genes Proteins 0.000 claims description 29
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 24
- 244000005700 microbiome Species 0.000 claims description 23
- 239000012634 fragment Substances 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 17
- 102000004169 proteins and genes Human genes 0.000 claims description 15
- 108010029645 galactitol 2-dehydrogenase Proteins 0.000 claims description 13
- 229910021529 ammonia Inorganic materials 0.000 claims description 12
- 230000015572 biosynthetic process Effects 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 10
- 241000588724 Escherichia coli Species 0.000 claims description 9
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 9
- 229930195712 glutamate Natural products 0.000 claims description 9
- 108010027322 single cell proteins Proteins 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 241000589516 Pseudomonas Species 0.000 claims description 7
- 238000003786 synthesis reaction Methods 0.000 claims description 6
- 230000004048 modification Effects 0.000 claims description 5
- 238000012986 modification Methods 0.000 claims description 5
- -1 ammonium ions Chemical class 0.000 claims description 4
- 230000002068 genetic effect Effects 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 241000863393 Methylophilus methylotrophus Species 0.000 claims description 3
- 239000000969 carrier Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 238000012239 gene modification Methods 0.000 claims description 3
- 230000005017 genetic modification Effects 0.000 claims description 3
- 235000013617 genetically modified food Nutrition 0.000 claims description 3
- 239000003550 marker Substances 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 241000588748 Klebsiella Species 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 47
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 45
- 102000016901 Glutamate dehydrogenase Human genes 0.000 description 43
- 108020000311 Glutamate Synthase Proteins 0.000 description 10
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 9
- 102000003960 Ligases Human genes 0.000 description 7
- 108090000364 Ligases Proteins 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000002950 deficient Effects 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 230000037361 pathway Effects 0.000 description 4
- 238000001890 transfection Methods 0.000 description 4
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 3
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000005396 glutamine synthetase Human genes 0.000 description 3
- 108020002326 glutamine synthetase Proteins 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 230000002463 transducing effect Effects 0.000 description 3
- 108010042407 Endonucleases Proteins 0.000 description 2
- 102000004533 Endonucleases Human genes 0.000 description 2
- 241000701959 Escherichia virus Lambda Species 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000007429 general method Methods 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 239000003471 mutagenic agent Substances 0.000 description 2
- 231100000707 mutagenic chemical Toxicity 0.000 description 2
- 230000003505 mutagenic effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- MWBWWFOAEOYUST-UHFFFAOYSA-N 2-aminopurine Chemical compound NC1=NC=C2N=CNC2=N1 MWBWWFOAEOYUST-UHFFFAOYSA-N 0.000 description 1
- LQLQRFGHAALLLE-UHFFFAOYSA-N 5-bromouracil Chemical compound BrC1=CNC(=O)NC1=O LQLQRFGHAALLLE-UHFFFAOYSA-N 0.000 description 1
- AGFIRQJZCNVMCW-UAKXSSHOSA-N 5-bromouridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 AGFIRQJZCNVMCW-UAKXSSHOSA-N 0.000 description 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 241000566194 Methylomonas methanolica Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 241000364057 Peoria Species 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002962 chemical mutagen Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 125000004494 ethyl ester group Chemical group 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- HAWPXGHAZFHHAD-UHFFFAOYSA-N mechlorethamine Chemical class ClCCN(C)CCCl HAWPXGHAZFHHAD-UHFFFAOYSA-N 0.000 description 1
- 229960004961 mechlorethamine Drugs 0.000 description 1
- 229940098779 methanesulfonic acid Drugs 0.000 description 1
- AFVFQIVMOAPDHO-UHFFFAOYSA-N methanesulfonic acid Substances CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 229940085991 phosphate ion Drugs 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB3755977 | 1977-09-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2839052A1 true DE2839052A1 (de) | 1979-03-29 |
Family
ID=10397383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19782839052 Ceased DE2839052A1 (de) | 1977-09-08 | 1978-09-07 | Mikrobiologisches verfahren |
Country Status (6)
| Country | Link |
|---|---|
| JP (2) | JPS5467084A (enrdf_load_stackoverflow) |
| AU (1) | AU525490B2 (enrdf_load_stackoverflow) |
| BE (1) | BE870188A (enrdf_load_stackoverflow) |
| DE (1) | DE2839052A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2402704A1 (enrdf_load_stackoverflow) |
| NL (1) | NL7809125A (enrdf_load_stackoverflow) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4171824A (en) * | 1977-05-23 | 1979-10-23 | Foster Edwin E | Bicyle |
| EP0035831B1 (en) * | 1980-03-07 | 1986-01-08 | Imperial Chemical Industries Plc | Method for making genetically modified microorganisms |
| JPS58115846A (ja) * | 1981-12-28 | 1983-07-09 | Matsushita Electric Ind Co Ltd | 相補型絶縁ゲ−ト型半導体装置 |
| SU1364343A1 (ru) * | 1984-07-13 | 1988-01-07 | Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов | Способ получени человеческого лейкоцитарного интерферона альфа-2 |
| JPS63159098U (enrdf_load_stackoverflow) * | 1987-04-07 | 1988-10-18 | ||
| JPH0586788A (ja) * | 1991-09-26 | 1993-04-06 | Sanwa Shutter Corp | 電動ブラインドシヤツターにおける停電時操作可能な開閉機 |
| WO2011150412A1 (en) | 2010-05-28 | 2011-12-01 | Hunter Douglas Inc. | Architectural opening coverings powered by rotary motors |
| EP2763572A4 (en) | 2011-10-03 | 2015-12-09 | Hunter Douglas | METHOD AND DEVICE FOR CONTROLLING COVER ASSEMBLIES FOR BUILDING OPENINGS |
| CA2828819C (en) | 2012-10-03 | 2020-03-10 | Hunter Douglas Inc. | Methods and apparatus to control an architectural opening covering assembly |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1521032A (en) * | 1974-08-08 | 1978-08-09 | Ici Ltd | Biological treatment |
-
1978
- 1978-09-04 BE BE190240A patent/BE870188A/xx not_active IP Right Cessation
- 1978-09-05 FR FR7825482A patent/FR2402704A1/fr active Granted
- 1978-09-05 AU AU39546/78A patent/AU525490B2/en not_active Expired
- 1978-09-07 DE DE19782839052 patent/DE2839052A1/de not_active Ceased
- 1978-09-07 NL NL7809125A patent/NL7809125A/xx not_active Application Discontinuation
- 1978-09-08 JP JP11057278A patent/JPS5467084A/ja active Granted
-
1982
- 1982-06-24 JP JP57109076A patent/JPS5886076A/ja active Pending
Non-Patent Citations (1)
| Title |
|---|
| NICHTS ERMITTELT * |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2402704A1 (fr) | 1979-04-06 |
| BE870188A (fr) | 1979-03-05 |
| JPS622797B2 (enrdf_load_stackoverflow) | 1987-01-21 |
| AU525490B2 (en) | 1982-11-11 |
| JPS5467084A (en) | 1979-05-30 |
| JPS5886076A (ja) | 1983-05-23 |
| NL7809125A (nl) | 1979-03-12 |
| AU3954678A (en) | 1980-03-13 |
| FR2402704B1 (enrdf_load_stackoverflow) | 1983-08-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OAR | Request for search filed | ||
| OB | Request for examination as to novelty | ||
| OC | Search report available | ||
| 8110 | Request for examination paragraph 44 | ||
| 8131 | Rejection |