DE2709399C3 - Einrichtung zum Messen von Zelleigenschaften - Google Patents

Einrichtung zum Messen von Zelleigenschaften

Info

Publication number
DE2709399C3
DE2709399C3 DE2709399A DE2709399A DE2709399C3 DE 2709399 C3 DE2709399 C3 DE 2709399C3 DE 2709399 A DE2709399 A DE 2709399A DE 2709399 A DE2709399 A DE 2709399A DE 2709399 C3 DE2709399 C3 DE 2709399C3
Authority
DE
Germany
Prior art keywords
measuring points
measuring
knee
light
observation window
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE2709399A
Other languages
German (de)
English (en)
Other versions
DE2709399A1 (de
DE2709399B2 (de
Inventor
Auf Nichtnennung Antrag
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to DE2709399A priority Critical patent/DE2709399C3/de
Priority to CH219878A priority patent/CH626725A5/de
Priority to FR7806137A priority patent/FR2382691A1/fr
Priority to GB8468/78A priority patent/GB1574566A/en
Priority to US05/883,661 priority patent/US4225229A/en
Publication of DE2709399A1 publication Critical patent/DE2709399A1/de
Publication of DE2709399B2 publication Critical patent/DE2709399B2/de
Application granted granted Critical
Publication of DE2709399C3 publication Critical patent/DE2709399C3/de
Expired legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • G01N2021/6419Excitation at two or more wavelengths
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N21/05Flow-through cuvettes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Optical Measuring Cells (AREA)
DE2709399A 1977-03-04 1977-03-04 Einrichtung zum Messen von Zelleigenschaften Expired DE2709399C3 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE2709399A DE2709399C3 (de) 1977-03-04 1977-03-04 Einrichtung zum Messen von Zelleigenschaften
CH219878A CH626725A5 (enExample) 1977-03-04 1978-03-01
FR7806137A FR2382691A1 (fr) 1977-03-04 1978-03-03 Dispositif de mesure de proprietes de cellules
GB8468/78A GB1574566A (en) 1977-03-04 1978-03-03 Cell characterisation apparatus
US05/883,661 US4225229A (en) 1977-03-04 1978-03-06 Apparatus for measuring cytological properties

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2709399A DE2709399C3 (de) 1977-03-04 1977-03-04 Einrichtung zum Messen von Zelleigenschaften

Publications (3)

Publication Number Publication Date
DE2709399A1 DE2709399A1 (de) 1978-09-07
DE2709399B2 DE2709399B2 (de) 1979-11-08
DE2709399C3 true DE2709399C3 (de) 1980-07-24

Family

ID=6002741

Family Applications (1)

Application Number Title Priority Date Filing Date
DE2709399A Expired DE2709399C3 (de) 1977-03-04 1977-03-04 Einrichtung zum Messen von Zelleigenschaften

Country Status (5)

Country Link
US (1) US4225229A (enExample)
CH (1) CH626725A5 (enExample)
DE (1) DE2709399C3 (enExample)
FR (1) FR2382691A1 (enExample)
GB (1) GB1574566A (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4408877A (en) 1979-04-10 1983-10-11 Ernst Leitz Wetzlar Gmbh Device for hydrodynamic focussing of a particle-suspension in a liquid flow cytophotometer

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU938936A1 (ru) * 1978-11-01 1982-06-30 Институт биологической физики АН СССР Устройство дл поиска измененных клеток в цитологическом препарате
US4348107A (en) * 1980-07-18 1982-09-07 Coulter Electronics, Inc. Orifice inside optical element
IT1171329B (it) * 1981-06-25 1987-06-10 B I M Srl Dispositivo per la valutazione obiettiva cella concentrazione e della motilita' degli speramtozoi in campioni di liquido seminale con sistema elettronico di elaborazione e presentazione dei risultati di analisi
DE3233055C2 (de) * 1982-09-06 1994-07-28 Coulter Electronics Durchflußvorrichtung zur Untersuchung einzelner, in einer Flüssigkeit suspendierter Teilchen
US4559299A (en) * 1983-02-04 1985-12-17 Brown University Research Foundation Inc. Cytotoxicity assays in cell culturing devices
DE3420018A1 (de) * 1984-05-29 1985-12-05 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Vorrichtung zur messung bestimmter eigenschaften in einem traegermedium suspendierter partikel
US4661913A (en) * 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
NO156916C (no) * 1985-07-10 1987-12-16 Harald B Steen Stroemningskammer for vaeskestroemsfotometer.
JPH0652263B2 (ja) * 1985-12-10 1994-07-06 株式会社日立製作所 細胞分析装置
EP0286855A1 (de) * 1987-04-15 1988-10-19 BBC Brown Boveri AG Verfahren zum Aetzen von Vertiefungen in ein Siliziumsubstrat
DE3718407A1 (de) * 1987-06-02 1988-12-22 Hund Helmut Gmbh Anordnung fuer die optische analyse von partikelpopulationen in gasen und fluessigkeiten
US4945974A (en) * 1988-02-05 1990-08-07 Reynolds Metals Company Apparatus for and process of direct casting of metal strip
US4954715A (en) * 1989-06-26 1990-09-04 Zoeld Tibor Method and apparatus for an optimized multiparameter flow-through particle and cell analyzer
DE4221063C2 (de) * 1992-06-26 1994-06-01 Thomas Dr Heiden Optisches System für Auflicht-Fluoreszenzmikroskop zur Beobachtung mehrer Fluoreszenzvorgänge
IT1272120B (it) * 1993-03-22 1997-06-11 Bio Rad Spd Srl Camera di misurazione per citometro a flusso
JP4323571B2 (ja) 1997-01-31 2009-09-02 エックスワイ, インコーポレイテッド 光学装置
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6400453B1 (en) 1998-08-21 2002-06-04 Union Biometrica, Inc. Instrument for selecting and depositing multicellular organisms and other large objects
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
WO2002043574A2 (en) 2000-11-29 2002-06-06 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
US8211629B2 (en) 2002-08-01 2012-07-03 Xy, Llc Low pressure sperm cell separation system
CA2534394C (en) 2002-08-15 2013-01-08 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2308419T3 (da) 2003-03-28 2016-06-06 Inguran Llc Fremgangsmåde til bedømmelse af farvningstilstandene for dyresperm, som skal sorteres.
EP1625203B1 (en) 2003-05-15 2015-04-08 Xy, Llc Efficient haploid cell sorting for flow cytometer systems
JP2005030831A (ja) * 2003-07-09 2005-02-03 Aisin Seiki Co Ltd セルソータ用光照射装置
WO2005095590A2 (en) 2004-03-29 2005-10-13 Monsanto Technology Llc Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
AR049732A1 (es) 2004-07-22 2006-08-30 Monsanto Co Proceso para enriquecer una poblacion de celulas de esperma
FI120634B (fi) * 2007-08-17 2009-12-31 Kari Seppaelae Menetelmä ja laitteisto näytteen ottamiseksi
US10900885B2 (en) 2014-12-19 2021-01-26 Captl Llc Flow cytometry using hydrodynamically planar flow
WO2016099538A1 (en) 2014-12-19 2016-06-23 Captl Llc Flow cytometry using hydrodynamically planar flow
DE102020000677B4 (de) 2020-02-01 2023-01-12 Hochschule Anhalt Universaldurchflussmessgerät und Verfahren zur Steuerung eines Universaldurchflussmessgeräts

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1815352C3 (de) * 1968-12-18 1975-03-20 Wolfgang Prof. Dr. Dittrich Automatisches MeB- und Zählgerät für die Teilchen einer Dispersion
US3788744A (en) * 1970-01-14 1974-01-29 Bio Physics Systems Inc Method and apparatus for photoanalysis
US3740665A (en) * 1972-03-16 1973-06-19 Avco Corp Transverse flowing liquid laser
DE7333009U (de) 1972-09-15 1974-03-07 Applied Research Laboratories Ltd Durchflußküvette
US3811777A (en) * 1973-02-06 1974-05-21 Johnson Res Foundation Medical Time-sharing fluorometer and reflectometer
US3984307A (en) * 1973-03-05 1976-10-05 Bio/Physics Systems, Inc. Combined particle sorter and segregation indicator
US3916205A (en) * 1973-05-31 1975-10-28 Block Engineering Differential counting of leukocytes and other cells
US3902807A (en) * 1973-10-25 1975-09-02 Du Pont Method for operating an attenuated total reflection infrared system
US3897154A (en) * 1973-11-15 1975-07-29 Roland C Hawes Sample-background autocancellation in fluid-sample analyzers

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4408877A (en) 1979-04-10 1983-10-11 Ernst Leitz Wetzlar Gmbh Device for hydrodynamic focussing of a particle-suspension in a liquid flow cytophotometer

Also Published As

Publication number Publication date
GB1574566A (en) 1980-09-10
FR2382691B1 (enExample) 1982-07-09
DE2709399A1 (de) 1978-09-07
FR2382691A1 (fr) 1978-09-29
DE2709399B2 (de) 1979-11-08
CH626725A5 (enExample) 1981-11-30
US4225229A (en) 1980-09-30

Similar Documents

Publication Publication Date Title
DE2709399C3 (de) Einrichtung zum Messen von Zelleigenschaften
DE2543310C2 (de) Einrichtung zum Zählen und Klassifizieren von in einer Flüssigkeit suspendierten Teilchen
DE69422908T2 (de) Optische vorrichtung fuer durchflusszytometer
DE69634963T2 (de) Fluorometer
DE69012837T2 (de) Messgerät.
DE2460434A1 (de) Flud-analysator
EP0736767A1 (de) Optische Detektionsvorrichtung für analytische Messungen an chemischen Substanzen
DE1815352A1 (de) Automatisches Mess- und Zaehlgeraet fuer die Teilchen einer Dispersion
EP0941470B1 (de) Fluoreszenzkorrelationsspektroskopiemodul für ein mikroskop
DE881877C (de) Verfahren zur Zaehlung von in einem lichtdurchlaessigen Medium schwebenden Teilchen und Messgeraet zur Durchfuehrung des Verfahrens
DE1797547A1 (de) Vorrichtung zur Messung der Lichtabsorption,insbesondere der Extinktion
DE2710030C3 (de) Vorrichtung zur Photometrierung eines in einer zylindrischen Küvette befindlichen Stoffes
DE3200391A1 (de) Optisches geraet fuer eine fluoreszenzpolarisationsvorrichtung
DE1598667A1 (de) Vorrichtung zur Messung des Raman-Effektes von Proben kleinster Groesse
DE1598420B2 (de) Verfahren und vorrichtung zur bestimmung der menge an freiem ungeloestem wasser in einer fluessigkeit
DE4425462C2 (de) Spektralphotometer-Zelle
DE2742957C2 (de) Mikroskop-Photometer
DE2339575C2 (de) Fotoelektrische Einrichtung zur Herstellung verschiedenartiger Lichtschrankensysteme
DE3603905A1 (de) Durchfluss-teilchenanalysator und durchflusszelle fuer diesen
DE3318376C1 (de) Vorrichtung zur Anregung von Fotolumineszenz und zur Beobachtung derselben
DE10131724B4 (de) Optisches Absorptions-Messgerät
DE2320937B2 (de) Optische Vorrichtung zum Aufnehmen der Lichtabsorption in einem Objekt, das außerdem einen veränderlichen Brechungskoeffizienten aufweist
EP0950893A2 (de) Vorrichtung zum Nachweis eines Fluoreszenzfarbstoffs
DE3718407A1 (de) Anordnung fuer die optische analyse von partikelpopulationen in gasen und fluessigkeiten
DE3881722T2 (de) Analysegerät für UV-Fluoreszenz.

Legal Events

Date Code Title Description
OAP Request for examination filed
OD Request for examination
C3 Grant after two publication steps (3rd publication)
8339 Ceased/non-payment of the annual fee