FR2382691A1 - Dispositif de mesure de proprietes de cellules - Google Patents

Dispositif de mesure de proprietes de cellules

Info

Publication number
FR2382691A1
FR2382691A1 FR7806137A FR7806137A FR2382691A1 FR 2382691 A1 FR2382691 A1 FR 2382691A1 FR 7806137 A FR7806137 A FR 7806137A FR 7806137 A FR7806137 A FR 7806137A FR 2382691 A1 FR2382691 A1 FR 2382691A1
Authority
FR
France
Prior art keywords
channel
measuring
measuring points
cell properties
measuring cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7806137A
Other languages
English (en)
Other versions
FR2382691B1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of FR2382691A1 publication Critical patent/FR2382691A1/fr
Application granted granted Critical
Publication of FR2382691B1 publication Critical patent/FR2382691B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Optical Measuring Cells (AREA)

Abstract

L'invention a trait à un dispositif de mesure des propriétés de cellules. Elle a pour objet un dispositif de mesure de cellules en suspension dans un liquide et passant avec celui-ci par des points de mesure réalises dans un canal d'une chambre d'écoulement et pouvant être examinés à travers une fenêtre de la paroi du canal, par excitation de fluorescence à l'aide de deux sources lumineuses de longueurs d'onde différentes, une source de lumière étant associée à un point de mesure. Les points de mesure 20, 22 sont réalisés en un coude 12 du canal d'écoulement, la section d'arrivée et la section de départ formant chacun un des points de mesure séparés par l'angle inclus du coude 16, 18. Application : analyses biologiques.
FR7806137A 1977-03-04 1978-03-03 Dispositif de mesure de proprietes de cellules Granted FR2382691A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2709399A DE2709399C3 (de) 1977-03-04 1977-03-04 Einrichtung zum Messen von Zelleigenschaften

Publications (2)

Publication Number Publication Date
FR2382691A1 true FR2382691A1 (fr) 1978-09-29
FR2382691B1 FR2382691B1 (fr) 1982-07-09

Family

ID=6002741

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7806137A Granted FR2382691A1 (fr) 1977-03-04 1978-03-03 Dispositif de mesure de proprietes de cellules

Country Status (5)

Country Link
US (1) US4225229A (fr)
CH (1) CH626725A5 (fr)
DE (1) DE2709399C3 (fr)
FR (1) FR2382691A1 (fr)
GB (1) GB1574566A (fr)

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SU938936A1 (ru) * 1978-11-01 1982-06-30 Институт биологической физики АН СССР Устройство дл поиска измененных клеток в цитологическом препарате
US4348107A (en) * 1980-07-18 1982-09-07 Coulter Electronics, Inc. Orifice inside optical element
IT1171329B (it) * 1981-06-25 1987-06-10 B I M Srl Dispositivo per la valutazione obiettiva cella concentrazione e della motilita' degli speramtozoi in campioni di liquido seminale con sistema elettronico di elaborazione e presentazione dei risultati di analisi
DE3233055C2 (de) * 1982-09-06 1994-07-28 Coulter Electronics Durchflußvorrichtung zur Untersuchung einzelner, in einer Flüssigkeit suspendierter Teilchen
US4559299A (en) * 1983-02-04 1985-12-17 Brown University Research Foundation Inc. Cytotoxicity assays in cell culturing devices
DE3420018A1 (de) * 1984-05-29 1985-12-05 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Vorrichtung zur messung bestimmter eigenschaften in einem traegermedium suspendierter partikel
US4661913A (en) * 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
NO156916C (no) * 1985-07-10 1987-12-16 Harald B Steen Stroemningskammer for vaeskestroemsfotometer.
JPH0652263B2 (ja) * 1985-12-10 1994-07-06 株式会社日立製作所 細胞分析装置
EP0286855A1 (fr) * 1987-04-15 1988-10-19 BBC Brown Boveri AG Procédé pour creuser des sillons dans un substrat de silicium
DE3718407A1 (de) * 1987-06-02 1988-12-22 Hund Helmut Gmbh Anordnung fuer die optische analyse von partikelpopulationen in gasen und fluessigkeiten
US4945974A (en) * 1988-02-05 1990-08-07 Reynolds Metals Company Apparatus for and process of direct casting of metal strip
US4954715A (en) * 1989-06-26 1990-09-04 Zoeld Tibor Method and apparatus for an optimized multiparameter flow-through particle and cell analyzer
DE4221063C2 (de) * 1992-06-26 1994-06-01 Thomas Dr Heiden Optisches System für Auflicht-Fluoreszenzmikroskop zur Beobachtung mehrer Fluoreszenzvorgänge
IT1272120B (it) * 1993-03-22 1997-06-11 Bio Rad Spd Srl Camera di misurazione per citometro a flusso
JP4323571B2 (ja) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド 光学装置
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
AU754644B2 (en) 1998-08-21 2002-11-21 Union Biometrica, Inc. Instrument for selecting and depositing multicellular organisms and other large objects
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
CA2822983C (fr) 2000-11-29 2017-05-09 Xy, Llc Systeme de dissociation de spermatozoides congeles-decongeles porteurs d'un chromosome x de ceux porteurs d'un chromosome y
BRPI0313163B1 (pt) 2002-08-01 2015-11-17 Univ Colorado State sistema de separação de células espermáticas a baixa pressão
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
AU2003265471B2 (en) 2002-08-15 2009-08-06 Xy, Llc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2305173T3 (en) 2003-03-28 2016-08-22 Inguran Llc Apparatus and method for providing sorted particles
US20060263829A1 (en) 2003-05-15 2006-11-23 Evans Kenneth M Efficient haploid cell sorting flow cytometer systems
JP2005030831A (ja) * 2003-07-09 2005-02-03 Aisin Seiki Co Ltd セルソータ用光照射装置
AU2005229073B2 (en) 2004-03-29 2010-08-19 Inguran, Llc Sperm suspensions for use in insemination
US7833147B2 (en) 2004-07-22 2010-11-16 Inguran, LLC. Process for enriching a population of sperm cells
FI120634B (fi) * 2007-08-17 2009-12-31 Kari Seppaelae Menetelmä ja laitteisto näytteen ottamiseksi
WO2016099538A1 (fr) 2014-12-19 2016-06-23 Captl Llc Cytométrie en flux utilisant un écoulement plan du point de vue hydrodynamique
WO2016100954A1 (fr) 2014-12-19 2016-06-23 Captl Llc Cytométrie en flux utilisant un flux planaire de manière hydrodynamique
DE102020000677B4 (de) 2020-02-01 2023-01-12 Hochschule Anhalt Universaldurchflussmessgerät und Verfahren zur Steuerung eines Universaldurchflussmessgeräts

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3761187A (en) * 1968-12-18 1973-09-25 W Dittrich Flow-through chamber for photometers to measure and count particles in a dispersion medium

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3788744A (en) * 1970-01-14 1974-01-29 Bio Physics Systems Inc Method and apparatus for photoanalysis
US3740665A (en) * 1972-03-16 1973-06-19 Avco Corp Transverse flowing liquid laser
US3811777A (en) * 1973-02-06 1974-05-21 Johnson Res Foundation Medical Time-sharing fluorometer and reflectometer
US3984307A (en) * 1973-03-05 1976-10-05 Bio/Physics Systems, Inc. Combined particle sorter and segregation indicator
US3916205A (en) * 1973-05-31 1975-10-28 Block Engineering Differential counting of leukocytes and other cells
US3902807A (en) * 1973-10-25 1975-09-02 Du Pont Method for operating an attenuated total reflection infrared system
US3897154A (en) * 1973-11-15 1975-07-29 Roland C Hawes Sample-background autocancellation in fluid-sample analyzers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3761187A (en) * 1968-12-18 1973-09-25 W Dittrich Flow-through chamber for photometers to measure and count particles in a dispersion medium

Also Published As

Publication number Publication date
DE2709399B2 (de) 1979-11-08
DE2709399A1 (de) 1978-09-07
CH626725A5 (fr) 1981-11-30
US4225229A (en) 1980-09-30
FR2382691B1 (fr) 1982-07-09
DE2709399C3 (de) 1980-07-24
GB1574566A (en) 1980-09-10

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