DE2661010C2 - Immunologisches Reagens und nephelometrisches Untersuchungsverfahren - Google Patents
Immunologisches Reagens und nephelometrisches UntersuchungsverfahrenInfo
- Publication number
- DE2661010C2 DE2661010C2 DE2661010A DE2661010A DE2661010C2 DE 2661010 C2 DE2661010 C2 DE 2661010C2 DE 2661010 A DE2661010 A DE 2661010A DE 2661010 A DE2661010 A DE 2661010A DE 2661010 C2 DE2661010 C2 DE 2661010C2
- Authority
- DE
- Germany
- Prior art keywords
- reagent
- antigen
- antibody
- assay
- nephelometric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 34
- 238000003556 assay Methods 0.000 title claims description 23
- 230000001900 immune effect Effects 0.000 title description 7
- 239000000427 antigen Substances 0.000 claims description 17
- 102000036639 antigens Human genes 0.000 claims description 17
- 108091007433 antigens Proteins 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 12
- -1 polyoxypropylene Polymers 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 7
- 238000010324 immunological assay Methods 0.000 claims description 6
- 229920001451 polypropylene glycol Polymers 0.000 claims description 6
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 5
- 229920001400 block copolymer Polymers 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 229920000642 polymer Polymers 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000004458 analytical method Methods 0.000 description 10
- 239000002202 Polyethylene glycol Substances 0.000 description 7
- 239000002245 particle Substances 0.000 description 7
- 229920001223 polyethylene glycol Polymers 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 4
- 239000002736 nonionic surfactant Substances 0.000 description 4
- 229920001983 poloxamer Polymers 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 229920005862 polyol Polymers 0.000 description 3
- 150000003077 polyols Chemical class 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 2
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- GVNVAWHJIKLAGL-UHFFFAOYSA-N 2-(cyclohexen-1-yl)cyclohexan-1-one Chemical compound O=C1CCCCC1C1=CCCCC1 GVNVAWHJIKLAGL-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 101150065749 Churc1 gene Proteins 0.000 description 1
- 102000016918 Complement C3 Human genes 0.000 description 1
- 108010028780 Complement C3 Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000006771 Gonadotropins Human genes 0.000 description 1
- 108010086677 Gonadotropins Proteins 0.000 description 1
- 102000014702 Haptoglobin Human genes 0.000 description 1
- 108050005077 Haptoglobin Proteins 0.000 description 1
- 102000007330 LDL Lipoproteins Human genes 0.000 description 1
- 108010007622 LDL Lipoproteins Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229920002012 Pluronic® F 38 Polymers 0.000 description 1
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 1
- 102100038239 Protein Churchill Human genes 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 102000011923 Thyrotropin Human genes 0.000 description 1
- 108010061174 Thyrotropin Proteins 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000002622 gonadotropin Substances 0.000 description 1
- 229940094892 gonadotropins Drugs 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004848 nephelometry Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000013618 particulate matter Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54506675A | 1975-01-29 | 1975-01-29 | |
| US55590875A | 1975-03-06 | 1975-03-06 | |
| US05/615,024 US4148869A (en) | 1975-03-06 | 1975-09-19 | Immunological reagent and method of using same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2661010C2 true DE2661010C2 (de) | 1987-03-19 |
Family
ID=27415448
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2661010A Expired DE2661010C2 (de) | 1975-01-29 | 1976-01-21 | Immunologisches Reagens und nephelometrisches Untersuchungsverfahren |
| DE2602068A Expired DE2602068C2 (de) | 1975-01-29 | 1976-01-21 | Reagens zur Unlöslichmachung eines Antigen/Antikörper-Komplexes |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2602068A Expired DE2602068C2 (de) | 1975-01-29 | 1976-01-21 | Reagens zur Unlöslichmachung eines Antigen/Antikörper-Komplexes |
Country Status (15)
| Country | Link |
|---|---|
| JP (1) | JPS604938B2 (xx) |
| BE (1) | BE837675A (xx) |
| BR (1) | BR7600322A (xx) |
| CA (1) | CA1080619A (xx) |
| CH (1) | CH613047A5 (xx) |
| DE (2) | DE2661010C2 (xx) |
| DK (1) | DK150809C (xx) |
| ES (1) | ES444543A1 (xx) |
| FR (1) | FR2299645A1 (xx) |
| GB (1) | GB1540097A (xx) |
| IL (1) | IL48804A (xx) |
| IT (1) | IT1062433B (xx) |
| NL (1) | NL183852C (xx) |
| NO (1) | NO149864C (xx) |
| SE (1) | SE441392B (xx) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2901327B1 (de) * | 1979-01-15 | 1980-07-24 | Boehringer Mannheim Gmbh | Verfahren zur Bestimmung eines opsonierenden oberflaechenbindenden alpha?-Glykoproteins |
| FI792576A (fi) * | 1979-08-20 | 1981-02-21 | Orion Yhtymae Oy | Foerbaettrad metod att utfoera enzymimmunologiska bestaemningar |
| JPS5943362A (ja) * | 1982-09-06 | 1984-03-10 | Kainosu:Kk | 免疫学的測定試薬 |
| AU580248B2 (en) * | 1982-10-13 | 1989-01-12 | Biowhittaker Technologies, Inc. | Fluorometric assay of allergic reactions and reagents therefor |
| GB8317855D0 (en) * | 1983-06-30 | 1983-08-03 | Iq Bio Ltd | Biochemical detection method |
| DE3327642A1 (de) * | 1983-07-30 | 1985-02-14 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur bestimmung eines partners einer immunreaktion sowie reagens zur durchfuehrung dieses verfahrens |
| DE3532626A1 (de) * | 1985-09-12 | 1987-03-19 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung eines partners einer immunreaktion |
| JPS62159047A (ja) * | 1985-12-31 | 1987-07-15 | Chemo Sero Therapeut Res Inst | 血漿蛋白定量用試薬 |
| US4810638A (en) * | 1986-07-24 | 1989-03-07 | Miles Inc. | Enzyme-labeled antibody reagent with polyalkyleneglycol linking group |
| JP2684069B2 (ja) * | 1988-10-13 | 1997-12-03 | 昇一 首藤 | 免疫学的活性物質の測定方法及びそのための試薬 |
| CA2077906A1 (en) * | 1991-01-10 | 1992-07-11 | Kimihiko Matsuzawa | Method for measurement of tissue factor in high sensitivity and measurement kit therefor |
| EP0642665A4 (en) * | 1993-03-26 | 1997-05-02 | Curative Tech Inc | METHOD AND ASSAY INCLUDING IMPROVED BINDING REACTIVITY OF A POLYPEPTIDE. |
| EP1617277B1 (en) * | 2003-04-03 | 2009-05-27 | Seed Co., Ltd. | Ophthalmic lenses capable of sustained drug release and preservative solutions therefor |
| JP4915638B2 (ja) * | 2005-08-26 | 2012-04-11 | パナソニック株式会社 | 無電極放電灯装置及びこの無電極放電灯装置を備えた照明器具 |
| JP6242068B2 (ja) * | 2013-04-10 | 2017-12-06 | 積水メディカル株式会社 | ラテックス免疫凝集測定試薬 |
| TWI842764B (zh) * | 2018-11-09 | 2024-05-21 | 日商積水醫療股份有限公司 | 抑制以自動分析裝置進行的免疫測定中之異常檢測的方法、檢測方法、免疫測定試劑、及免疫測定試劑套組 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1598151A1 (de) * | 1966-11-19 | 1970-05-27 | Kleine Dr Rer Nat Norbert | Agglutinationsverstaerker |
| DE2361169C3 (de) * | 1973-12-07 | 1978-09-14 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur Aktivierung von von Detengensspuren befreiter Cholesterinoxydase |
| US3947250A (en) * | 1974-06-21 | 1976-03-30 | Baxter Laboratories, Inc. | Method of immunodiffusion |
-
1976
- 1976-01-07 IL IL48804A patent/IL48804A/xx unknown
- 1976-01-12 CA CA243,325A patent/CA1080619A/en not_active Expired
- 1976-01-12 SE SE7600232A patent/SE441392B/xx not_active IP Right Cessation
- 1976-01-15 FR FR7600986A patent/FR2299645A1/fr active Granted
- 1976-01-16 IT IT19348/76A patent/IT1062433B/it active
- 1976-01-16 GB GB1784/76A patent/GB1540097A/en not_active Expired
- 1976-01-16 DK DK016976A patent/DK150809C/da not_active IP Right Cessation
- 1976-01-19 BR BR7600322A patent/BR7600322A/pt unknown
- 1976-01-19 BE BE163598A patent/BE837675A/xx not_active IP Right Cessation
- 1976-01-21 DE DE2661010A patent/DE2661010C2/de not_active Expired
- 1976-01-21 DE DE2602068A patent/DE2602068C2/de not_active Expired
- 1976-01-22 ES ES444543A patent/ES444543A1/es not_active Expired
- 1976-01-22 NO NO760203A patent/NO149864C/no unknown
- 1976-01-23 NL NLAANVRAGE7600697,A patent/NL183852C/xx not_active IP Right Cessation
- 1976-01-23 JP JP51007067A patent/JPS604938B2/ja not_active Expired
- 1976-01-27 CH CH102276A patent/CH613047A5/xx not_active IP Right Cessation
Non-Patent Citations (2)
| Title |
|---|
| Brit.Med.Bull., Vol. 30, No. 1 1974, S. 32-37 * |
| Clin.Chem., Vol. 20, No. 8, 1974, S. 1055-1061 * |
Also Published As
| Publication number | Publication date |
|---|---|
| DK150809C (da) | 1988-05-02 |
| BE837675A (fr) | 1976-07-19 |
| NL183852C (nl) | 1989-02-01 |
| BR7600322A (pt) | 1976-08-31 |
| IL48804A (en) | 1979-05-31 |
| CA1080619A (en) | 1980-07-01 |
| NO760203L (xx) | 1976-07-30 |
| JPS604938B2 (ja) | 1985-02-07 |
| DK16976A (da) | 1976-07-30 |
| NL7600697A (nl) | 1976-08-02 |
| NO149864C (no) | 1984-07-04 |
| FR2299645B1 (xx) | 1980-11-28 |
| SE7600232L (sv) | 1976-07-30 |
| DE2602068A1 (de) | 1976-08-05 |
| IL48804A0 (en) | 1976-03-31 |
| FR2299645A1 (fr) | 1976-08-27 |
| CH613047A5 (en) | 1979-08-31 |
| SE441392B (sv) | 1985-09-30 |
| NO149864B (no) | 1984-03-26 |
| ES444543A1 (es) | 1977-06-01 |
| NL183852B (nl) | 1988-09-01 |
| IT1062433B (it) | 1984-10-10 |
| JPS51101121A (xx) | 1976-09-07 |
| DE2602068C2 (de) | 1986-09-11 |
| DK150809B (da) | 1987-06-22 |
| GB1540097A (en) | 1979-02-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| Q172 | Divided out of (supplement): |
Ref country code: DE Ref document number: 2602068 |
|
| 8110 | Request for examination paragraph 44 | ||
| 8181 | Inventor (new situation) |
Free format text: DEATON, CARLTON, GARDEN GROVE, CALIF., US ACHTER, EUGENE, GAITHERSBURG, MARYLAND, US KREMEN, JEROME, TAKOMA PARK, MARYLAND, US RODRIGUEZ, RODOLFO, COLUMBIA, MARYLAND, US PRIARONE, PAUL, W. HYATTSWILLE, MARYLAND, US |
|
| AC | Divided out of |
Ref country code: DE Ref document number: 2602068 Format of ref document f/p: P |
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| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: TECHNICON INSTRUMENTS CORP. (N.D.GES.D.STAATES DEL |