DE2537098A1 - Verfahren und vorrichtung zur unterscheidung zwischen zwei fluoreszierenden teilchenarten mit unterschiedlichen fluoreszenzabklingdauern - Google Patents
Verfahren und vorrichtung zur unterscheidung zwischen zwei fluoreszierenden teilchenarten mit unterschiedlichen fluoreszenzabklingdauernInfo
- Publication number
- DE2537098A1 DE2537098A1 DE19752537098 DE2537098A DE2537098A1 DE 2537098 A1 DE2537098 A1 DE 2537098A1 DE 19752537098 DE19752537098 DE 19752537098 DE 2537098 A DE2537098 A DE 2537098A DE 2537098 A1 DE2537098 A1 DE 2537098A1
- Authority
- DE
- Germany
- Prior art keywords
- intensity
- radiation
- fluorescence
- fluorescence emission
- types
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002245 particle Substances 0.000 title claims description 38
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- 125000003545 alkoxy group Chemical group 0.000 description 6
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- 230000005281 excited state Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- 241001274216 Naso Species 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 210000003979 eosinophil Anatomy 0.000 description 2
- 229910052736 halogen Chemical class 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- TXVWTOBHDDIASC-UHFFFAOYSA-N 1,2-diphenylethene-1,2-diamine Chemical compound C=1C=CC=CC=1C(N)=C(N)C1=CC=CC=C1 TXVWTOBHDDIASC-UHFFFAOYSA-N 0.000 description 1
- AMYULKAMZOQMRS-UHFFFAOYSA-N 10-ethyl-9-phenylphenanthridine-2,7-diamine Chemical compound C1=C(N)C2=CN=C3C=CC(N)=CC3=C2C(CC)=C1C1=CC=CC=C1 AMYULKAMZOQMRS-UHFFFAOYSA-N 0.000 description 1
- FBAQFBBASACECK-UHFFFAOYSA-N 2-amino-6-[2-(2-sulfophenyl)ethenyl]benzenesulfonic acid Chemical compound NC1=CC=CC(C=CC=2C(=CC=CC=2)S(O)(=O)=O)=C1S(O)(=O)=O FBAQFBBASACECK-UHFFFAOYSA-N 0.000 description 1
- ZAJAQTYSTDTMCU-UHFFFAOYSA-N 3-aminobenzenesulfonic acid Chemical compound NC1=CC=CC(S(O)(=O)=O)=C1 ZAJAQTYSTDTMCU-UHFFFAOYSA-N 0.000 description 1
- WXAIEIRYBSKHDP-UHFFFAOYSA-N 4-phenyl-n-(4-phenylphenyl)-n-[4-[4-(4-phenyl-n-(4-phenylphenyl)anilino)phenyl]phenyl]aniline Chemical compound C1=CC=CC=C1C1=CC=C(N(C=2C=CC(=CC=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)C=2C=CC(=CC=2)N(C=2C=CC(=CC=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)C=2C=CC=CC=2)C=C1 WXAIEIRYBSKHDP-UHFFFAOYSA-N 0.000 description 1
- OBJOZRVSMLPASY-UHFFFAOYSA-N 8-hydroxypyrene-1,3,6-trisulfonic acid Chemical class C1=C2C(O)=CC(S(O)(=O)=O)=C(C=C3)C2=C2C3=C(S(O)(=O)=O)C=C(S(O)(=O)=O)C2=C1 OBJOZRVSMLPASY-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- PJANXHGTPQOBST-VAWYXSNFSA-N Stilbene Natural products C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 125000002490 anilino group Chemical group [H]N(*)C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- XJHABGPPCLHLLV-UHFFFAOYSA-N benzo[de]isoquinoline-1,3-dione Chemical class C1=CC(C(=O)NC2=O)=C3C2=CC=CC3=C1 XJHABGPPCLHLLV-UHFFFAOYSA-N 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000000983 mordant dye Substances 0.000 description 1
- PSZYNBSKGUBXEH-UHFFFAOYSA-N naphthalene-1-sulfonic acid Chemical compound C1=CC=C2C(S(=O)(=O)O)=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000002894 organic compounds Chemical group 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 238000006303 photolysis reaction Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical compound C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 235000015149 toffees Nutrition 0.000 description 1
- 125000004306 triazinyl group Chemical group 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US49913274A | 1974-08-21 | 1974-08-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2537098A1 true DE2537098A1 (de) | 1976-03-04 |
Family
ID=23983956
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752537098 Pending DE2537098A1 (de) | 1974-08-21 | 1975-08-20 | Verfahren und vorrichtung zur unterscheidung zwischen zwei fluoreszierenden teilchenarten mit unterschiedlichen fluoreszenzabklingdauern |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS5149790A (enExample) |
| AU (1) | AU8396175A (enExample) |
| DE (1) | DE2537098A1 (enExample) |
| FR (1) | FR2282639A1 (enExample) |
| GB (1) | GB1476006A (enExample) |
| NL (1) | NL7509921A (enExample) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4365153A (en) | 1979-06-25 | 1982-12-21 | Scintrex Limited | Detection of certain minerals of zinc, tungsten, fluorine, molybdenum, mercury and other metals using photoluminescence |
| DE3614359A1 (de) * | 1985-07-26 | 1987-02-05 | Jenoptik Jena Gmbh | Anordnung zur bildlichen darstellung und analyse von fluoreszenzsignalen |
| DE4137008A1 (de) * | 1991-11-11 | 1993-05-13 | Heribert F Dr Ing Broicher | Vorrichtung zur feststellung von qualitaetsaenderungen von massenguetern auf laufenden foerderbaendern |
| DE4210970A1 (de) * | 1992-04-02 | 1993-10-07 | Markus Dipl Chem Sauer | Verfahren zur optischen qualitativen und quantitativen Erfassung von Biomolekülen, toxischen Substanzen, Polymeren und pharmazeutischen Wirkstoffen mittels Laserspektroskopie |
| DE4344201A1 (de) * | 1993-12-23 | 1995-07-06 | Sauer Rolf Prof Dr | Verfahren zur zeitaufgelösten Messung von physikalischen Abklingprozessen |
| DE4401351A1 (de) * | 1994-01-14 | 1995-07-20 | Werec Gmbh Berlin Wertstoff Re | Verfahren und Vorrichtung zur Identifizierung lumineszierender Substanzen in Gefäßen, insbesondere in Entladungslampen |
| DE19507119A1 (de) * | 1994-03-01 | 1995-09-07 | Wolfgang Schade | Vorrichtung zur Bestimmung von Verunreinigungen |
| DE19802378A1 (de) * | 1997-01-30 | 1998-08-06 | Hewlett Packard Co | Vorrichtung und Verfahren zur Abtastung eines chemischen Arrays |
| DE10314848A1 (de) * | 2002-06-28 | 2004-01-15 | Jenoptik Laser, Optik, Systeme Gmbh | Messeinrichtung zur Detektion von laserinduzierter Fluoreszenzstrahlung |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2374636A1 (fr) * | 1976-12-14 | 1978-07-13 | Block Engineering | Systeme photometrique pour examiner des materiaux fluorescents |
| JPS59184315A (ja) * | 1983-04-02 | 1984-10-19 | Rikagaku Kenkyusho | 標本の状態検出方法とそのためのレ−ザ−顕微鏡 |
| US4877965A (en) * | 1985-07-01 | 1989-10-31 | Diatron Corporation | Fluorometer |
| SE0003796D0 (sv) * | 2000-10-20 | 2000-10-20 | Astrazeneca Ab | Apparatus and method for monitoring |
| JP4300366B2 (ja) * | 2005-03-30 | 2009-07-22 | 独立行政法人産業技術総合研究所 | 蛍光検出方法、蛍光検出用ビーズの作製方法及び蛍光検出用ビーズ |
| JP4399575B2 (ja) * | 2005-03-31 | 2010-01-20 | 独立行政法人産業技術総合研究所 | 遺伝子断片中のヌクレオチドの種類の特定方法 |
-
1975
- 1975-08-14 AU AU83961/75A patent/AU8396175A/en not_active Expired
- 1975-08-18 GB GB3425375A patent/GB1476006A/en not_active Expired
- 1975-08-20 FR FR7525792A patent/FR2282639A1/fr active Granted
- 1975-08-20 DE DE19752537098 patent/DE2537098A1/de active Pending
- 1975-08-21 JP JP10166975A patent/JPS5149790A/ja active Pending
- 1975-08-21 NL NL7509921A patent/NL7509921A/xx not_active Application Discontinuation
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4365153A (en) | 1979-06-25 | 1982-12-21 | Scintrex Limited | Detection of certain minerals of zinc, tungsten, fluorine, molybdenum, mercury and other metals using photoluminescence |
| DE3614359A1 (de) * | 1985-07-26 | 1987-02-05 | Jenoptik Jena Gmbh | Anordnung zur bildlichen darstellung und analyse von fluoreszenzsignalen |
| DE4137008A1 (de) * | 1991-11-11 | 1993-05-13 | Heribert F Dr Ing Broicher | Vorrichtung zur feststellung von qualitaetsaenderungen von massenguetern auf laufenden foerderbaendern |
| DE4210970A1 (de) * | 1992-04-02 | 1993-10-07 | Markus Dipl Chem Sauer | Verfahren zur optischen qualitativen und quantitativen Erfassung von Biomolekülen, toxischen Substanzen, Polymeren und pharmazeutischen Wirkstoffen mittels Laserspektroskopie |
| DE4344201A1 (de) * | 1993-12-23 | 1995-07-06 | Sauer Rolf Prof Dr | Verfahren zur zeitaufgelösten Messung von physikalischen Abklingprozessen |
| DE4401351A1 (de) * | 1994-01-14 | 1995-07-20 | Werec Gmbh Berlin Wertstoff Re | Verfahren und Vorrichtung zur Identifizierung lumineszierender Substanzen in Gefäßen, insbesondere in Entladungslampen |
| DE19507119A1 (de) * | 1994-03-01 | 1995-09-07 | Wolfgang Schade | Vorrichtung zur Bestimmung von Verunreinigungen |
| DE19802378A1 (de) * | 1997-01-30 | 1998-08-06 | Hewlett Packard Co | Vorrichtung und Verfahren zur Abtastung eines chemischen Arrays |
| US5945679A (en) * | 1997-01-30 | 1999-08-31 | Hewlett-Packard Company | Apparatus for scanning a chemical array |
| DE19802378C2 (de) * | 1997-01-30 | 2000-07-13 | Hewlett Packard Co | Vorrichtung und Verfahren zum Analysieren von Chemikalien in einem Array mit einer Mehrzahl von Arrayelementen |
| DE10314848A1 (de) * | 2002-06-28 | 2004-01-15 | Jenoptik Laser, Optik, Systeme Gmbh | Messeinrichtung zur Detektion von laserinduzierter Fluoreszenzstrahlung |
Also Published As
| Publication number | Publication date |
|---|---|
| AU8396175A (en) | 1977-02-17 |
| GB1476006A (en) | 1977-06-10 |
| JPS5149790A (ja) | 1976-04-30 |
| NL7509921A (nl) | 1976-02-24 |
| FR2282639A1 (fr) | 1976-03-19 |
| FR2282639B3 (enExample) | 1978-04-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHJ | Non-payment of the annual fee |